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Q80US8

- MAD3_MOUSE

UniProt

Q80US8 - MAD3_MOUSE

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Protein

Max dimerization protein 3

Gene

Mxd3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation.1 Publication

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. negative regulation of transcription, DNA-templated Source: MGI
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Max dimerization protein 3
Short name:
Max dimerizer 3
Alternative name(s):
Max-associated protein 3
Gene namesi
Name:Mxd3
Synonyms:Mad3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 13

Organism-specific databases

MGIiMGI:104987. Mxd3.

Subcellular locationi

Nucleus 1 PublicationPROSITE-ProRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 206206Max dimerization protein 3PRO_0000253708Add
BLAST

Proteomic databases

PRIDEiQ80US8.

PTM databases

PhosphoSiteiQ80US8.

Expressioni

Tissue specificityi

Expressed only in the proliferating areas of the testis and thymus.1 Publication

Developmental stagei

Expressed during neural and epidermal differentiation. Expression restricted to proliferating cells prior to differentiation. Specifically expressed in the S phase of the cell cycle in neuronal progenitor cells. In the developing embryo, detected from 9.5 to 12.5 dpc especially in the ventricular zone of the neuroepithelia, in the progression zone of the limb buds and in the aortic arches and liver. In the spinal cord at embryonic days 10.5, 11.5 and 12.5 dpc, expressed in the cells at the perimeter of the ventricular zone. In the developing epidermis, expressed only in the uppermost differentiated cell layers underneath the stratum corneum.2 Publications

Gene expression databases

BgeeiQ80US8.
CleanExiMM_MXD3.
GenevestigatoriQ80US8.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with SIN3A AND SIN3B. Interacts with RNF17.2 Publications

Protein-protein interaction databases

BioGridi201271. 1 interaction.
DIPiDIP-948N.

Structurei

3D structure databases

ProteinModelPortaliQ80US8.
SMRiQ80US8. Positions 59-136.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini57 – 10953bHLHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni8 – 2518Interaction with SIN3A and SIN3BAdd
BLAST

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG247787.
GeneTreeiENSGT00510000046360.
HOGENOMiHOG000247060.
HOVERGENiHBG006314.
InParanoidiQ80US8.
KOiK09114.
OMAiEHSYSHS.
OrthoDBiEOG76T9V0.
PhylomeDBiQ80US8.
TreeFamiTF315654.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q80US8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEPVASNIQV LLQAAEFLER REREAEHGYA SLCPHHSPGT VCRRRKPPLQ
60 70 80 90 100
APGALNSGRS VHNELEKRRR AQLKRCLEQL RQQMPLGVDC TRYTTLSLLR
110 120 130 140 150
RARVHIQKLE EQEQQARRLK EKLRSKQQSL QQQLEQLQGL PGARERERLR
160 170 180 190 200
ADSLDSSGLS SERSDSDQED LEVDVESLVF GTETELLQSF SAGREHSYSH

STCAWL
Length:206
Mass (Da):23,635
Last modified:June 1, 2003 - v1
Checksum:i17E1149064907085
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti177 – 1771S → N in AAB02794. (PubMed:8521822)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U32394 mRNA. Translation: AAB02794.1.
AK138742 mRNA. Translation: BAE23764.1.
BC051970 mRNA. Translation: AAH51970.1.
CCDSiCCDS26543.1.
PIRiS60005.
RefSeqiNP_057871.2. NM_016662.4.
UniGeneiMm.20350.

Genome annotation databases

EnsembliENSMUST00000021941; ENSMUSP00000021941; ENSMUSG00000021485.
GeneIDi17121.
KEGGimmu:17121.
UCSCiuc007qqn.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U32394 mRNA. Translation: AAB02794.1 .
AK138742 mRNA. Translation: BAE23764.1 .
BC051970 mRNA. Translation: AAH51970.1 .
CCDSi CCDS26543.1.
PIRi S60005.
RefSeqi NP_057871.2. NM_016662.4.
UniGenei Mm.20350.

3D structure databases

ProteinModelPortali Q80US8.
SMRi Q80US8. Positions 59-136.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 201271. 1 interaction.
DIPi DIP-948N.

PTM databases

PhosphoSitei Q80US8.

Proteomic databases

PRIDEi Q80US8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000021941 ; ENSMUSP00000021941 ; ENSMUSG00000021485 .
GeneIDi 17121.
KEGGi mmu:17121.
UCSCi uc007qqn.1. mouse.

Organism-specific databases

CTDi 83463.
MGIi MGI:104987. Mxd3.

Phylogenomic databases

eggNOGi NOG247787.
GeneTreei ENSGT00510000046360.
HOGENOMi HOG000247060.
HOVERGENi HBG006314.
InParanoidi Q80US8.
KOi K09114.
OMAi EHSYSHS.
OrthoDBi EOG76T9V0.
PhylomeDBi Q80US8.
TreeFami TF315654.

Miscellaneous databases

NextBioi 291292.
PROi Q80US8.
SOURCEi Search...

Gene expression databases

Bgeei Q80US8.
CleanExi MM_MXD3.
Genevestigatori Q80US8.

Family and domain databases

Gene3Di 4.10.280.10. 1 hit.
InterProi IPR011598. bHLH_dom.
[Graphical view ]
Pfami PF00010. HLH. 1 hit.
[Graphical view ]
SMARTi SM00353. HLH. 1 hit.
[Graphical view ]
SUPFAMi SSF47459. SSF47459. 1 hit.
PROSITEi PS50888. BHLH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mad3 and Mad4: novel Max-interacting transcriptional repressors that suppress c-myc dependent transformation and are expressed during neural and epidermal differentiation."
    Hurlin P.J., Queva C., Koskinen P.J., Steingrimsson E., Ayer D.E., Copeland N.G., Jenkins N.A., Eisenman R.N.
    EMBO J. 14:5646-5659(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, SUBUNIT, SUBCELLULAR LOCATION, INTERACTION WITH SIN3A AND SIN3B.
    Strain: AB1.
    Tissue: Embryonic stem cell.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
  4. "Targeted deletion of the S-phase-specific Myc antagonist Mad3 sensitizes neuronal and lymphoid cells to radiation-induced apoptosis."
    Queva C., McArthur G.A., Iritani B.M., Eisenman R.N.
    Mol. Cell. Biol. 21:703-712(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  5. "Mmip-2, a novel RING finger protein that interacts with mad members of the Myc oncoprotein network."
    Yin X.-Y., Gupta K., Prochownik E.V.
    Oncogene 18:6621-6634(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RNF17.

Entry informationi

Entry nameiMAD3_MOUSE
AccessioniPrimary (citable) accession number: Q80US8
Secondary accession number(s): Q60947
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: June 1, 2003
Last modified: October 29, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Mice deficient for Mxd3 show increased sensitivity of neuronal and lymphoid cells to gamma-radiation induced apoptosis.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3