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Q80U78

- PUM1_MOUSE

UniProt

Q80U78 - PUM1_MOUSE

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Protein

Pumilio homolog 1

Gene

Pum1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Sequence-specific RNA-binding protein that negatively regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Capable of deadenylation-dependent and -independent modes of repression. May be required to support proliferation and self-renewal of stem cells (By similarity).By similarity

GO - Molecular functioni

  1. poly(A) RNA binding Source: Ensembl

GO - Biological processi

  1. regulation of translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Translation regulation

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiREACT_232671. Golgi Associated Vesicle Biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Pumilio homolog 1
Gene namesi
Name:Pum1
Synonyms:Kiaa0099
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:1931749. Pum1.

Subcellular locationi

Cytoplasm Curated

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 11891188Pumilio homolog 1PRO_0000075918Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei19 – 191PhosphoserineBy similarity
Modified residuei75 – 751PhosphoserineBy similarity
Modified residuei98 – 981PhosphoserineBy similarity
Modified residuei106 – 1061PhosphoserineBy similarity
Modified residuei124 – 1241Phosphoserine1 Publication
Modified residuei209 – 2091PhosphoserineBy similarity
Modified residuei710 – 7101Phosphoserine2 Publications
Modified residuei807 – 8071PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ80U78.
PaxDbiQ80U78.
PRIDEiQ80U78.

PTM databases

PhosphoSiteiQ80U78.

Expressioni

Tissue specificityi

Widely expressed. Expressed in brain, heart, kidney, liver, lung, skin, intestine, spleen, testis and thymus. Weakly or not expressed in muscles and stomach. Expressed at various stages of myeloid and lymphoid cell development.1 Publication

Gene expression databases

BgeeiQ80U78.
CleanExiMM_PUM1.
ExpressionAtlasiQ80U78. baseline and differential.
GenevestigatoriQ80U78.

Interactioni

Subunit structurei

Recruits the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation.By similarity

Protein-protein interaction databases

BioGridi219849. 1 interaction.
IntActiQ80U78. 3 interactions.
MINTiMINT-4109198.

Structurei

3D structure databases

ProteinModelPortaliQ80U78.
SMRiQ80U78. Positions 793-1171.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini829 – 1171343PUM-HDPROSITE-ProRule annotationAdd
BLAST
Repeati849 – 88436Pumilio 1Add
BLAST
Repeati885 – 92036Pumilio 2Add
BLAST
Repeati921 – 95838Pumilio 3Add
BLAST
Repeati959 – 99436Pumilio 4Add
BLAST
Repeati995 – 103036Pumilio 5Add
BLAST
Repeati1031 – 106636Pumilio 6Add
BLAST
Repeati1067 – 110236Pumilio 7Add
BLAST
Repeati1106 – 114540Pumilio 8Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi393 – 614222Ala-richAdd
BLAST
Compositional biasi476 – 52449Gln-richAdd
BLAST
Compositional biasi643 – 816174Ser-richAdd
BLAST

Domaini

The pumilio repeats mediate the association with RNA by packing together to form a right-handed superhelix that approximates a half doughnut. RNA-binding occurs on the concave side of the surface (By similarity).By similarity

