Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q80U78

- PUM1_MOUSE

UniProt

Q80U78 - PUM1_MOUSE

Protein

Pumilio homolog 1

Gene

Pum1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 2 (02 Feb 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Sequence-specific RNA-binding protein that negatively regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Capable of deadenylation-dependent and -independent modes of repression. May be required to support proliferation and self-renewal of stem cells By similarity.By similarity

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. regulation of translation Source: UniProtKB-KW

    Keywords - Biological processi

    Translation regulation

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pumilio homolog 1
    Gene namesi
    Name:Pum1
    Synonyms:Kiaa0099
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:1931749. Pum1.

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 11891188Pumilio homolog 1PRO_0000075918Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei19 – 191PhosphoserineBy similarity
    Modified residuei75 – 751PhosphoserineBy similarity
    Modified residuei98 – 981PhosphoserineBy similarity
    Modified residuei106 – 1061PhosphoserineBy similarity
    Modified residuei124 – 1241Phosphoserine1 Publication
    Modified residuei209 – 2091PhosphoserineBy similarity
    Modified residuei710 – 7101Phosphoserine2 Publications
    Modified residuei807 – 8071PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ80U78.
    PaxDbiQ80U78.
    PRIDEiQ80U78.

    PTM databases

    PhosphoSiteiQ80U78.

    Expressioni

    Tissue specificityi

    Widely expressed. Expressed in brain, heart, kidney, liver, lung, skin, intestine, spleen, testis and thymus. Weakly or not expressed in muscles and stomach. Expressed at various stages of myeloid and lymphoid cell development.1 Publication

    Gene expression databases

    ArrayExpressiQ80U78.
    BgeeiQ80U78.
    CleanExiMM_PUM1.
    GenevestigatoriQ80U78.

    Interactioni

    Subunit structurei

    Recruits the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation.By similarity

    Protein-protein interaction databases

    BioGridi219849. 1 interaction.
    IntActiQ80U78. 3 interactions.
    MINTiMINT-4109198.

    Structurei

    3D structure databases

    ProteinModelPortaliQ80U78.
    SMRiQ80U78. Positions 793-1171.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini829 – 1171343PUM-HDPROSITE-ProRule annotationAdd
    BLAST
    Repeati849 – 88436Pumilio 1Add
    BLAST
    Repeati885 – 92036Pumilio 2Add
    BLAST
    Repeati921 – 95838Pumilio 3Add
    BLAST
    Repeati959 – 99436Pumilio 4Add
    BLAST
    Repeati995 – 103036Pumilio 5Add
    BLAST
    Repeati1031 – 106636Pumilio 6Add
    BLAST
    Repeati1067 – 110236Pumilio 7Add
    BLAST
    Repeati1106 – 114540Pumilio 8Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi393 – 614222Ala-richAdd
    BLAST
    Compositional biasi476 – 52449Gln-richAdd
    BLAST
    Compositional biasi643 – 816174Ser-richAdd
    BLAST

    Domaini

    The pumilio repeats mediate the association with RNA by packing together to form a right-handed superhelix that approximates a half doughnut. RNA-binding occurs on the concave side of the surface By similarity.By similarity

    Sequence similaritiesi

    Contains 1 PUM-HD domain.PROSITE-ProRule annotation
    Contains 8 pumilio repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5099.
    GeneTreeiENSGT00390000017241.
    HOGENOMiHOG000238461.
    HOVERGENiHBG049462.
    InParanoidiQ80U78.
    KOiK17943.
    OMAiCLPEQTL.
    OrthoDBiEOG7T7GT5.
    PhylomeDBiQ80U78.
    TreeFamiTF318160.

