Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q80U78 (PUM1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pumilio homolog 1
Gene names
Name:Pum1
Synonyms:Kiaa0099
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1189 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sequence-specific RNA-binding protein that negatively regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Capable of deadenylation-dependent and -independent modes of repression. May be required to support proliferation and self-renewal of stem cells By similarity.

Subunit structure

Recruits the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation By similarity.

Subcellular location

Cytoplasm Probable.

Tissue specificity

Widely expressed. Expressed in brain, heart, kidney, liver, lung, skin, intestine, spleen, testis and thymus. Weakly or not expressed in muscles and stomach. Expressed at various stages of myeloid and lymphoid cell development. Ref.1

Domain

The pumilio repeats mediate the association with RNA by packing together to form a right-handed superhelix that approximates a half doughnut. RNA-binding occurs on the concave side of the surface By similarity.

Sequence similarities

Contains 1 PUM-HD domain.

Contains 8 pumilio repeats.

Sequence caution

The sequence BAC65487.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processTranslation regulation
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
   DomainRepeat
   LigandRNA-binding
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processregulation of translation

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q80U78-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q80U78-2)

The sequence of this isoform differs from the canonical sequence as follows:
     418-418: Missing.
     952-953: Missing.
Isoform 3 (identifier: Q80U78-3)

The sequence of this isoform differs from the canonical sequence as follows:
     418-418: Missing.
Isoform 4 (identifier: Q80U78-4)

The sequence of this isoform differs from the canonical sequence as follows:
     181-189: DHSVSQPIM → GNLALASLL
     190-1189: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 11891188Pumilio homolog 1
PRO_0000075918

Regions

Domain829 – 1171343PUM-HD
Repeat849 – 88436Pumilio 1
Repeat885 – 92036Pumilio 2
Repeat921 – 95838Pumilio 3
Repeat959 – 99436Pumilio 4
Repeat995 – 103036Pumilio 5
Repeat1031 – 106636Pumilio 6
Repeat1067 – 110236Pumilio 7
Repeat1106 – 114540Pumilio 8
Compositional bias393 – 614222Ala-rich
Compositional bias476 – 52449Gln-rich
Compositional bias643 – 816174Ser-rich

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue191Phosphoserine By similarity
Modified residue751Phosphoserine By similarity
Modified residue981Phosphoserine By similarity
Modified residue1061Phosphoserine By similarity
Modified residue1241Phosphoserine Ref.5
Modified residue2091Phosphoserine By similarity
Modified residue7101Phosphoserine Ref.5 Ref.6
Modified residue8071Phosphoserine By similarity

Natural variations

Alternative sequence181 – 1899DHSVSQPIM → GNLALASLL in isoform 4.
VSP_009315
Alternative sequence190 – 11891000Missing in isoform 4.
VSP_009316
Alternative sequence4181Missing in isoform 2 and isoform 3.
VSP_009317
Alternative sequence952 – 9532Missing in isoform 2.
VSP_009318

Experimental info

Sequence conflict1861Q → H in AAG42319. Ref.1
Sequence conflict5451A → D in AAG42319. Ref.1
Sequence conflict5521P → T in AAG42319. Ref.1
Sequence conflict5551A → G in AAG42319. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 2, 2004. Version 2.
Checksum: CCB18634FBE3468A

FASTA1,189126,619
        10         20         30         40         50         60 
MSVACVLKRK AVLWQDSFSP HLKHHPQEPA NPNMPVVLTS GTGSQAQPQP AANQALAAGT 

        70         80         90        100        110        120 
HSSPVPGSIG VAGRSQDDAM VDYFFQRQHG EQLGGGGSGG GGYNTSKHRW PTGDNIHAEH 

       130        140        150        160        170        180 
QVRSMDELNH DFQALALEGR AMGEQLLPGK KFWETDESSK DGPKGIFLGD QWRDSAWGTS 

       190        200        210        220        230        240 
DHSVSQPIMV QRRPGQSFHV NSEVNSVLSP RSESGGLGVS MVEYVLSSSP GDSCLRKGGF 

       250        260        270        280        290        300 
GPRDADSDEN DKGEKKNKGT FDGDKLGDLK EEGDVMDKTN GLPVQNGIDA DVKDFSRTPG 

       310        320        330        340        350        360 
NCQNSANEVD LLGPNQNGSE GLAQLTSTNG AKPVEDFSNM ESQSVPLDPM EHVGMEPLQF 

       370        380        390        400        410        420 
DYSGTQVPVD SAAATVGLFD YNSQQQLFQR PNALAVQQLT AAQQQQYALA AAHQPHIAGL 

       430        440        450        460        470        480 
APAAFVPNPY IISAAPPGTD PYTAGLAAAA TLGPAVVPHQ YYGVTPWGVY PASLFQQQAA 

       490        500        510        520        530        540 
AAAAATNSAT QQSAPQAQQG QQQVLRGGAS QRPLTPNQNQ QGQQTDPLVA AAAVNSALAF 

       550        560        570        580        590        600 
GQGLAAGMPG YPVLAPAAYY DQTGALVVNA GARNGLGAPV RLVAPAPVII SSSAAQAAVA 

       610        620        630        640        650        660 
AAAASANGAA GGLAGTTNGP FRPLGTQQPQ PQPQQQPSNN LASSSFYGNN SLSSNSQSSS 

       670        680        690        700        710        720 
LFSQGSAQPA NTSLGFGSSS SLGATLGSAL GGFGTAVANS NTGSGSRRDS LTGSSDLYKR 

