Q80UW0 (H6ST2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heparan-sulfate 6-O-sulfotransferase 2
Short name=HS6ST-2
Short name=mHS6ST-2
EC=2.8.2.-

Gene names

Name:

Hs6st2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	612 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate.
Catalytic activity	3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 6-sulfate.
Subcellular location	Membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the sulfotransferase 6 family.
Sequence caution	The sequence BAC34950.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	sulfotransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q80UW0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q80UW0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-146: Missing.

Isoform 3 (identifier: Q80UW0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     316-316: R → RWRIFQILDGTSKDRWGSSNFNSGANSPSSTKPRSTSKSGK

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 612	612	Heparan-sulfate 6-O-sulfotransferase 2		PRO_0000190806
Regions
	Topological domain	1 – 4	4	Cytoplasmic Potential
	Transmembrane	5 – 27	23	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	28 – 612	585	Lumenal Potential
	Region	236 – 242	7	5'-phosphosulfate-binding Potential
	Region	325 – 333	9	3'-phosphate binding Potential
Amino acid modifications
	Glycosylation	209	1	N-linked (GlcNAc...) Potential
	Glycosylation	404	1	N-linked (GlcNAc...) Potential
	Glycosylation	460	1	N-linked (GlcNAc...) Potential
	Glycosylation	546	1	N-linked (GlcNAc...) Potential
	Glycosylation	558	1	N-linked (GlcNAc...) Potential
	Glycosylation	562	1	N-linked (GlcNAc...) Potential
	Glycosylation	574	1	N-linked (GlcNAc...) Potential
	Glycosylation	599	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 146	146	Missing in isoform 2.		VSP_015848
	Alternative sequence	316	1	R → RWRIFQILDGTSKDRWGSSN FNSGANSPSSTKPRSTSKSG K in isoform 3.		VSP_015849
Experimental info
	Sequence conflict	419	1	E → D in BAE42608. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 9, 2007. Version 3. Checksum: 12551E4408936D8D	FASTA	612	69,198
	10 20 30 40 50 60 MALPAFAARA LGPPLQPEQG APARTTCPRR HSRVEAELAA SRPGSVAASV RAGPPRGVSL 70 80 90 100 110 120 GFNSPPLQDK PPKAFSSLAG ALRAPLFALL PRGRRRRMHD LRRRWDLGSL CRALLTRGLA 130 140 150 160 170 180 AVGHSLKHVL SAIFSKIFGP LASVGNMDEK SNKLLLALVM LFLFAVIVLQ YVCPGTECQL 190 200 210 220 230 240 LRLQAFSSPV PDPYRSEDES SARFVPRYNF SRGDLLRKVD FDIKGDDLIV FLHIQKTGGT 250 260 270 280 290 300 TFGRHLVRNI QLEQPCECRV GQKKCTCHRP GKRETWLFSR FSTGWSCGLH ADWTELTSCV 310 320 330 340 350 360 PAVVDGKRDA RLRPSRNFHY ITILRDPVSR YLSEWRHVQR GATWKASLHV CDGRPPTSEE 370 380 390 400 410 420 LPSCYTGDDW SGCPLKEFMD CPYNLANNRQ VRMLSDLTLV GCYNLSVMPE KQRNKVLLES 430 440 450 460 470 480 AKSNLKHMAF FGLTEFQRKT QYLFEKTFNM NFISPFTQYN TTRASSVEIN EEIQKRIEGL 490 500 510 520 530 540 NFLDMELYSY AKDLFLQRYQ FMRQKEHQDA RRKRQEQRKF LKGRFLQTHF QSQSQGQSQS 550 560 570 580 590 600 QSPGQNLSQN PNPNPNQNLT QNLSHNLTPS SNPNSTQREN RGSQKQGSGQ GQGDSGTSNG 610 TNDYIGSVET WR
	Isoform 2 [UniParc]. Checksum: 12304F0E8EDF93CE	FASTA	466	53,737
	10 20 30 40 50 60 MDEKSNKLLL ALVMLFLFAV IVLQYVCPGT ECQLLRLQAF SSPVPDPYRS EDESSARFVP 70 80 90 100 110 120 RYNFSRGDLL RKVDFDIKGD DLIVFLHIQK TGGTTFGRHL VRNIQLEQPC ECRVGQKKCT 130 140 150 160 170 180 CHRPGKRETW LFSRFSTGWS CGLHADWTEL TSCVPAVVDG KRDARLRPSR NFHYITILRD 190 200 210 220 230 240 PVSRYLSEWR HVQRGATWKA SLHVCDGRPP TSEELPSCYT GDDWSGCPLK EFMDCPYNLA 250 260 270 280 290 300 NNRQVRMLSD LTLVGCYNLS VMPEKQRNKV LLESAKSNLK HMAFFGLTEF QRKTQYLFEK 310 320 330 340 350 360 TFNMNFISPF TQYNTTRASS VEINEEIQKR IEGLNFLDME LYSYAKDLFL QRYQFMRQKE 370 380 390 400 410 420 HQDARRKRQE QRKFLKGRFL QTHFQSQSQG QSQSQSPGQN LSQNPNPNPN QNLTQNLSHN 430 440 450 460 LTPSSNPNST QRENRGSQKQ GSGQGQGDSG TSNGTNDYIG SVETWR