    Family and domain databases

    Gene3Di1.25.10.10. 1 hit.
    InterProiIPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR001313. Pumilio_RNA-bd_rpt.
    [Graphical view]
    PfamiPF00806. PUF. 8 hits.
    [Graphical view]
    SMARTiSM00025. Pumilio. 8 hits.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 1 hit.
    PROSITEiPS50302. PUM. 8 hits.
    PS50303. PUM_HD. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q80U78-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSVACVLKRK AVLWQDSFSP HLKHHPQEPA NPNMPVVLTS GTGSQAQPQP     50
    AANQALAAGT HSSPVPGSIG VAGRSQDDAM VDYFFQRQHG EQLGGGGSGG 100
    GGYNTSKHRW PTGDNIHAEH QVRSMDELNH DFQALALEGR AMGEQLLPGK 150
    KFWETDESSK DGPKGIFLGD QWRDSAWGTS DHSVSQPIMV QRRPGQSFHV 200
    NSEVNSVLSP RSESGGLGVS MVEYVLSSSP GDSCLRKGGF GPRDADSDEN 250
    DKGEKKNKGT FDGDKLGDLK EEGDVMDKTN GLPVQNGIDA DVKDFSRTPG 300
    NCQNSANEVD LLGPNQNGSE GLAQLTSTNG AKPVEDFSNM ESQSVPLDPM 350
    EHVGMEPLQF DYSGTQVPVD SAAATVGLFD YNSQQQLFQR PNALAVQQLT 400
    AAQQQQYALA AAHQPHIAGL APAAFVPNPY IISAAPPGTD PYTAGLAAAA 450
    TLGPAVVPHQ YYGVTPWGVY PASLFQQQAA AAAAATNSAT QQSAPQAQQG 500
    QQQVLRGGAS QRPLTPNQNQ QGQQTDPLVA AAAVNSALAF GQGLAAGMPG 550
    YPVLAPAAYY DQTGALVVNA GARNGLGAPV RLVAPAPVII SSSAAQAAVA 600
    AAAASANGAA GGLAGTTNGP FRPLGTQQPQ PQPQQQPSNN LASSSFYGNN 650
    SLSSNSQSSS LFSQGSAQPA NTSLGFGSSS SLGATLGSAL GGFGTAVANS 700
    NTGSGSRRDS LTGSSDLYKR TSSSLAPIGH SFYSSLSYSS SPGPVGMPLP 750
    SQGPGHSQTP PPSLSSHGSS SSLNLGGLTN GSGRYISAAP GAEAKYRSAS 800
    SASSLFSPSS TLFSSSRLRY GMSDVMPSGR SRLLEDFRNN RYPNLQLREI 850
    AGHIMEFSQD QHGSRFIQLK LERATAAERQ LVFNEILQAA YQLMVDVFGN 900
    YVIQKFFEFG SHEQKLALAE RIRGHVLSLA LQMYGCRVIQ KALEFIPSDQ 950
    QVINEMVREL DGHVLKCVKD QNGNHVVQKC IECVQPQSLQ FIIDAFKGQV 1000
    FALSTHPYGC RVIQRILEHC LPDQTLPILE ELHQHTEQLV QDQYGNYVIQ 1050
    HVLEHGRPED KSKIVAEIRG NVLVLSQHKF ASNVVEKCVT HASRTERAVL 1100
    IDEVCTMNDG PHSALYTMMK DQYANYVVQK MIDVAEPGQR KIVMHKIRPH 1150
    IATLRKYTYG KHILAKLEKY YMKNGVDLGP ICGPPNGII 1189
    Length:1,189
    Mass (Da):126,619
    Last modified:February 2, 2004 - v2
    Checksum:iCCB18634FBE3468A
    GO
    Isoform 2 (identifier: Q80U78-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         418-418: Missing.
         952-953: Missing.

    Show »
    Length:1,186
    Mass (Da):126,336
    Checksum:iE3988AF20E34091A
    GO
    Isoform 3 (identifier: Q80U78-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         418-418: Missing.

    Show »
    Length:1,188
    Mass (Da):126,548
    Checksum:i3959D5DC5825A160
    GO
    Isoform 4 (identifier: Q80U78-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         181-189: DHSVSQPIM → GNLALASLL
         190-1189: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:189
    Mass (Da):20,206
    Checksum:i41749718B6FF8B1B
    GO

    Sequence cautioni

    The sequence BAC65487.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti186 – 1861Q → H in AAG42319. (PubMed:12667987)Curated
    Sequence conflicti545 – 5451A → D in AAG42319. (PubMed:12667987)Curated
    Sequence conflicti552 – 5521P → T in AAG42319. (PubMed:12667987)Curated
    Sequence conflicti555 – 5551A → G in AAG42319. (PubMed:12667987)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei181 – 1899DHSVSQPIM → GNLALASLL in isoform 4. 1 PublicationVSP_009315
    Alternative sequencei190 – 11891000Missing in isoform 4. 1 PublicationVSP_009316Add
    BLAST
    Alternative sequencei418 – 4181Missing in isoform 2 and isoform 3. 1 PublicationVSP_009317
    Alternative sequencei952 – 9532Missing in isoform 2. 1 PublicationVSP_009318

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF321909 mRNA. Translation: AAG42319.1.
    AK122205 mRNA. Translation: BAC65487.1. Different initiation.
    AK052145 mRNA. Translation: BAC34857.1.
    BC048174 mRNA. Translation: AAH48174.1.
    BC050747 mRNA. Translation: AAH50747.1.
    CCDSiCCDS18710.1. [Q80U78-1]
    CCDS51311.1. [Q80U78-2]
    CCDS51312.1. [Q80U78-3]
    RefSeqiNP_001153075.1. NM_001159603.1. [Q80U78-2]
    NP_001153076.1. NM_001159604.1. [Q80U78-3]
    NP_001153077.1. NM_001159605.1.
    NP_001153078.1. NM_001159606.1.
    NP_109647.2. NM_030722.2. [Q80U78-1]
    UniGeneiMm.440206.

