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Q80U78

- PUM1_MOUSE

UniProt

Q80U78 - PUM1_MOUSE

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Protein

Pumilio homolog 1

Gene
Pum1, Kiaa0099
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Sequence-specific RNA-binding protein that negatively regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Capable of deadenylation-dependent and -independent modes of repression. May be required to support proliferation and self-renewal of stem cells By similarity.

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. regulation of translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Translation regulation

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pumilio homolog 1
Gene namesi
Name:Pum1
Synonyms:Kiaa0099
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:1931749. Pum1.

Subcellular locationi

Cytoplasm Inferred

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 11891188Pumilio homolog 1PRO_0000075918Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine By similarity
Modified residuei19 – 191Phosphoserine By similarity
Modified residuei75 – 751Phosphoserine By similarity
Modified residuei98 – 981Phosphoserine By similarity
Modified residuei106 – 1061Phosphoserine By similarity
Modified residuei124 – 1241Phosphoserine1 Publication
Modified residuei209 – 2091Phosphoserine By similarity
Modified residuei710 – 7101Phosphoserine2 Publications
Modified residuei807 – 8071Phosphoserine By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ80U78.
PaxDbiQ80U78.
PRIDEiQ80U78.

PTM databases

PhosphoSiteiQ80U78.

Expressioni

Tissue specificityi

Widely expressed. Expressed in brain, heart, kidney, liver, lung, skin, intestine, spleen, testis and thymus. Weakly or not expressed in muscles and stomach. Expressed at various stages of myeloid and lymphoid cell development.1 Publication

Gene expression databases

ArrayExpressiQ80U78.
BgeeiQ80U78.
CleanExiMM_PUM1.
GenevestigatoriQ80U78.

Interactioni

Subunit structurei

Recruits the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation By similarity.

Protein-protein interaction databases

BioGridi219849. 1 interaction.
IntActiQ80U78. 3 interactions.
MINTiMINT-4109198.

Structurei

3D structure databases

ProteinModelPortaliQ80U78.
SMRiQ80U78. Positions 793-1171.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini829 – 1171343PUM-HDAdd
BLAST
Repeati849 – 88436Pumilio 1Add
BLAST
Repeati885 – 92036Pumilio 2Add
BLAST
Repeati921 – 95838Pumilio 3Add
BLAST
Repeati959 – 99436Pumilio 4Add
BLAST
Repeati995 – 103036Pumilio 5Add
BLAST
Repeati1031 – 106636Pumilio 6Add
BLAST
Repeati1067 – 110236Pumilio 7Add
BLAST
Repeati1106 – 114540Pumilio 8Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi393 – 614222Ala-richAdd
BLAST
Compositional biasi476 – 52449Gln-richAdd
BLAST
Compositional biasi643 – 816174Ser-richAdd
BLAST

Domaini

The pumilio repeats mediate the association with RNA by packing together to form a right-handed superhelix that approximates a half doughnut. RNA-binding occurs on the concave side of the surface By similarity.

