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Q80UW0

- H6ST2_MOUSE

UniProt

Q80UW0 - H6ST2_MOUSE

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Protein

Heparan-sulfate 6-O-sulfotransferase 2

Gene

Hs6st2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate.

Catalytic activityi

3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 6-sulfate.

GO - Molecular functioni

  1. heparan sulfate 6-O-sulfotransferase activity Source: MGI

GO - Biological processi

  1. heparan sulfate proteoglycan biosynthetic process, enzymatic modification Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

ReactomeiREACT_198654. HS-GAG biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Heparan-sulfate 6-O-sulfotransferase 2 (EC:2.8.2.-)
Short name:
HS6ST-2
Short name:
mHS6ST-2
Gene namesi
Name:Hs6st2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:1354959. Hs6st2.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 612612Heparan-sulfate 6-O-sulfotransferase 2PRO_0000190806Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi209 – 2091N-linked (GlcNAc...)Sequence Analysis
Glycosylationi404 – 4041N-linked (GlcNAc...)Sequence Analysis
Glycosylationi460 – 4601N-linked (GlcNAc...)Sequence Analysis
Glycosylationi546 – 5461N-linked (GlcNAc...)Sequence Analysis
Glycosylationi558 – 5581N-linked (GlcNAc...)Sequence Analysis
Glycosylationi562 – 5621N-linked (GlcNAc...)Sequence Analysis
Glycosylationi574 – 5741N-linked (GlcNAc...)Sequence Analysis
Glycosylationi599 – 5991N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ80UW0.
PaxDbiQ80UW0.
PRIDEiQ80UW0.

PTM databases

PhosphoSiteiQ80UW0.

Expressioni

Gene expression databases

BgeeiQ80UW0.
CleanExiMM_HS6ST2.
GenevestigatoriQ80UW0.

Structurei

3D structure databases

ProteinModelPortaliQ80UW0.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 44CytoplasmicSequence Analysis
Topological domaini28 – 612585LumenalSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei5 – 2723Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni236 – 24275'-phosphosulfate-bindingSequence Analysis
Regioni325 – 33393'-phosphate bindingSequence Analysis

Sequence similaritiesi

Belongs to the sulfotransferase 6 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG302961.
GeneTreeiENSGT00390000013468.
HOGENOMiHOG000007772.
HOVERGENiHBG083012.
InParanoidiQ80UW0.
KOiK08102.
OMAiLRPSRWR.
OrthoDBiEOG75MVW3.
TreeFamiTF312835.

Family and domain databases

InterProiIPR010635. Heparan_SO4-6-sulfoTrfase.
IPR027417. P-loop_NTPase.
IPR005331. Sulfotransferase.
[Graphical view]
PANTHERiPTHR12812. PTHR12812. 1 hit.
PfamiPF03567. Sulfotransfer_2. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q80UW0-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALPAFAARA LGPPLQPEQG APARTTCPRR HSRVEAELAA SRPGSVAASV
60 70 80 90 100
RAGPPRGVSL GFNSPPLQDK PPKAFSSLAG ALRAPLFALL PRGRRRRMHD
110 120 130 140 150
LRRRWDLGSL CRALLTRGLA AVGHSLKHVL SAIFSKIFGP LASVGNMDEK
160 170 180 190 200
SNKLLLALVM LFLFAVIVLQ YVCPGTECQL LRLQAFSSPV PDPYRSEDES
210 220 230 240 250
SARFVPRYNF SRGDLLRKVD FDIKGDDLIV FLHIQKTGGT TFGRHLVRNI
260 270 280 290 300
QLEQPCECRV GQKKCTCHRP GKRETWLFSR FSTGWSCGLH ADWTELTSCV
310 320 330 340 350
PAVVDGKRDA RLRPSRNFHY ITILRDPVSR YLSEWRHVQR GATWKASLHV
360 370 380 390 400
CDGRPPTSEE LPSCYTGDDW SGCPLKEFMD CPYNLANNRQ VRMLSDLTLV
410 420 430 440 450
GCYNLSVMPE KQRNKVLLES AKSNLKHMAF FGLTEFQRKT QYLFEKTFNM
460 470 480 490 500
NFISPFTQYN TTRASSVEIN EEIQKRIEGL NFLDMELYSY AKDLFLQRYQ
510 520 530 540 550
FMRQKEHQDA RRKRQEQRKF LKGRFLQTHF QSQSQGQSQS QSPGQNLSQN
560 570 580 590 600
PNPNPNQNLT QNLSHNLTPS SNPNSTQREN RGSQKQGSGQ GQGDSGTSNG
610
TNDYIGSVET WR
Length:612
Mass (Da):69,198
Last modified:January 9, 2007 - v3
Checksum:i12551E4408936D8D
GO
Isoform 2 (identifier: Q80UW0-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-146: Missing.

Show »
Length:466
Mass (Da):53,737
Checksum:i12304F0E8EDF93CE
GO
Isoform 3 (identifier: Q80UW0-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     316-316: R → RWRIFQILDGTSKDRWGSSNFNSGANSPSSTKPRSTSKSGK

Note: No experimental confirmation available.

Show »
Length:652
Mass (Da):73,553
Checksum:i4E3F3D370B4E3FC3
GO

Sequence cautioni

The sequence BAC34950.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti419 – 4191E → D in BAE42608. (PubMed:16141072)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 146146Missing in isoform 2. 2 PublicationsVSP_015848Add
BLAST
Alternative sequencei316 – 3161R → RWRIFQILDGTSKDRWGSSN FNSGANSPSSTKPRSTSKSG K in isoform 3. 2 PublicationsVSP_015849

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK052348 mRNA. Translation: BAC34950.1. Different initiation.
AK171680 mRNA. Translation: BAE42608.1.
AL671918, AL672057 Genomic DNA. Translation: CAM16284.1.
AL672057, AL671918 Genomic DNA. Translation: CAM21295.1.
AL672099 Genomic DNA. No translation available.
BC047151 mRNA. Translation: AAH47151.1.
BC063327 mRNA. Translation: AAH63327.1.
AB024565 mRNA. Translation: BAA89247.1.
CCDSiCCDS40970.1. [Q80UW0-3]
CCDS72379.1. [Q80UW0-1]
RefSeqiNP_001070670.1. NM_001077202.2. [Q80UW0-3]
NP_001277396.1. NM_001290467.1. [Q80UW0-1]
NP_001277397.1. NM_001290468.1.
NP_056634.3. NM_015819.4. [Q80UW0-2]
XP_006541581.1. XM_006541518.1.
XP_006541582.1. XM_006541519.1.
UniGeneiMm.252561.

Genome annotation databases

EnsembliENSMUST00000088172; ENSMUSP00000085497; ENSMUSG00000062184. [Q80UW0-3]
ENSMUST00000114871; ENSMUSP00000110521; ENSMUSG00000062184. [Q80UW0-1]
GeneIDi50786.
KEGGimmu:50786.
UCSCiuc009tdw.1. mouse. [Q80UW0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK052348 mRNA. Translation: BAC34950.1 . Different initiation.
AK171680 mRNA. Translation: BAE42608.1 .
AL671918 , AL672057 Genomic DNA. Translation: CAM16284.1 .
AL672057 , AL671918 Genomic DNA. Translation: CAM21295.1 .
AL672099 Genomic DNA. No translation available.
BC047151 mRNA. Translation: AAH47151.1 .
BC063327 mRNA. Translation: AAH63327.1 .
AB024565 mRNA. Translation: BAA89247.1 .
CCDSi CCDS40970.1. [Q80UW0-3 ]
CCDS72379.1. [Q80UW0-1 ]
RefSeqi NP_001070670.1. NM_001077202.2. [Q80UW0-3 ]
NP_001277396.1. NM_001290467.1. [Q80UW0-1 ]
NP_001277397.1. NM_001290468.1.
NP_056634.3. NM_015819.4. [Q80UW0-2 ]
XP_006541581.1. XM_006541518.1.
XP_006541582.1. XM_006541519.1.
UniGenei Mm.252561.

3D structure databases

ProteinModelPortali Q80UW0.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q80UW0.

Proteomic databases

MaxQBi Q80UW0.
PaxDbi Q80UW0.
PRIDEi Q80UW0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000088172 ; ENSMUSP00000085497 ; ENSMUSG00000062184 . [Q80UW0-3 ]
ENSMUST00000114871 ; ENSMUSP00000110521 ; ENSMUSG00000062184 . [Q80UW0-1 ]
GeneIDi 50786.
KEGGi mmu:50786.
UCSCi uc009tdw.1. mouse. [Q80UW0-1 ]

Organism-specific databases

CTDi 90161.
MGIi MGI:1354959. Hs6st2.

Phylogenomic databases

eggNOGi NOG302961.
GeneTreei ENSGT00390000013468.
HOGENOMi HOG000007772.
HOVERGENi HBG083012.
InParanoidi Q80UW0.
KOi K08102.
OMAi LRPSRWR.
OrthoDBi EOG75MVW3.
TreeFami TF312835.

Enzyme and pathway databases

Reactomei REACT_198654. HS-GAG biosynthesis.

Miscellaneous databases

NextBioi 307753.
PROi Q80UW0.
SOURCEi Search...

Gene expression databases

Bgeei Q80UW0.
CleanExi MM_HS6ST2.
Genevestigatori Q80UW0.

Family and domain databases

InterProi IPR010635. Heparan_SO4-6-sulfoTrfase.
IPR027417. P-loop_NTPase.
IPR005331. Sulfotransferase.
[Graphical view ]
PANTHERi PTHR12812. PTHR12812. 1 hit.
Pfami PF03567. Sulfotransfer_2. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 75-409 (ISOFORM 3).
    Strain: C57BL/6J.
    Tissue: Heart.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 78-612 (ISOFORM 1).
    Strain: FVB/N-3.
    Tissue: Brain and Mammary tumor.
  4. "The occurrence of three isoforms of heparan sulfate 6-O-sulfotransferase having different specificities for hexuronic acid adjacent to the targeted N-sulfoglucosamine."
    Habuchi H., Tanaka M., Habuchi O., Yoshida K., Suzuki H., Ban K., Kimata K.
    J. Biol. Chem. 275:2859-2868(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 147-612 (ISOFORM 3).
    Tissue: Brain.

Entry informationi

Entry nameiH6ST2_MOUSE
AccessioniPrimary (citable) accession number: Q80UW0
Secondary accession number(s): A2AEM4
, Q3TAR0, Q6P4N9, Q8C785, Q9QYK6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: January 9, 2007
Last modified: October 29, 2014
This is version 92 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3