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Protein

Tubulin polyglutamylase TTLL4

Gene

Ttll4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Glutamylase which preferentially modifies beta-tubulin and non-tubulin proteins, such as NAP1L1, NAP1L4 and CGAS/MB21D1 (PubMed:17499049, PubMed:21074048, PubMed:20530212). Involved in the side-chain initiation step of the polyglutamylation reaction rather than in the elongation step (PubMed:17499049, PubMed:21074048). Involved in formation of short side-chains (PubMed:20530212). Mediates initiation of polyglutamylation of nucleosome assembly proteins NAP1L1 and NAP1L4 (PubMed:17499049). Also acts as a monoglutamylase: generates monoglutamylation of CGAS/MB21D1, leading to impair the nucleotidyltransferase activity of CGAS/MB21D1 (PubMed:26829768).4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei757ATPBy similarity1
Binding sitei759ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi744 – 747ATP bindingBy similarity4

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein-glutamic acid ligase activity Source: UniProtKB
  • tubulin binding Source: UniProtKB

GO - Biological processi

  • peptidyl-glutamic acid modification Source: UniProtKB
  • protein polyglutamylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tubulin polyglutamylase TTLL4 (EC:6.-.-.-)
Alternative name(s):
Tubulin--tyrosine ligase-like protein 4
Gene namesi
Name:Ttll4
Synonyms:Kiaa0173
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1914784. Ttll4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002124431 – 1193Tubulin polyglutamylase TTLL4Add BLAST1193

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei686PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ80UG8.
PRIDEiQ80UG8.

PTM databases

iPTMnetiQ80UG8.
PhosphoSitePlusiQ80UG8.

Expressioni

Tissue specificityi

Widely expressed with highest levels in testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000033257.
CleanExiMM_TTLL4.
ExpressionAtlasiQ80UG8. baseline and differential.
GenevisibleiQ80UG8. MM.

Interactioni

GO - Molecular functioni

  • tubulin binding Source: UniProtKB

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000037406.

Structurei

3D structure databases

ProteinModelPortaliQ80UG8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini599 – 942TTLPROSITE-ProRule annotationAdd BLAST344

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni913 – 1027c-MTBD regionBy similarityAdd BLAST115

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi522 – 527Poly-Glu6
Compositional biasi942 – 960Ser-richAdd BLAST19
Compositional biasi1079 – 1193Ser-richAdd BLAST115

Domaini

The flexible c-MTBD (cationic microtubule binding domain) region mediates binding to microtubules. It is positively charged and becomes ordered when bound to microtubules: it interacts with a negatively charged patch on tubulin. The presence of positive charges in the c-MTBD region is essential for proper binding.By similarity

Sequence similaritiesi

Belongs to the tubulin--tyrosine ligase family.Curated
Contains 1 TTL domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2156. Eukaryota.
ENOG410XTI2. LUCA.
GeneTreeiENSGT00760000118951.
HOGENOMiHOG000154712.
HOVERGENiHBG058942.
InParanoidiQ80UG8.
KOiK16601.
OMAiYKPMLNN.
OrthoDBiEOG091G03UM.
PhylomeDBiQ80UG8.
TreeFamiTF313087.