Sequence similaritiesi

Contains 1 PUM-HD domain.
Contains 8 pumilio repeats.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5099.
GeneTreeiENSGT00390000017241.
HOGENOMiHOG000238461.
HOVERGENiHBG049462.
InParanoidiQ80U78.
KOiK17943.
OMAiCLPEQTL.
OrthoDBiEOG7T7GT5.
PhylomeDBiQ80U78.
TreeFamiTF318160.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR001313. Pumilio_RNA-bd_rpt.
[Graphical view]
PfamiPF00806. PUF. 8 hits.
[Graphical view]
SMARTiSM00025. Pumilio. 8 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50302. PUM. 8 hits.
PS50303. PUM_HD. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q80U78-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSVACVLKRK AVLWQDSFSP HLKHHPQEPA NPNMPVVLTS GTGSQAQPQP     50
AANQALAAGT HSSPVPGSIG VAGRSQDDAM VDYFFQRQHG EQLGGGGSGG 100
GGYNTSKHRW PTGDNIHAEH QVRSMDELNH DFQALALEGR AMGEQLLPGK 150
KFWETDESSK DGPKGIFLGD QWRDSAWGTS DHSVSQPIMV QRRPGQSFHV 200
NSEVNSVLSP RSESGGLGVS MVEYVLSSSP GDSCLRKGGF GPRDADSDEN 250
DKGEKKNKGT FDGDKLGDLK EEGDVMDKTN GLPVQNGIDA DVKDFSRTPG 300
NCQNSANEVD LLGPNQNGSE GLAQLTSTNG AKPVEDFSNM ESQSVPLDPM 350
EHVGMEPLQF DYSGTQVPVD SAAATVGLFD YNSQQQLFQR PNALAVQQLT 400
AAQQQQYALA AAHQPHIAGL APAAFVPNPY IISAAPPGTD PYTAGLAAAA 450
TLGPAVVPHQ YYGVTPWGVY PASLFQQQAA AAAAATNSAT QQSAPQAQQG 500
QQQVLRGGAS QRPLTPNQNQ QGQQTDPLVA AAAVNSALAF GQGLAAGMPG 550
YPVLAPAAYY DQTGALVVNA GARNGLGAPV RLVAPAPVII SSSAAQAAVA 600
AAAASANGAA GGLAGTTNGP FRPLGTQQPQ PQPQQQPSNN LASSSFYGNN 650
SLSSNSQSSS LFSQGSAQPA NTSLGFGSSS SLGATLGSAL GGFGTAVANS 700
NTGSGSRRDS LTGSSDLYKR TSSSLAPIGH SFYSSLSYSS SPGPVGMPLP 750
SQGPGHSQTP PPSLSSHGSS SSLNLGGLTN GSGRYISAAP GAEAKYRSAS 800
SASSLFSPSS TLFSSSRLRY GMSDVMPSGR SRLLEDFRNN RYPNLQLREI 850
AGHIMEFSQD QHGSRFIQLK LERATAAERQ LVFNEILQAA YQLMVDVFGN 900
YVIQKFFEFG SHEQKLALAE RIRGHVLSLA LQMYGCRVIQ KALEFIPSDQ 950
QVINEMVREL DGHVLKCVKD QNGNHVVQKC IECVQPQSLQ FIIDAFKGQV 1000
FALSTHPYGC RVIQRILEHC LPDQTLPILE ELHQHTEQLV QDQYGNYVIQ 1050
HVLEHGRPED KSKIVAEIRG NVLVLSQHKF ASNVVEKCVT HASRTERAVL 1100
IDEVCTMNDG PHSALYTMMK DQYANYVVQK MIDVAEPGQR KIVMHKIRPH 1150
IATLRKYTYG KHILAKLEKY YMKNGVDLGP ICGPPNGII 1189
Length:1,189
Mass (Da):126,619
Last modified:February 2, 2004 - v2
Checksum:iCCB18634FBE3468A
GO
Isoform 2 (identifier: Q80U78-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     418-418: Missing.
     952-953: Missing.

Show »
Length:1,186
Mass (Da):126,336
Checksum:iE3988AF20E34091A
GO
Isoform 3 (identifier: Q80U78-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     418-418: Missing.

Show »
Length:1,188
Mass (Da):126,548
Checksum:i3959D5DC5825A160
GO
Isoform 4 (identifier: Q80U78-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     181-189: DHSVSQPIM → GNLALASLL
     190-1189: Missing.

Note: No experimental confirmation available.

Show »
Length:189
Mass (Da):20,206
Checksum:i41749718B6FF8B1B
GO

Sequence cautioni

The sequence BAC65487.1 differs from that shown. Reason: Erroneous initiation.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei181 – 1899DHSVSQPIM → GNLALASLL in isoform 4. VSP_009315
Alternative sequencei190 – 11891000Missing in isoform 4. VSP_009316Add
BLAST
Alternative sequencei418 – 4181Missing in isoform 2 and isoform 3. VSP_009317
Alternative sequencei952 – 9532Missing in isoform 2. VSP_009318

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti186 – 1861Q → H in AAG42319. 1 Publication
Sequence conflicti545 – 5451A → D in AAG42319. 1 Publication
Sequence conflicti552 – 5521P → T in AAG42319. 1 Publication
Sequence conflicti555 – 5551A → G in AAG42319. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF321909 mRNA. Translation: AAG42319.1.
AK122205 mRNA. Translation: BAC65487.1. Different initiation.
AK052145 mRNA. Translation: BAC34857.1.
BC048174 mRNA. Translation: AAH48174.1.
BC050747 mRNA. Translation: AAH50747.1.
CCDSiCCDS18710.1. [Q80U78-1]
CCDS51311.1. [Q80U78-2]
CCDS51312.1. [Q80U78-3]
RefSeqiNP_001153075.1. NM_001159603.1. [Q80U78-2]
NP_001153076.1. NM_001159604.1. [Q80U78-3]
NP_001153077.1. NM_001159605.1.
NP_001153078.1. NM_001159606.1.
NP_109647.2. NM_030722.2. [Q80U78-1]
UniGeneiMm.440206.

