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Q80UM3

- NAA15_MOUSE

UniProt

Q80UM3 - NAA15_MOUSE

Protein

N-alpha-acetyltransferase 15, NatA auxiliary subunit

Gene

Naa15

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Auxillary subunit of the N-terminal acetyltransferase A (NatA) complex which displays alpha (N-terminal) acetyltransferase activity. The NAT activity may be important for vascular, hematopoietic and neuronal growth and development. Required to control retinal neovascularization in adult ocular endothelial cells. In complex with XRCC6 and XRCC5 (Ku80), up-regulates transcription from the osteocalcin promoter.4 Publications

    GO - Molecular functioni

    1. N-acetyltransferase activity Source: MGI
    2. protein binding Source: MGI

    GO - Biological processi

    1. angiogenesis Source: UniProtKB-KW
    2. cell differentiation Source: UniProtKB-KW
    3. regulation of transcription, DNA-templated Source: UniProtKB-KW
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Angiogenesis, Differentiation, Transcription, Transcription regulation

    Enzyme and pathway databases

    BRENDAi2.3.1.88. 3474.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-alpha-acetyltransferase 15, NatA auxiliary subunit
    Alternative name(s):
    N-terminal acetyltransferase 1
    NMDA receptor-regulated protein 1
    Protein tubedown-1
    Gene namesi
    Name:Naa15
    Synonyms:Narg1, Nat1, Tbdn-1, Tubedown
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:1922088. Naa15.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity. Cytoplasmperinuclear region By similarity
    Note: Mainly cytoplasmic, nuclear in some cases. Present in the free cytosolic and cytoskeleton-bound polysomes, but not in the membrane-bound polysomes.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. nucleus Source: UniProtKB-SubCell
    3. perinuclear region of cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 865865N-alpha-acetyltransferase 15, NatA auxiliary subunitPRO_0000106295Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei262 – 2621N6-acetyllysineBy similarity
    Modified residuei399 – 3991PhosphothreonineBy similarity
    Modified residuei403 – 4031PhosphoserineBy similarity
    Modified residuei588 – 5881PhosphoserineBy similarity
    Modified residuei734 – 7341N6-acetyllysineBy similarity
    Modified residuei755 – 7551N6-acetyllysineBy similarity
    Modified residuei854 – 8541Phosphoserine1 Publication
    Modified residuei855 – 8551Phosphoserine1 Publication

    Post-translational modificationi

    Acetylated.1 Publication
    Cleaved by caspases during apoptosis.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ80UM3.
    PaxDbiQ80UM3.
    PRIDEiQ80UM3.

    PTM databases

    PhosphoSiteiQ80UM3.

    Expressioni

    Tissue specificityi

    Endothelial cells, osteoblasts and myeloid cells of the hematopoietic tissue. Present in adult ovary, bone marrow, brain, heart, kidney, testis and osteoblasts.3 Publications

    Developmental stagei

    Highly expressed in endothelial cells during embryonic vasculogenesis, and then down-regulated and restricted to specific endothelial cells. In the brain, expression is highest in regions that contain dividing and proliferating cells. As brain development progresses, expression restricts to the hippocampus and cerebellar cortex.1 Publication

    Inductioni

    Regulated by NMDA receptor.1 Publication

    Gene expression databases

    CleanExiMM_NARG1.
    GenevestigatoriQ80UM3.

    Interactioni

    Subunit structurei

    Component of the N-terminal acetyltransferase A (NatA) complex composed of NAA10 or probably NAA11 and NAA15. Interacts with XRCC6, NAA50 and XRCC5. Associates with HYPK when in a complex with NAA10.2 Publications

    Protein-protein interaction databases

    IntActiQ80UM3. 4 interactions.
    MINTiMINT-1865089.

