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Protein

tRNA dimethylallyltransferase, mitochondrial

Gene

Trit1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 of both cytosolic and mitochondrial tRNAs, leading to the formation of N6-(dimethylallyl)adenosine (i6A).By similarity

Catalytic activityi

Dimethylallyl diphosphate + adenine(37) in tRNA = diphosphate + N(6)-dimethylallyladenine(37) in tRNA.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri395 – 425Matrin-typeAdd BLAST31

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA dimethylallyltransferase, mitochondrial (EC:2.5.1.75)
Alternative name(s):
Isopentenyl-diphosphate:tRNA isopentenyltransferase
Short name:
IPP transferase
Short name:
IPPT
tRNA isopentenyltransferase
Short name:
IPTase
Gene namesi
Name:Trit1
Synonyms:Ipt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1914216. Trit1.

Subcellular locationi

Isoform 2 :

GO - Cellular componenti

  • mitochondrion Source: MGI
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 47MitochondrionSequence analysisAdd BLAST47
ChainiPRO_000001902448 – 467tRNA dimethylallyltransferase, mitochondrialAdd BLAST420

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei443PhosphoserineBy similarity1
Modified residuei455PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ80UN9.
PaxDbiQ80UN9.
PeptideAtlasiQ80UN9.
PRIDEiQ80UN9.

PTM databases

PhosphoSitePlusiQ80UN9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028653.
CleanExiMM_TRIT1.
GenevisibleiQ80UN9. MM.

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei122Interaction with substrate tRNABy similarity1
Sitei206Interaction with substrate tRNABy similarity1

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099709.

Structurei

3D structure databases

ProteinModelPortaliQ80UN9.
SMRiQ80UN9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni32 – 37Substrate bindingBy similarity6
Regioni55 – 58Interaction with substrate tRNABy similarity4
Regioni183 – 187Interaction with substrate tRNABy similarity5
Regioni233 – 255Interaction with isopentenylpyrophosphate transferaseBy similarityAdd BLAST23
Regioni281 – 283Interaction with substrate tRNABy similarity3
Regioni313 – 331Interaction with substrate tRNABy similarityAdd BLAST19
Regioni323 – 330Interaction with substrate tRNABy similarity8

Sequence similaritiesi

Belongs to the IPP transferase family.Curated
Contains 1 matrin-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri395 – 425Matrin-typeAdd BLAST31

Keywords - Domaini

Transit peptide, Zinc-finger

Phylogenomic databases

eggNOGiKOG1384. Eukaryota.
COG0324. LUCA.
GeneTreeiENSGT00390000015214.
HOGENOMiHOG000039995.
HOVERGENiHBG052492.
InParanoidiQ80UN9.
KOiK00791.
OMAiPHHMISF.
OrthoDBiEOG091G0VY8.
PhylomeDBiQ80UN9.
TreeFamiTF315069.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans. 1 hit.
InterProiIPR018022. IPP_trans.
IPR030666. IPP_transferase_euk.
IPR027417. P-loop_NTPase.
IPR022755. Znf_C2H2_jaz.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
PF12171. zf-C2H2_jaz. 1 hit.
[Graphical view]
PIRSFiPIRSF039110. IPP_transferase. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00174. miaA. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80UN9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAAAARAV PVSSGFRGLR RTLPLVVILG ATGTGKSTLA LQLGQRLGGE
60 70 80 90 100
IVSADSMQVY EGLDIITNKV SAQEQKMCQH HMISFVDPLV TSYTVVDFRN
110 120 130 140 150
KATALIEDIF ARDKIPIVVG GTNYYIESLL WKVLITTKPQ EMGTGKVVDR
160 170 180 190 200
KVELEKEDGH ELHKRLSQVD PEMAAKLHPH DKRKVARSLQ VFEETGISHS
210 220 230 240 250
EFLHRQHAEE GGGPLGGPLR FPNPCILWLH ADQAVLDERL DKRVDDMLAA
260 270 280 290 300
GLLEELRGFH RRYNLKNISE NSQDYQHGIF QSIGFKEFHE YLTTEGKCTP
310 320 330 340 350
ETSNQLLKKG IEALKQVTKR YARKQNRWVK NRFLSRPGPS VPPVYGLEVS
360 370 380 390 400
DVSKWEESVL EPALNIVQSF IQGHKPTAMP VKMAYNESEN KRSYHMCDLC
410 420 430 440 450
DRIIIGDREW AAHLKSKSHL HQLKKRRRLD LDAVSATGSQ SNSPDCDPER
460
IEGESSGQHN QELKASV
Length:467
Mass (Da):52,437
Last modified:July 5, 2004 - v2
Checksum:iBC0EEF50AE05FD82
GO
Isoform 2 (identifier: Q80UN9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-141: Missing.

