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Protein

Diacylglycerol kinase zeta

Gene

Dgkz

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Displays a strong preference for 1,2-diacylglycerols over 1,3-diacylglycerols, but lacks substrate specificity among molecular species of long chain diacylglycerols. Regulates RASGRP1 activity (By similarity).By similarity

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri98 – 15356Phorbol-ester/DAG-type 1Add
BLAST
Zinc fingeri173 – 23159Phorbol-ester/DAG-type 2Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. diacylglycerol kinase activity Source: MGI
  3. enzyme inhibitor activity Source: MGI
  4. lipid kinase activity Source: MGI
  5. metal ion binding Source: UniProtKB-KW
  6. NAD+ kinase activity Source: InterPro
  7. protein C-terminus binding Source: MGI

GO - Biological processi

  1. intracellular signal transduction Source: InterPro
  2. lipid phosphorylation Source: MGI
  3. mitotic G1 DNA damage checkpoint Source: MGI
  4. negative regulation of catalytic activity Source: GOC
  5. negative regulation of mitotic cell cycle Source: MGI
  6. negative regulation of Ras protein signal transduction Source: MGI
  7. phosphorylation Source: MGI
  8. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_219232. Effects of PIP2 hydrolysis.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase zeta (EC:2.7.1.107)
Short name:
DAG kinase zeta
Alternative name(s):
Diglyceride kinase zeta
Short name:
DGK-zeta
Gene namesi
Name:Dgkz
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:1278339. Dgkz.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication. Cell membrane By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. lamellipodium Source: MGI
  3. nucleus Source: MGI
  4. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 929929Diacylglycerol kinase zetaPRO_0000218469Add
BLAST

Proteomic databases

MaxQBiQ80UP3.
PaxDbiQ80UP3.
PRIDEiQ80UP3.

PTM databases

PhosphoSiteiQ80UP3.

Expressioni

Gene expression databases

BgeeiQ80UP3.
CleanExiMM_DGKZ.
ExpressionAtlasiQ80UP3. baseline and differential.
GenevestigatoriQ80UP3.

Interactioni

Subunit structurei

Interacts with the PDZ domain of the syntrophin SNTG1 and that of SNX27. Forms a signaling complex with RASGRP1 and HRAS (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Rb1P134052EBI-971774,EBI-971782

Protein-protein interaction databases

BioGridi222626. 8 interactions.
IntActiQ80UP3. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ80UP3.
SMRiQ80UP3. Positions 792-895.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini292 – 426135DAGKcPROSITE-ProRule annotationAdd
BLAST
Repeati823 – 85331ANK 1Add
BLAST
Repeati858 – 88730ANK 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni279 – 417139Mediates interaction with RASGRP1By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi362 – 3709Nuclear export signal

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi67 – 726Poly-Pro
Compositional biasi260 – 2634Poly-Lys
Compositional biasi372 – 3754Poly-Pro

Sequence similaritiesi

Contains 2 ANK repeats.PROSITE-ProRule annotation
Contains 1 DAGKc domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri98 – 15356Phorbol-ester/DAG-type 1Add
BLAST
Zinc fingeri173 – 23159Phorbol-ester/DAG-type 2Add
BLAST

