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Protein

Pumilio homolog 2

Gene

Pum2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs. Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation. Plays a role in cytoplasmic sensing of viral infection. Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD (non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm. May regulate DCUN1D3 mRNA levels. May support proliferation and self-renewal of stem cells.By similarity

GO - Molecular functioni

  • mRNA 3'-UTR binding Source: MGI
  • poly(A) RNA binding Source: MGI
  • RNA binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Translation regulation

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pumilio homolog 2
Gene namesi
Name:Pum2
Synonyms:Kiaa0235
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1931751. Pum2.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Cytoplasmic granule 1 Publication
  • Cytoplasmperinuclear region By similarity

  • Note: The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS1 and SNAPIN in the perinuclear region of germ cells (By similarity). Colocalizes with NANOS3 in the stress granules (PubMed:19861488).By similarity1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000759201 – 1066Pumilio homolog 2Add BLAST1066

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei67PhosphoserineBy similarity1
Modified residuei82PhosphoserineCombined sources1
Modified residuei136PhosphoserineCombined sources1
Modified residuei177PhosphoserineBy similarity1
Modified residuei181PhosphoserineBy similarity1
Modified residuei183PhosphothreonineBy similarity1
Modified residuei395PhosphothreonineBy similarity1
Modified residuei587PhosphoserineCombined sources1
Modified residuei700PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ80U58.
PeptideAtlasiQ80U58.
PRIDEiQ80U58.

PTM databases

iPTMnetiQ80U58.
PhosphoSitePlusiQ80U58.

Expressioni

Tissue specificityi

Widely expressed. Expressed in embryonic stem cells, heart, kidney, lung, skin, intestine, spleen and thymus. Expressed at intermediate level in brain and liver. Weakly or not expressed in muscles and stomach. Expressed at various stages of myeloid and lymphoid cell development. In the testis expressed in the spermatogoni, spermatocytes, spermatids and Sertoli cells.3 Publications

Gene expression databases

BgeeiENSMUSG00000020594.
CleanExiMM_PUM2.
ExpressionAtlasiQ80U58. baseline and differential.
GenevisibleiQ80U58. MM.

Interactioni

Subunit structurei

Homodimer; homodimerizes in vitro. Interacts with DAZ, DAZL and NANOS1 via its pumilio repeats. Interacts with NANOS3 (By similarity). Interacts with SNAPIN. Recruits the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Interacts with DDX20. In case of viral infection, interacts with DHX58 (By similarity).By similarity

Protein-protein interaction databases

BioGridi219850. 3 interactors.
IntActiQ80U58. 3 interactors.
STRINGi10090.ENSMUSP00000128292.

Structurei

Secondary structure

11066
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi709 – 715Combined sources7
Helixi724 – 727Combined sources4
Turni728 – 730Combined sources3
Helixi731 – 735Combined sources5
Helixi738 – 748Combined sources11
Helixi753 – 763Combined sources11
Helixi764 – 766Combined sources3
Helixi767 – 771Combined sources5
Helixi776 – 786Combined sources11
Helixi789 – 799Combined sources11
Helixi803 – 807Combined sources5
Helixi812 – 822Combined sources11
Helixi825 – 828Combined sources4
Helixi831 – 834Combined sources4
Helixi835 – 837Combined sources3
Helixi841 – 846Combined sources6
Helixi850 – 860Combined sources11
Helixi863 – 865Combined sources3
Helixi867 – 872Combined sources6
Turni873 – 876Combined sources4
Helixi877 – 881Combined sources5
Helixi886 – 896Combined sources11
Helixi899 – 910Combined sources12
Helixi913 – 917Combined sources5
Helixi922 – 932Combined sources11
Helixi935 – 943Combined sources9
Turni944 – 947Combined sources4
Helixi949 – 953Combined sources5
Helixi958 – 968Combined sources11
Helixi971 – 982Combined sources12
Helixi991 – 997Combined sources7
Helixi1001 – 1011Combined sources11
Helixi1014 – 1024Combined sources11
Helixi1025 – 1027Combined sources3
Helixi1028 – 1031Combined sources4
Turni1035 – 1037Combined sources3
Helixi1038 – 1047Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GVOX-ray1.60A706-1056[»]
3GVTX-ray2.80A/B706-1056[»]
ProteinModelPortaliQ80U58.
SMRiQ80U58.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ80U58.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini706 – 1048PUM-HDPROSITE-ProRule annotationAdd BLAST343
Repeati726 – 761Pumilio 1Add BLAST36
Repeati762 – 797Pumilio 2Add BLAST36
Repeati798 – 835Pumilio 3Add BLAST38
Repeati836 – 871Pumilio 4Add BLAST36
Repeati872 – 907Pumilio 5Add BLAST36
Repeati908 – 943Pumilio 6Add BLAST36
Repeati944 – 979Pumilio 7Add BLAST36
Repeati980 – 1022Pumilio 8Add BLAST43