Sequence similaritiesi

Contains 1 PUM-HD domain.PROSITE-ProRule annotation
Contains 8 pumilio repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5099.
GeneTreeiENSGT00390000017241.
HOGENOMiHOG000238461.
HOVERGENiHBG049462.
InParanoidiQ80U78.
KOiK17943.
OMAiCLPEQTL.
OrthoDBiEOG7T7GT5.
PhylomeDBiQ80U78.
TreeFamiTF318160.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR001313. Pumilio_RNA-bd_rpt.
[Graphical view]
PfamiPF00806. PUF. 8 hits.
[Graphical view]
SMARTiSM00025. Pumilio. 8 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50302. PUM. 8 hits.
PS50303. PUM_HD. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q80U78-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSVACVLKRK AVLWQDSFSP HLKHHPQEPA NPNMPVVLTS GTGSQAQPQP
60 70 80 90 100
AANQALAAGT HSSPVPGSIG VAGRSQDDAM VDYFFQRQHG EQLGGGGSGG
110 120 130 140 150
GGYNTSKHRW PTGDNIHAEH QVRSMDELNH DFQALALEGR AMGEQLLPGK
160 170 180 190 200
KFWETDESSK DGPKGIFLGD QWRDSAWGTS DHSVSQPIMV QRRPGQSFHV
210 220 230 240 250
NSEVNSVLSP RSESGGLGVS MVEYVLSSSP GDSCLRKGGF GPRDADSDEN
260 270 280 290 300
DKGEKKNKGT FDGDKLGDLK EEGDVMDKTN GLPVQNGIDA DVKDFSRTPG
310 320 330 340 350
NCQNSANEVD LLGPNQNGSE GLAQLTSTNG AKPVEDFSNM ESQSVPLDPM
360 370 380 390 400
EHVGMEPLQF DYSGTQVPVD SAAATVGLFD YNSQQQLFQR PNALAVQQLT
410 420 430 440 450
AAQQQQYALA AAHQPHIAGL APAAFVPNPY IISAAPPGTD PYTAGLAAAA
460 470 480 490 500
TLGPAVVPHQ YYGVTPWGVY PASLFQQQAA AAAAATNSAT QQSAPQAQQG
510 520 530 540 550
QQQVLRGGAS QRPLTPNQNQ QGQQTDPLVA AAAVNSALAF GQGLAAGMPG
560 570 580 590 600
YPVLAPAAYY DQTGALVVNA GARNGLGAPV RLVAPAPVII SSSAAQAAVA
610 620 630 640 650
AAAASANGAA GGLAGTTNGP FRPLGTQQPQ PQPQQQPSNN LASSSFYGNN
660 670 680 690 700
SLSSNSQSSS LFSQGSAQPA NTSLGFGSSS SLGATLGSAL GGFGTAVANS
710 720 730 740 750
NTGSGSRRDS LTGSSDLYKR TSSSLAPIGH SFYSSLSYSS SPGPVGMPLP
760 770 780 790 800
SQGPGHSQTP PPSLSSHGSS SSLNLGGLTN GSGRYISAAP GAEAKYRSAS
810 820 830 840 850
SASSLFSPSS TLFSSSRLRY GMSDVMPSGR SRLLEDFRNN RYPNLQLREI
860 870 880 890 900
AGHIMEFSQD QHGSRFIQLK LERATAAERQ LVFNEILQAA YQLMVDVFGN
910 920 930 940 950
YVIQKFFEFG SHEQKLALAE RIRGHVLSLA LQMYGCRVIQ KALEFIPSDQ
960 970 980 990 1000
QVINEMVREL DGHVLKCVKD QNGNHVVQKC IECVQPQSLQ FIIDAFKGQV
1010 1020 1030 1040 1050
FALSTHPYGC RVIQRILEHC LPDQTLPILE ELHQHTEQLV QDQYGNYVIQ
1060 1070 1080 1090 1100
HVLEHGRPED KSKIVAEIRG NVLVLSQHKF ASNVVEKCVT HASRTERAVL
1110 1120 1130 1140 1150
IDEVCTMNDG PHSALYTMMK DQYANYVVQK MIDVAEPGQR KIVMHKIRPH
1160 1170 1180
IATLRKYTYG KHILAKLEKY YMKNGVDLGP ICGPPNGII
Length:1,189
Mass (Da):126,619
Last modified:February 2, 2004 - v2
Checksum:iCCB18634FBE3468A
GO
Isoform 2 (identifier: Q80U78-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     418-418: Missing.
     952-953: Missing.

Show »
Length:1,186
Mass (Da):126,336
Checksum:iE3988AF20E34091A
GO
Isoform 3 (identifier: Q80U78-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     418-418: Missing.

Show »
Length:1,188
Mass (Da):126,548
Checksum:i3959D5DC5825A160
GO
Isoform 4 (identifier: Q80U78-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     181-189: DHSVSQPIM → GNLALASLL
     190-1189: Missing.

Note: No experimental confirmation available.

Show »
Length:189
Mass (Da):20,206
Checksum:i41749718B6FF8B1B
GO

Sequence cautioni

The sequence BAC65487.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti186 – 1861Q → H in AAG42319. (PubMed:12667987)Curated
Sequence conflicti545 – 5451A → D in AAG42319. (PubMed:12667987)Curated
Sequence conflicti552 – 5521P → T in AAG42319. (PubMed:12667987)Curated
Sequence conflicti555 – 5551A → G in AAG42319. (PubMed:12667987)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei181 – 1899DHSVSQPIM → GNLALASLL in isoform 4. 1 PublicationVSP_009315
Alternative sequencei190 – 11891000Missing in isoform 4. 1 PublicationVSP_009316Add
BLAST
Alternative sequencei418 – 4181Missing in isoform 2 and isoform 3. 1 PublicationVSP_009317
Alternative sequencei952 – 9532Missing in isoform 2. 1 PublicationVSP_009318

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF321909 mRNA. Translation: AAG42319.1.
AK122205 mRNA. Translation: BAC65487.1. Different initiation.
AK052145 mRNA. Translation: BAC34857.1.
BC048174 mRNA. Translation: AAH48174.1.
BC050747 mRNA. Translation: AAH50747.1.
CCDSiCCDS18710.1. [Q80U78-1]
CCDS51311.1. [Q80U78-2]
CCDS51312.1. [Q80U78-3]
RefSeqiNP_001153075.1. NM_001159603.1. [Q80U78-2]
NP_001153076.1. NM_001159604.1. [Q80U78-3]
NP_001153077.1. NM_001159605.1.
NP_001153078.1. NM_001159606.1.
NP_109647.2. NM_030722.2. [Q80U78-1]
UniGeneiMm.440206.

