Q80UA9 (INSI2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Insulin-induced gene 2 protein
Short name=INSIG-2

Gene names

Name:

Insig2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Seems to regulate the ubiquitin-mediated proteasomal degradation of HMGCR By similarity.
Subunit structure	Binds to the SCAP-SREBF2 complex only in the presence of sterols. Interacts with RNF139 By similarity.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the INSIG family.

Ontologies

Keywords
Biological process	Cholesterol metabolism Lipid metabolism Steroid metabolism Sterol metabolism
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	SREBP signaling pathway Inferred from electronic annotation. Source: Compara cholesterol biosynthetic process Inferred from electronic annotation. Source: Compara cranial suture morphogenesis Inferred from electronic annotation. Source: Compara inner ear morphogenesis Inferred from electronic annotation. Source: Compara middle ear morphogenesis Inferred from electronic annotation. Source: Compara negative regulation of fatty acid biosynthetic process Inferred from electronic annotation. Source: Compara negative regulation of steroid biosynthetic process Inferred from electronic annotation. Source: Compara palate development Inferred from electronic annotation. Source: Compara response to fatty acid Inferred from expression pattern PubMed 16222032. Source: RGD response to insulin stimulus Inferred from expression pattern PubMed 16222032. Source: RGD triglyceride metabolic process Inferred from electronic annotation. Source: Compara
Cellular_component	SREBP-SCAP-Insig complex Inferred from electronic annotation. Source: Compara endoplasmic reticulum Inferred from direct assay Ref.1. Source: RGD
Molecular_function	transcription factor binding Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 225	225	Insulin-induced gene 2 protein		PRO_0000286801
Regions
	Transmembrane	26 – 46	21	Helical; Potential
	Transmembrane	71 – 91	21	Helical; Potential
	Transmembrane	131 – 148	18	Helical; Potential
	Transmembrane	154 – 174	21	Helical; Potential
	Transmembrane	186 – 206	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q80UA9 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 534E933526CB2195	FASTA	225	24,968
	10 20 30 40 50 60 MAEGETESPR PKKRGPYISS VTSQSVNVVI RGVVLFFIGV FLALVLNLLQ IQRNVTLFPP 70 80 90 100 110 120 DVITSIFSSA WWVPPCCGTA SAVIGLLYPC IDRHLGEPHK FKREWSSVMR CVAVFVGINH 130 140 150 160 170 180 ASAKVDFDNN FQFSLTLAAL SVGLWWTFDR SRSGFGLGVG IAFLATVVTQ LLVYNGVYQY 190 200 210 220 TSPDFLYVRS WLPCIFFAGG ITMGNIGRQL AMYECKVIAE KSHQE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Liver-specific mRNA for Insig-2 down-regulated by insulin: implications for fatty acid synthesis." Yabe D., Komuro R., Liang G., Goldstein J.L., Brown M.S. Proc. Natl. Acad. Sci. U.S.A. 100:3155-3160(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY152392 mRNA. Translation: AAN78347.1. BC085682 mRNA. Translation: AAH85682.1.
IPI	IPI00287193.
RefSeq	NP_835192.1. NM_178091.4.
UniGene	Rn.16736.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000003391.
PTM databases
PhosphoSite	Q80UA9.
Proteomic databases
PRIDE	Q80UA9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000003391; ENSRNOP00000003391; ENSRNOG00000002478.
GeneID	288985.
KEGG	rno:288985.
UCSC	RGD:631417. rat.
Organism-specific databases
CTD	51141.
RGD	631417. Insig2.
Phylogenomic databases
eggNOG	NOG263628.
GeneTree	ENSGT00580000081600.
HOGENOM	HOG000253021.
HOVERGEN	HBG058958.
InParanoid	Q80UA9.
OMA	QLALYEY.
OrthoDB	EOG4JDH7K.
Gene expression databases
Genevestigator	Q80UA9.
Family and domain databases
InterPro	IPR009904. INSIG. IPR025929. INSIG_fam. [Graphical view]
PANTHER	PTHR15301. PTHR15301. 1 hit.
Pfam	PF07281. INSIG. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	629044.

Entry information

Entry name

INSI2_RAT

Accession

Primary (citable) accession number: Q80UA9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	June 1, 2003
Last modified:	April 3, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program