	Isoform 3 [UniParc]. Checksum: 4E3F3D370B4E3FC3	FASTA	652	73,553
	10 20 30 40 50 60 MALPAFAARA LGPPLQPEQG APARTTCPRR HSRVEAELAA SRPGSVAASV RAGPPRGVSL 70 80 90 100 110 120 GFNSPPLQDK PPKAFSSLAG ALRAPLFALL PRGRRRRMHD LRRRWDLGSL CRALLTRGLA 130 140 150 160 170 180 AVGHSLKHVL SAIFSKIFGP LASVGNMDEK SNKLLLALVM LFLFAVIVLQ YVCPGTECQL 190 200 210 220 230 240 LRLQAFSSPV PDPYRSEDES SARFVPRYNF SRGDLLRKVD FDIKGDDLIV FLHIQKTGGT 250 260 270 280 290 300 TFGRHLVRNI QLEQPCECRV GQKKCTCHRP GKRETWLFSR FSTGWSCGLH ADWTELTSCV 310 320 330 340 350 360 PAVVDGKRDA RLRPSRWRIF QILDGTSKDR WGSSNFNSGA NSPSSTKPRS TSKSGKNFHY 370 380 390 400 410 420 ITILRDPVSR YLSEWRHVQR GATWKASLHV CDGRPPTSEE LPSCYTGDDW SGCPLKEFMD 430 440 450 460 470 480 CPYNLANNRQ VRMLSDLTLV GCYNLSVMPE KQRNKVLLES AKSNLKHMAF FGLTEFQRKT 490 500 510 520 530 540 QYLFEKTFNM NFISPFTQYN TTRASSVEIN EEIQKRIEGL NFLDMELYSY AKDLFLQRYQ 550 560 570 580 590 600 FMRQKEHQDA RRKRQEQRKF LKGRFLQTHF QSQSQGQSQS QSPGQNLSQN PNPNPNQNLT 610 620 630 640 650 QNLSHNLTPS SNPNSTQREN RGSQKQGSGQ GQGDSGTSNG TNDYIGSVET WR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 75-409 (ISOFORM 3). Strain: C57BL/6J. Tissue: Heart.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 78-612 (ISOFORM 1). Strain: FVB/N-3. Tissue: Brain and Mammary tumor.
[4]	"The occurrence of three isoforms of heparan sulfate 6-O-sulfotransferase having different specificities for hexuronic acid adjacent to the targeted N-sulfoglucosamine." Habuchi H., Tanaka M., Habuchi O., Yoshida K., Suzuki H., Ban K., Kimata K. J. Biol. Chem. 275:2859-2868(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 147-612 (ISOFORM 3). Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK052348 mRNA. Translation: BAC34950.1. Different initiation. AK171680 mRNA. Translation: BAE42608.1. AL671918, AL672057 Genomic DNA. Translation: CAM16284.1. AL672057, AL671918 Genomic DNA. Translation: CAM21295.1. AL672099 Genomic DNA. No translation available. BC047151 mRNA. Translation: AAH47151.1. BC063327 mRNA. Translation: AAH63327.1. AB024565 mRNA. Translation: BAA89247.1.
IPI	IPI00136281. IPI00330386. IPI00471111.
RefSeq	NP_001070670.1. NM_001077202.1. NP_056634.3. NM_015819.3.
UniGene	Mm.252561.
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q80UW0.
Proteomic databases
PaxDb	Q80UW0.
PRIDE	Q80UW0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000088172; ENSMUSP00000085497; ENSMUSG00000062184. ENSMUST00000114871; ENSMUSP00000110521; ENSMUSG00000062184.
GeneID	50786.
KEGG	mmu:50786.
UCSC	uc009tdw.1. mouse. uc009tdz.1. mouse.
Organism-specific databases
CTD	90161.
MGI	MGI:1354959. Hs6st2.
Phylogenomic databases
eggNOG	NOG302961.
GeneTree	ENSGT00390000013468.
HOGENOM	HOG000007772.
HOVERGEN	HBG083012.
KO	K08102.
OrthoDB	EOG4X6C8G.
Gene expression databases
ArrayExpress	Q80UW0.
Bgee	Q80UW0.
CleanEx	MM_HS6ST2.
Genevestigator	Q80UW0.
GermOnline	ENSMUSG00000062184. Mus musculus.
Family and domain databases
InterPro	IPR010635. Heparan_SO4-6-sulfoTrfase. IPR005331. Sulfotransferase. [Graphical view]
PANTHER	PTHR12812. PTHR12812. 1 hit.
Pfam	PF03567. Sulfotransfer_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	307753.
SOURCE	Search...

Entry information

Entry name

H6ST2_MOUSE

Accession

Primary (citable) accession number: Q80UW0
Secondary accession number(s): A2AEM4 , Q3TAR0, Q6P4N9, Q8C785, Q9QYK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2005
Last sequence update:	January 9, 2007
Last modified:	April 3, 2013
This is version 77 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program