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Protein

Insulin-induced gene 2 protein

Gene

Insig2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Seems to regulate the ubiquitin-mediated proteasomal degradation of HMGCR (By similarity).By similarity

GO - Molecular functioni

  1. transcription factor binding Source: RGD

GO - Biological processi

  1. cholesterol biosynthetic process Source: Ensembl
  2. cranial suture morphogenesis Source: Ensembl
  3. inner ear morphogenesis Source: Ensembl
  4. middle ear morphogenesis Source: Ensembl
  5. negative regulation of fatty acid biosynthetic process Source: Ensembl
  6. negative regulation of steroid biosynthetic process Source: Ensembl
  7. palate development Source: Ensembl
  8. response to fatty acid Source: RGD
  9. response to insulin Source: RGD
  10. response to lipid Source: RGD
  11. SREBP signaling pathway Source: Ensembl
  12. triglyceride metabolic process Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Cholesterol metabolism, Lipid metabolism, Steroid metabolism, Sterol metabolism

Enzyme and pathway databases

ReactomeiREACT_306909. Regulation of cholesterol biosynthesis by SREBP (SREBF).

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin-induced gene 2 protein
Short name:
INSIG-2
Gene namesi
Name:Insig2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi631417. Insig2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei26 – 4621HelicalSequence AnalysisAdd
BLAST
Transmembranei71 – 9121HelicalSequence AnalysisAdd
BLAST
Transmembranei131 – 14818HelicalSequence AnalysisAdd
BLAST
Transmembranei154 – 17421HelicalSequence AnalysisAdd
BLAST
Transmembranei186 – 20621HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: RGD
  2. SREBP-SCAP-Insig complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 225225Insulin-induced gene 2 proteinPRO_0000286801Add
BLAST

Proteomic databases

PRIDEiQ80UA9.

PTM databases

PhosphoSiteiQ80UA9.

Expressioni

Gene expression databases

GenevestigatoriQ80UA9.

Interactioni

Subunit structurei

Binds to the SCAP-SREBF2 complex only in the presence of sterols. Interacts with RNF139 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003391.

Family & Domainsi

Sequence similaritiesi

Belongs to the INSIG family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG263628.
GeneTreeiENSGT00580000081600.
HOGENOMiHOG000253021.
HOVERGENiHBG058958.
InParanoidiQ80UA9.
OMAiHRFKREW.
OrthoDBiEOG7RNK13.
PhylomeDBiQ80UA9.
TreeFamiTF331013.

Family and domain databases

InterProiIPR025929. INSIG_fam.
[Graphical view]
PfamiPF07281. INSIG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q80UA9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEGETESPR PKKRGPYISS VTSQSVNVVI RGVVLFFIGV FLALVLNLLQ
60 70 80 90 100
IQRNVTLFPP DVITSIFSSA WWVPPCCGTA SAVIGLLYPC IDRHLGEPHK
110 120 130 140 150
FKREWSSVMR CVAVFVGINH ASAKVDFDNN FQFSLTLAAL SVGLWWTFDR
160 170 180 190 200
SRSGFGLGVG IAFLATVVTQ LLVYNGVYQY TSPDFLYVRS WLPCIFFAGG
210 220
ITMGNIGRQL AMYECKVIAE KSHQE
Length:225
Mass (Da):24,968
Last modified:May 31, 2003 - v1
Checksum:i534E933526CB2195
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY152392 mRNA. Translation: AAN78347.1.
BC085682 mRNA. Translation: AAH85682.1.
RefSeqiNP_835192.1. NM_178091.4.
XP_006249729.1. XM_006249667.2.
XP_006249730.1. XM_006249668.2.
XP_006249731.1. XM_006249669.2.
UniGeneiRn.16736.
Rn.217040.

Genome annotation databases

EnsembliENSRNOT00000003391; ENSRNOP00000003391; ENSRNOG00000002478.
GeneIDi288985.
KEGGirno:288985.
UCSCiRGD:631417. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY152392 mRNA. Translation: AAN78347.1.
BC085682 mRNA. Translation: AAH85682.1.
RefSeqiNP_835192.1. NM_178091.4.
XP_006249729.1. XM_006249667.2.
XP_006249730.1. XM_006249668.2.
XP_006249731.1. XM_006249669.2.
UniGeneiRn.16736.
Rn.217040.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003391.

PTM databases

PhosphoSiteiQ80UA9.

Proteomic databases

PRIDEiQ80UA9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000003391; ENSRNOP00000003391; ENSRNOG00000002478.
GeneIDi288985.
KEGGirno:288985.
UCSCiRGD:631417. rat.

Organism-specific databases

CTDi51141.
RGDi631417. Insig2.

Phylogenomic databases

eggNOGiNOG263628.
GeneTreeiENSGT00580000081600.
HOGENOMiHOG000253021.
HOVERGENiHBG058958.
InParanoidiQ80UA9.
OMAiHRFKREW.
OrthoDBiEOG7RNK13.
PhylomeDBiQ80UA9.
TreeFamiTF331013.

Enzyme and pathway databases

ReactomeiREACT_306909. Regulation of cholesterol biosynthesis by SREBP (SREBF).

Miscellaneous databases

NextBioi629044.
PROiQ80UA9.

Gene expression databases

GenevestigatoriQ80UA9.

Family and domain databases

InterProiIPR025929. INSIG_fam.
[Graphical view]
PfamiPF07281. INSIG. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Liver-specific mRNA for Insig-2 down-regulated by insulin: implications for fatty acid synthesis."
    Yabe D., Komuro R., Liang G., Goldstein J.L., Brown M.S.
    Proc. Natl. Acad. Sci. U.S.A. 100:3155-3160(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.

Entry informationi

Entry nameiINSI2_RAT
AccessioniPrimary (citable) accession number: Q80UA9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 14, 2007
Last sequence update: May 31, 2003
Last modified: March 31, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.