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Protein

Solute carrier family 22 member 20

Gene

Slc22a20

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Organic anion transporter that mediates the uptake of estrone sulfate. Inhibited by probenecid, propionate, 2-methylbutyrate, 3-methylbutyrate, benzoate, heptanoate and 2-ethylhaxanoate. May act as an odorant transporter.2 Publications

Kineticsi

  1. KM=109.8 µM for estrone sulfate3 Publications
  1. Vmax=40 pmol/h/mg enzyme for ES uptake3 Publications
  2. Vmax=4.6 pmol/h/mg enzyme for PAH uptake3 Publications
  3. Vmax=0.9 pmol/h/mg enzyme for prostaglandin E2 uptake3 Publications
  4. Vmax=0.28 pmol/h/mg enzyme for ibuprofen uptake3 Publications
  5. Vmax=4.8 pmol/h/mg enzyme for ochratoxin A uptake3 Publications
  6. Vmax=5.1 pmol/h/mg enzyme for fluorescein uptake3 Publications
  7. Vmax=2.1 pmol/h/mg enzyme for 2-carboxyfluorescein-DM uptake3 Publications
  8. Vmax=3.9 pmol/h/mg enzyme for eosin-Y uptake3 Publications

GO - Molecular functioni

GO - Biological processi

  • organic anion transport Source: UniProtKB
  • sodium-independent organic anion transport Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Ion transport, Transport

Protein family/group databases

TCDBi2.A.1.19.16. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 22 member 20
Alternative name(s):
Organic anion transporter 6
Gene namesi
Name:Slc22a20
Synonyms:Gm963Imported, Oat6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2685809. Slc22a20.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1515CytoplasmicSequence analysisAdd
BLAST
Transmembranei16 – 3621HelicalSequence analysisAdd
BLAST
Topological domaini37 – 137101ExtracellularSequence analysisAdd
BLAST
Transmembranei138 – 15821HelicalSequence analysisAdd
BLAST
Topological domaini159 – 1668CytoplasmicSequence analysis
Transmembranei167 – 18721HelicalSequence analysisAdd
BLAST
Topological domaini188 – 1947ExtracellularSequence analysis
Transmembranei195 – 21521HelicalSequence analysisAdd
BLAST
Topological domaini216 – 22510CytoplasmicSequence analysis
Transmembranei226 – 24621HelicalSequence analysisAdd
BLAST
Topological domaini247 – 2504ExtracellularSequence analysis
Transmembranei251 – 27121HelicalSequence analysisAdd
BLAST
Topological domaini272 – 33968CytoplasmicSequence analysisAdd
BLAST
Transmembranei340 – 36021HelicalSequence analysisAdd
BLAST
Topological domaini361 – 3666ExtracellularSequence analysis
Transmembranei367 – 38721HelicalSequence analysisAdd
BLAST
Topological domaini388 – 39710CytoplasmicSequence analysis
Transmembranei398 – 41821HelicalSequence analysisAdd
BLAST
Topological domaini419 – 4257ExtracellularSequence analysis
Transmembranei426 – 44621HelicalSequence analysisAdd
BLAST
Topological domaini447 – 45711CytoplasmicSequence analysisAdd
BLAST
Transmembranei458 – 47821HelicalSequence analysisAdd
BLAST
Topological domaini479 – 4857ExtracellularSequence analysis
Transmembranei486 – 50621HelicalSequence analysisAdd
BLAST
Topological domaini507 – 55650CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL5269.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 556556Solute carrier family 22 member 20PRO_0000220512Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi39 – 391N-linked (GlcNAc...)Sequence analysis
Glycosylationi54 – 541N-linked (GlcNAc...)Sequence analysis
Glycosylationi61 – 611N-linked (GlcNAc...)Sequence analysis
Glycosylationi96 – 961N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ80UJ1.
PaxDbiQ80UJ1.
PRIDEiQ80UJ1.

PTM databases

iPTMnetiQ80UJ1.

Expressioni

Tissue specificityi

Highly expressed in olfactory mucosa. Weakly expressed in testis. Not detected in heart, spleen, lung, kidney or brain.2 Publications

Developmental stagei

Expressed at embryonic day 7, but not later in embryogenesis. Expressed in adult.1 Publication

Gene expression databases

BgeeiQ80UJ1.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000049473.

Chemistry

BindingDBiQ80UJ1.

Structurei

3D structure databases

ProteinModelPortaliQ80UJ1.
SMRiQ80UJ1. Positions 134-511.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0255. Eukaryota.
COG0477. LUCA.
GeneTreeiENSGT00760000118852.
HOGENOMiHOG000234569.
HOVERGENiHBG108433.
InParanoidiQ80UJ1.
KOiK08216.
OMAiYHAKARI.
OrthoDBiEOG7C8GH9.
PhylomeDBiQ80UJ1.
TreeFamiTF315847.

