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Q80UK7

- SAS6_MOUSE

UniProt

Q80UK7 - SAS6_MOUSE

Protein

Spindle assembly abnormal protein 6 homolog

Gene

Sass6

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Central scaffolding component of the centrioles ensuring their 9-fold symmetry. Required for centrosome biogenesis and duplication: required both for mother-centriole-dependent centriole duplication and deuterosome-dependent centriole amplification in multiciliated cells.1 Publication

    GO - Biological processi

    1. centriole replication Source: Ensembl

    Keywords - Biological processi

    Cell cycle

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Spindle assembly abnormal protein 6 homolog
    Gene namesi
    Name:Sass6
    Synonyms:Sas6
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:1920026. Sass6.

    Subcellular locationi

    Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosomecentriole By similarity
    Note: Component of the centrosome. Associated only transiently with nascent procentrioles during centriole biogenesis By similarity. Component of the deuterosome, a structure that promotes de novo centriole amplification in multiciliated cells that can generate more than 100 centrioles.By similarity1 Publication

    GO - Cellular componenti

    1. centriole Source: UniProtKB
    2. deuterosome Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 654654Spindle assembly abnormal protein 6 homologPRO_0000189973Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei509 – 5091PhosphoserineBy similarity
    Modified residuei654 – 6541PhosphoserineBy similarity

    Post-translational modificationi

    Ubiquitinated by the SCF(FBXW5) E3 ubiquitin-protein ligase complex during S phase, leading to its degradation and preventing centriole reduplication.By similarity

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    PRIDEiQ80UK7.

    PTM databases

    PhosphoSiteiQ80UK7.

    Expressioni

    Gene expression databases

    BgeeiQ80UK7.
    CleanExiMM_SASS6.
    GenevestigatoriQ80UK7.

    Interactioni

    Subunit structurei

    Nine homodimers form a cartwheel structure with an internal diameter of 23 nM and radial spokes connecting to the microtubule triplets. Part of a ternary complex composed of SASS6, CENPJ and CEP350 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi215561. 13 interactions.
    IntActiQ80UK7. 14 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ80UK7.
    SMRiQ80UK7. Positions 1-171.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini39 – 9153PISAAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili175 – 471297Sequence AnalysisAdd
    BLAST

    Domaini

    The 35 nM long coiled-coil domain mediates homodimerization while the globular N-terminus links the dimers at an angle of 40 degrees to form the inner ring.By similarity

    Sequence similaritiesi

    Contains 1 PISA domain.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG117813.
    GeneTreeiENSGT00390000006932.
    HOGENOMiHOG000124668.
    HOVERGENiHBG079167.
    InParanoidiQ80UK7.
    KOiK16487.
    OMAiFLASCLK.
    OrthoDBiEOG764727.
    TreeFamiTF326199.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q80UK7-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSQVLFQQLV PLLVKCKDCE ERRGSVRVSI ELQSLSNPVH RKDLVIRLTD    50
    DTDPFFLYNL VISEEDFQSL KLQQGLLVDF LAFPQKFIDL LQQCMQEHAK 100
    ETPRFLLQLL SSATLLENSP VLLNVVETNP FKHLIHLSLK LLPGNDVEIK 150
    KFLAGCLKCS KEEKLSLTRS LDDVTRQLHI TQETLSEKMQ ELDKLRSEWA 200
    SHTASLTNKH SQELTAEKEK ALQTQVQCQQ QHEQQKKELE TLHQRNIHQL 250
    QSRLSELEAA NKELTERKYK GDSTVRELKA KLAGVEEELQ RAKQEVLSLR 300
    RENCTLDTEC HEKEKHINQL QTKVAVLEQE IKDKDQLVLR TKEAFDTIQE 350
    QKVALEENGE KNQIQLGKLE ATIKSLSAEL LKANEIIKKL QGDLKTLMGK 400
    LKLKNTVTIQ QEKLLAEKEE MLQKERKESQ DAGQFLRAKE QEVCRLQEQL 450
    ETTVQKLEES KQLLKNNEKL ITWLNKELNE NQLVRKQDTL GTSATPHSTS 500
    NSTIRSGLSP NLNVVDRLNY PSCGIGYPVS SALTFQNAFP HVVAAKNTSH 550
    PISGPKVHFN LQLTKPSASI DGQPGAAVNR PCSNDKENGE TLGLESKYLK 600
    RREASIPLRG LSQNLLSDSD HQKDGMLGAF QLSSKPTVLP SSSSAYFPGQ 650
    LPSS 654
    Length:654
    Mass (Da):74,054
    Last modified:July 27, 2011 - v2
    Checksum:iBB83293FBC00DD42
    GO
    Isoform 2 (identifier: Q80UK7-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         558-560: HFN → AKC
         561-654: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:560
    Mass (Da):64,002
    Checksum:iF0B854FE0FC5B79C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti19 – 191C → R in BAB28799. (PubMed:16141072)Curated
    Sequence conflicti395 – 3951K → N in BAB28799. (PubMed:16141072)Curated
    Sequence conflicti579 – 5791N → D in AAH50110. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei558 – 5603HFN → AKC in isoform 2. 1 PublicationVSP_013318
    Alternative sequencei561 – 65494Missing in isoform 2. 1 PublicationVSP_013319Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK013338 mRNA. Translation: BAB28799.1.
    AC131038 Genomic DNA. No translation available.
    BC050110 mRNA. Translation: AAH50110.1.
    CCDSiCCDS17789.1. [Q80UK7-1]
    RefSeqiNP_001276497.1. NM_001289568.1.
    NP_001276500.1. NM_001289571.1.
    NP_082625.2. NM_028349.3. [Q80UK7-1]
    UniGeneiMm.440836.