Genome annotation databases

EnsembliENSMUST00000030315; ENSMUSP00000030315; ENSMUSG00000028580. [Q80U78-1]
ENSMUST00000097862; ENSMUSP00000095474; ENSMUSG00000028580. [Q80U78-3]
ENSMUST00000097864; ENSMUSP00000095476; ENSMUSG00000028580. [Q80U78-2]
GeneIDi80912.
KEGGimmu:80912.
UCSCiuc008uzl.2. mouse. [Q80U78-4]
uc008uzm.3. mouse. [Q80U78-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF321909 mRNA. Translation: AAG42319.1 .
AK122205 mRNA. Translation: BAC65487.1 . Different initiation.
AK052145 mRNA. Translation: BAC34857.1 .
BC048174 mRNA. Translation: AAH48174.1 .
BC050747 mRNA. Translation: AAH50747.1 .
CCDSi CCDS18710.1. [Q80U78-1 ]
CCDS51311.1. [Q80U78-2 ]
CCDS51312.1. [Q80U78-3 ]
RefSeqi NP_001153075.1. NM_001159603.1. [Q80U78-2 ]
NP_001153076.1. NM_001159604.1. [Q80U78-3 ]
NP_001153077.1. NM_001159605.1.
NP_001153078.1. NM_001159606.1.
NP_109647.2. NM_030722.2. [Q80U78-1 ]
UniGenei Mm.440206.

3D structure databases

ProteinModelPortali Q80U78.
SMRi Q80U78. Positions 793-1171.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 219849. 1 interaction.
IntActi Q80U78. 3 interactions.
MINTi MINT-4109198.

PTM databases

PhosphoSitei Q80U78.

Proteomic databases

MaxQBi Q80U78.
PaxDbi Q80U78.
PRIDEi Q80U78.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000030315 ; ENSMUSP00000030315 ; ENSMUSG00000028580 . [Q80U78-1 ]
ENSMUST00000097862 ; ENSMUSP00000095474 ; ENSMUSG00000028580 . [Q80U78-3 ]
ENSMUST00000097864 ; ENSMUSP00000095476 ; ENSMUSG00000028580 . [Q80U78-2 ]
GeneIDi 80912.
KEGGi mmu:80912.
UCSCi uc008uzl.2. mouse. [Q80U78-4 ]
uc008uzm.3. mouse. [Q80U78-1 ]

Organism-specific databases

CTDi 9698.
MGIi MGI:1931749. Pum1.
Rougei Search...

Phylogenomic databases

eggNOGi COG5099.
GeneTreei ENSGT00390000017241.
HOGENOMi HOG000238461.
HOVERGENi HBG049462.
InParanoidi Q80U78.
KOi K17943.
OMAi CLPEQTL.
OrthoDBi EOG7T7GT5.
PhylomeDBi Q80U78.
TreeFami TF318160.

Miscellaneous databases

ChiTaRSi PUM1. mouse.
NextBioi 350286.
PROi Q80U78.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q80U78.
Bgeei Q80U78.
CleanExi MM_PUM1.
Genevestigatori Q80U78.

Family and domain databases

Gene3Di 1.25.10.10. 1 hit.
InterProi IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR001313. Pumilio_RNA-bd_rpt.
[Graphical view ]
Pfami PF00806. PUF. 8 hits.
[Graphical view ]
SMARTi SM00025. Pumilio. 8 hits.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 1 hit.
PROSITEi PS50302. PUM. 8 hits.
PS50303. PUM_HD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse Pum1 and Pum2 genes, members of the Pumilio family of RNA-binding proteins, show differential expression in fetal and adult hematopoietic stem cells and progenitors."
    Spassov D.S., Jurecic R.
    Blood Cells Mol. Dis. 30:55-69(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Strain: C57BL/6J.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
    DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
    Strain: C57BL/6J.
    Tissue: Eye.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
    Strain: C57BL/6.
    Tissue: Brain.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-124 AND SER-710, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-710, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPUM1_MOUSE
AccessioniPrimary (citable) accession number: Q80U78
Secondary accession number(s): Q80X96
, Q80YU8, Q8BPV7, Q9EPU6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: February 2, 2004
Last modified: July 9, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi