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Q80UP3

- DGKZ_MOUSE

UniProt

Q80UP3 - DGKZ_MOUSE

Protein

Diacylglycerol kinase zeta

Gene

Dgkz

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Displays a strong preference for 1,2-diacylglycerols over 1,3-diacylglycerols, but lacks substrate specificity among molecular species of long chain diacylglycerols. Regulates RASGRP1 activity By similarity.By similarity

    Catalytic activityi

    ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri98 – 15356Phorbol-ester/DAG-type 1Add
    BLAST
    Zinc fingeri173 – 23159Phorbol-ester/DAG-type 2Add
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. diacylglycerol kinase activity Source: UniProtKB-EC
    3. enzyme inhibitor activity Source: MGI
    4. metal ion binding Source: UniProtKB-KW
    5. NAD+ kinase activity Source: InterPro
    6. protein binding Source: IntAct

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro
    2. negative regulation of catalytic activity Source: GOC
    3. negative regulation of Ras protein signal transduction Source: MGI
    4. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_219232. Effects of PIP2 hydrolysis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diacylglycerol kinase zeta (EC:2.7.1.107)
    Short name:
    DAG kinase zeta
    Alternative name(s):
    Diglyceride kinase zeta
    Short name:
    DGK-zeta
    Gene namesi
    Name:Dgkz
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:1278339. Dgkz.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication. Cell membrane By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 929929Diacylglycerol kinase zetaPRO_0000218469Add
    BLAST

    Proteomic databases

    PaxDbiQ80UP3.
    PRIDEiQ80UP3.

    PTM databases

    PhosphoSiteiQ80UP3.

    Expressioni

    Gene expression databases

    ArrayExpressiQ80UP3.
    BgeeiQ80UP3.
    CleanExiMM_DGKZ.
    GenevestigatoriQ80UP3.

    Interactioni

    Subunit structurei

    Interacts with the PDZ domain of the syntrophin SNTG1 and that of SNX27. Forms a signaling complex with RASGRP1 and HRAS By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Rb1P134052EBI-971774,EBI-971782

    Protein-protein interaction databases

    BioGridi222626. 8 interactions.
    IntActiQ80UP3. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ80UP3.
    SMRiQ80UP3. Positions 792-927.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini292 – 426135DAGKcPROSITE-ProRule annotationAdd
    BLAST
    Repeati823 – 85331ANK 1Add
    BLAST
    Repeati858 – 88730ANK 2Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni279 – 417139Mediates interaction with RASGRP1By similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi362 – 3709Nuclear export signal

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi67 – 726Poly-Pro
    Compositional biasi260 – 2634Poly-Lys
    Compositional biasi372 – 3754Poly-Pro

    Sequence similaritiesi

    Contains 2 ANK repeats.PROSITE-ProRule annotation
    Contains 1 DAGKc domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri98 – 15356Phorbol-ester/DAG-type 1Add
    BLAST
    Zinc fingeri173 – 23159Phorbol-ester/DAG-type 2Add
    BLAST

    Keywords - Domaini

    ANK repeat, Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG47311.
    GeneTreeiENSGT00740000115137.
    HOGENOMiHOG000231472.
    HOVERGENiHBG067303.
    KOiK00901.

    Family and domain databases

    Gene3Di1.25.40.20. 1 hit.
    InterProiIPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view]
    PfamiPF12796. Ank_2. 1 hit.
    PF00130. C1_1. 1 hit.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    [Graphical view]
    SMARTiSM00248. ANK. 2 hits.
    SM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 1 hit.
    SSF48403. SSF48403. 1 hit.
    PROSITEiPS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 2 hits.
    PS50146. DAGK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q80UP3-1 [UniParc]FASTAAdd to Basket