    Genome annotation databases

    EnsembliENSMUST00000029571; ENSMUSP00000029571; ENSMUSG00000027959. [Q80UK7-1]
    GeneIDi72776.
    KEGGimmu:72776.
    UCSCiuc008rcm.1. mouse. [Q80UK7-2]
    uc008rcn.2. mouse. [Q80UK7-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK013338 mRNA. Translation: BAB28799.1 .
    AC131038 Genomic DNA. No translation available.
    BC050110 mRNA. Translation: AAH50110.1 .
    CCDSi CCDS17789.1. [Q80UK7-1 ]
    RefSeqi NP_001276497.1. NM_001289568.1.
    NP_001276500.1. NM_001289571.1.
    NP_082625.2. NM_028349.3. [Q80UK7-1 ]
    UniGenei Mm.440836.

    3D structure databases

    ProteinModelPortali Q80UK7.
    SMRi Q80UK7. Positions 1-171.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 215561. 13 interactions.
    IntActi Q80UK7. 14 interactions.

    PTM databases

    PhosphoSitei Q80UK7.

    Proteomic databases

    PRIDEi Q80UK7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000029571 ; ENSMUSP00000029571 ; ENSMUSG00000027959 . [Q80UK7-1 ]
    GeneIDi 72776.
    KEGGi mmu:72776.
    UCSCi uc008rcm.1. mouse. [Q80UK7-2 ]
    uc008rcn.2. mouse. [Q80UK7-1 ]

    Organism-specific databases

    CTDi 163786.
    MGIi MGI:1920026. Sass6.

    Phylogenomic databases

    eggNOGi NOG117813.
    GeneTreei ENSGT00390000006932.
    HOGENOMi HOG000124668.
    HOVERGENi HBG079167.
    InParanoidi Q80UK7.
    KOi K16487.
    OMAi FLASCLK.
    OrthoDBi EOG764727.
    TreeFami TF326199.

    Miscellaneous databases

    NextBioi 336909.
    PROi Q80UK7.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q80UK7.
    CleanExi MM_SASS6.
    Genevestigatori Q80UK7.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6J.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: ICR.
      Tissue: Trophoblast stem cell.
    4. "The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis."
      Zhao H., Zhu L., Zhu Y., Cao J., Li S., Huang Q., Xu T., Huang X., Yan X., Zhu X.
      Nat. Cell Biol. 15:1434-1444(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiSAS6_MOUSE
    AccessioniPrimary (citable) accession number: Q80UK7
    Secondary accession number(s): Q9CYT4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 12, 2005
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 83 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3