Family and domain databases

InterProiIPR004344. TTL/TTLL_fam.
IPR027748. TTLL-4.
[Graphical view]
PANTHERiPTHR12241:SF9. PTHR12241:SF9. 2 hits.
PfamiPF03133. TTL. 1 hit.
[Graphical view]
PROSITEiPS51221. TTL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q80UG8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASAGTEHYS IGLRRGNSFK QRHPSGTVSA SPSEKPSEVK VWSQAHQQVK
60 70 80 90 100
PIWKLEKKHV GTLSAGLGTS FLGVPSQPAY FLCPSTLCSS GTTAVIAGHS
110 120 130 140 150
NPCYLQSLPN LFSNTLLYRR TNVRQKPYQQ LESFCLRSSP SEKRSFSLPQ
160 170 180 190 200
KGLPVSVTAN KATSSTVFPM AQPMATSPTD PYLSLAAAGE NPSRKSLASA
210 220 230 240 250
ISGKIASPLS YKPMLNNNSF MRPNSTKVPL SQATDGLKPV SSPKIQPVSW
260 270 280 290 300
HHSGGTGDCV PQPGDHKVPQ NIATVLDDVT APITPSIPST LNISTASVTS
310 320 330 340 350
SQCSQSNFRM EAHPCGLDEN PDSQSATKEV HFTEAVRKLA EKGLEKMPRQ
360 370 380 390 400
GYQFEQACFV NPSFQWGLLN RSRRWKPLMG QRFPQEDIGL DSAILPGTSD
410 420 430 440 450
TLGLDSTVFC TKRISIHLLA SHVHGLNPSP ACGSAVDPQV LGEDRAPVPP
460 470 480 490 500
SSLQPLGVAE VATRLSSVHL DQPGKEPEEA KDLNSCTKGG GSATDLQPNQ
510 520 530 540 550
VEPEDTEDEL GDGLEDSCSH DENEEEEGDS ECSSLSVVSP SESVALISRN
560 570 580 590 600
CVDLMSKSLP NHEKVVRPAL IYSLFPNVTP TIYFGTRDER VEKLPWEQRR
610 620 630 640 650
LLRWKMSTVT PNIVKQTIGR SHFKISKRND DWLGCWGHHM KSPGFRSIRE
660 670 680 690 700
HQKLNHFPGS FQIGRKDRLW RNLSRMQSRF GKKEFSFFPQ SFILPQDSKL
710 720 730 740 750
LRKAWESSSR QKWIVKPPAS ARGIGIQVIH KWSQLPKRRP LLVQRYLHKP
760 770 780 790 800
YLISGSKFDL RIYVYVTSYD PLRIYLFSDG LVRFASCKYS PSMKSLSNKF
810 820 830 840 850
MHLTNYSVNK KNTEYQANAD ETACQGHKWA LKALWNYLSQ KGINSDAIWE
860 870 880 890 900
KIKDVVVKTI ISSEPYVTNL LKLYVRRPYS CHELFGFDIM LDENLKPWVL
910 920 930 940 950
EVNISPSLHS NSPLDISIKG QMIRDLLNLA GFVLPNMEDI ISSSSSPSSS
960 970 980 990 1000
SGSSTSLPSS PRDKCQMTPE HFTAQKMKKA YYLTQKIPDQ DFYASVLDVL
1010 1020 1030 1040 1050
TPDDVRVLVE MEDEFSRRGQ FERIFPSRIS SRYLRFFEQP RYFNILTTQW
1060 1070 1080 1090 1100
EQKYHGNKLK GVDLLRNWCY KGFHTGIVSD SAPLWSLPTS LMTTSKGDGT
1110 1120 1130 1140 1150
PNSASKSRKK SASEGTTLSS EDRSTPKSKK SQAGLSPISR KTLSSRSNEN
1160 1170 1180 1190
TSKQSKRSTP GLPVLKYSGQ SSRLSAASAS QSVTDSRLTA VSS
Length:1,193
Mass (Da):132,530
Last modified:January 10, 2006 - v3
Checksum:iE3C6FC5653D0E24E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM690747 mRNA. Translation: CAM84324.1.
BC044790 mRNA. Translation: AAH44790.1.
BC085151 mRNA. Translation: AAH85151.1.
AK129075 mRNA. Translation: BAC97885.1.
CCDSiCCDS15053.1.
RefSeqiNP_001014974.1. NM_001014974.1.
XP_006496287.1. XM_006496224.2.
XP_006496288.1. XM_006496225.2.
UniGeneiMm.260714.

Genome annotation databases

EnsembliENSMUST00000042125; ENSMUSP00000037406; ENSMUSG00000033257.
GeneIDi67534.
KEGGimmu:67534.
UCSCiuc007bmy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM690747 mRNA. Translation: CAM84324.1.
BC044790 mRNA. Translation: AAH44790.1.
BC085151 mRNA. Translation: AAH85151.1.
AK129075 mRNA. Translation: BAC97885.1.
CCDSiCCDS15053.1.
RefSeqiNP_001014974.1. NM_001014974.1.
XP_006496287.1. XM_006496224.2.
XP_006496288.1. XM_006496225.2.
UniGeneiMm.260714.

3D structure databases

ProteinModelPortaliQ80UG8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000037406.

PTM databases

iPTMnetiQ80UG8.
PhosphoSitePlusiQ80UG8.

Proteomic databases

PaxDbiQ80UG8.
PRIDEiQ80UG8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000042125; ENSMUSP00000037406; ENSMUSG00000033257.
GeneIDi67534.
KEGGimmu:67534.
UCSCiuc007bmy.1. mouse.

Organism-specific databases

CTDi9654.
MGIiMGI:1914784. Ttll4.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG2156. Eukaryota.
ENOG410XTI2. LUCA.
GeneTreeiENSGT00760000118951.
HOGENOMiHOG000154712.
HOVERGENiHBG058942.
InParanoidiQ80UG8.
KOiK16601.
OMAiYKPMLNN.
OrthoDBiEOG091G03UM.
PhylomeDBiQ80UG8.
TreeFamiTF313087.

Miscellaneous databases

ChiTaRSiTtll4. mouse.
PROiQ80UG8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033257.
CleanExiMM_TTLL4.
ExpressionAtlasiQ80UG8. baseline and differential.
GenevisibleiQ80UG8. MM.

Family and domain databases

InterProiIPR004344. TTL/TTLL_fam.
IPR027748. TTLL-4.
[Graphical view]
PANTHERiPTHR12241:SF9. PTHR12241:SF9. 2 hits.
PfamiPF03133. TTL. 1 hit.
[Graphical view]
PROSITEiPS51221. TTL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTTLL4_MOUSE
AccessioniPrimary (citable) accession number: Q80UG8
Secondary accession number(s): A4Q9E6, Q5U4C4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: January 10, 2006
Last modified: November 2, 2016
This is version 105 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.