Reviewed, UniProtKB/Swiss-Prot Q80UP3 (DGKZ_MOUSE)

Names and origin

Protein names

Recommended name:
    Diacylglycerol kinase zeta
      Short name=DAG kinase zeta
    EC=2.7.1.107
Alternative name(s):
    Diglyceride kinase zeta
      Short name=DGK-zeta

Gene names

Name:

Dgkz

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	929 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Displays a strong preference for 1,2-diacylglycerols over 1,3-diacylglycerols, but lacks substrate specificity among molecular species of long chain diacylglycerols. Regulates RASGRP1 activity By similarity.
Catalytic activity	ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.
Subunit structure	Interacts with the PDZ domain of the syntrophin SNTG1 and that of SNX27. Forms a signaling complex with RASGRP1 and HRAS By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Cell membrane By similarity.
Sequence similarities	Belongs to the eukaryotic diacylglycerol kinase family. Contains 2 ANK repeats. Contains 1 DAGKc domain. Contains 2 phorbol-ester/DAG-type zinc fingers.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Membrane Nucleus
Domain	ANK repeat Phorbol-ester binding Repeat Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: InterPro intracellular signaling cascade Inferred from electronic annotation. Source: InterPro negative regulation of Ras protein signal transduction Inferred from mutant phenotype. Source: MGI
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol kinase activity Inferred from electronic annotation. Source: EC enzyme inhibitor activity Inferred from direct assay. Source: MGI protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Rb1	P13405	1	EBI-971774,EBI-971782

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 929	929	Diacylglycerol kinase zeta		PRO_0000218469
Regions
	Domain	292 – 426	135	DAGKc
	Repeat	823 – 853	31	ANK 1
	Repeat	858 – 887	30	ANK 2
	Zinc finger	98 – 153	56	Phorbol-ester/DAG-type 1
	Zinc finger	173 – 231	59	Phorbol-ester/DAG-type 2
	Region	279 – 417	139	Mediates interaction with RASGRP1 By similarity
	Compositional bias	67 – 72	6	Poly-Pro
	Compositional bias	260 – 263	4	Poly-Lys
	Compositional bias	372 – 375	4	Poly-Pro
Amino acid modifications
	Modified residue	498	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q80UP3-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: AC3E48790E223583	FASTA	929	104,047
	10 20 30 40 50 60 MEPRDPSPEG RSSDSESASA SSSGSERDAG PEPDKAPRRL TKRRFPGLRL FGHRKAITKS 70 80 90 100 110 120 GLQHLAPPPP TPGAPCGESE EQIQSTVDWS ESAVYGEHIW FETNVSGDFC YVGEQHCVAK 130 140 150 160 170 180 MLPKSAPRKK CAACKIVVHT QCIKQLEKIN FRCKPSFRES GSRNVREPTF VRHHWVHRRR 190 200 210 220 230 240 QDGKCRHCGK GFQQKFTFHS KEIVAISCSW CKQAYHSKVS CFMMQQIEEP CSLGVHAAVV 250 260 270 280 290 300 IPPTWILRAR RPQNTLKASK KKKRASFKRR SSKKGPEEGR WRPFIIRPTP SPLMKPLLVF 310 320 330 340 350 360 VNPKSGGNQG AKIIQSFLWY LNPRQVFDLS QGGPREALEM YRKVHNLRIL ACGGDGTVGW 370 380 390 400 410 420 ILSTLDQLRL KPPPPVAILP LGTGNDLART LNWGGGYTDE PVSKILSHVE EGNVVQLDRW 430 440 450 460 470 480 DLRAEPNPEA GPEERDDGAT DRLPLDVFNN YFSLGFDAHV TLEFHESREA NPEKFNSRFR 490 500 510 520 530 540 NKMFYAGTAF SDFLMGSSKD LAKHIRVVCD GMDLTPKIQD LKPQCIVFLN IPRYCAGTMP 550 560 570 580 590 600 WGHPGEHHDF EPQRHDDGYL EVIGFTMTSL AALQVGGHGE RLTQCREVLL TTAKAIPVQV 610 620 630 640 650 660 DGEPCKLSAS RIRIALRNQA TMVQKAKRRS TAPLHSDQQP VPEQLRIQVS RVSMHDYEAL 670 680 690 700 710 720 HYDKEQLKEA SVPLGTVVVP GDSDLELCRA HIERLQREPD GAGAKSPMCH QLSSKWCFLD 730 740 750 760 770 780 ATTASRFYRI DRAQEHLNYV TEIAQDEIYI LDPELLGASA RPDLPTPTSP LPASPCSPTP 790 800 810 820 830 840 GSMQGDTALP QGEELIEAAK RNDCCKLQEL HRAGGDLMHR DQKSRTLLHH AVSTGSKEVV 850 860 870 880 890 900 RYLLDHAPPE ILDAVEENGE TCLHQAAALG QRTICHYIVE AGASLMKTDL QGDTPRQRAE 910 920 KAQDTELSAY LENRQHYQMI QREDQETAV

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC049228 mRNA. Translation: AAH49228.1.
IPI	IPI00330332.
RefSeq	NP_612179.1.
UniGene	Mm.314923
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q80UP3. 1 interaction.
STRING	Q80UP3.
PTM databases
PhosphoSite	Q80UP3.
Proteomic databases
PRIDE	Q80UP3.
Genome annotation databases
Ensembl	ENSMUST00000028667; ENSMUSP00000028667; ENSMUSG00000040479; Mus musculus. [Genome view] ENSMUST00000111304; ENSMUSP00000106935; ENSMUSG00000040479; Mus musculus. [Genome view]
GeneID	104418.
KEGG	mmu:104418.
UCSC	uc008kxc.1. mouse.
Organism-specific databases
CTD	104418.
MGI	MGI:1278339. Dgkz.
Phylogenomic databases
HOGENOM	Q80UP3.
HOVERGEN	Q80UP3.
Enzyme and pathway databases
BRENDA	2.7.1.107. 244.
Gene expression databases
ArrayExpress	Q80UP3.
Bgee	Q80UP3.
CleanEx	MM_DGKZ.
Genevestigator	Q80UP3.
GermOnline	ENSMUSG00000040479. Mus musculus.
Family and domain databases
InterPro	IPR002110. ANK. IPR000756. Diacylglycerol_kinase_access. IPR001206. Diacylglycerol_kinase_cat. IPR002219. Prot_Kinase_C-like_PE/DAG_bd. [Graphical view]
Gene3D	G3DSA:1.25.40.20. ANK. 1 hit.
Pfam	PF00023. Ank. 2 hits. PF00130. C1_1. 1 hit. PF00609. DAGK_acc. 1 hit. PF00781. DAGK_cat. 1 hit. [Graphical view]
ProDom	PD002939. DAGKa. 1 hit. PD005043. DAGKc. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00248. ANK. 2 hits. SM00109. C1. 2 hits. SM00045. DAGKa. 1 hit. SM00046. DAGKc. 1 hit. [Graphical view]
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 2 hits. PS50146. DAGK. 1 hit. PS00479. ZF_DAG_PE_1. False negative. PS50081. ZF_DAG_PE_2. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	357101.
SOURCE	Search...

Entry information

Entry name

DGKZ_MOUSE

Accession

Primary (citable) accession number: Q80UP3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 22, 2003
Last sequence update:	June 1, 2003
Last modified:	October 13, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)