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Protein

Nuclear pore complex protein Nup214

Gene

Nup214

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May serve as a docking site in the receptor-mediated import of substrates across the nuclear pore complex.By similarity

GO - Molecular functioni

GO - Biological processi

  • mRNA export from nucleus Source: MGI
  • protein export from nucleus Source: MGI
  • protein import into nucleus Source: MGI
  • regulation of cell cycle Source: MGI
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

ReactomeiR-MMU-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-MMU-170822. Regulation of Glucokinase by Glucokinase Regulatory Protein.
R-MMU-191859. snRNP Assembly.
R-MMU-3108214. SUMOylation of DNA damage response and repair proteins.
R-MMU-3301854. Nuclear Pore Complex (NPC) Disassembly.
R-MMU-3371453. Regulation of HSF1-mediated heat shock response.
R-MMU-450520. HuR (ELAVL1) binds and stabilizes mRNA.
R-MMU-4570464. SUMOylation of RNA binding proteins.
R-MMU-4615885. SUMOylation of DNA replication proteins.
R-MMU-5578749. Transcriptional regulation by small RNAs.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear pore complex protein Nup214
Alternative name(s):
214 kDa nucleoporin
Nucleoporin Nup214
Gene namesi
Name:Nup214
Synonyms:Kiaa0023
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1095411. Nup214.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003450142 – 2085Nuclear pore complex protein Nup214Add BLAST2084

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylglycineBy similarity1
Modified residuei30PhosphoserineBy similarity1
Modified residuei416PhosphothreonineBy similarity1
Modified residuei421PhosphoserineBy similarity1
Modified residuei430PhosphoserineBy similarity1
Modified residuei433PhosphoserineBy similarity1
Modified residuei437PhosphothreonineBy similarity1
Modified residuei645PhosphoserineBy similarity1
Modified residuei651PhosphoserineBy similarity1
Modified residuei660PhosphoserineBy similarity1
Modified residuei672PhosphoserineBy similarity1
Modified residuei754PhosphoserineBy similarity1
Modified residuei930PhosphoserineCombined sources1
Modified residuei960PhosphoserineBy similarity1
Modified residuei964PhosphoserineBy similarity1
Modified residuei979PhosphoserineBy similarity1
Modified residuei1027PhosphothreonineBy similarity1
Modified residuei1029PhosphoserineBy similarity1
Modified residuei1051PhosphoserineBy similarity1
Modified residuei1062PhosphoserineBy similarity1
Modified residuei1139PhosphoserineCombined sources1
Modified residuei1156PhosphothreonineBy similarity1
Modified residuei1162PhosphothreonineBy similarity1
Modified residuei1187PhosphoserineBy similarity1
Modified residuei1324PhosphothreonineBy similarity1
Modified residuei1365PhosphoserineBy similarity1
Modified residuei1980PhosphoserineBy similarity1

Post-translational modificationi

Probably glycosylated as it reacts with wheat germ agglutinin (WGA).By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ80U93.
MaxQBiQ80U93.
PaxDbiQ80U93.
PeptideAtlasiQ80U93.
PRIDEiQ80U93.

PTM databases

iPTMnetiQ80U93.
PhosphoSitePlusiQ80U93.
SwissPalmiQ80U93.

Expressioni

Gene expression databases

BgeeiENSMUSG00000001855.
ExpressionAtlasiQ80U93. baseline and differential.
GenevisibleiQ80U93. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with NUP214; this interaction increases upon lipopolysaccharide (LPS) stimulation. Interacts with DDX19. Interacts with NUP88. Interacts with XPO1. Interacts with XPO5.By similarity

Protein-protein interaction databases

BioGridi230673. 32 interactors.
IntActiQ80U93. 30 interactors.
STRINGi10090.ENSMUSP00000066492.

Structurei

3D structure databases

ProteinModelPortaliQ80U93.
SMRiQ80U93.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati41 – 93Blade 1By similarityAdd BLAST53
Repeati94 – 150Blade 2By similarityAdd BLAST57
Repeati151 – 193Blade 3By similarityAdd BLAST43
Repeati194 – 239Blade 4By similarityAdd BLAST46
Repeati240 – 303Blade 5By similarityAdd BLAST64
Repeati304 – 359Blade 6By similarityAdd BLAST56
Repeati360 – 404Blade 7By similarityAdd BLAST45

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni41 – 404Seven-bladed beta propellerBy similarityAdd BLAST364
Regioni479 – 207111 X 5 AA approximate repeatsAdd BLAST1593
Regioni734 – 762Leucine-zipper 1Add BLAST29
Regioni855 – 876Leucine-zipper 2Add BLAST22
Regioni1421 – 207918 X 4 AA approximate repeatsAdd BLAST659
Regioni1439 – 208011 X 3 AA approximate repeatsAdd BLAST642

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili674 – 1217By similarityAdd BLAST544

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1221 – 2085Pro/Ser/Thr-richAdd BLAST865

Domaini

Contains FG repeats.
The beta-propeller contains long interblade connector loops, and mediates interaction with DDX19B.By similarity

