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Protein

Run domain Beclin-1-interacting and cysteine-rich domain-containing protein

Gene

Rubcn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits PIK3C3 activity; under basal conditions negatively regulates PI3K complex II (PI3KC3-C2) function in autophagy. Negatively regulates endosome maturation and degradative endocytic trafficking and impairs autophagosome maturation process (PubMed:19270693). Can sequester UVRAG from association with a class C Vps complex (possibly the HOPS complex) and negatively regulates Rab7 activation (By similarity).By similarity1 Publication
Involved in regulation of pathogen-specific host defense of activated macrophages. Following bacterial infection promotes NADH oxidase activity by association with CYBA thereby affecting TLR2 signaling and probably other TLR-NOX pathways. Stabilizes the CYBA:CYBB NADPH oxidase heterodimer, increases its association with TLR2 and its phagosome trafficking to induce antimicrobial burst of ROS and production of inflammatory cytokines. Following fungal or viral infection (implicating CLEC7A (dectin-1)-mediated myeloid cell activation or DDX58/RIG-I-dependent sensing of RNA viruses) negatively regulates pro-inflammatory cytokine production by association with CARD9 and sequestering it from signaling complexes (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Autophagy, Endocytosis, Immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Run domain Beclin-1-interacting and cysteine-rich domain-containing proteinBy similarity
Short name:
Rubicon
Gene namesi
Name:RubcnBy similarity
Synonyms:Kiaa0226
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1915160. Rubcn.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endosome, Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000507371 – 956Run domain Beclin-1-interacting and cysteine-rich domain-containing proteinAdd BLAST956

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei198PhosphoserineBy similarity1
Modified residuei250PhosphoserineBy similarity1
Modified residuei268PhosphoserineBy similarity1
Modified residuei390PhosphoserineCombined sources1
Modified residuei412PhosphoserineCombined sources1
Modified residuei513PhosphoserineBy similarity1
Modified residuei547PhosphoserineBy similarity1
Modified residuei655PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ80U62.
PRIDEiQ80U62.

PTM databases

iPTMnetiQ80U62.
PhosphoSitePlusiQ80U62.

Expressioni

Gene expression databases

BgeeiENSMUSG00000035629.
CleanExiMM_1700021K19RIK.
ExpressionAtlasiQ80U62. baseline and differential.
GenevisibleiQ80U62. MM.

Interactioni

Subunit structurei

Associates with PI3K (PI3KC3/PI3K-III/class III phosphatidylinositol 3-kinase) complex II (PI3KC3-C2) in which the core composed of the catalytic subunit PIK3C3, the regulatory subunit PIK3R4 and BECN1 is associated with UVRAG; in the complex interacts directly with PI3KC3 and UVRAG (PubMed:19270693). Interacts with Rab7 (RAB7A or RAB7B) (GTP-bound form); Rab7 and UVRAG compete for RUBCN binding; can interact simultaneously with Rab7 and the PI3K complex. Interacts with CYBA and CYBB; indicative for the association with the CYBA:CYBB NADPH oxidase heterodimer. Interacts with NOX4 and probably associates with the CYBA:NOX4 complex. Interacts with YWHAB and CARD9 in a competetive and stimulation-dependent manner; RUBCN exchanges interaction from YWHAB to CARD9 upon stimulation with beta-1,3-glucan (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
EGFRP005332EBI-3506572,EBI-297353From a different organism.

Protein-protein interaction databases

IntActiQ80U62. 5 interactors.
STRINGi10090.ENSMUSP00000087114.

Structurei

3D structure databases

ProteinModelPortaliQ80U62.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini49 – 190RUNPROSITE-ProRule annotationAdd BLAST142

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni50 – 181Interaction with PIK3C3By similarityAdd BLAST132
Regioni205 – 437Interaction with YWHABBy similarityAdd BLAST233
Regioni302 – 585Interaction with UVRAGBy similarityAdd BLAST284
Regioni490 – 542Interaction with BECN1By similarityAdd BLAST53
Regioni552 – 609Interaction with CYBABy similarityAdd BLAST58
Regioni656 – 744Interaction with CARD9By similarityAdd BLAST89
Regioni705 – 956Interaction with Rab7By similarityAdd BLAST252

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi226 – 392Ser-richAdd BLAST167
Compositional biasi552 – 555Poly-Ser4
Compositional biasi865 – 916Cys-richAdd BLAST52

Sequence similaritiesi

Contains 1 RUN domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1829. Eukaryota.
KOG4381. Eukaryota.
ENOG410XQ7T. LUCA.
GeneTreeiENSGT00550000074570.
HOVERGENiHBG062207.
InParanoidiQ80U62.
KOiK19330.
OMAiLYHGLIH.
OrthoDBiEOG091G05ZY.
PhylomeDBiQ80U62.
TreeFamiTF317067.