    Structurei

    3D structure databases

    ProteinModelPortaliQ80UM3.
    SMRiQ80UM3. Positions 1-778.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati46 – 7934TPR 1Add
    BLAST
    Repeati80 – 11334TPR 2Add
    BLAST
    Repeati148 – 18437TPR 3Add
    BLAST
    Repeati224 – 25734TPR 4Add
    BLAST
    Repeati374 – 40734TPR 5Add
    BLAST
    Repeati409 – 44133TPR 6Add
    BLAST
    Repeati485 – 51834TPR 7Add
    BLAST
    Repeati672 – 70534TPR 8Add
    BLAST
    Repeati799 – 83436TPR 9Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni500 – 865366Interaction with HYPKBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi612 – 62918Bipartite nuclear localization signalSequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi629 – 6324Poly-Asp

    Sequence similaritiesi

    Contains 9 TPR repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, TPR repeat

    Phylogenomic databases

    eggNOGiCOG0457.
    HOGENOMiHOG000191711.
    HOVERGENiHBG052576.
    InParanoidiQ80UM3.
    PhylomeDBiQ80UM3.

    Family and domain databases

    Gene3Di1.25.40.10. 3 hits.
    InterProiIPR021183. NatA_aux_su.
    IPR013026. TPR-contain_dom.
    IPR011990. TPR-like_helical.
    IPR013105. TPR_2.
    IPR019734. TPR_repeat.
    [Graphical view]
    PfamiPF12569. NARP1. 1 hit.
    PF07719. TPR_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000422. N-terminal-AcTrfase-A_aux_su. 1 hit.
    SMARTiSM00028. TPR. 4 hits.
    [Graphical view]
    PROSITEiPS50005. TPR. 5 hits.
    PS50293. TPR_REGION. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q80UM3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPAVSLPPKE NALFKRILRC YEHKQYRNGL KFCKQILSNP KFAEHGETLA    50
    MKGLTLNCLG KKEEAYELVR RGLRNDLKSH VCWHVYGLLQ RSDKKYDEAI 100
    KCYRNALKWD KDNLQILRDL SLLQIQMRDL EGYRETRYQL LQLRPAQRAS 150
    WIGYAIAYHL LEDYEMAAKI LEEFRKTQQT SPDKVDYEYS ELLLYQNQVL 200
    REAGLYREAL EHLCTYEKQI CDKLAVEETK GELLLQLCRL EDAADVYRGL 250
    QERNPGNWAY YKGLEKALKP ANMLERLKIY EEAWTKYPRG LVPRRLPLNF 300
    LSGEKFKECL DRFLRMNFSK GCPPVFNTLR SLYRDKEKVA IVEELVVGYE 350
    TSLKSCRLFN PNDDGKEEPP TTLLWVQYYL AQHYDKIGQP SIALEYINTA 400
    IESTPTLIEL FLVKAKIYKH AGNIKEAARW MDEAQALDTA DRFINSKCAK 450
    YVLKANLIKE AEEMCSKFTR EGTSAVENLN EMQCMWFQTE CAQAYKAMNK 500
    FGEALKKCHE IERHFIEITD DQFDFHTYCM RKITLRSYVD LLKLEDVLRQ 550
    HPFYFKAARI AIEIYLKLHD NPLTDENKEH EADTANMSDK ELKKLRNKQR 600
    RAQKKAQIEE EKKNAEKEKQ QRNQKKKKDD DDEEIGGPKE ELIPEKLAKV 650
    ETPLEEAIKF LTPLKNLVKN KIETHLFAFE IYFRKEKFLL MLQSVKRAFA 700
    IDSGHPWLHE CMIRLFHSVC ESKDLPETVR TVLKQEMNRL FGATNPKNFN 750
    ETFLKRNSDS LPHRLSAAKM VYYLDSSSQK RAIELATTLD GSLTNRNLQT 800
    CMEVLEALCD GSLGDCKEAA EAYRASCHKL FPYALAFMPP GYEEDMKITV 850
    NGDSSAETEE LANEI 865
    Length:865
    Mass (Da):100,961
    Last modified:June 1, 2003 - v1
    Checksum:i230C5B6EE8697440
    GO

