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Q80U93 (NU214_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear pore complex protein Nup214
Alternative name(s):
214 kDa nucleoporin
Nucleoporin Nup214
Gene names
Name:Nup214
Synonyms:Kiaa0023
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length2085 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May serve as a docking site in the receptor-mediated import of substrates across the nuclear pore complex By similarity.

Subunit structure

Homodimer. Interacts with DDX19, NUP88, XPO1 and XPO5 By similarity.

Subcellular location

Nucleusnuclear pore complex By similarity. Note: Cytoplasmic filaments By similarity.

Domain

Contains FG repeats.

The beta-propeller contains long interblade connector loops, and mediates interaction with DDX19B By similarity.

Post-translational modification

Probably glycosylated as it reacts with wheat germ agglutinin (WGA) By similarity.

Sequence caution

The sequence BAC65471.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 20852084Nuclear pore complex protein Nup214
PRO_0000345014

Regions

Repeat41 – 9353Blade 1 By similarity
Repeat94 – 15057Blade 2 By similarity
Repeat151 – 19343Blade 3 By similarity
Repeat194 – 23946Blade 4 By similarity
Repeat240 – 30364Blade 5 By similarity
Repeat304 – 35956Blade 6 By similarity
Repeat360 – 40445Blade 7 By similarity
Region41 – 404364Seven-bladed beta propeller By similarity
Region479 – 2071159311 X 5 AA approximate repeats
Region734 – 76229Leucine-zipper 1
Region855 – 87622Leucine-zipper 2
Region1421 – 207965918 X 4 AA approximate repeats
Region1439 – 208064211 X 3 AA approximate repeats
Coiled coil674 – 1217544 By similarity
Compositional bias1221 – 2085865Pro/Ser/Thr-rich

Amino acid modifications

Modified residue21N-acetylglycine By similarity
Modified residue4301Phosphoserine By similarity
Modified residue4331Phosphoserine By similarity
Modified residue4371Phosphothreonine By similarity
Modified residue6451Phosphoserine By similarity
Modified residue6511Phosphoserine By similarity
Modified residue6601Phosphoserine By similarity
Modified residue6721Phosphoserine By similarity
Modified residue9301Phosphoserine Ref.3
Modified residue9601Phosphoserine By similarity
Modified residue9641Phosphoserine By similarity
Modified residue9791Phosphoserine By similarity
Modified residue10291Phosphoserine By similarity
Modified residue10511Phosphoserine By similarity
Modified residue11391Phosphoserine Ref.3
Modified residue11561Phosphothreonine By similarity
Modified residue11621Phosphothreonine By similarity
Modified residue11871Phosphoserine By similarity
Modified residue13241Phosphothreonine By similarity
Modified residue19801Phosphoserine By similarity

Experimental info

Sequence conflict19731S → G in BAC65471. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q80U93 [UniParc].

Last modified July 22, 2008. Version 2.
Checksum: C9998CC8BC27EE4A

FASTA2,085212,979
        10         20         30         40         50         60 
MGDEMDAMIP EREMKDFQFR ALKKVRIFDS PEELPKERSS VLTISNKYGM LFAGGTNGLN 

        70         80         90        100        110        120 
VFPTKSLLIQ NKPGDDPNKI VDTIQGLNVP MKFPVHHLAL SCDSLTLSAC MMSSEYGSII 

       130        140        150        160        170        180 
AFFDVRTFSN QAKPLKRPFT YHKVSNDASG MVNDMKWNPT VPSMVAVCLA DGSISVLQVT 

       190        200        210        220        230        240 
DVVKVCATLP PSTGVTCVCW SPKGKQLAVG KQNGTVVQYL PTLQEKKVIP CPPFYESDHP 

       250        260        270        280        290        300 
VRVLDVLWIG TYVFTIVYAG ADGTLETCPD VVMALLPKKE EKHPEIFVNF MEPCYSSCTE 

       310        320        330        340        350        360 
RQHHYYLSYI EEWDLVLAAS AASTEVSILA RQNDQTNWES WLLEDSSRAE LPVTDKSDDS 

       370        380        390        400        410        420 
LPMGVAIDYT NEVEVTINEE KTLPPAPVLL LLSTDGVLCP FYMINQNPGV RSLIKTLELI 

       430        440        450        460        470        480 
STEGERQPKS SGSFPGTPSS PQAPQNLDAP ATASSPLPPV SAAPTSTFPM PSAAGSPSVF 

       490        500        510        520        530        540 
SFGPSSFKSS ASVTGEPPLY PTGSDSSRAA PGSGTSTFSF APPSKGSLAS TPAVAPVATS 

       550        560        570        580        590        600 
AAPFTFGFKP TLESTPMSST PNTGMKPSFP PSASSVKVNL NEKFTAVASS APVHSSTSTP 

       610        620        630        640        650        660 
SVLPFSSSPK PTASGPLSHP TPLPASSSSM PLKSSVSPSP AAGRSTQTAP SSAPSTGQKS 

       670        680        690        700        710        720 
PRVNPPVPKS GSSQAKALQP PVTEKQRPQW KDSDPVLAGI GEEIAHFQKE LEELKARTAK 

       730        740        750        760        770        780 
ACLQVGTSEE MKMLRTESDD LHTFLFEIRE TTESLHGDIS TLKTTLLEGF AGVEEAREQH 

       790        800        810        820        830        840 
GRNHDSGYLH LLYKRPLDPK SEAQLQEIRR LHQYVKFAVQ DVNDVLDLEW DRHLEQKKRQ 

       850        860        870        880        890        900 
RRLIVPERET LFNTLANNRE IINQQRKRLN QLVDSLQQLR LYNHTAPWSL PSALSTQSNS 