    Genome annotation databases

    EnsembliENSMUST00000030315; ENSMUSP00000030315; ENSMUSG00000028580. [Q80U78-1]
    ENSMUST00000097862; ENSMUSP00000095474; ENSMUSG00000028580. [Q80U78-3]
    ENSMUST00000097864; ENSMUSP00000095476; ENSMUSG00000028580. [Q80U78-2]
    GeneIDi80912.
    KEGGimmu:80912.
    UCSCiuc008uzl.2. mouse. [Q80U78-4]
    uc008uzm.3. mouse. [Q80U78-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF321909 mRNA. Translation: AAG42319.1 .
    AK122205 mRNA. Translation: BAC65487.1 . Different initiation.
    AK052145 mRNA. Translation: BAC34857.1 .
    BC048174 mRNA. Translation: AAH48174.1 .
    BC050747 mRNA. Translation: AAH50747.1 .
    CCDSi CCDS18710.1. [Q80U78-1 ]
    CCDS51311.1. [Q80U78-2 ]
    CCDS51312.1. [Q80U78-3 ]
    RefSeqi NP_001153075.1. NM_001159603.1. [Q80U78-2 ]
    NP_001153076.1. NM_001159604.1. [Q80U78-3 ]
    NP_001153077.1. NM_001159605.1.
    NP_001153078.1. NM_001159606.1.
    NP_109647.2. NM_030722.2. [Q80U78-1 ]
    UniGenei Mm.440206.

    3D structure databases

    ProteinModelPortali Q80U78.
    SMRi Q80U78. Positions 793-1171.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 219849. 1 interaction.
    IntActi Q80U78. 3 interactions.
    MINTi MINT-4109198.

    PTM databases

    PhosphoSitei Q80U78.

    Proteomic databases

    MaxQBi Q80U78.
    PaxDbi Q80U78.
    PRIDEi Q80U78.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000030315 ; ENSMUSP00000030315 ; ENSMUSG00000028580 . [Q80U78-1 ]
    ENSMUST00000097862 ; ENSMUSP00000095474 ; ENSMUSG00000028580 . [Q80U78-3 ]
    ENSMUST00000097864 ; ENSMUSP00000095476 ; ENSMUSG00000028580 . [Q80U78-2 ]
    GeneIDi 80912.
    KEGGi mmu:80912.
    UCSCi uc008uzl.2. mouse. [Q80U78-4 ]
    uc008uzm.3. mouse. [Q80U78-1 ]

    Organism-specific databases

    CTDi 9698.
    MGIi MGI:1931749. Pum1.
    Rougei Search...

    Phylogenomic databases

    eggNOGi COG5099.
    GeneTreei ENSGT00390000017241.
    HOGENOMi HOG000238461.
    HOVERGENi HBG049462.
    InParanoidi Q80U78.
    KOi K17943.
    OMAi CLPEQTL.
    OrthoDBi EOG7T7GT5.
    PhylomeDBi Q80U78.
    TreeFami TF318160.

    Miscellaneous databases

    ChiTaRSi PUM1. mouse.
    NextBioi 350286.
    PROi Q80U78.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q80U78.
    Bgeei Q80U78.
    CleanExi MM_PUM1.
    Genevestigatori Q80U78.

    Family and domain databases

    Gene3Di 1.25.10.10. 1 hit.
    InterProi IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR001313. Pumilio_RNA-bd_rpt.
    [Graphical view ]
    Pfami PF00806. PUF. 8 hits.
    [Graphical view ]
    SMARTi SM00025. Pumilio. 8 hits.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 1 hit.
    PROSITEi PS50302. PUM. 8 hits.
    PS50303. PUM_HD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse Pum1 and Pum2 genes, members of the Pumilio family of RNA-binding proteins, show differential expression in fetal and adult hematopoietic stem cells and progenitors."
      Spassov D.S., Jurecic R.
      Blood Cells Mol. Dis. 30:55-69(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
      Strain: C57BL/6J.
    2. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
      DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
      Strain: C57BL/6J.
      Tissue: Eye.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
      Strain: C57BL/6.
      Tissue: Brain.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-124 AND SER-710, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    6. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-710, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPUM1_MOUSE
    AccessioniPrimary (citable) accession number: Q80U78
    Secondary accession number(s): Q80X96
    , Q80YU8, Q8BPV7, Q9EPU6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 2, 2004
    Last sequence update: February 2, 2004
    Last modified: October 1, 2014
    This is version 95 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3