Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA dimethylallyltransferase

Gene

Trit1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 of both cytosolic and mitochondrial tRNAs, leading to the formation of N6-(dimethylallyl)adenosine (i6A).By similarity

Catalytic activityi

Dimethylallyl diphosphate + adenine(37) in tRNA = diphosphate + N6-dimethylallyladenine(37) in tRNA.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri395 – 425Matrin-typeAdd BLAST31

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processtRNA processing
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA dimethylallyltransferase (EC:2.5.1.75)
Alternative name(s):
Isopentenyl-diphosphate:tRNA isopentenyltransferase
Short name:
IPP transferase
Short name:
IPPT
tRNA isopentenyltransferase
Short name:
IPTase
Gene namesi
Name:Trit1
Synonyms:Ipt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1914216 Trit1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 47MitochondrionSequence analysisAdd BLAST47
ChainiPRO_000001902448 – 467tRNA dimethylallyltransferaseAdd BLAST420

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei443PhosphoserineBy similarity1
Modified residuei455PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ80UN9
PaxDbiQ80UN9
PeptideAtlasiQ80UN9
PRIDEiQ80UN9

PTM databases

iPTMnetiQ80UN9
PhosphoSitePlusiQ80UN9

Expressioni

Gene expression databases

BgeeiENSMUSG00000028653
CleanExiMM_TRIT1
GenevisibleiQ80UN9 MM

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei122Interaction with substrate tRNABy similarity1
Sitei206Interaction with substrate tRNABy similarity1

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099709

Structurei

3D structure databases

ProteinModelPortaliQ80UN9
SMRiQ80UN9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni32 – 37Substrate bindingBy similarity6
Regioni55 – 58Interaction with substrate tRNABy similarity4
Regioni183 – 187Interaction with substrate tRNABy similarity5
Regioni233 – 255Interaction with isopentenylpyrophosphate transferaseBy similarityAdd BLAST23
Regioni281 – 283Interaction with substrate tRNABy similarity3
Regioni313 – 331Interaction with substrate tRNABy similarityAdd BLAST19
Regioni323 – 330Interaction with substrate tRNABy similarity8

Sequence similaritiesi

Belongs to the IPP transferase family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri395 – 425Matrin-typeAdd BLAST31

Keywords - Domaini

Transit peptide, Zinc-finger

Phylogenomic databases

eggNOGiKOG1384 Eukaryota
COG0324 LUCA
GeneTreeiENSGT00390000015214
HOGENOMiHOG000039995
HOVERGENiHBG052492
InParanoidiQ80UN9
KOiK00791
OMAiGTHYYTQ
OrthoDBiEOG091G0VY8
PhylomeDBiQ80UN9
TreeFamiTF315069

Family and domain databases

HAMAPiMF_00185 IPP_trans, 1 hit
InterProiView protein in InterPro
IPR030666 IPP_transferase_euk
IPR018022 IPT
IPR027417 P-loop_NTPase
IPR022755 Znf_C2H2_jaz
IPR036236 Znf_C2H2_sf
PfamiView protein in Pfam
PF01715 IPPT, 1 hit
PF12171 zf-C2H2_jaz, 1 hit
PIRSFiPIRSF039110 IPP_transferase, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
SSF57667 SSF57667, 1 hit
TIGRFAMsiTIGR00174 miaA, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80UN9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAAAARAV PVSSGFRGLR RTLPLVVILG ATGTGKSTLA LQLGQRLGGE
60 70 80 90 100
IVSADSMQVY EGLDIITNKV SAQEQKMCQH HMISFVDPLV TSYTVVDFRN
110 120 130 140 150
KATALIEDIF ARDKIPIVVG GTNYYIESLL WKVLITTKPQ EMGTGKVVDR
160 170 180 190 200
KVELEKEDGH ELHKRLSQVD PEMAAKLHPH DKRKVARSLQ VFEETGISHS
210 220 230 240 250
EFLHRQHAEE GGGPLGGPLR FPNPCILWLH ADQAVLDERL DKRVDDMLAA
260 270 280 290 300
GLLEELRGFH RRYNLKNISE NSQDYQHGIF QSIGFKEFHE YLTTEGKCTP
310 320 330 340 350
ETSNQLLKKG IEALKQVTKR YARKQNRWVK NRFLSRPGPS VPPVYGLEVS
360 370 380 390 400
DVSKWEESVL EPALNIVQSF IQGHKPTAMP VKMAYNESEN KRSYHMCDLC
410 420 430 440 450
DRIIIGDREW AAHLKSKSHL HQLKKRRRLD LDAVSATGSQ SNSPDCDPER
460
IEGESSGQHN QELKASV
Length:467
Mass (Da):52,437
Last modified:July 5, 2004 - v2
Checksum:iBC0EEF50AE05FD82
GO
Isoform 2 (identifier: Q80UN9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-141: Missing.

Show »
Length:326
Mass (Da):37,191
Checksum:i454367A8B70DD1F0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti379M → T in AAH51040 (PubMed:15489334).Curated1
Sequence conflicti384A → T in AAH51040 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0107221 – 141Missing in isoform 2. 2 PublicationsAdd BLAST141

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003556 mRNA Translation: BAB22853.2
AL606906 Genomic DNA No translation available.
BC019812 mRNA Translation: AAH19812.1
BC051040 mRNA Translation: AAH51040.1
CCDSiCCDS18607.1 [Q80UN9-1]
RefSeqiNP_080149.2, NM_025873.2 [Q80UN9-1]
UniGeneiMm.235030

Genome annotation databases

EnsembliENSMUST00000102649; ENSMUSP00000099709; ENSMUSG00000028653 [Q80UN9-1]
GeneIDi66966
KEGGimmu:66966
UCSCiuc008uor.1 mouse [Q80UN9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMOD5_MOUSE
AccessioniPrimary (citable) accession number: Q80UN9
Secondary accession number(s): B1ARS6, Q9D1H5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health