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Protein

Serine/threonine-protein kinase MRCK gamma

Gene

Cdc42bpg

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+By similarity

Enzyme regulationi

Maintained in an inactive, closed conformation by an interaction between the kinase domain and the negative autoregulatory C-terminal coiled-coil region. Agonist binding to the phorbol ester binding site disrupts this, releasing the kinase domain to allow N-terminus-mediated dimerization and kinase activation by transautophosphorylation (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei100 – 1001ATPPROSITE-ProRule annotationBy similarity
Active sitei195 – 1951Proton acceptorPROSITE-ProRule annotationBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi77 – 859ATPPROSITE-ProRule annotationBy similarity
Zinc fingeri877 – 92650Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase MRCK gamma (EC:2.7.11.1)
Alternative name(s):
CDC42-binding protein kinase gamma
DMPK-like gamma
Myotonic dystrophy kinase-related CDC42-binding kinase gamma
Short name:
MRCK gamma
Short name:
Myotonic dystrophy protein kinase-like gamma
Myotonic dystrophy protein kinase-like alpha
Gene namesi
Name:Cdc42bpgImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2652845. Cdc42bpg.

Subcellular locationi

  • Cytoplasm By similarity

  • Note: Concentrates at the leading edge of cells.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15511551Serine/threonine-protein kinase MRCK gammaPRO_0000086398Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei216 – 2161Phosphoserine; by autocatalysisBy similarity
Modified residuei228 – 2281Phosphoserine; by autocatalysisBy similarity
Modified residuei234 – 2341Phosphothreonine; by autocatalysisBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ80UW5.
PaxDbiQ80UW5.
PRIDEiQ80UW5.

PTM databases

PhosphoSiteiQ80UW5.

Expressioni

Gene expression databases

BgeeiQ80UW5.
CleanExiMM_CDC42BPG.
GenevisibleiQ80UW5. MM.

Interactioni

Subunit structurei

Homodimer and homotetramer via the coiled coil regions. Interacts tightly with GTP-bound but not GDP-bound CDC42 (By similarity).By similarity

Protein-protein interaction databases

BioGridi232205. 1 interaction.
IntActiQ80UW5. 1 interaction.
STRINGi10090.ENSMUSP00000025681.

Structurei

3D structure databases

ProteinModelPortaliQ80UW5.
SMRiQ80UW5. Positions 2-400.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini71 – 337267Protein kinasePROSITE-ProRule annotationBy similarityAdd
BLAST
Domaini338 – 40871AGC-kinase C-terminalAdd
BLAST
Domaini946 – 1065120PHPROSITE-ProRule annotationAdd
BLAST
Domaini1091 – 1365275CNHPROSITE-ProRule annotationAdd
BLAST
Domaini1436 – 144914CRIBPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili442 – 675234Sequence analysisAdd
BLAST
Coiled coili729 – 80173Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1289 – 12924Poly-LeuSequence analysis

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 CNH domain.PROSITE-ProRule annotation
Contains 1 CRIB domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri877 – 92650Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0612. Eukaryota.
ENOG410XR1Q. LUCA.
GeneTreeiENSGT00760000118994.
HOGENOMiHOG000294133.
HOVERGENiHBG055933.
InParanoidiQ80UW5.
KOiK16307.
OMAiFRNHPFF.
OrthoDBiEOG7F511X.
PhylomeDBiQ80UW5.
TreeFamiTF313551.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR001180. CNH_dom.
IPR000095. CRIB_dom.
IPR011009. Kinase-like_dom.
IPR033235. MRCK_gamma.
IPR014930. Myotonic_dystrophy_kinase_coil.
IPR002219. PE/DAG-bd.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR22988:SF22. PTHR22988:SF22. 1 hit.
PfamiPF00130. C1_1. 1 hit.
PF00780. CNH. 1 hit.
PF08826. DMPK_coil. 1 hit.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
ProDomiPD011252. Myotonic_dystrophy_kinase_coil. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00109. C1. 1 hit.
SM00036. CNH. 1 hit.
SM00285. PBD. 1 hit.
SM00233. PH. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50219. CNH. 1 hit.
PS50108. CRIB. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q80UW5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQRLRALEQ LVRGEAGGSP GLDGLLDLLL GVHQELSSAP LRRERNVAQF
60 70 80 90 100
LSWASPFVTK VKELRLQRDD FEILKVIGRG AFGEVAVVRQ RGSGQIFAMK
110 120 130 140 150
MLHKWEMLKR AETACFREER DVLVKGDSRW VTALHYAFQD EEYLYLVMDY
160 170 180 190 200
YAGGDLLTLL SRFEDRLPPE LAQFYLAEMV LAIHSLHQLG YVHRDVKPDN
210 220 230 240 250
ILLDMNGHIR LADFGSCLRL NNNGMVDSSV AVGTPDYISP EILQAMEEGK
260 270 280 290 300
GHYGPQCDWW SLGVCAYELL FGETPFYAES LVETYGKIMN HEDHLQFPAD
310 320 330 340 350
VTDVPASAQD LIRQLLCRQE ERLGRGGLDD FRKHPFFEGV DWERLATSTA
360 370 380 390 400
PYIPELRGPM DTSNFDVDDD TLNRPETLPP SSHGAFSGHH LPFVGFTYTS
410 420 430 440 450
GSPFDVQSSE LMAAPEGTPH CVEQVKVELS HKCQEPLHGP LQPQELVRLQ
460 470 480 490 500
KEVQVLQEKL AETLRDSKAS LSQTDGLHAR SPAPNIQLQQ EKDRLQQELT
510 520 530 540 550
EAQAALRVQD AELCQAQNRQ EEFLQRLWEA QEREAAAASQ IQALNSQLEE
560 570 580 590 600
AWVVRRELEG QVTTLSQEVT RLQGQCKQES SQAKTVHAAP ETNGIGSPEG
610 620 630 640 650
QSQEAQLRKE VAALREQLEH ACSQGISVGK EEVLCRLQEE NQRLSREQER
660 670 680 690 700
LAGELELELQ SKQRLEGERR ETESNWEAQI ADILSWVNDE KVSRGYLQAL
710 720 730 740 750
ATKMAEELES LRNVGTQTLP TRPLDHQWKA RRLQKMEASA RLELQSALEA
760 770 780 790 800
EIRAKQSLQE QLTQVQEAQR QAERRLQEAE KQSQALQQEV AELREELQAR
810 820 830 840 850
GPGDARPSTS LIPLLSFWNT EKDSAKDPGN SGEGPRSGAE AELRPEGRRS
860 870 880 890 900
LRMGSVFPRV PAATTTPAEG PPAKPGSHTL RPRSFPSPTK CLRCTSLMLG
910 920 930 940 950
LGRQGLGCDT CGYFCHSACA SQAPPCPVPP ELLRTALGVH PETGTGTAYE
960 970 980 990 1000
GFLSVPRPSG VRRGWQRVYA ALSDSRLLLF DAPDPRGSLA SGVLLQALDL
1010 1020 1030 1040 1050
RDPQFSATPV LAPDVIHAQS KDLPRIFRVT ASQLTVPPTT CTVLLLAENE
1060 1070 1080 1090 1100
GERERWLQVL GELQRLLLDA RPRPRPVYTL KEAYDNGLPL LPHALCAAVI
1110 1120 1130 1140 1150
DQERLALGTE EGLFVIHLHS NDIFQVGDCR RVQRLAVSSA AGLLAVLCGR
1160 1170 1180 1190 1200
GPSVRLFALD ELESAEVAGA KIPESRGCQA LVAGRILQAR TPVLCVAVKR
1210 1220 1230 1240 1250
QVLCYQLGPG PGPWQRRIRE LQAPAPVQSL GLLGDRLCVG AAGTFALYPL
1260 1270 1280 1290 1300
LNEAAPLALG TGLVAEELPA SRGGLGEALG AVELSLSELL LLFATAGVYV
1310 1320 1330 1340 1350
DSAGRKSRSH ELLWPAAPTG WGYTAPYLTV FSENALDVFD VRRAEWVQTV
1360 1370 1380 1390 1400
PLKKVRPLNP EGSLFLYGTE KVRLTYLRNP LAEKDEFDIP DLTDNSRRQL
1410 1420 1430 1440 1450
FRTKSKRRFF FRVSDELRQQ QRREMLKDPF VRSKFISPPT NFNHLVHVGP
1460 1470 1480 1490 1500
TEGRPNTRDG TRAQEQKSRG ARSSGPQRPH SFSEAFRRPV STGSDGLPGE
1510 1520 1530 1540 1550
TDPLVKRKPW TSLSSESVSC PQGSLSPAAS LIQVSERPRS LPPDPESESS