Show »
Length:326
Mass (Da):37,191
Checksum:i454367A8B70DD1F0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti379M → T in AAH51040 (PubMed:15489334).Curated1
Sequence conflicti384A → T in AAH51040 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0107221 – 141Missing in isoform 2. 2 PublicationsAdd BLAST141

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003556 mRNA. Translation: BAB22853.2.
AL606906 Genomic DNA. Translation: CAM14000.1.
BC019812 mRNA. Translation: AAH19812.1.
BC051040 mRNA. Translation: AAH51040.1.
CCDSiCCDS18607.1. [Q80UN9-1]
RefSeqiNP_080149.2. NM_025873.2. [Q80UN9-1]
UniGeneiMm.235030.

Genome annotation databases

EnsembliENSMUST00000102649; ENSMUSP00000099709; ENSMUSG00000028653. [Q80UN9-1]
GeneIDi66966.
KEGGimmu:66966.
UCSCiuc008uor.1. mouse. [Q80UN9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003556 mRNA. Translation: BAB22853.2.
AL606906 Genomic DNA. Translation: CAM14000.1.
BC019812 mRNA. Translation: AAH19812.1.
BC051040 mRNA. Translation: AAH51040.1.
CCDSiCCDS18607.1. [Q80UN9-1]
RefSeqiNP_080149.2. NM_025873.2. [Q80UN9-1]
UniGeneiMm.235030.

3D structure databases

ProteinModelPortaliQ80UN9.
SMRiQ80UN9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099709.

PTM databases

PhosphoSitePlusiQ80UN9.

Proteomic databases

EPDiQ80UN9.
PaxDbiQ80UN9.
PeptideAtlasiQ80UN9.
PRIDEiQ80UN9.

Protocols and materials databases

DNASUi66966.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102649; ENSMUSP00000099709; ENSMUSG00000028653. [Q80UN9-1]
GeneIDi66966.
KEGGimmu:66966.
UCSCiuc008uor.1. mouse. [Q80UN9-1]

Organism-specific databases

CTDi54802.
MGIiMGI:1914216. Trit1.

Phylogenomic databases

eggNOGiKOG1384. Eukaryota.
COG0324. LUCA.
GeneTreeiENSGT00390000015214.
HOGENOMiHOG000039995.
HOVERGENiHBG052492.
InParanoidiQ80UN9.
KOiK00791.
OMAiPHHMISF.
OrthoDBiEOG091G0VY8.
PhylomeDBiQ80UN9.
TreeFamiTF315069.

Miscellaneous databases

PROiQ80UN9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028653.
CleanExiMM_TRIT1.
GenevisibleiQ80UN9. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans. 1 hit.
InterProiIPR018022. IPP_trans.
IPR030666. IPP_transferase_euk.
IPR027417. P-loop_NTPase.
IPR022755. Znf_C2H2_jaz.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
PF12171. zf-C2H2_jaz. 1 hit.
[Graphical view]
PIRSFiPIRSF039110. IPP_transferase. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00174. miaA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMOD5_MOUSE
AccessioniPrimary (citable) accession number: Q80UN9
Secondary accession number(s): B1ARS6, Q9D1H5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.