       910        920        930        940        950        960 
HSFDSDLECL LKTTIESHTK PSPRVPGKLS PAKQAQLRNF LAKRKTPPVR STAPASLSRS 

       970        980        990       1000       1010       1020 
AFLSQRYYED LDEGSSASSV AQPLEGEDAR PTCTSVAQPL EGEDAQPICK EEEAVVPVPR 

      1030       1040       1050       1060       1070       1080 
HAPVVRTPSI QPSLLPQSMP FAKPHLIHSS SPAVMSSAVS TSATKVIPQG ADSTMLATKT 

      1090       1100       1110       1120       1130       1140 
VKHGAPGPSH TVAAPQAAAA AALRRQMASQ APAMSTLTES TLKTVPQVVN VQELRSNPSP 

      1150       1160       1170       1180       1190       1200 
PSAAMGSAVQ HSAAKTPHAV LTPVANSQAK QGSLINSFKP SGPTAASCQL SSGDKAVGQG 

      1210       1220       1230       1240       1250       1260 
TAKTESAATS TPSAAGQLNK PFSFASPGTF TFGTITPTPS SSFTATPGAG PPTKEPTQLE 

      1270       1280       1290       1300       1310       1320 
AFSFGGGGKP FSEAIPGNSP ATGATSAPST SVTAASLEDS APSSSKPAAP PETTVSSASS 

      1330       1340       1350       1360       1370       1380 
KLETPPSKLG ELLFPSSLAG ETLGSFSGLR VGQAEDSTKP VSKASSTNLA GAQPAKPSGV 

      1390       1400       1410       1420       1430       1440 
SFPNTSVLGK PVEPAVTSSV SPAPAAPASA LNVSTSSSSA TVFGSVPLTS AGPPGLISFG 

      1450       1460       1470       1480       1490       1500 
GAALASSKAS FSFGNQQTSS STASSATPTS TSVAPSLPAS FPTLSFGGLL SSPSASSLPV 

      1510       1520       1530       1540       1550       1560 
SSGKSTEEAA PPAVPDKSDS SEVSATTPSL PVQPQSTQAS LQTSDPVKKE PVLVQTTDSS 

      1570       1580       1590       1600       1610       1620 
PSRPASSASF VASTESMPVT LGAPDSKIEA VSPASTFAGP GQAAVATAVL PGAGSAATEA 

      1630       1640       1650       1660       1670       1680 
SGTPTTSTVS SSSPTSATET AVFGTATSGS SVFTQPPAAS SSSAFSQLSS NTATAPSATP 

      1690       1700       1710       1720       1730       1740 
VFGQVAASIT STAAATPQAS SSGFGSPAFG ASAPGVFGQT AFGQTPAFGQ ATSSPASGFS 

      1750       1760       1770       1780       1790       1800 
FSQPGFSSVP AFGQSVSSTP ASTSANVFGA TSSTSSPGSF SFGQASTNTG GTLFGQNNPP 

      1810       1820       1830       1840       1850       1860 
AFGQSPGFGQ GSSVFGGTSA TTSTAAPSGF SFCQASGFGS SNTGSVFGQA ANTGGSVFGQ 

      1870       1880       1890       1900       1910       1920 
SSTSSGGVFG SGNATRGGGF FSGLGGKPSQ DAANKNPFSS AGGGFGSTAA PNTSNLFGNS 

      1930       1940       1950       1960       1970       1980 
GAKTFGGFGS SSFGEQKPAG TFSSGGGSVA SQGFGFSTPN KTGGFGAAPV FGSPPTFGGS 

      1990       2000       2010       2020       2030       2040 
PGFGGVPAFG SAPAFTSPLG STGGKVFGEG TAAASAGGFG FGSSGNTASF GTLASQNAPT 

      2050       2060       2070       2080 
FGSLSQQTSG FGTPSSGFAG FGSGTGAFTF GSSNSSVQGF GGWRS 

« Hide

References

[1]"Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-930 AND SER-1139, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK122189 mRNA. Translation: BAC65471.1. Different initiation.
AL928893 Genomic DNA. Translation: CAM26045.1.
CCDSCCDS38098.1.
RefSeqNP_758472.2. NM_172268.2.
UniGeneMm.289686.

3D structure databases

ProteinModelPortalQ80U93.
SMRQ80U93. Positions 1-433.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid230673. 11 interactions.
IntActQ80U93. 8 interactions.
STRING10090.ENSMUSP00000066492.

PTM databases

PhosphoSiteQ80U93.

Proteomic databases

MaxQBQ80U93.
PaxDbQ80U93.
PRIDEQ80U93.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000065398; ENSMUSP00000066492; ENSMUSG00000001855.
GeneID227720.
KEGGmmu:227720.
UCSCuc008jeh.1. mouse.

Organism-specific databases

CTD8021.
MGIMGI:1095411. Nup214.
RougeSearch...

Phylogenomic databases

eggNOGNOG12793.
GeneTreeENSGT00730000111045.
HOGENOMHOG000113878.
HOVERGENHBG052683.
InParanoidQ80U93.
KOK14317.
OMANERNRDS.
OrthoDBEOG7V765H.
PhylomeDBQ80U93.
TreeFamTF323517.

Gene expression databases

ArrayExpressQ80U93.
BgeeQ80U93.
GenevestigatorQ80U93.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
InterProIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
[Graphical view]
PfamPF00400. WD40. 1 hit.
[Graphical view]
SMARTSM00320. WD40. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNUP214. mouse.
NextBio378784.
PROQ80U93.
SOURCESearch...

Entry information

Entry nameNU214_MOUSE
AccessionPrimary (citable) accession number: Q80U93
Secondary accession number(s): A2ATN2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: July 22, 2008
Last modified: July 9, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot