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Protein

Mitofusin-2

Gene

Mfn2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion (PubMed:12527753, PubMed:23921378, PubMed:23620051). Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events. Overexpression induces the formation of mitochondrial networks. Membrane clustering requires GTPase activity and may involve a major rearrangement of the coiled coil domains (By similarity). Plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Plays an important role in the regulation of vascular smooth muscle cell proliferation (By similarity). Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PRKN recruitment to dysfunctional mitochondria (PubMed:23620051). Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress (PubMed:23921556). Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions (PubMed:23921556).By similarity4 Publications

Catalytic activityi

GTP + H2O = GDP + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei305GTPBy similarity1
Binding sitei307GTPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi106 – 111GTPBy similarity6
Nucleotide bindingi258 – 261GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • blastocyst formation Source: MGI
  • camera-type eye morphogenesis Source: MGI
  • mitochondrial fusion Source: UniProtKB
  • mitochondrial membrane organization Source: UniProtKB
  • mitochondrion localization Source: UniProtKB
  • negative regulation of Ras protein signal transduction Source: MGI
  • negative regulation of smooth muscle cell proliferation Source: UniProtKB
  • parkin-mediated stimulation of mitophagy in response to mitochondrial depolarization Source: ParkinsonsUK-UCL
  • protein localization to pre-autophagosomal structure Source: MGI
  • protein targeting to mitochondrion Source: UniProtKB
  • response to unfolded protein Source: UniProtKB-KW

Keywordsi

Molecular functionDevelopmental protein, Hydrolase
Biological processApoptosis, Autophagy, Unfolded protein response
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-5205685. Pink/Parkin Mediated Mitophagy.
R-MMU-983231. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitofusin-2 (EC:3.6.5.-)
Alternative name(s):
Hypertension-related protein 1
Mitochondrial assembly regulatory factor
Short name:
HSG protein
Transmembrane GTPase MFN2
Gene namesi
Name:Mfn2
Synonyms:Kiaa0214, Marf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2442230. Mfn2.

Subcellular locationi

  • Mitochondrion outer membrane By similarity; Multi-pass membrane protein By similarity

  • Note: Colocalizes with BAX during apoptosis.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 604CytoplasmicSequence analysisAdd BLAST604
Transmembranei605 – 625Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini626Mitochondrial intermembraneSequence analysis1
Transmembranei627 – 647Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini648 – 757CytoplasmicSequence analysisAdd BLAST110

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • integral component of membrane Source: GO_Central
  • intrinsic component of mitochondrial outer membrane Source: UniProtKB
  • microtubule cytoskeleton Source: MGI
  • mitochondrion Source: MGI

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Disruption phenotypei

Mutant mice die early during embryonic development (PubMed:12527753). Heart-specific disruption causes cardiomyopathy in aging mice, characterized by dilated hearts that are insensitive to beta-adrenergic stimulation and impaired contractile performance (PubMed:23620051). Mutant cardiomyocytes display mitochondrial enlargement with respiratory impairment (PubMed:23620051).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001276761 – 757Mitofusin-2Add BLAST757

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei111Phosphothreonine; by PINK1By similarity1
Modified residuei442PhosphoserineCombined sources1
Modified residuei442Phosphoserine; by PINK1By similarity1

Post-translational modificationi

Phosphorylated by PINK1.1 Publication
Ubiquitinated by non-degradative ubiquitin by PRKN, promoting mitochondrial fusion; deubiquitination by USP30 inhibits mitochondrial fusion.2 Publications

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ80U63.
PaxDbiQ80U63.
PeptideAtlasiQ80U63.
PRIDEiQ80U63.

PTM databases

iPTMnetiQ80U63.
PhosphoSitePlusiQ80U63.

Expressioni

Tissue specificityi

Ubiquitous. Expression is markedly reduced in ApoE-knockout mouse atherosclerotic arteries.2 Publications

Gene expression databases

BgeeiENSMUSG00000029020.
CleanExiMM_MFN2.
ExpressionAtlasiQ80U63. baseline and differential.
GenevisibleiQ80U63. MM.