    Sequence cautioni

    The sequence AAF73953.2 differs from that shown. Reason: Contaminating sequence.
    The sequence AAF73953.2 differs from that shown. Reason: Frameshift at several positions.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti256 – 2561G → E(PubMed:12888564)Curated
    Sequence conflicti256 – 2561G → E(PubMed:10842358)Curated
    Sequence conflicti295 – 2951R → K(PubMed:12888564)Curated
    Sequence conflicti295 – 2951R → K(PubMed:10842358)Curated
    Sequence conflicti452 – 4521V → M(PubMed:12888564)Curated
    Sequence conflicti452 – 4521V → M(PubMed:10842358)Curated
    Sequence conflicti620 – 6201Q → P in AAF73953. (PubMed:10842358)Curated
    Sequence conflicti624 – 6241Q → P in AAF73953. (PubMed:10842358)Curated
    Sequence conflicti704 – 7041G → S(PubMed:12888564)Curated
    Sequence conflicti704 – 7041G → S(PubMed:10842358)Curated
    Sequence conflicti814 – 8141G → R in AAF73953. (PubMed:10842358)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF510858 mRNA. Translation: AAO33713.1.
    BC050017 mRNA. Translation: AAH50017.1.
    AF237622 mRNA. Translation: AAF73953.2. Sequence problems.
    CCDSiCCDS17340.1.
    UniGeneiMm.275281.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF510858 mRNA. Translation: AAO33713.1 .
    BC050017 mRNA. Translation: AAH50017.1 .
    AF237622 mRNA. Translation: AAF73953.2 . Sequence problems.
    CCDSi CCDS17340.1.
    UniGenei Mm.275281.

    3D structure databases

    ProteinModelPortali Q80UM3.
    SMRi Q80UM3. Positions 1-778.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q80UM3. 4 interactions.
    MINTi MINT-1865089.

    PTM databases

    PhosphoSitei Q80UM3.

    Proteomic databases

    MaxQBi Q80UM3.
    PaxDbi Q80UM3.
    PRIDEi Q80UM3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    MGIi MGI:1922088. Naa15.

    Phylogenomic databases

    eggNOGi COG0457.
    HOGENOMi HOG000191711.
    HOVERGENi HBG052576.
    InParanoidi Q80UM3.
    PhylomeDBi Q80UM3.

    Enzyme and pathway databases

    BRENDAi 2.3.1.88. 3474.

    Miscellaneous databases

    PROi Q80UM3.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_NARG1.
    Genevestigatori Q80UM3.

    Family and domain databases

    Gene3Di 1.25.40.10. 3 hits.
    InterProi IPR021183. NatA_aux_su.
    IPR013026. TPR-contain_dom.
    IPR011990. TPR-like_helical.
    IPR013105. TPR_2.
    IPR019734. TPR_repeat.
    [Graphical view ]
    Pfami PF12569. NARP1. 1 hit.
    PF07719. TPR_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000422. N-terminal-AcTrfase-A_aux_su. 1 hit.
    SMARTi SM00028. TPR. 4 hits.
    [Graphical view ]
    PROSITEi PS50005. TPR. 5 hits.
    PS50293. TPR_REGION. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Regulation of osteocalcin gene expression by a novel Ku antigen transcription factor complex."
      Willis D.M., Loewy A.P., Charlton-Kachigian N., Shao J.-S., Ornitz D.M., Towler D.A.
      J. Biol. Chem. 277:37280-37291(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH XRCC6 AND XRCC5, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, FUNCTION.
      Tissue: Heart.
    2. "An evolutionarily conserved N-terminal acetyltransferase complex associated with neuronal development."
      Sugiura N., Adams S.M., Corriveau R.A.
      J. Biol. Chem. 278:40113-40120(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH NAA10.
      Tissue: Testis.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: ICR.
      Tissue: Trophoblast stem cell.
    4. "Tubedown-1, a novel acetyltransferase associated with blood vessel development."
      Gendron R.L., Adams L.C., Paradis H.
      Dev. Dyn. 218:300-315(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 158-865, CHARACTERIZATION, ACETYLATION, FUNCTION.
      Strain: ICR.
      Tissue: Embryo.
    5. "N-methyl-D-aspartate receptors regulate a group of transiently expressed genes in the developing brain."
      Sugiura N., Patel R.G., Corriveau R.A.
      J. Biol. Chem. 276:14257-14263(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION, INDUCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    6. "Conditional knockdown of tubedown-1 in endothelial cells leads to neovascular retinopathy."
      Wall D.S., Gendron R.L., Good W.V., Miskiewicz E., Woodland M., Leblanc K., Paradis H.
      Invest. Ophthalmol. Vis. Sci. 45:3704-3712(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-854 AND SER-855, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiNAA15_MOUSE
    AccessioniPrimary (citable) accession number: Q80UM3
    Secondary accession number(s): Q811Z9, Q9JID5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 4, 2005
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3