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 260Interaction with SNAPINBy similarityAdd BLAST260
Regioni741 – 745Adenine-nucleotide binding in RNA targetBy similarity5
Regioni777 – 781Uracil-nucleotide binding in RNA targetBy similarity5
Regioni813 – 817Adenine-nucleotide binding in RNA targetBy similarity5
Regioni851 – 855Non-specific-nucleotide binding in RNA targetBy similarity5
Regioni887 – 891Adenine-nucleotide binding in RNA targetBy similarity5
Regioni923 – 927Uracil-nucleotide binding in RNA targetBy similarity5
Regioni959 – 963Guanine-nucleotide binding in RNA targetBy similarity5
Regioni1002 – 1006Uracil-nucleotide binding in RNA targetBy similarity5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi277 – 486Ala-richAdd BLAST210
Compositional biasi358 – 404Gln-richAdd BLAST47
Compositional biasi520 – 687Ser-richAdd BLAST168

Domaini

The pumilio repeats mediate the association with RNA by packing together to form a right-handed superhelix that approximates a half donut. RNA-binding occurs on the concave side of the surface (PubMed:19540345). PUM2 is composed of 8 pumilio repeats of 36 residues; each repeat binds a single nucleotide in its RNA target. Residues at positions 12 and 16 of the pumilio repeat bind each RNA base via hydrogen bonding or van der Waals contacts with the Watson-Crick edge, while the amino acid at position 13 makes a stacking interaction. The recognition of RNA by pumilio repeats is base specific: cysteine and glutamine at position 12 and 16, respectively, bind adenine; asparagine and glutamine bind uracil; and serine and glutamate bind guanine (By similarity).By similarity1 Publication

Sequence similaritiesi

Contains 1 PUM-HD domain.PROSITE-ProRule annotation
Contains 8 pumilio repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1488. Eukaryota.
COG5099. LUCA.
GeneTreeiENSGT00390000017241.
HOGENOMiHOG000238461.
HOVERGENiHBG049462.
InParanoidiQ80U58.
KOiK17943.
OMAiLFDYSSQ.
OrthoDBiEOG091G04QO.
PhylomeDBiQ80U58.
TreeFamiTF318160.