       730        740        750        760        770        780 
TSSSLAPIGH SFYSSLSYSS SPGPVGMPLP SQGPGHSQTP PPSLSSHGSS SSLNLGGLTN 

       790        800        810        820        830        840 
GSGRYISAAP GAEAKYRSAS SASSLFSPSS TLFSSSRLRY GMSDVMPSGR SRLLEDFRNN 

       850        860        870        880        890        900 
RYPNLQLREI AGHIMEFSQD QHGSRFIQLK LERATAAERQ LVFNEILQAA YQLMVDVFGN 

       910        920        930        940        950        960 
YVIQKFFEFG SHEQKLALAE RIRGHVLSLA LQMYGCRVIQ KALEFIPSDQ QVINEMVREL 

       970        980        990       1000       1010       1020 
DGHVLKCVKD QNGNHVVQKC IECVQPQSLQ FIIDAFKGQV FALSTHPYGC RVIQRILEHC 

      1030       1040       1050       1060       1070       1080 
LPDQTLPILE ELHQHTEQLV QDQYGNYVIQ HVLEHGRPED KSKIVAEIRG NVLVLSQHKF 

      1090       1100       1110       1120       1130       1140 
ASNVVEKCVT HASRTERAVL IDEVCTMNDG PHSALYTMMK DQYANYVVQK MIDVAEPGQR 

      1150       1160       1170       1180 
KIVMHKIRPH IATLRKYTYG KHILAKLEKY YMKNGVDLGP ICGPPNGII 

« Hide

Isoform 2 [UniParc].

Checksum: E3988AF20E34091A
Show »

FASTA1,186126,336
Isoform 3 [UniParc].

Checksum: 3959D5DC5825A160
Show »

FASTA1,188126,548
Isoform 4 [UniParc].

Checksum: 41749718B6FF8B1B
Show »

FASTA18920,206

References

« Hide 'large scale' references
[1]"Mouse Pum1 and Pum2 genes, members of the Pumilio family of RNA-binding proteins, show differential expression in fetal and adult hematopoietic stem cells and progenitors."
Spassov D.S., Jurecic R.
Blood Cells Mol. Dis. 30:55-69(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
Strain: C57BL/6J.
[2]"Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
Strain: C57BL/6J.
Tissue: Eye.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
Strain: C57BL/6.
Tissue: Brain.
[5]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-124 AND SER-710, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[6]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-710, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF321909 mRNA. Translation: AAG42319.1.
AK122205 mRNA. Translation: BAC65487.1. Different initiation.
AK052145 mRNA. Translation: BAC34857.1.
BC048174 mRNA. Translation: AAH48174.1.
BC050747 mRNA. Translation: AAH50747.1.
CCDSCCDS18710.1. [Q80U78-1]
CCDS51311.1. [Q80U78-2]
CCDS51312.1. [Q80U78-3]
RefSeqNP_001153075.1. NM_001159603.1. [Q80U78-2]
NP_001153076.1. NM_001159604.1. [Q80U78-3]
NP_001153077.1. NM_001159605.1.
NP_001153078.1. NM_001159606.1.
NP_109647.2. NM_030722.2. [Q80U78-1]
UniGeneMm.440206.

3D structure databases

ProteinModelPortalQ80U78.
SMRQ80U78. Positions 793-1171.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid219849. 1 interaction.
IntActQ80U78. 3 interactions.
MINTMINT-4109198.

PTM databases

PhosphoSiteQ80U78.

Proteomic databases

MaxQBQ80U78.
PaxDbQ80U78.
PRIDEQ80U78.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000030315; ENSMUSP00000030315; ENSMUSG00000028580. [Q80U78-1]
ENSMUST00000097862; ENSMUSP00000095474; ENSMUSG00000028580. [Q80U78-3]
ENSMUST00000097864; ENSMUSP00000095476; ENSMUSG00000028580. [Q80U78-2]
GeneID80912.
KEGGmmu:80912.
UCSCuc008uzl.2. mouse. [Q80U78-4]
uc008uzm.3. mouse. [Q80U78-1]

Organism-specific databases

CTD9698.
MGIMGI:1931749. Pum1.
RougeSearch...

Phylogenomic databases

eggNOGCOG5099.
GeneTreeENSGT00390000017241.
HOGENOMHOG000238461.
HOVERGENHBG049462.
InParanoidQ80U78.
KOK17943.
OMACLPEQTL.
OrthoDBEOG7T7GT5.
PhylomeDBQ80U78.
TreeFamTF318160.

Gene expression databases

ArrayExpressQ80U78.
BgeeQ80U78.
CleanExMM_PUM1.
GenevestigatorQ80U78.

Family and domain databases

Gene3D1.25.10.10. 1 hit.
InterProIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR001313. Pumilio_RNA-bd_rpt.
[Graphical view]
PfamPF00806. PUF. 8 hits.
[Graphical view]
SMARTSM00025. Pumilio. 8 hits.
[Graphical view]
SUPFAMSSF48371. SSF48371. 1 hit.
PROSITEPS50302. PUM. 8 hits.
PS50303. PUM_HD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPUM1. mouse.
NextBio350286.
PROQ80U78.
SOURCESearch...

Entry information

Entry namePUM1_MOUSE
AccessionPrimary (citable) accession number: Q80U78
Secondary accession number(s): Q80X96 expand/collapse secondary AC list , Q80YU8, Q8BPV7, Q9EPU6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: February 2, 2004
Last modified: July 9, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot