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Protein

Polycomb protein Suz12

Gene

Suz12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (By similarity). Genes repressed by the PRC2/EED-EZH2 complex include HOXA7, HOXB6 and HOXC8.By similarity3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri450 – 47324C2H2-typeAdd
BLAST

GO - Molecular functioni

  • chromatin binding Source: MGI
  • chromatin DNA binding Source: MGI
  • histone methyltransferase activity Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • methylated histone binding Source: MGI
  • RNA binding Source: MGI
  • RNA polymerase II core promoter sequence-specific DNA binding Source: MGI
  • sequence-specific DNA binding Source: MGI

GO - Biological processi

  • histone methylation Source: MGI
  • histone ubiquitination Source: MGI
  • negative regulation of cell differentiation Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of cell proliferation Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-212300. PRC2 methylates histones and DNA.
R-MMU-2559580. Oxidative Stress Induced Senescence.
R-MMU-3214841. PKMTs methylate histone lysines.
R-MMU-5617472. Activation of anterior HOX genes in hindbrain development during early embryogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Polycomb protein Suz12
Alternative name(s):
Suppressor of zeste 12 protein homolog
Gene namesi
Name:Suz12
Synonyms:D11Ertd530e, Kiaa0160
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1261758. Suz12.

Subcellular locationi

GO - Cellular componenti

  • ESC/E(Z) complex Source: UniProtKB
  • nucleolus Source: MGI
  • nucleoplasm Source: Reactome
  • nucleus Source: MGI
  • protein-DNA complex Source: MGI
  • sex chromatin Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice exhibit early embryonic lethality and defects in gastrulation accompanied by reduced methylation of 'Lys-27' of histone H3.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 741741Polycomb protein Suz12PRO_0000047058Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki74 – 74Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki75 – 75Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki77 – 77Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei548 – 5481PhosphoserineCombined sources
Modified residuei585 – 5851PhosphoserineCombined sources

Post-translational modificationi

Sumoylated, probably by PIAS2.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ80U70.
MaxQBiQ80U70.
PaxDbiQ80U70.
PRIDEiQ80U70.

PTM databases

iPTMnetiQ80U70.
PhosphoSiteiQ80U70.

Expressioni

Developmental stagei

Expression increases in prostate during prostate tumor development.1 Publication

Gene expression databases

BgeeiQ80U70.
CleanExiMM_SUZ12.
ExpressionAtlasiQ80U70. baseline and differential.
GenevisibleiQ80U70. MM.

Interactioni

Subunit structurei

Interacts with histone H1 and PHF19 (By similarity). Interacts with CDYL (By similarity). Component of the PRC2/EED-EZH2 complex, which includes EED, EZH2, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. The minimum components required for methyltransferase activity of the PRC2/EED-EZH2 complex are EED, EZH2 and SUZ12. Component of the PRC2/EED-EZH1 complex, which includes EED, EZH1, SUZ12, RBBP4 and AEBP2. The PRC2 complex may also interact with DNMT1, DNMT3A, DNMT3B and PHF1 via the EZH2 subunit and with SIRT1 via the SUZ12 subunit. Interacts with WDR77. Interacts with ARNTL/BMAL1.By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Ezh2Q6118810EBI-2526494,EBI-904311
Ezh2Q6AXH73EBI-2526494,EBI-6876582
Jarid2Q6231512EBI-2526494,EBI-493592
Kdm5aQ3UXZ92EBI-2526494,EBI-2531441

GO - Molecular functioni

Protein-protein interaction databases

BioGridi206694. 16 interactions.
DIPiDIP-56045N.
IntActiQ80U70. 17 interactions.
MINTiMINT-4136170.
STRINGi10090.ENSMUSP00000017692.

Structurei

3D structure databases

ProteinModelPortaliQ80U70.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni565 – 64177VEFS-boxAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi7 – 5044Gly-richAdd
BLAST
Compositional biasi29 – 6941Ala-richAdd
BLAST

Sequence similaritiesi

Contains 1 C2H2-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri450 – 47324C2H2-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2350. Eukaryota.
ENOG410YJ29. LUCA.
GeneTreeiENSGT00390000012364.
HOGENOMiHOG000047340.
HOVERGENiHBG039522.
InParanoidiQ80U70.
KOiK11463.
OMAiETYVAQM.
OrthoDBiEOG7DC23W.
PhylomeDBiQ80U70.
TreeFamiTF323249.

Family and domain databases

InterProiIPR019135. Polycomb_protein_VEFS-Box.
[Graphical view]
PfamiPF09733. VEFS-Box. 1 hit.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q80U70-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPQKHGGGG GGGSGPSAGS GGGGFGGSAA AVAAAASGGK SGGGGCGGGG
60 70 80 90 100
SYSASSSSAA AAAAAAGAAV LPVKKPKMEH VQADHELFLQ AFEKPTQIYR
110 120 130 140 150
FLRTRNLIAP IFLHRTLTYM SHRNSRTSIK RKTFKVDDML SKVEKMKGEQ
160 170 180 190 200
ESHSLSAHLQ LTFTGFFHKN DKPSQNSENE QNSVTLEVLL VKVCHKKRKD
210 220 230 240 250
VSCPIRQVPT GKKQVPLNPD LNQTKPGNFP SLAVSSNEFE PSNSHMVKSY
260 270 280 290 300
SLLFRVTRPG RREFNGMING ETNENIDVSE ELPARRKRNR EDGEKTFVAQ
310 320 330 340 350
MTVFDKNRRL QLLDGEYEVA MQEMEECPIS KKRATWETIL DGKRLPPFET
360 370 380 390 400
FSQGPTLQFT LRWTGETNDK STAPVAKPLA TRNSESLHQE NKPGSVKPAQ
410 420 430 440 450
TIAVKETLTT ELQTRKEKDN SNESRQKLRI FYQFLYNNNT RQQTEARDDL
460 470 480 490 500
HCPWCTLNCR KLYSLLKHLK LCHSRFIFNY VYHPKGARID VSINECYDGS
510 520 530 540 550
YAGNPQDIHR QPGFAFSRNG PVKRTPITHI LVCRPKRTKA SMSEFLESED
560 570 580 590 600
GEVEQQRTYS SGHNRLYFHS DTCLPLRPQE MEVDSEDEKD PEWLREKTIT
610 620 630 640 650
QIEEFSDVNE GEKEVMKLWN LHVMKHGFIA DNQMNHACML FVENYGQKII
660 670 680 690 700
KKNLCRNFML HLVSMHDFNL ISIMSIDKAV TKLREMQQKL EKGESATPSN
710 720 730 740
EEIAEEQNGT ANGFSETNSK EKALETDGVS GVPKQSKKQK L
Length:741
Mass (Da):83,026
Last modified:July 11, 2003 - v2
Checksum:i4A6B6E52D24FAEF2
GO

Sequence cautioni

The sequence BAC65495.1 differs from that shown.Unknown reasons.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122213 Transcribed RNA. Translation: BAC65495.1. Sequence problems.
AL591113 Genomic DNA. Translation: CAI24761.1.
BC003922 mRNA. Translation: AAH03922.1.
BC051099 mRNA. Translation: AAH51099.1.
BC064461 mRNA. Translation: AAH64461.1.
CCDSiCCDS25125.1.
RefSeqiNP_001156490.1. NM_001163018.1.
NP_954666.1. NM_199196.2.
UniGeneiMm.283410.
Mm.473315.

Genome annotation databases

EnsembliENSMUST00000017692; ENSMUSP00000017692; ENSMUSG00000017548.
GeneIDi52615.
KEGGimmu:52615.
UCSCiuc007klc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122213 Transcribed RNA. Translation: BAC65495.1. Sequence problems.
AL591113 Genomic DNA. Translation: CAI24761.1.
BC003922 mRNA. Translation: AAH03922.1.
BC051099 mRNA. Translation: AAH51099.1.
BC064461 mRNA. Translation: AAH64461.1.
CCDSiCCDS25125.1.
RefSeqiNP_001156490.1. NM_001163018.1.
NP_954666.1. NM_199196.2.
UniGeneiMm.283410.
Mm.473315.

3D structure databases

ProteinModelPortaliQ80U70.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206694. 16 interactions.
DIPiDIP-56045N.
IntActiQ80U70. 17 interactions.
MINTiMINT-4136170.
STRINGi10090.ENSMUSP00000017692.

PTM databases

iPTMnetiQ80U70.
PhosphoSiteiQ80U70.

Proteomic databases

EPDiQ80U70.
MaxQBiQ80U70.
PaxDbiQ80U70.
PRIDEiQ80U70.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000017692; ENSMUSP00000017692; ENSMUSG00000017548.
GeneIDi52615.
KEGGimmu:52615.
UCSCiuc007klc.1. mouse.

Organism-specific databases

CTDi23512.
MGIiMGI:1261758. Suz12.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG2350. Eukaryota.
ENOG410YJ29. LUCA.
GeneTreeiENSGT00390000012364.
HOGENOMiHOG000047340.
HOVERGENiHBG039522.
InParanoidiQ80U70.
KOiK11463.
OMAiETYVAQM.
OrthoDBiEOG7DC23W.
PhylomeDBiQ80U70.
TreeFamiTF323249.

Enzyme and pathway databases

ReactomeiR-MMU-212300. PRC2 methylates histones and DNA.
R-MMU-2559580. Oxidative Stress Induced Senescence.
R-MMU-3214841. PKMTs methylate histone lysines.
R-MMU-5617472. Activation of anterior HOX genes in hindbrain development during early embryogenesis.

Miscellaneous databases

PROiQ80U70.
SOURCEiSearch...

Gene expression databases

BgeeiQ80U70.
CleanExiMM_SUZ12.
ExpressionAtlasiQ80U70. baseline and differential.
GenevisibleiQ80U70. MM.

Family and domain databases

InterProiIPR019135. Polycomb_protein_VEFS-Box.
[Graphical view]
PfamiPF09733. VEFS-Box. 1 hit.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
    DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Brain and Kidney.
  4. "Suz12 is essential for mouse development and for EZH2 histone methyltransferase activity."
    Pasini D., Bracken A.P., Jensen M.R., Lazzerini Denchi E., Helin K.
    EMBO J. 23:4061-4071(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  5. "Imprinting along the Kcnq1 domain on mouse chromosome 7 involves repressive histone methylation and recruitment of Polycomb group complexes."
    Umlauf D., Goto Y., Cao R., Cerqueira F., Wagschal A., Zhang Y., Feil R.
    Nat. Genet. 36:1296-1300(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Composition and histone substrates of polycomb repressive group complexes change during cellular differentiation."
    Kuzmichev A., Margueron R., Vaquero A., Preissner T.S., Scher M., Kirmizis A., Ouyang X., Brockdorff N., Abate-Shen C., Farnham P.J., Reinberg D.
    Proc. Natl. Acad. Sci. U.S.A. 102:1859-1864(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  7. "Association of Polycomb group SUZ12 with WD-repeat protein MEP50 that binds to histone H2A selectively in vitro."
    Furuno K., Masatsugu T., Sonoda M., Sasazuki T., Yamamoto K.
    Biochem. Biophys. Res. Commun. 345:1051-1058(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH WDR77.
  8. "The Polycomb group protein Eed protects the inactive X-chromosome from differentiation-induced reactivation."
    Kalantry S., Mills K.C., Yee D., Otte A.P., Panning B., Magnuson T.
    Nat. Cell Biol. 8:195-202(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  9. "The polycomb group protein Suz12 is required for embryonic stem cell differentiation."
    Pasini D., Bracken A.P., Hansen J.B., Capillo M., Helin K.
    Mol. Cell. Biol. 27:3769-3779(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  10. "EZH1 mediates methylation on histone H3 lysine 27 and complements EZH2 in maintaining stem cell identity and executing pluripotency."
    Shen X., Liu Y., Hsu Y.-J., Fujiwara Y., Kim J., Mao X., Yuan G.-C., Orkin S.H.
    Mol. Cell 32:491-502(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE PRC2/EED-EZH1 COMPLEX.
  11. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-548 AND SER-585, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney, Liver, Lung, Spleen and Testis.
  13. "Polycomb-like 2 associates with PRC2 and regulates transcriptional networks during mouse embryonic stem cell self-renewal and differentiation."
    Walker E., Chang W.Y., Hunkapiller J., Cagney G., Garcha K., Torchia J., Krogan N.J., Reiter J.F., Stanford W.L.
    Cell Stem Cell 6:153-166(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE PRC2 COMPLEX.
  14. "Circadian gene Bmal1 regulates diurnal oscillations of Ly6C(hi) inflammatory monocytes."
    Nguyen K.D., Fentress S.J., Qiu Y., Yun K., Cox J.S., Chawla A.
    Science 341:1483-1488(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ARNTL/BMAL1.

Entry informationi

Entry nameiSUZ12_MOUSE
AccessioniPrimary (citable) accession number: Q80U70
Secondary accession number(s): B1AQE5, Q80Y10, Q99L07
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2003
Last sequence update: July 11, 2003
Last modified: June 8, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.