Family and domain databases

InterProiIPR004012. Run_dom.
IPR025258. Zf-RING_9.
[Graphical view]
PfamiPF02759. RUN. 1 hit.
PF13901. zf-RING_9. 1 hit.
[Graphical view]
SMARTiSM01175. DUF4206. 1 hit.
SM00593. RUN. 1 hit.
[Graphical view]
PROSITEiPS50826. RUN. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80U62-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRPEGAGMDL GGGDGERLLE KSRREHWQLL GNLKTTVEGL VSANCPNVWS
60 70 80 90 100
KYGGLERLCR DMQNILYHGL IHDQVCCRQA DYWQFVKDIR WLSPHSALHV
110 120 130 140 150
EKFISLHESD QSDTDSVSER AVAELWLQHS LQCHCLSAQL RPLLGDRQYI
160 170 180 190 200
RKFYTETAFL LSDAHVTAML QCLEAVEQNN PRLLAQIDAS MFARKQESPL
210 220 230 240 250
LVTKSQSLTA LPGSTYTPPA SYAQHSYFGS SSSLQSMPQS SHSSERRSTS
260 270 280 290 300
FSLSGPSWQP QEDRECLSPA ETQTTPAPLP SDSTLAQDSP LTAQEMSDST
310 320 330 340 350
LTSPLEASWV SSQNDSPSDV SEGPEYLAIG NPAPHGRTAS CESHSSNGES
360 370 380 390 400
SSSHLFSSSS SQKLESAASS LGDQEEGRQS QAGSVLRRSS FSEGQTAPVA
410 420 430 440 450
SGTKKSHIRS HSDTNIASRG AAGGPRNITI IVEDPIAEGG QYLCSGEGMF
460 470 480 490 500
RRPSEGQSLI SYLSEQDFGS CADLEKENAH FSISESLIAA IELMKCNMMS
510 520 530 540 550
QCLEEEEVEE EDSDREIQEL KQKIRLRRQQ IRTKNLLPAY RETENGSFRV
560 570 580 590 600
TSSSSQFSSR DSTQLSESGS AEDADDLEIQ DADIRRSAVS NGKSSFSQNL
610 620 630 640 650
SHCFLHSTSA EAVAMGLLKQ FEGMQLPAAS ELEWLVPEHD APQKLLPIPD
660 670 680 690 700
SLPISPDDGQ HADIYKLRIR VRGNLEWAPP RPQIIFNVHP APTRKIAVAK
710 720 730 740 750
QNYRCAGCGI RTDPDYIKRL RYCEYLGKYF CQCCHENAQM VVPSRILRKW
760 770 780 790 800
DFSKYYVSNF SKDLLLKIWN DPLFNVQDIN SALYRKVKLL NQVRLLRVQL
810 820 830 840 850
YHMKNMFKTC RLAKELLDSF DVVPGHLTED LHLYSLSDLT ATKKGELGPR
860 870 880 890 900
LAELTRAGAA HVERCMLCQA KGFICEFCQN EEDVIFPFEL HKCRTCEECK
910 920 930 940 950
ACYHKTCFKS GRCPRCERLQ ARRELLAKQS LESYLSDYEE EPTEALALEA

TVLETT
Length:956
Mass (Da):106,860
Last modified:January 24, 2006 - v2
Checksum:iE5D8CD691C0D5184
GO
Isoform 2 (identifier: Q80U62-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     423-437: Missing.

Show »
Length:941
Mass (Da):105,313
Checksum:iD3B091AE60EF0A3B
GO
Isoform 3 (identifier: Q80U62-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     347-360: Missing.
     423-437: Missing.

Show »
Length:927
Mass (Da):103,919
Checksum:i384DB3B3F5448D8D
GO
Isoform 4 (identifier: Q80U62-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: Missing.
     347-360: Missing.
     423-437: Missing.

Note: No experimental confirmation available.
Show »
Length:866
Mass (Da):97,179
Checksum:iA2E4D53DF257399A
GO