Interactioni

Subunit structurei

Forms homomultimers and heteromultimers with MFN1 (PubMed:12527753). Oligomerization is essential for mitochondrion fusion (Probable). Interacts with VAT1 (By similarity). Interacts with STOML2; may form heterooligomers (By similarity). Interacts (phosphorylated) with PRKN (PubMed:23620051). Interacts with EIF2AK3 (By similarity).By similarityCurated2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Eif2ak3Q9Z2B52EBI-8437663,EBI-1226344

GO - Molecular functioni

Protein-protein interaction databases

BioGridi228398. 4 interactors.
DIPiDIP-60970N.
IntActiQ80U63. 3 interactors.
MINTiMINT-8057620.
STRINGi10090.ENSMUSP00000030884.

Structurei

3D structure databases

ProteinModelPortaliQ80U63.
SMRiQ80U63.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini93 – 342Dynamin-type GAdd BLAST250

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni30 – 94Part of a helix bundle domain, formed by helices from N-terminal and C-terminal regionsBy similarityAdd BLAST65
Regioni359 – 385Part of a helix bundle domain, formed by helices from N-terminal and C-terminal regionsBy similarityAdd BLAST27
Regioni722 – 753Part of a helix bundle domain, formed by helices from N-terminal and C-terminal regionsBy similarityAdd BLAST32

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili406 – 434Sequence analysisAdd BLAST29
Coiled coili696 – 738Sequence analysisAdd BLAST43

Domaini

A helix bundle is formed by helices from the N-terminal and the C-terminal part of the protein. The GTPase domain cannot be expressed by itself, without the helix bundle. Rearrangement of the helix bundle and/or of the coiled coil domains may bring membranes from adjacent mitochondria into close contact, and thereby play a role in mitochondrial fusion.By similarity

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0448. Eukaryota.
COG0699. LUCA.
GeneTreeiENSGT00390000013727.
HOVERGENiHBG052465.
InParanoidiQ80U63.
KOiK06030.
OMAiEQRVYCL.
OrthoDBiEOG091G02ES.
PhylomeDBiQ80U63.
TreeFamiTF314289.

Family and domain databases

InterProiView protein in InterPro
IPR022812. Dynamin_SF.
IPR006884. Fzo/mitofusin_HR2.
IPR030381. G_DYNAMIN_dom.
IPR027089. Mitofusin-2.
IPR027417. P-loop_NTPase.
PANTHERiPTHR10465:SF9. PTHR10465:SF9. 1 hit.
PfamiView protein in Pfam
PF00350. Dynamin_N. 1 hit.
PF04799. Fzo_mitofusin. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS51718. G_DYNAMIN_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80U63-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLLFSRCNS IVTVKKDKRH MAEVNASPLK HFVTAKKKIN GIFEQLGAYI
60 70 80 90 100
QESASFLEDT HRNTELDPVT TEEQVLDVKG YLSKVRGISE VLARRHMKVA
110 120 130 140 150
FFGRTSNGKS TVINAMLWDK VLPSGIGHTT NCFLRVGGTD GHEAFLLTEG
160 170 180 190 200
SEEKKSVKTV NQLAHALHQD EQLHAGSMVS VMWPNSKCPL LKDDLVLMDS
210 220 230 240 250
PGIDVTTELD SWIDKFCLDA DVFVLVANSE STLMQTEKQF FHKVSERLSR
260 270 280 290 300
PNIFILNNRW DASASEPEYM EEVRRQHMER CTSFLVDELG VVDRAQAGDR
310 320 330 340 350
IFFVSAKEVL SARVQKAQGM PEGGGALAEG FQVRMFEFQN FERQFEECIS
360 370 380 390 400
QSAVKTKFEQ HTVRAKQIAE AVRLIMDSLH IAAQEQRVYC LEMREERQDR
410 420 430 440 450
LRFIDKQLEL LAQDYKLRIK QITEEVERQV STAMAEEIRR LSVLVDEYQM
460 470 480 490 500
DFHPSPVVLK VYKNELHRHI EEGLGRNLSD RCSTAIASSL QTMQQDMIDG
510 520 530 540 550
LKPLLPVSMR NQIDMLVPRQ CFSLSYDLNC DKLCADFQED IEFHFSLGWT
560 570 580 590 600
MLVNRFLGPK NSRRALLGYS DQVQRPLPLT PANPSMPPLP QSSLTQEELM
610 620 630 640 650
VSMVTGLASL TSRTSMGILV VGGVVWKAVG WRLIALSFGL YGLLYVYERL
660 670 680 690 700
TWTTKAKERA FKRQFVEYAS EKLQLIISYT GSNCSHQVQQ ELSGTFAHLC
710 720 730 740 750
QQVDITRDNL EQEIAAMNKK VEALDSLQSR AKLLRNKAGW LDSELNMFTH

QYLQPSR
Length:757
Mass (Da):86,188
Last modified:May 24, 2004 - v3
Checksum:i25B0EAE8301F4D1A
GO
Isoform 2 (identifier: Q80U63-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     573-605: VQRPLPLTPANPSMPPLPQSSLTQEELMVSMVT → ARSSFPWCIMGDHPDTRYGSQSTTAGVLRAEAI
     606-757: Missing.

