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Protein

Spindle assembly abnormal protein 6 homolog

Gene

Sass6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Central scaffolding component of the centrioles ensuring their 9-fold symmetry. Required for centrosome biogenesis and duplication: required both for mother-centriole-dependent centriole duplication and deuterosome-dependent centriole amplification in multiciliated cells (PubMed:24240477). Required for the recruitment of STIL to the procentriole and for STIL-mediated centriole amplification (By similarity).By similarity1 Publication

GO - Biological processi

Keywordsi

Biological processCell cycle

Names & Taxonomyi

Protein namesi
Recommended name:
Spindle assembly abnormal protein 6 homolog
Gene namesi
Name:Sass6
Synonyms:Sas6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1920026 Sass6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001899731 – 654Spindle assembly abnormal protein 6 homologAdd BLAST654

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei509PhosphoserineBy similarity1
Modified residuei612PhosphoserineBy similarity1
Modified residuei654PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated by the SCF(FBXW5) E3 ubiquitin-protein ligase complex during S phase, leading to its degradation and preventing centriole reduplication.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ80UK7
PeptideAtlasiQ80UK7
PRIDEiQ80UK7

PTM databases

iPTMnetiQ80UK7
PhosphoSitePlusiQ80UK7

Expressioni

Gene expression databases

BgeeiENSMUSG00000027959
CleanExiMM_SASS6
ExpressionAtlasiQ80UK7 baseline and differential
GenevisibleiQ80UK7 MM

Interactioni

Subunit structurei

Nine homodimers form a cartwheel structure with an internal diameter of 23 nM and radial spokes connecting to the microtubule triplets. Interacts with FBXW5. Forms a complex with CENPJ and STIL. Interacts with NUP62 and TUBG1 at the centrosome.By similarity

Protein-protein interaction databases

BioGridi215561, 20 interactors
IntActiQ80UK7, 21 interactors
STRINGi10090.ENSMUSP00000029571

Structurei

3D structure databases

ProteinModelPortaliQ80UK7
SMRiQ80UK7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 91PISAAdd BLAST53

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili175 – 471Sequence analysisAdd BLAST297

Domaini

The 35 nM long coiled-coil domain mediates homodimerization while the globular N-terminus links the dimers at an angle of 40 degrees to form the inner ring.By similarity

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IF03 Eukaryota
ENOG410XQWJ LUCA
GeneTreeiENSGT00390000006932
HOGENOMiHOG000124668
HOVERGENiHBG079167
InParanoidiQ80UK7
KOiK16487
OMAiKQDVLGP
OrthoDBiEOG091G09W4
TreeFamiTF326199

Family and domain databases

Gene3Di2.170.210.20, 1 hit
InterProiView protein in InterPro
IPR032396 SAS-6_N
IPR038558 SAS-6_N_sf
PfamiView protein in Pfam
PF16531 SAS-6_N, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80UK7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQVLFQQLV PLLVKCKDCE ERRGSVRVSI ELQSLSNPVH RKDLVIRLTD
60 70 80 90 100
DTDPFFLYNL VISEEDFQSL KLQQGLLVDF LAFPQKFIDL LQQCMQEHAK
110 120 130 140 150
ETPRFLLQLL SSATLLENSP VLLNVVETNP FKHLIHLSLK LLPGNDVEIK
160 170 180 190 200
KFLAGCLKCS KEEKLSLTRS LDDVTRQLHI TQETLSEKMQ ELDKLRSEWA
210 220 230 240 250
SHTASLTNKH SQELTAEKEK ALQTQVQCQQ QHEQQKKELE TLHQRNIHQL
260 270 280 290 300
QSRLSELEAA NKELTERKYK GDSTVRELKA KLAGVEEELQ RAKQEVLSLR
310 320 330 340 350
RENCTLDTEC HEKEKHINQL QTKVAVLEQE IKDKDQLVLR TKEAFDTIQE
360 370 380 390 400
QKVALEENGE KNQIQLGKLE ATIKSLSAEL LKANEIIKKL QGDLKTLMGK
410 420 430 440 450
LKLKNTVTIQ QEKLLAEKEE MLQKERKESQ DAGQFLRAKE QEVCRLQEQL
460 470 480 490 500
ETTVQKLEES KQLLKNNEKL ITWLNKELNE NQLVRKQDTL GTSATPHSTS
510 520 530 540 550
NSTIRSGLSP NLNVVDRLNY PSCGIGYPVS SALTFQNAFP HVVAAKNTSH
560 570 580 590 600
PISGPKVHFN LQLTKPSASI DGQPGAAVNR PCSNDKENGE TLGLESKYLK
610 620 630 640 650
RREASIPLRG LSQNLLSDSD HQKDGMLGAF QLSSKPTVLP SSSSAYFPGQ

LPSS
Length:654
Mass (Da):74,054
Last modified:July 27, 2011 - v2
Checksum:iBB83293FBC00DD42
GO
Isoform 2 (identifier: Q80UK7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     558-560: HFN → AKC
     561-654: Missing.

Note: No experimental confirmation available.
Show »
Length:560
Mass (Da):64,002
Checksum:iF0B854FE0FC5B79C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19C → R in BAB28799 (PubMed:16141072).Curated1
Sequence conflicti395K → N in BAB28799 (PubMed:16141072).Curated1
Sequence conflicti579N → D in AAH50110 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013318558 – 560HFN → AKC in isoform 2. 1 Publication3
Alternative sequenceiVSP_013319561 – 654Missing in isoform 2. 1 PublicationAdd BLAST94

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013338 mRNA Translation: BAB28799.1
AC131038 Genomic DNA No translation available.
BC050110 mRNA Translation: AAH50110.1
CCDSiCCDS17789.1 [Q80UK7-1]
RefSeqiNP_001276497.1, NM_001289568.1
NP_001276500.1, NM_001289571.1
NP_082625.2, NM_028349.3 [Q80UK7-1]
UniGeneiMm.440836

Genome annotation databases

EnsembliENSMUST00000198311; ENSMUSP00000143233; ENSMUSG00000027959 [Q80UK7-1]
ENSMUST00000198386; ENSMUSP00000143175; ENSMUSG00000027959 [Q80UK7-2]
GeneIDi72776
KEGGimmu:72776
UCSCiuc008rcm.2 mouse [Q80UK7-2]
uc008rcn.3 mouse [Q80UK7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSAS6_MOUSE
AccessioniPrimary (citable) accession number: Q80UK7
Secondary accession number(s): Q9CYT4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: July 27, 2011
Last modified: March 28, 2018
This is version 108 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health