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Q80UA9

- INSI2_RAT

UniProt

Q80UA9 - INSI2_RAT

Protein

Insulin-induced gene 2 protein

Gene

Insig2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Seems to regulate the ubiquitin-mediated proteasomal degradation of HMGCR By similarity.By similarity

    GO - Molecular functioni

    1. transcription factor binding Source: RGD

    GO - Biological processi

    1. cholesterol biosynthetic process Source: Ensembl
    2. cranial suture morphogenesis Source: Ensembl
    3. inner ear morphogenesis Source: Ensembl
    4. middle ear morphogenesis Source: Ensembl
    5. negative regulation of fatty acid biosynthetic process Source: Ensembl
    6. negative regulation of steroid biosynthetic process Source: Ensembl
    7. palate development Source: Ensembl
    8. response to fatty acid Source: RGD
    9. response to insulin Source: RGD
    10. response to lipid Source: RGD
    11. SREBP signaling pathway Source: Ensembl
    12. triglyceride metabolic process Source: Ensembl

    Keywords - Biological processi

    Cholesterol metabolism, Lipid metabolism, Steroid metabolism, Sterol metabolism

    Enzyme and pathway databases

    ReactomeiREACT_198603. Regulation of cholesterol biosynthesis by SREBP (SREBF).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Insulin-induced gene 2 protein
    Short name:
    INSIG-2
    Gene namesi
    Name:Insig2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 13

    Organism-specific databases

    RGDi631417. Insig2.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: RGD
    2. SREBP-SCAP-Insig complex Source: Ensembl

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 225225Insulin-induced gene 2 proteinPRO_0000286801Add
    BLAST

    Proteomic databases

    PRIDEiQ80UA9.

    PTM databases

    PhosphoSiteiQ80UA9.

    Expressioni

    Gene expression databases

    GenevestigatoriQ80UA9.

    Interactioni

    Subunit structurei

    Binds to the SCAP-SREBF2 complex only in the presence of sterols. Interacts with RNF139 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000003391.

    Structurei

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei26 – 4621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei71 – 9121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei131 – 14818HelicalSequence AnalysisAdd
    BLAST
    Transmembranei154 – 17421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei186 – 20621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the INSIG family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG263628.
    GeneTreeiENSGT00580000081600.
    HOGENOMiHOG000253021.
    HOVERGENiHBG058958.
    InParanoidiQ80UA9.
    OMAiHRFKREW.
    OrthoDBiEOG7RNK13.
    PhylomeDBiQ80UA9.
    TreeFamiTF331013.

    Family and domain databases

    InterProiIPR025929. INSIG_fam.
    [Graphical view]
    PfamiPF07281. INSIG. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q80UA9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEGETESPR PKKRGPYISS VTSQSVNVVI RGVVLFFIGV FLALVLNLLQ    50
    IQRNVTLFPP DVITSIFSSA WWVPPCCGTA SAVIGLLYPC IDRHLGEPHK 100
    FKREWSSVMR CVAVFVGINH ASAKVDFDNN FQFSLTLAAL SVGLWWTFDR 150
    SRSGFGLGVG IAFLATVVTQ LLVYNGVYQY TSPDFLYVRS WLPCIFFAGG 200
    ITMGNIGRQL AMYECKVIAE KSHQE 225
    Length:225
    Mass (Da):24,968
    Last modified:June 1, 2003 - v1
    Checksum:i534E933526CB2195
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY152392 mRNA. Translation: AAN78347.1.
    BC085682 mRNA. Translation: AAH85682.1.
    RefSeqiNP_835192.1. NM_178091.4.
    XP_006249729.1. XM_006249667.1.
    XP_006249730.1. XM_006249668.1.
    XP_006249731.1. XM_006249669.1.
    UniGeneiRn.16736.
    Rn.217040.

    Genome annotation databases

    EnsembliENSRNOT00000003391; ENSRNOP00000003391; ENSRNOG00000002478.
    GeneIDi288985.
    KEGGirno:288985.
    UCSCiRGD:631417. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY152392 mRNA. Translation: AAN78347.1 .
    BC085682 mRNA. Translation: AAH85682.1 .
    RefSeqi NP_835192.1. NM_178091.4.
    XP_006249729.1. XM_006249667.1.
    XP_006249730.1. XM_006249668.1.
    XP_006249731.1. XM_006249669.1.
    UniGenei Rn.16736.
    Rn.217040.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000003391.

    PTM databases

    PhosphoSitei Q80UA9.

    Proteomic databases

    PRIDEi Q80UA9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000003391 ; ENSRNOP00000003391 ; ENSRNOG00000002478 .
    GeneIDi 288985.
    KEGGi rno:288985.
    UCSCi RGD:631417. rat.

    Organism-specific databases

    CTDi 51141.
    RGDi 631417. Insig2.

    Phylogenomic databases

    eggNOGi NOG263628.
    GeneTreei ENSGT00580000081600.
    HOGENOMi HOG000253021.
    HOVERGENi HBG058958.
    InParanoidi Q80UA9.
    OMAi HRFKREW.
    OrthoDBi EOG7RNK13.
    PhylomeDBi Q80UA9.
    TreeFami TF331013.

    Enzyme and pathway databases

    Reactomei REACT_198603. Regulation of cholesterol biosynthesis by SREBP (SREBF).

    Miscellaneous databases

    NextBioi 629044.
    PROi Q80UA9.

    Gene expression databases

    Genevestigatori Q80UA9.

    Family and domain databases

    InterProi IPR025929. INSIG_fam.
    [Graphical view ]
    Pfami PF07281. INSIG. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Liver-specific mRNA for Insig-2 down-regulated by insulin: implications for fatty acid synthesis."
      Yabe D., Komuro R., Liang G., Goldstein J.L., Brown M.S.
      Proc. Natl. Acad. Sci. U.S.A. 100:3155-3160(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Heart.

    Entry informationi

    Entry nameiINSI2_RAT
    AccessioniPrimary (citable) accession number: Q80UA9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 15, 2007
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3