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    MEPRDPSPEG RSSDSESASA SSSGSERDAG PEPDKAPRRL TKRRFPGLRL    50
    FGHRKAITKS GLQHLAPPPP TPGAPCGESE EQIQSTVDWS ESAVYGEHIW 100
    FETNVSGDFC YVGEQHCVAK MLPKSAPRKK CAACKIVVHT QCIKQLEKIN 150
    FRCKPSFRES GSRNVREPTF VRHHWVHRRR QDGKCRHCGK GFQQKFTFHS 200
    KEIVAISCSW CKQAYHSKVS CFMMQQIEEP CSLGVHAAVV IPPTWILRAR 250
    RPQNTLKASK KKKRASFKRR SSKKGPEEGR WRPFIIRPTP SPLMKPLLVF 300
    VNPKSGGNQG AKIIQSFLWY LNPRQVFDLS QGGPREALEM YRKVHNLRIL 350
    ACGGDGTVGW ILSTLDQLRL KPPPPVAILP LGTGNDLART LNWGGGYTDE 400
    PVSKILSHVE EGNVVQLDRW DLRAEPNPEA GPEERDDGAT DRLPLDVFNN 450
    YFSLGFDAHV TLEFHESREA NPEKFNSRFR NKMFYAGTAF SDFLMGSSKD 500
    LAKHIRVVCD GMDLTPKIQD LKPQCIVFLN IPRYCAGTMP WGHPGEHHDF 550
    EPQRHDDGYL EVIGFTMTSL AALQVGGHGE RLTQCREVLL TTAKAIPVQV 600
    DGEPCKLSAS RIRIALRNQA TMVQKAKRRS TAPLHSDQQP VPEQLRIQVS 650
    RVSMHDYEAL HYDKEQLKEA SVPLGTVVVP GDSDLELCRA HIERLQREPD 700
    GAGAKSPMCH QLSSKWCFLD ATTASRFYRI DRAQEHLNYV TEIAQDEIYI 750
    LDPELLGASA RPDLPTPTSP LPASPCSPTP GSMQGDTALP QGEELIEAAK 800
    RNDCCKLQEL HRAGGDLMHR DQKSRTLLHH AVSTGSKEVV RYLLDHAPPE 850
    ILDAVEENGE TCLHQAAALG QRTICHYIVE AGASLMKTDL QGDTPRQRAE 900
    KAQDTELAAY LENRQHYQMI QREDQETAV 929
    Length:929
    Mass (Da):104,031
    Last modified:July 27, 2011 - v2
    Checksum:iAC3E48790E3C3463
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti908 – 9081A → S in AAH49228. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL731772, AL732484 Genomic DNA. Translation: CAM19275.1.
    AL732484, AL731772 Genomic DNA. Translation: CAM22583.1.
    BC049228 mRNA. Translation: AAH49228.1.
    CCDSiCCDS16441.1.
    RefSeqiNP_612179.2. NM_138306.2.
    UniGeneiMm.314923.

    Genome annotation databases

    EnsembliENSMUST00000028667; ENSMUSP00000028667; ENSMUSG00000040479.
    GeneIDi104418.
    KEGGimmu:104418.
    UCSCiuc008kxc.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL731772 , AL732484 Genomic DNA. Translation: CAM19275.1 .
    AL732484 , AL731772 Genomic DNA. Translation: CAM22583.1 .
    BC049228 mRNA. Translation: AAH49228.1 .
    CCDSi CCDS16441.1.
    RefSeqi NP_612179.2. NM_138306.2.
    UniGenei Mm.314923.

    3D structure databases

    ProteinModelPortali Q80UP3.
    SMRi Q80UP3. Positions 792-927.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 222626. 8 interactions.
    IntActi Q80UP3. 1 interaction.

    PTM databases

    PhosphoSitei Q80UP3.

    Proteomic databases

    PaxDbi Q80UP3.
    PRIDEi Q80UP3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000028667 ; ENSMUSP00000028667 ; ENSMUSG00000040479 .
    GeneIDi 104418.
    KEGGi mmu:104418.
    UCSCi uc008kxc.2. mouse.

    Organism-specific databases

    CTDi 8525.
    MGIi MGI:1278339. Dgkz.

    Phylogenomic databases

    eggNOGi NOG47311.
    GeneTreei ENSGT00740000115137.
    HOGENOMi HOG000231472.
    HOVERGENi HBG067303.
    KOi K00901.

    Enzyme and pathway databases

    Reactomei REACT_219232. Effects of PIP2 hydrolysis.

    Miscellaneous databases

    ChiTaRSi DGKZ. mouse.
    NextBioi 357101.
    PROi Q80UP3.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q80UP3.
    Bgeei Q80UP3.
    CleanExi MM_DGKZ.
    Genevestigatori Q80UP3.

    Family and domain databases

    Gene3Di 1.25.40.20. 1 hit.
    InterProi IPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view ]
    Pfami PF12796. Ank_2. 1 hit.
    PF00130. C1_1. 1 hit.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    [Graphical view ]
    SMARTi SM00248. ANK. 2 hits.
    SM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 1 hit.
    SSF48403. SSF48403. 1 hit.
    PROSITEi PS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 2 hits.
    PS50146. DAGK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Mammary tumor.
    3. "Identification of a functional nuclear export sequence in diacylglycerol kinase-zeta."
      Evangelisti C., Gaboardi G.C., Billi A.M., Ognibene A., Goto K., Tazzari P.L., McCubrey J.A., Martelli A.M.
      Cell Cycle 9:384-388(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, NUCLEAR EXPORT SIGNAL.

    Entry informationi

    Entry nameiDGKZ_MOUSE
    AccessioniPrimary (citable) accession number: Q80UP3
    Secondary accession number(s): A2AHJ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 22, 2003
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3