Keywords - Domaini

ANK repeat, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG47311.
GeneTreeiENSGT00760000119050.
HOGENOMiHOG000231472.
HOVERGENiHBG067303.
InParanoidiQ80UP3.
KOiK00901.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR002219. PE/DAG-bd.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF00130. C1_1. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 2 hits.
SM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
SSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS50146. DAGK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q80UP3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEPRDPSPEG RSSDSESASA SSSGSERDAG PEPDKAPRRL TKRRFPGLRL
60 70 80 90 100
FGHRKAITKS GLQHLAPPPP TPGAPCGESE EQIQSTVDWS ESAVYGEHIW
110 120 130 140 150
FETNVSGDFC YVGEQHCVAK MLPKSAPRKK CAACKIVVHT QCIKQLEKIN
160 170 180 190 200
FRCKPSFRES GSRNVREPTF VRHHWVHRRR QDGKCRHCGK GFQQKFTFHS
210 220 230 240 250
KEIVAISCSW CKQAYHSKVS CFMMQQIEEP CSLGVHAAVV IPPTWILRAR
260 270 280 290 300
RPQNTLKASK KKKRASFKRR SSKKGPEEGR WRPFIIRPTP SPLMKPLLVF
310 320 330 340 350
VNPKSGGNQG AKIIQSFLWY LNPRQVFDLS QGGPREALEM YRKVHNLRIL
360 370 380 390 400
ACGGDGTVGW ILSTLDQLRL KPPPPVAILP LGTGNDLART LNWGGGYTDE
410 420 430 440 450
PVSKILSHVE EGNVVQLDRW DLRAEPNPEA GPEERDDGAT DRLPLDVFNN
460 470 480 490 500
YFSLGFDAHV TLEFHESREA NPEKFNSRFR NKMFYAGTAF SDFLMGSSKD
510 520 530 540 550
LAKHIRVVCD GMDLTPKIQD LKPQCIVFLN IPRYCAGTMP WGHPGEHHDF
560 570 580 590 600
EPQRHDDGYL EVIGFTMTSL AALQVGGHGE RLTQCREVLL TTAKAIPVQV
610 620 630 640 650
DGEPCKLSAS RIRIALRNQA TMVQKAKRRS TAPLHSDQQP VPEQLRIQVS
660 670 680 690 700
RVSMHDYEAL HYDKEQLKEA SVPLGTVVVP GDSDLELCRA HIERLQREPD
710 720 730 740 750
GAGAKSPMCH QLSSKWCFLD ATTASRFYRI DRAQEHLNYV TEIAQDEIYI
760 770 780 790 800
LDPELLGASA RPDLPTPTSP LPASPCSPTP GSMQGDTALP QGEELIEAAK
810 820 830 840 850
RNDCCKLQEL HRAGGDLMHR DQKSRTLLHH AVSTGSKEVV RYLLDHAPPE
860 870 880 890 900
ILDAVEENGE TCLHQAAALG QRTICHYIVE AGASLMKTDL QGDTPRQRAE
910 920
KAQDTELAAY LENRQHYQMI QREDQETAV
Length:929
Mass (Da):104,031
Last modified:July 27, 2011 - v2
Checksum:iAC3E48790E3C3463
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti908 – 9081A → S in AAH49228. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL731772, AL732484 Genomic DNA. Translation: CAM19275.1.
AL732484, AL731772 Genomic DNA. Translation: CAM22583.1.
BC049228 mRNA. Translation: AAH49228.1.
CCDSiCCDS16441.1.
RefSeqiNP_612179.2. NM_138306.2.
UniGeneiMm.314923.

Genome annotation databases

EnsembliENSMUST00000028667; ENSMUSP00000028667; ENSMUSG00000040479.
GeneIDi104418.
KEGGimmu:104418.
UCSCiuc008kxc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL731772, AL732484 Genomic DNA. Translation: CAM19275.1.
AL732484, AL731772 Genomic DNA. Translation: CAM22583.1.
BC049228 mRNA. Translation: AAH49228.1.
CCDSiCCDS16441.1.
RefSeqiNP_612179.2. NM_138306.2.
UniGeneiMm.314923.

3D structure databases

ProteinModelPortaliQ80UP3.
SMRiQ80UP3. Positions 792-895.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi222626. 8 interactions.
IntActiQ80UP3. 1 interaction.

PTM databases

PhosphoSiteiQ80UP3.

Proteomic databases

MaxQBiQ80UP3.
PaxDbiQ80UP3.
PRIDEiQ80UP3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028667; ENSMUSP00000028667; ENSMUSG00000040479.
GeneIDi104418.
KEGGimmu:104418.
UCSCiuc008kxc.2. mouse.

Organism-specific databases

CTDi8525.
MGIiMGI:1278339. Dgkz.

Phylogenomic databases

eggNOGiNOG47311.
GeneTreeiENSGT00760000119050.
HOGENOMiHOG000231472.
HOVERGENiHBG067303.
InParanoidiQ80UP3.
KOiK00901.

Enzyme and pathway databases

ReactomeiREACT_219232. Effects of PIP2 hydrolysis.

Miscellaneous databases

ChiTaRSiDgkz. mouse.
NextBioi357101.
PROiQ80UP3.
SOURCEiSearch...

Gene expression databases

BgeeiQ80UP3.
CleanExiMM_DGKZ.
ExpressionAtlasiQ80UP3. baseline and differential.
GenevestigatoriQ80UP3.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR002219. PE/DAG-bd.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF00130. C1_1. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 2 hits.
SM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
SSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS50146. DAGK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Mammary tumor.
  3. "Identification of a functional nuclear export sequence in diacylglycerol kinase-zeta."
    Evangelisti C., Gaboardi G.C., Billi A.M., Ognibene A., Goto K., Tazzari P.L., McCubrey J.A., Martelli A.M.
    Cell Cycle 9:384-388(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, NUCLEAR EXPORT SIGNAL.

Entry informationi

Entry nameiDGKZ_MOUSE
AccessioniPrimary (citable) accession number: Q80UP3
Secondary accession number(s): A2AHJ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 22, 2003
Last sequence update: July 27, 2011
Last modified: February 4, 2015
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.