Note: No experimental confirmation available.
Show »
Length:605
Mass (Da):68,938
Checksum:iEDCB31AC12D60912
GO

Sequence cautioni

The sequence BAC65502 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti123P → L in AAH46503 (PubMed:15489334).Curated1
Sequence conflicti167L → I in BAC33826 (PubMed:16141072).Curated1
Sequence conflicti332Q → K in AAK27416 (PubMed:12598526).Curated1
Sequence conflicti344Q → R in BAB39351 (Ref. 3) Curated1
Sequence conflicti344Q → R in AAK66559 (PubMed:15322553).Curated1
Sequence conflicti437E → G in BAB39351 (Ref. 3) Curated1
Sequence conflicti570S → N in AAK27416 (PubMed:12598526).Curated1
Sequence conflicti592S → G in AAK27416 (PubMed:12598526).Curated1
Sequence conflicti601V → G in AAK27416 (PubMed:12598526).Curated1
Sequence conflicti633 – 635LIA → IIP in AAK27416 (PubMed:12598526).Curated3
Sequence conflicti656A → T in AAK27416 (PubMed:12598526).Curated1
Sequence conflicti659R → K in AAK27416 (PubMed:12598526).Curated1
Sequence conflicti666V → A in AAK66559 (PubMed:15322553).Curated1
Sequence conflicti667E → D in BAB39351 (Ref. 3) Curated1
Sequence conflicti676I → T in BAB39351 (Ref. 3) Curated1
Sequence conflicti697A → G in AAK27416 (PubMed:12598526).Curated1
Sequence conflicti730R → K in AAK27416 (PubMed:12598526).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010365573 – 605VQRPL…VSMVT → ARSSFPWCIMGDHPDTRYGS QSTTAGVLRAEAI in isoform 2. 1 PublicationAdd BLAST33
Alternative sequenceiVSP_010366606 – 757Missing in isoform 2. 1 PublicationAdd BLAST152

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY123975 mRNA. Translation: AAM88577.1.
AY028170 mRNA. Translation: AAK27416.1.
AB048831 mRNA. Translation: BAB39351.1.
AF384100 mRNA. Translation: AAK66559.1.
AK042080 mRNA. Translation: BAE20620.1.
AK049583 mRNA. Translation: BAC33826.1.
AK052689 mRNA. Translation: BAC35096.1.
AK122220 Transcribed RNA. Translation: BAC65502.2. Different initiation.
AL607066 Genomic DNA. Translation: CAM23513.1.
AL607066 Genomic DNA. Translation: CAP19097.1.
BC046503 mRNA. Translation: AAH46503.1.
CCDSiCCDS38965.1. [Q80U63-1]
RefSeqiNP_001272849.1. NM_001285920.1. [Q80U63-1]
NP_001272850.1. NM_001285921.1. [Q80U63-1]
NP_001272851.1. NM_001285922.1. [Q80U63-1]
NP_001272852.1. NM_001285923.1. [Q80U63-1]
NP_573464.2. NM_133201.3. [Q80U63-1]
XP_006538672.1. XM_006538609.3. [Q80U63-1]
XP_006538673.1. XM_006538610.2. [Q80U63-1]
XP_006538674.1. XM_006538611.3. [Q80U63-1]
UniGeneiMm.154312.
Mm.437499.

