Reviewed, UniProtKB/Swiss-Prot Q80US8 (MAD3_MOUSE)

Names and origin

Protein names

Recommended name:
    Max-interacting transcriptional repressor MAD3
Alternative name(s):
    Max-associated protein 3
    MAX dimerization protein 3

Gene names

Name:	Mxd3
Synonyms:	Mad3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	206 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation. Ref.1
Subunit structure	Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with SIN3A AND SIN3B. Interacts with RNF17. Ref.1 Ref.6
Subcellular location	Nucleus. Ref.1
Tissue specificity	Expressed only in the proliferating areas of the testis and thymus. Ref.5
Developmental stage	Expressed during neural and epidermal differentiation. Expression restricted to proliferating cells prior to differentiation. Specifically expressed in the S phase of the cell cycle in neuronal progenitor cells. In the developing embryo, detected from 9.5 to 12.5 dpc especially in the ventricular zone of the neuroepithelia, in the progression zone of the limb buds and in the aortic arches and liver. In the spinal cord at embryonic days 10.5, 11.5 and 12.5 dpc, expressed in the cells at the perimeter of the ventricular zone. In the developing epidermis, expressed only in the uppermost differentiated cell layers underneath the stratum corneum. Ref.1 Ref.5
Miscellaneous	Mice deficient for Mxd3 show increased sensitivity of neuronal and lymphoid cells to gamma-radiation induced apoptosis.
Sequence similarities	Contains 1 basic helix-loop-helix (bHLH) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Repressor
Gene Ontology (GO)
Biological process	negative regulation of transcription Ref.1 Inferred from genetic interaction. Source: MGI regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Ref.1 Inferred from electronic annotation. Source: UniProtKB-KW protein binding Ref.1 Inferred from physical interaction. Source: MGI transcription regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 206	206	Max-interacting transcriptional repressor MAD3		PRO_0000253708
Regions
	Domain	71 – 110	40	Helix-loop-helix motif
	DNA binding	59 – 70	12	Basic motif
	Region	8 – 25	18	Interaction with SIN3A and SIN3B
Experimental info
	Sequence conflict	177	1	S → N in AAB02794. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q80US8-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 17E1149064907085	FASTA	206	23,635
	10 20 30 40 50 60 MEPVASNIQV LLQAAEFLER REREAEHGYA SLCPHHSPGT VCRRRKPPLQ APGALNSGRS 70 80 90 100 110 120 VHNELEKRRR AQLKRCLEQL RQQMPLGVDC TRYTTLSLLR RARVHIQKLE EQEQQARRLK 130 140 150 160 170 180 EKLRSKQQSL QQQLEQLQGL PGARERERLR ADSLDSSGLS SERSDSDQED LEVDVESLVF 190 200 GTETELLQSF SAGREHSYSH STCAWL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mad3 and Mad4: novel Max-interacting transcriptional repressors that suppress c-myc dependent transformation and are expressed during neural and epidermal differentiation." Hurlin P.J., Queva C., Koskinen P.J., Steingrimsson E., Ayer D.E., Copeland N.G., Jenkins N.A., Eisenman R.N. EMBO J. 14:5646-5659(1995) [PubMed: 8521822] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, SUBUNIT, SUBCELLULAR LOCATION, INTERACTION WITH SIN3A AND SIN3B. Strain: AB1. Tissue: Embryonic stem cell.
[2]	Erratum Hurlin P.J., Queva C., Koskinen P.J., Steingrimsson E., Ayer D.E., Copeland N.G., Jenkins N.A., Eisenman R.N. EMBO J. 15:2030-2030(1996) [PubMed: 8617250] [Abstract]
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Thymus.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6.
[5]	"Targeted deletion of the S-phase-specific Myc antagonist Mad3 sensitizes neuronal and lymphoid cells to radiation-induced apoptosis." Queva C., McArthur G.A., Iritani B.M., Eisenman R.N. Mol. Cell. Biol. 21:703-712(2001) [PubMed: 11154258] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[6]	"Mmip-2, a novel RING finger protein that interacts with mad members of the Myc oncoprotein network." Yin X.-Y., Gupta K., Prochownik E.V. Oncogene 18:6621-6634(1999) [PubMed: 10597267] [Abstract] Cited for: INTERACTION WITH RNF17.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U32394 mRNA. Translation: AAB02794.1. AK138742 mRNA. Translation: BAE23764.1. BC051970 mRNA. Translation: AAH51970.1.
IPI	IPI00122588.
PIR	S60005.
RefSeq	NP_057871.2.
UniGene	Mm.20350
3D structure databases
HSSP	HSSP built from PDB template 1PD7 based on UniProtKB Q05195.
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000021485. Mus musculus. [Contig view]
GeneID	17121.
KEGG	mmu:17121.
Organism-specific databases
MGI	MGI:104987. Mxd3.
Phylogenomic databases
HOVERGEN	Q80US8.
OMA	Q80US8. QEHSYSH.
Gene expression databases
ArrayExpress	Q80US8.
Bgee	Q80US8.
CleanEx	MM_MXD3.
GermOnline	ENSMUSG00000021485. Mus musculus.
Family and domain databases
InterPro	IPR001092. HLH_basic. IPR011598. HLH_DNA_bd. [Graphical view]
Gene3D	G3DSA:4.10.280.10. HLH_DNA_bd. 1 hit.
Pfam	PF00010. HLH. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. [Graphical view]
PROSITE	PS50888. HLH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	291292.
SOURCE	Search...

Entry information

Entry name

MAD3_MOUSE

Accession

Primary (citable) accession number: Q80US8
Secondary accession number(s): Q60947

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)