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG3630. Eukaryota.
ENOG410YBVS. LUCA.
GeneTreeiENSGT00730000111045.
HOGENOMiHOG000113878.
HOVERGENiHBG052683.
InParanoidiQ80U93.
KOiK14317.
OMAiFNFGIIT.
OrthoDBiEOG091G02Y1.
PhylomeDBiQ80U93.
TreeFamiTF323517.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
[Graphical view]
SMARTiSM00320. WD40. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q80U93-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDEMDAMIP EREMKDFQFR ALKKVRIFDS PEELPKERSS VLTISNKYGM
60 70 80 90 100
LFAGGTNGLN VFPTKSLLIQ NKPGDDPNKI VDTIQGLNVP MKFPVHHLAL
110 120 130 140 150
SCDSLTLSAC MMSSEYGSII AFFDVRTFSN QAKPLKRPFT YHKVSNDASG
160 170 180 190 200
MVNDMKWNPT VPSMVAVCLA DGSISVLQVT DVVKVCATLP PSTGVTCVCW
210 220 230 240 250
SPKGKQLAVG KQNGTVVQYL PTLQEKKVIP CPPFYESDHP VRVLDVLWIG
260 270 280 290 300
TYVFTIVYAG ADGTLETCPD VVMALLPKKE EKHPEIFVNF MEPCYSSCTE
310 320 330 340 350
RQHHYYLSYI EEWDLVLAAS AASTEVSILA RQNDQTNWES WLLEDSSRAE
360 370 380 390 400
LPVTDKSDDS LPMGVAIDYT NEVEVTINEE KTLPPAPVLL LLSTDGVLCP
410 420 430 440 450
FYMINQNPGV RSLIKTLELI STEGERQPKS SGSFPGTPSS PQAPQNLDAP
460 470 480 490 500
ATASSPLPPV SAAPTSTFPM PSAAGSPSVF SFGPSSFKSS ASVTGEPPLY
510 520 530 540 550
PTGSDSSRAA PGSGTSTFSF APPSKGSLAS TPAVAPVATS AAPFTFGFKP
560 570 580 590 600
TLESTPMSST PNTGMKPSFP PSASSVKVNL NEKFTAVASS APVHSSTSTP
610 620 630 640 650
SVLPFSSSPK PTASGPLSHP TPLPASSSSM PLKSSVSPSP AAGRSTQTAP
660 670 680 690 700
SSAPSTGQKS PRVNPPVPKS GSSQAKALQP PVTEKQRPQW KDSDPVLAGI
710 720 730 740 750
GEEIAHFQKE LEELKARTAK ACLQVGTSEE MKMLRTESDD LHTFLFEIRE
760 770 780 790 800
TTESLHGDIS TLKTTLLEGF AGVEEAREQH GRNHDSGYLH LLYKRPLDPK
810 820 830 840 850
SEAQLQEIRR LHQYVKFAVQ DVNDVLDLEW DRHLEQKKRQ RRLIVPERET
860 870 880 890 900
LFNTLANNRE IINQQRKRLN QLVDSLQQLR LYNHTAPWSL PSALSTQSNS
910 920 930 940 950
HSFDSDLECL LKTTIESHTK PSPRVPGKLS PAKQAQLRNF LAKRKTPPVR
960 970 980 990 1000
STAPASLSRS AFLSQRYYED LDEGSSASSV AQPLEGEDAR PTCTSVAQPL
1010 1020 1030 1040 1050
EGEDAQPICK EEEAVVPVPR HAPVVRTPSI QPSLLPQSMP FAKPHLIHSS
1060 1070 1080 1090 1100
SPAVMSSAVS TSATKVIPQG ADSTMLATKT VKHGAPGPSH TVAAPQAAAA
1110 1120 1130 1140 1150
AALRRQMASQ APAMSTLTES TLKTVPQVVN VQELRSNPSP PSAAMGSAVQ
1160 1170 1180 1190 1200
HSAAKTPHAV LTPVANSQAK QGSLINSFKP SGPTAASCQL SSGDKAVGQG
1210 1220 1230 1240 1250
TAKTESAATS TPSAAGQLNK PFSFASPGTF TFGTITPTPS SSFTATPGAG
1260 1270 1280 1290 1300
PPTKEPTQLE AFSFGGGGKP FSEAIPGNSP ATGATSAPST SVTAASLEDS
1310 1320 1330 1340 1350
APSSSKPAAP PETTVSSASS KLETPPSKLG ELLFPSSLAG ETLGSFSGLR
1360 1370 1380 1390 1400
VGQAEDSTKP VSKASSTNLA GAQPAKPSGV SFPNTSVLGK PVEPAVTSSV
1410 1420 1430 1440 1450
SPAPAAPASA LNVSTSSSSA TVFGSVPLTS AGPPGLISFG GAALASSKAS
1460 1470 1480 1490 1500
FSFGNQQTSS STASSATPTS TSVAPSLPAS FPTLSFGGLL SSPSASSLPV
1510 1520 1530 1540 1550
SSGKSTEEAA PPAVPDKSDS SEVSATTPSL PVQPQSTQAS LQTSDPVKKE
1560 1570 1580 1590 1600
PVLVQTTDSS PSRPASSASF VASTESMPVT LGAPDSKIEA VSPASTFAGP
1610 1620 1630 1640 1650
GQAAVATAVL PGAGSAATEA SGTPTTSTVS SSSPTSATET AVFGTATSGS
1660 1670 1680 1690 1700
SVFTQPPAAS SSSAFSQLSS NTATAPSATP VFGQVAASIT STAAATPQAS
1710 1720 1730 1740 1750
SSGFGSPAFG ASAPGVFGQT AFGQTPAFGQ ATSSPASGFS FSQPGFSSVP
1760 1770 1780 1790 1800
AFGQSVSSTP ASTSANVFGA TSSTSSPGSF SFGQASTNTG GTLFGQNNPP
1810 1820 1830 1840 1850
AFGQSPGFGQ GSSVFGGTSA TTSTAAPSGF SFCQASGFGS SNTGSVFGQA
1860 1870 1880 1890 1900
ANTGGSVFGQ SSTSSGGVFG SGNATRGGGF FSGLGGKPSQ DAANKNPFSS
1910 1920 1930 1940 1950
AGGGFGSTAA PNTSNLFGNS GAKTFGGFGS SSFGEQKPAG TFSSGGGSVA
1960 1970 1980 1990 2000
SQGFGFSTPN KTGGFGAAPV FGSPPTFGGS PGFGGVPAFG SAPAFTSPLG
2010 2020 2030 2040 2050
STGGKVFGEG TAAASAGGFG FGSSGNTASF GTLASQNAPT FGSLSQQTSG
2060 2070 2080
FGTPSSGFAG FGSGTGAFTF GSSNSSVQGF GGWRS
Length:2,085
Mass (Da):212,979
Last modified:July 22, 2008 - v2
Checksum:iC9998CC8BC27EE4A
GO