Genome annotation databases

EnsembliENSMUST00000030884; ENSMUSP00000030884; ENSMUSG00000029020. [Q80U63-1]
ENSMUST00000105714; ENSMUSP00000101339; ENSMUSG00000029020. [Q80U63-2]
ENSMUST00000105715; ENSMUSP00000101340; ENSMUSG00000029020. [Q80U63-1]
ENSMUST00000105716; ENSMUSP00000101341; ENSMUSG00000029020. [Q80U63-1]
GeneIDi170731.
KEGGimmu:170731.
UCSCiuc008vtg.2. mouse. [Q80U63-1]
uc008vtj.2. mouse. [Q80U63-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY123975 mRNA. Translation: AAM88577.1.
AY028170 mRNA. Translation: AAK27416.1.
AB048831 mRNA. Translation: BAB39351.1.
AF384100 mRNA. Translation: AAK66559.1.
AK042080 mRNA. Translation: BAE20620.1.
AK049583 mRNA. Translation: BAC33826.1.
AK052689 mRNA. Translation: BAC35096.1.
AK122220 Transcribed RNA. Translation: BAC65502.2. Different initiation.
AL607066 Genomic DNA. Translation: CAM23513.1.
AL607066 Genomic DNA. Translation: CAP19097.1.
BC046503 mRNA. Translation: AAH46503.1.
CCDSiCCDS38965.1. [Q80U63-1]
RefSeqiNP_001272849.1. NM_001285920.1. [Q80U63-1]
NP_001272850.1. NM_001285921.1. [Q80U63-1]
NP_001272851.1. NM_001285922.1. [Q80U63-1]
NP_001272852.1. NM_001285923.1. [Q80U63-1]
NP_573464.2. NM_133201.3. [Q80U63-1]
XP_006538672.1. XM_006538609.3. [Q80U63-1]
XP_006538673.1. XM_006538610.2. [Q80U63-1]
XP_006538674.1. XM_006538611.3. [Q80U63-1]
UniGeneiMm.154312.
Mm.437499.

3D structure databases

ProteinModelPortaliQ80U63.
SMRiQ80U63.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228398. 4 interactors.
DIPiDIP-60970N.
IntActiQ80U63. 3 interactors.
MINTiMINT-8057620.
STRINGi10090.ENSMUSP00000030884.

PTM databases

iPTMnetiQ80U63.
PhosphoSitePlusiQ80U63.

Proteomic databases

EPDiQ80U63.
PaxDbiQ80U63.
PeptideAtlasiQ80U63.
PRIDEiQ80U63.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030884; ENSMUSP00000030884; ENSMUSG00000029020. [Q80U63-1]
ENSMUST00000105714; ENSMUSP00000101339; ENSMUSG00000029020. [Q80U63-2]
ENSMUST00000105715; ENSMUSP00000101340; ENSMUSG00000029020. [Q80U63-1]
ENSMUST00000105716; ENSMUSP00000101341; ENSMUSG00000029020. [Q80U63-1]
GeneIDi170731.
KEGGimmu:170731.
UCSCiuc008vtg.2. mouse. [Q80U63-1]
uc008vtj.2. mouse. [Q80U63-2]

Organism-specific databases

CTDi9927.
MGIiMGI:2442230. Mfn2.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0448. Eukaryota.
COG0699. LUCA.
GeneTreeiENSGT00390000013727.
HOVERGENiHBG052465.
InParanoidiQ80U63.
KOiK06030.
OMAiEQRVYCL.
OrthoDBiEOG091G02ES.
PhylomeDBiQ80U63.
TreeFamiTF314289.

Enzyme and pathway databases

ReactomeiR-MMU-5205685. Pink/Parkin Mediated Mitophagy.
R-MMU-983231. Factors involved in megakaryocyte development and platelet production.

Miscellaneous databases

PROiPR:Q80U63.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029020.
CleanExiMM_MFN2.
ExpressionAtlasiQ80U63. baseline and differential.
GenevisibleiQ80U63. MM.

Family and domain databases

InterProiView protein in InterPro
IPR022812. Dynamin_SF.
IPR006884. Fzo/mitofusin_HR2.
IPR030381. G_DYNAMIN_dom.
IPR027089. Mitofusin-2.
IPR027417. P-loop_NTPase.
PANTHERiPTHR10465:SF9. PTHR10465:SF9. 1 hit.
PfamiView protein in Pfam
PF00350. Dynamin_N. 1 hit.
PF04799. Fzo_mitofusin. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS51718. G_DYNAMIN_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMFN2_MOUSE
AccessioniPrimary (citable) accession number: Q80U63
Secondary accession number(s): A2A7Y7
, A8Y5E4, Q3V3B8, Q80WP4, Q80XK3, Q8BHF0, Q8BKV5, Q8R535, Q923X2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 24, 2004
Last modified: June 7, 2017
This is version 127 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.