Genome annotation databases

EnsembliENSMUST00000030315; ENSMUSP00000030315; ENSMUSG00000028580. [Q80U78-1]
ENSMUST00000097862; ENSMUSP00000095474; ENSMUSG00000028580. [Q80U78-3]
ENSMUST00000097864; ENSMUSP00000095476; ENSMUSG00000028580. [Q80U78-2]
GeneIDi80912.
KEGGimmu:80912.
UCSCiuc008uzl.2. mouse. [Q80U78-4]
uc008uzm.3. mouse. [Q80U78-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF321909 mRNA. Translation: AAG42319.1 .
AK122205 mRNA. Translation: BAC65487.1 . Different initiation.
AK052145 mRNA. Translation: BAC34857.1 .
BC048174 mRNA. Translation: AAH48174.1 .
BC050747 mRNA. Translation: AAH50747.1 .
CCDSi CCDS18710.1. [Q80U78-1 ]
CCDS51311.1. [Q80U78-2 ]
CCDS51312.1. [Q80U78-3 ]
RefSeqi NP_001153075.1. NM_001159603.1. [Q80U78-2 ]
NP_001153076.1. NM_001159604.1. [Q80U78-3 ]
NP_001153077.1. NM_001159605.1.
NP_001153078.1. NM_001159606.1.
NP_109647.2. NM_030722.2. [Q80U78-1 ]
UniGenei Mm.440206.

3D structure databases

ProteinModelPortali Q80U78.
SMRi Q80U78. Positions 793-1171.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 219849. 1 interaction.
IntActi Q80U78. 3 interactions.
MINTi MINT-4109198.

PTM databases

PhosphoSitei Q80U78.

Proteomic databases

MaxQBi Q80U78.
PaxDbi Q80U78.
PRIDEi Q80U78.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000030315 ; ENSMUSP00000030315 ; ENSMUSG00000028580 . [Q80U78-1 ]
ENSMUST00000097862 ; ENSMUSP00000095474 ; ENSMUSG00000028580 . [Q80U78-3 ]
ENSMUST00000097864 ; ENSMUSP00000095476 ; ENSMUSG00000028580 . [Q80U78-2 ]
GeneIDi 80912.
KEGGi mmu:80912.
UCSCi uc008uzl.2. mouse. [Q80U78-4 ]
uc008uzm.3. mouse. [Q80U78-1 ]

Organism-specific databases

CTDi 9698.
MGIi MGI:1931749. Pum1.
Rougei Search...

Phylogenomic databases

eggNOGi COG5099.
GeneTreei ENSGT00390000017241.
HOGENOMi HOG000238461.
HOVERGENi HBG049462.
InParanoidi Q80U78.
KOi K17943.
OMAi CLPEQTL.
OrthoDBi EOG7T7GT5.
PhylomeDBi Q80U78.
TreeFami TF318160.

Enzyme and pathway databases

Reactomei REACT_232671. Golgi Associated Vesicle Biogenesis.

Miscellaneous databases

ChiTaRSi Pum1. mouse.
NextBioi 350286.
PROi Q80U78.
SOURCEi Search...

Gene expression databases

Bgeei Q80U78.
CleanExi MM_PUM1.
ExpressionAtlasi Q80U78. baseline and differential.
Genevestigatori Q80U78.

Family and domain databases

Gene3Di 1.25.10.10. 1 hit.
InterProi IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR001313. Pumilio_RNA-bd_rpt.
[Graphical view ]
Pfami PF00806. PUF. 8 hits.
[Graphical view ]
SMARTi SM00025. Pumilio. 8 hits.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 1 hit.
PROSITEi PS50302. PUM. 8 hits.
PS50303. PUM_HD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse Pum1 and Pum2 genes, members of the Pumilio family of RNA-binding proteins, show differential expression in fetal and adult hematopoietic stem cells and progenitors."
    Spassov D.S., Jurecic R.
    Blood Cells Mol. Dis. 30:55-69(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Strain: C57BL/6J.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
    DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
    Strain: C57BL/6J.
    Tissue: Eye.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
    Strain: C57BL/6.
    Tissue: Brain.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-124 AND SER-710, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-710, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPUM1_MOUSE
AccessioniPrimary (citable) accession number: Q80U78
Secondary accession number(s): Q80X96
, Q80YU8, Q8BPV7, Q9EPU6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: February 2, 2004
Last modified: November 26, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3