Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q80US8

- MAD3_MOUSE

UniProt

Q80US8 - MAD3_MOUSE

Protein

Max dimerization protein 3

Gene

Mxd3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation.1 Publication

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. protein binding Source: MGI

    GO - Biological processi

    1. negative regulation of transcription, DNA-templated Source: MGI
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Max dimerization protein 3
    Short name:
    Max dimerizer 3
    Alternative name(s):
    Max-associated protein 3
    Gene namesi
    Name:Mxd3
    Synonyms:Mad3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:104987. Mxd3.

    Subcellular locationi

    Nucleus 1 PublicationPROSITE-ProRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 206206Max dimerization protein 3PRO_0000253708Add
    BLAST

    Proteomic databases

    PRIDEiQ80US8.

    PTM databases

    PhosphoSiteiQ80US8.

    Expressioni

    Tissue specificityi

    Expressed only in the proliferating areas of the testis and thymus.1 Publication

    Developmental stagei

    Expressed during neural and epidermal differentiation. Expression restricted to proliferating cells prior to differentiation. Specifically expressed in the S phase of the cell cycle in neuronal progenitor cells. In the developing embryo, detected from 9.5 to 12.5 dpc especially in the ventricular zone of the neuroepithelia, in the progression zone of the limb buds and in the aortic arches and liver. In the spinal cord at embryonic days 10.5, 11.5 and 12.5 dpc, expressed in the cells at the perimeter of the ventricular zone. In the developing epidermis, expressed only in the uppermost differentiated cell layers underneath the stratum corneum.2 Publications

    Gene expression databases

    BgeeiQ80US8.
    CleanExiMM_MXD3.
    GenevestigatoriQ80US8.

    Interactioni

    Subunit structurei

    Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with SIN3A AND SIN3B. Interacts with RNF17.2 Publications

    Protein-protein interaction databases

    BioGridi201271. 1 interaction.
    DIPiDIP-948N.

    Structurei

    3D structure databases

    ProteinModelPortaliQ80US8.
    SMRiQ80US8. Positions 59-136.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini57 – 10953bHLHPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni8 – 2518Interaction with SIN3A and SIN3BAdd
    BLAST

    Sequence similaritiesi

    Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG247787.
    GeneTreeiENSGT00510000046360.
    HOGENOMiHOG000247060.
    HOVERGENiHBG006314.
    InParanoidiQ80US8.
    KOiK09114.
    OMAiEHSYSHS.
    OrthoDBiEOG76T9V0.
    PhylomeDBiQ80US8.
    TreeFamiTF315654.

    Family and domain databases

    Gene3Di4.10.280.10. 1 hit.
    InterProiIPR011598. bHLH_dom.
    [Graphical view]
    PfamiPF00010. HLH. 1 hit.
    [Graphical view]
    SMARTiSM00353. HLH. 1 hit.
    [Graphical view]
    SUPFAMiSSF47459. SSF47459. 1 hit.
    PROSITEiPS50888. BHLH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q80US8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEPVASNIQV LLQAAEFLER REREAEHGYA SLCPHHSPGT VCRRRKPPLQ    50
    APGALNSGRS VHNELEKRRR AQLKRCLEQL RQQMPLGVDC TRYTTLSLLR 100
    RARVHIQKLE EQEQQARRLK EKLRSKQQSL QQQLEQLQGL PGARERERLR 150
    ADSLDSSGLS SERSDSDQED LEVDVESLVF GTETELLQSF SAGREHSYSH 200
    STCAWL 206
    Length:206
    Mass (Da):23,635
    Last modified:June 1, 2003 - v1
    Checksum:i17E1149064907085
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti177 – 1771S → N in AAB02794. (PubMed:8521822)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U32394 mRNA. Translation: AAB02794.1.
    AK138742 mRNA. Translation: BAE23764.1.
    BC051970 mRNA. Translation: AAH51970.1.
    CCDSiCCDS26543.1.
    PIRiS60005.
    RefSeqiNP_057871.2. NM_016662.4.
    UniGeneiMm.20350.

    Genome annotation databases

    EnsembliENSMUST00000021941; ENSMUSP00000021941; ENSMUSG00000021485.
    GeneIDi17121.
    KEGGimmu:17121.
    UCSCiuc007qqn.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U32394 mRNA. Translation: AAB02794.1 .
    AK138742 mRNA. Translation: BAE23764.1 .
    BC051970 mRNA. Translation: AAH51970.1 .
    CCDSi CCDS26543.1.
    PIRi S60005.
    RefSeqi NP_057871.2. NM_016662.4.
    UniGenei Mm.20350.

    3D structure databases

    ProteinModelPortali Q80US8.
    SMRi Q80US8. Positions 59-136.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201271. 1 interaction.
    DIPi DIP-948N.

    PTM databases

    PhosphoSitei Q80US8.

    Proteomic databases

    PRIDEi Q80US8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000021941 ; ENSMUSP00000021941 ; ENSMUSG00000021485 .
    GeneIDi 17121.
    KEGGi mmu:17121.
    UCSCi uc007qqn.1. mouse.

    Organism-specific databases

    CTDi 83463.
    MGIi MGI:104987. Mxd3.

    Phylogenomic databases

    eggNOGi NOG247787.
    GeneTreei ENSGT00510000046360.
    HOGENOMi HOG000247060.
    HOVERGENi HBG006314.
    InParanoidi Q80US8.
    KOi K09114.
    OMAi EHSYSHS.
    OrthoDBi EOG76T9V0.
    PhylomeDBi Q80US8.
    TreeFami TF315654.

    Miscellaneous databases

    NextBioi 291292.
    PROi Q80US8.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q80US8.
    CleanExi MM_MXD3.
    Genevestigatori Q80US8.

    Family and domain databases

    Gene3Di 4.10.280.10. 1 hit.
    InterProi IPR011598. bHLH_dom.
    [Graphical view ]
    Pfami PF00010. HLH. 1 hit.
    [Graphical view ]
    SMARTi SM00353. HLH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47459. SSF47459. 1 hit.
    PROSITEi PS50888. BHLH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mad3 and Mad4: novel Max-interacting transcriptional repressors that suppress c-myc dependent transformation and are expressed during neural and epidermal differentiation."
      Hurlin P.J., Queva C., Koskinen P.J., Steingrimsson E., Ayer D.E., Copeland N.G., Jenkins N.A., Eisenman R.N.
      EMBO J. 14:5646-5659(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, SUBUNIT, SUBCELLULAR LOCATION, INTERACTION WITH SIN3A AND SIN3B.
      Strain: AB1.
      Tissue: Embryonic stem cell.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Thymus.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
    4. "Targeted deletion of the S-phase-specific Myc antagonist Mad3 sensitizes neuronal and lymphoid cells to radiation-induced apoptosis."
      Queva C., McArthur G.A., Iritani B.M., Eisenman R.N.
      Mol. Cell. Biol. 21:703-712(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    5. "Mmip-2, a novel RING finger protein that interacts with mad members of the Myc oncoprotein network."
      Yin X.-Y., Gupta K., Prochownik E.V.
      Oncogene 18:6621-6634(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RNF17.

    Entry informationi

    Entry nameiMAD3_MOUSE
    AccessioniPrimary (citable) accession number: Q80US8
    Secondary accession number(s): Q60947
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 17, 2006
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Mice deficient for Mxd3 show increased sensitivity of neuronal and lymphoid cells to gamma-radiation induced apoptosis.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3