P
Length:1,551
Mass (Da):172,147
Last modified:November 8, 2005 - v2
Checksum:i74458CA5FEF6F8A7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1065 – 10651R → Q in AAH46418 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC127556 Genomic DNA. No translation available.
BC046418 mRNA. Translation: AAH46418.1.
CCDSiCCDS50365.1.
RefSeqiNP_001028514.1. NM_001033342.1.
UniGeneiMm.329622.

Genome annotation databases

EnsembliENSMUST00000025681; ENSMUSP00000025681; ENSMUSG00000024769.
ENSMUST00000201699; ENSMUSP00000144638; ENSMUSG00000107268.
GeneIDi240505.
KEGGimmu:240505.
UCSCiuc008gia.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC127556 Genomic DNA. No translation available.
BC046418 mRNA. Translation: AAH46418.1.
CCDSiCCDS50365.1.
RefSeqiNP_001028514.1. NM_001033342.1.
UniGeneiMm.329622.

3D structure databases

ProteinModelPortaliQ80UW5.
SMRiQ80UW5. Positions 2-400.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi232205. 1 interaction.
IntActiQ80UW5. 1 interaction.
STRINGi10090.ENSMUSP00000025681.

PTM databases

PhosphoSiteiQ80UW5.

Proteomic databases

MaxQBiQ80UW5.
PaxDbiQ80UW5.
PRIDEiQ80UW5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025681; ENSMUSP00000025681; ENSMUSG00000024769.
ENSMUST00000201699; ENSMUSP00000144638; ENSMUSG00000107268.
GeneIDi240505.
KEGGimmu:240505.
UCSCiuc008gia.2. mouse.

Organism-specific databases

CTDi55561.
MGIiMGI:2652845. Cdc42bpg.

Phylogenomic databases

eggNOGiKOG0612. Eukaryota.
ENOG410XR1Q. LUCA.
GeneTreeiENSGT00760000118994.
HOGENOMiHOG000294133.
HOVERGENiHBG055933.
InParanoidiQ80UW5.
KOiK16307.
OMAiFRNHPFF.
OrthoDBiEOG7F511X.
PhylomeDBiQ80UW5.
TreeFamiTF313551.

Miscellaneous databases

ChiTaRSiCdc42bpg. mouse.
NextBioi384604.
PROiQ80UW5.
SOURCEiSearch...

Gene expression databases

BgeeiQ80UW5.
CleanExiMM_CDC42BPG.
GenevisibleiQ80UW5. MM.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR001180. CNH_dom.
IPR000095. CRIB_dom.
IPR011009. Kinase-like_dom.
IPR033235. MRCK_gamma.
IPR014930. Myotonic_dystrophy_kinase_coil.
IPR002219. PE/DAG-bd.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR22988:SF22. PTHR22988:SF22. 1 hit.
PfamiPF00130. C1_1. 1 hit.
PF00780. CNH. 1 hit.
PF08826. DMPK_coil. 1 hit.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
ProDomiPD011252. Myotonic_dystrophy_kinase_coil. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00109. C1. 1 hit.
SM00036. CNH. 1 hit.
SM00285. PBD. 1 hit.
SM00233. PH. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50219. CNH. 1 hit.
PS50108. CRIB. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 699-1551.
    Strain: FVB/NImported.
    Tissue: Mammary glandImported.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney, Lung and Pancreas.

Entry informationi

Entry nameiMRCKG_MOUSE
AccessioniPrimary (citable) accession number: Q80UW5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: May 11, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.