Sequence cautioni

The sequence AAH57307 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAC65503 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5G → S in BAE41507 (PubMed:16141072).Curated1
Sequence conflicti5G → S in BAE41645 (PubMed:16141072).Curated1
Sequence conflicti88D → G in AAH67390 (PubMed:19468303).Curated1
Sequence conflicti217T → N in BAE41507 (PubMed:16141072).Curated1
Sequence conflicti217T → N in BAE41645 (PubMed:16141072).Curated1
Sequence conflicti217T → N in BAE41708 (PubMed:16141072).Curated1
Sequence conflicti248S → P in BAE41645 (PubMed:16141072).Curated1
Sequence conflicti298D → G in BAE41708 (PubMed:16141072).Curated1
Sequence conflicti304P → S in AAH67390 (PubMed:19468303).Curated1
Sequence conflicti379Q → L in AAH67390 (PubMed:19468303).Curated1
Sequence conflicti470S → G in BAE42541 (PubMed:16141072).Curated1
Sequence conflicti628A → T in AAH67390 (PubMed:19468303).Curated1
Sequence conflicti661H → Q in BAE42541 (PubMed:16141072).Curated1
Sequence conflicti865C → R in AAH67390 (PubMed:19468303).Curated1
Sequence conflicti901A → T in BAE41708 (PubMed:16141072).Curated1
Sequence conflicti951T → P in BAC31257 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0394741 – 61Missing in isoform 4. 1 PublicationAdd BLAST61
Alternative sequenceiVSP_017110347 – 360Missing in isoform 3 and isoform 4. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_017111423 – 437Missing in isoform 2, isoform 3 and isoform 4. 2 PublicationsAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122221 mRNA. Translation: BAC65503.1. Different initiation.
AK030368 mRNA. Translation: BAC26925.1.
AK042428 mRNA. Translation: BAC31257.1.
AK170002 mRNA. Translation: BAE41507.1.
AK170223 mRNA. Translation: BAE41645.1.
AK170312 mRNA. Translation: BAE41708.1.
AK171583 mRNA. Translation: BAE42541.1.
AC139244 Genomic DNA. No translation available.
BC057307 mRNA. Translation: AAH57307.1. Different initiation.
BC060601 mRNA. Translation: AAH60601.1.
BC067390 mRNA. Translation: AAH67390.1.
CCDSiCCDS28124.1. [Q80U62-2]
CCDS49832.1. [Q80U62-1]
RefSeqiNP_001186967.1. NM_001200038.1. [Q80U62-1]
NP_766203.1. NM_172615.4. [Q80U62-2]
UniGeneiMm.327319.

Genome annotation databases

EnsembliENSMUST00000040986; ENSMUSP00000048811; ENSMUSG00000035629. [Q80U62-2]
ENSMUST00000089684; ENSMUSP00000087114; ENSMUSG00000035629. [Q80U62-1]
ENSMUST00000115105; ENSMUSP00000110757; ENSMUSG00000035629. [Q80U62-3]
GeneIDi100502698.
KEGGimmu:100502698.
UCSCiuc007yzk.2. mouse. [Q80U62-1]
uc007yzl.2. mouse. [Q80U62-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122221 mRNA. Translation: BAC65503.1. Different initiation.
AK030368 mRNA. Translation: BAC26925.1.
AK042428 mRNA. Translation: BAC31257.1.
AK170002 mRNA. Translation: BAE41507.1.
AK170223 mRNA. Translation: BAE41645.1.
AK170312 mRNA. Translation: BAE41708.1.
AK171583 mRNA. Translation: BAE42541.1.
AC139244 Genomic DNA. No translation available.
BC057307 mRNA. Translation: AAH57307.1. Different initiation.
BC060601 mRNA. Translation: AAH60601.1.
BC067390 mRNA. Translation: AAH67390.1.
CCDSiCCDS28124.1. [Q80U62-2]
CCDS49832.1. [Q80U62-1]
RefSeqiNP_001186967.1. NM_001200038.1. [Q80U62-1]
NP_766203.1. NM_172615.4. [Q80U62-2]
UniGeneiMm.327319.

3D structure databases

ProteinModelPortaliQ80U62.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ80U62. 5 interactors.
STRINGi10090.ENSMUSP00000087114.

PTM databases

iPTMnetiQ80U62.
PhosphoSitePlusiQ80U62.

Proteomic databases

PaxDbiQ80U62.
PRIDEiQ80U62.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040986; ENSMUSP00000048811; ENSMUSG00000035629. [Q80U62-2]
ENSMUST00000089684; ENSMUSP00000087114; ENSMUSG00000035629. [Q80U62-1]
ENSMUST00000115105; ENSMUSP00000110757; ENSMUSG00000035629. [Q80U62-3]
GeneIDi100502698.
KEGGimmu:100502698.
UCSCiuc007yzk.2. mouse. [Q80U62-1]
uc007yzl.2. mouse. [Q80U62-2]

Organism-specific databases

CTDi9711.
MGIiMGI:1915160. Rubcn.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1829. Eukaryota.
KOG4381. Eukaryota.
ENOG410XQ7T. LUCA.
GeneTreeiENSGT00550000074570.
HOVERGENiHBG062207.
InParanoidiQ80U62.
KOiK19330.
OMAiLYHGLIH.
OrthoDBiEOG091G05ZY.
PhylomeDBiQ80U62.
TreeFamiTF317067.

Miscellaneous databases

PROiQ80U62.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035629.
CleanExiMM_1700021K19RIK.
ExpressionAtlasiQ80U62. baseline and differential.
GenevisibleiQ80U62. MM.

Family and domain databases

InterProiIPR004012. Run_dom.
IPR025258. Zf-RING_9.
[Graphical view]
PfamiPF02759. RUN. 1 hit.
PF13901. zf-RING_9. 1 hit.
[Graphical view]
SMARTiSM01175. DUF4206. 1 hit.
SM00593. RUN. 1 hit.
[Graphical view]
PROSITEiPS50826. RUN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRUBIC_MOUSE
AccessioniPrimary (citable) accession number: Q80U62
Secondary accession number(s): Q3TAX3
, Q3TD96, Q3TDF7, Q3TDU2, Q6NWW8, Q6P9T7, Q6PG18, Q8BMP7, Q8BY22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: January 24, 2006
Last modified: November 2, 2016
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.