Family and domain databases

InterProiIPR020846. MFS_dom.
IPR005828. MFS_sugar_transport_like.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q80UJ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFTDLLDAL GGVGRFQLVY TALLLLPCGL LACHTFLQNF TAAAPPHHCQ
60 70 80 90 100
HPANYTEPTT NVSGVWLRAA IPLNQHGDPE PCRRYVEPQW ALLKPNASSH
110 120 130 140 150
GVATEGCKDG WVYDRSIFPS TIVMEWDLVC EARTLRDLAQ SIYMSGVLVG
160 170 180 190 200
AALFGGLADR LGRKAPLVWS YLQLAVSGAA TAYVGSFSAY CVFRFLMGMT
210 220 230 240 250
FSGIILNSLS LVVEWMPTRG RTVAGILLGF SFTLGQLILA GVAYLIRPWR
260 270 280 290 300
WLQFAVSAPF LVFFLYSWWL PESSRWLLLH GKAQQAVQNL QKVAMMNGRK
310 320 330 340 350
AEGERLTTEV VSSYIQDEFA SVRTSNSILD LFRTPAIRRV TCCLMGVWFS
360 370 380 390 400
NSVAYYGLAM DLQKFGLSIY LVQALFGIID IPAMLVATTT MIYVGRRATV
410 420 430 440 450
SSFLILAGLM VIANMFMPED LQTLRTVQAA LGKGCLASSF ICVYLFTGEL
460 470 480 490 500
YPTEIRQMGM GFASVNARLG GLVAPLITTL GEISPVLPPV SFGATSVLAG
510 520 530 540 550
MAVACFLTET RNVPLVETIA AMERRVKQGR SKRDTEQKSE EISLQQLGAS

PLKETI
Length:556
Mass (Da):60,944
Last modified:June 1, 2003 - v1
Checksum:iB0A89E00824AFC39
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY194118 mRNA. Translation: AAO91751.1.
BC046588 mRNA. Translation: AAH46588.1.
CCDSiCCDS29486.1.
RefSeqiNP_941052.1. NM_198650.2.
UniGeneiMm.329638.

Genome annotation databases

EnsembliENSMUST00000041827; ENSMUSP00000049473; ENSMUSG00000037451.
GeneIDi381203.
KEGGimmu:381203.
UCSCiuc008ggh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY194118 mRNA. Translation: AAO91751.1.
BC046588 mRNA. Translation: AAH46588.1.
CCDSiCCDS29486.1.
RefSeqiNP_941052.1. NM_198650.2.
UniGeneiMm.329638.

3D structure databases

ProteinModelPortaliQ80UJ1.
SMRiQ80UJ1. Positions 134-511.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000049473.

Chemistry

BindingDBiQ80UJ1.
ChEMBLiCHEMBL5269.

Protein family/group databases

TCDBi2.A.1.19.16. the major facilitator superfamily (mfs).

PTM databases

iPTMnetiQ80UJ1.

Proteomic databases

MaxQBiQ80UJ1.
PaxDbiQ80UJ1.
PRIDEiQ80UJ1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000041827; ENSMUSP00000049473; ENSMUSG00000037451.
GeneIDi381203.
KEGGimmu:381203.
UCSCiuc008ggh.1. mouse.

Organism-specific databases

CTDi440044.
MGIiMGI:2685809. Slc22a20.

Phylogenomic databases

eggNOGiKOG0255. Eukaryota.
COG0477. LUCA.
GeneTreeiENSGT00760000118852.
HOGENOMiHOG000234569.
HOVERGENiHBG108433.
InParanoidiQ80UJ1.
KOiK08216.
OMAiYHAKARI.
OrthoDBiEOG7C8GH9.
PhylomeDBiQ80UJ1.
TreeFamiTF315847.

Miscellaneous databases

PROiQ80UJ1.
SOURCEiSearch...

Gene expression databases

BgeeiQ80UJ1.

Family and domain databases

InterProiIPR020846. MFS_dom.
IPR005828. MFS_sugar_transport_like.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of murine OAT1-like organic anion transporter."
    Mount D.B.
    Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Olfactory epitheliumImported.
  3. "Identification of a novel murine organic anion transporter family member, OAT6, expressed in olfactory mucosa."
    Monte J.C., Nagle M.A., Eraly S.A., Nigam S.K.
    Biochem. Biophys. Res. Commun. 323:429-436(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PUTATIVE FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  4. "Transport of estrone sulfate by the novel organic anion transporter Oat6 (Slc22a20)."
    Schnabolk G.W., Youngblood G.L., Sweet D.H.
    Am. J. Physiol. 291:F314-F321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
  5. "Olfactory mucosa-expressed organic anion transporter, Oat6, manifests high affinity interactions with odorant organic anions."
    Kaler G., Truong D.M., Sweeney D.E., Logan D.W., Nagle M., Wu W., Eraly S.A., Nigam S.K.
    Biochem. Biophys. Res. Commun. 351:872-876(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, FUNCTION.
  6. "Structural variation governs substrate specificity for organic anion transporter (OAT) homologs. Potential remote sensing by OAT family members."
    Kaler G., Truong D.M., Khandelwal A., Nagle M., Eraly S.A., Swaan P.W., Nigam S.K.
    J. Biol. Chem. 282:23841-23853(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES.
  7. "Multi-level analysis of organic anion transporters 1, 3, and 6 reveals major differences in structural determinants of antiviral discrimination."
    Truong D.M., Kaler G., Khandelwal A., Swaan P.W., Nigam S.K.
    J. Biol. Chem. 283:8654-8663(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiS22AK_MOUSE
AccessioniPrimary (citable) accession number: Q80UJ1
Secondary accession number(s): Q5JBT1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: June 1, 2003
Last modified: July 6, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.