Sequence cautioni

The sequence BAC65471 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1973S → G in BAC65471 (PubMed:12693553).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122189 mRNA. Translation: BAC65471.1. Different initiation.
AL928893 Genomic DNA. Translation: CAM26045.1.
CCDSiCCDS38098.1.
RefSeqiNP_758472.2. NM_172268.2.
UniGeneiMm.289686.

Genome annotation databases

EnsembliENSMUST00000065398; ENSMUSP00000066492; ENSMUSG00000001855.
GeneIDi227720.
KEGGimmu:227720.
UCSCiuc008jeh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122189 mRNA. Translation: BAC65471.1. Different initiation.
AL928893 Genomic DNA. Translation: CAM26045.1.
CCDSiCCDS38098.1.
RefSeqiNP_758472.2. NM_172268.2.
UniGeneiMm.289686.

3D structure databases

ProteinModelPortaliQ80U93.
SMRiQ80U93.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230673. 32 interactors.
IntActiQ80U93. 30 interactors.
STRINGi10090.ENSMUSP00000066492.

PTM databases

iPTMnetiQ80U93.
PhosphoSitePlusiQ80U93.
SwissPalmiQ80U93.

Proteomic databases

EPDiQ80U93.
MaxQBiQ80U93.
PaxDbiQ80U93.
PeptideAtlasiQ80U93.
PRIDEiQ80U93.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000065398; ENSMUSP00000066492; ENSMUSG00000001855.
GeneIDi227720.
KEGGimmu:227720.
UCSCiuc008jeh.1. mouse.

Organism-specific databases

CTDi8021.
MGIiMGI:1095411. Nup214.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG3630. Eukaryota.
ENOG410YBVS. LUCA.
GeneTreeiENSGT00730000111045.
HOGENOMiHOG000113878.
HOVERGENiHBG052683.
InParanoidiQ80U93.
KOiK14317.
OMAiFNFGIIT.
OrthoDBiEOG091G02Y1.
PhylomeDBiQ80U93.
TreeFamiTF323517.

Enzyme and pathway databases

ReactomeiR-MMU-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-MMU-170822. Regulation of Glucokinase by Glucokinase Regulatory Protein.
R-MMU-191859. snRNP Assembly.
R-MMU-3108214. SUMOylation of DNA damage response and repair proteins.
R-MMU-3301854. Nuclear Pore Complex (NPC) Disassembly.
R-MMU-3371453. Regulation of HSF1-mediated heat shock response.
R-MMU-450520. HuR (ELAVL1) binds and stabilizes mRNA.
R-MMU-4570464. SUMOylation of RNA binding proteins.
R-MMU-4615885. SUMOylation of DNA replication proteins.
R-MMU-5578749. Transcriptional regulation by small RNAs.

Miscellaneous databases

ChiTaRSiNup214. mouse.
PROiQ80U93.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000001855.
ExpressionAtlasiQ80U93. baseline and differential.
GenevisibleiQ80U93. MM.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
[Graphical view]
SMARTiSM00320. WD40. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNU214_MOUSE
AccessioniPrimary (citable) accession number: Q80U93
Secondary accession number(s): A2ATN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: July 22, 2008
Last modified: November 30, 2016
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.