Family and domain databases

CDDicd07920. Pumilio. 1 hit.
Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR033133. PUM-HD.
IPR033712. Pumilio_RNA-bd.
IPR001313. Pumilio_RNA-bd_rpt.
[Graphical view]
PfamiPF00806. PUF. 8 hits.
[Graphical view]
SMARTiSM00025. Pumilio. 8 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50302. PUM. 8 hits.
PS50303. PUM_HD. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80U58-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNHDFQALAL ESRGMGELLP TKKFWEPDDS TKDGQKGIFL GDDEWRETAW
60 70 80 90 100
GTSHHSMSQP IMVQRRSGQS FHGNSEVNAI LSPRSESGGL GVSMVEYVLS
110 120 130 140 150
SSPADKLDSR FRKGTFGTRD AETDGPEKGD QKGKASPFEE DQNRDLKQDD
160 170 180 190 200
EDSKINGRGL PNGMDADCKD FNRTPGSRQA SPTEVVERLG PSTNPPEGLG
210 220 230 240 250
PLPNPTANKP LVEEFSNPET QNLDAMDQVG LDSLQFDYPG NQVPMDSSGA
260 270 280 290 300
TVGLFDYNSQ QQLFQRTSAL TVQQLTAAQQ QQYALAAAQQ PHIAGVFSAG
310 320 330 340 350
LAPAAFVPNP YIISAAPPGT DPYTAAGLAA AATLAGPAVV PPQYYGVPWG
360 370 380 390 400
VYPANLFQQQ AAAAASNTAN QQAASQAQPG QQQVLRPGAG QRPITPSQGQ
410 420 430 440 450
QGQQAESLAA AANPTLAFGQ SLAAGMPGYQ VLAPTAYYDQ TGALVVGPGA
460 470 480 490 500
RTGLGAPVRL MAPTPVLISS TAAQAAAAAA AAGGTANSLT GSTNGLFRPI
510 520 530 540 550
GTQPPQQQQQ QQQPSTNLQS NSFYGSSSLT NSSQSSSLFS HGPGQPGSAS
560 570 580 590 600
LGFGSGSSLG AAIGSALSGF GSSVGSSASS SATRRESLST SSDLYKRSSS
610 620 630 640 650
SLAPIGQPFY NSLGFSSSPS PIGMPLPSQT PGHSLTPPPS LSSHGSSSSL
660 670 680 690 700
HLGGLTNGSG RYISAAPGAE AKYRSASSTS SLFSSSSQLF PPSRLRYNRS
710 720 730 740 750
DIMPSGRSRL LEDFRNNRFP NLQLRDLIGH IVEFSQDQHG SRFIQQKLER
760 770 780 790 800
ATPAERQIVF NEILQAAYQL MTDVFGNYVI QKFFEFGSLD QKLALATRIR
810 820 830 840 850
GHVLPLALQM YGCRVIQKAL ESISSDQQVI SEMVKELDGH VLKCVKDQNG
860 870 880 890 900
NHVVQKCIEC VQPQSLQFII DAFKGQVFVL STHPYGCRVI QRILEHCTAE
910 920 930 940 950
QTLPILEELH QHTEQLVQDQ YGNYVIQHVL EHGRPEDKSK IVSEIRGKVL
960 970 980 990 1000
ALSQHKFASN VVEKCVTHAS RAERALLIDE VCCQNDGPHS ALYTMMKDQY
1010 1020 1030 1040 1050
ANYVVQKMID MAEPAQRKII MHKIRPHITT LRKYTYGKHI LAKLEKYYLK
1060
NSPDLGPIGG PPNGML
Length:1,066
Mass (Da):114,314
Last modified:February 2, 2004 - v2
Checksum:i027AC00FA91B055E
GO
Isoform 2 (identifier: Q80U58-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     574-652: Missing.
     829-830: Missing.

Show »
Length:985
Mass (Da):106,101
Checksum:i4F4CBF32596ACC13
GO
Isoform 3 (identifier: Q80U58-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-56: Missing.
     574-652: Missing.
     829-830: Missing.

Note: No experimental confirmation available.
Show »
Length:929
Mass (Da):99,714
Checksum:iE179D3A4364053B4
GO

Sequence cautioni

The sequence BAC65507 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti461M → I in BAC65507 (PubMed:12693553).Curated1
Sequence conflicti547G → A in AAG31805 (PubMed:12667987).Curated1
Sequence conflicti553F → C in AAG31805 (PubMed:12667987).Curated1
Sequence conflicti559 – 560LG → VR in AAG31805 (PubMed:12667987).Curated2
Sequence conflicti570F → C in AAG31805 (PubMed:12667987).Curated1
Sequence conflicti592S → A in AAG31805 (PubMed:12667987).Curated1
Sequence conflicti628S → G in AAG31805 (PubMed:12667987).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0093211 – 56Missing in isoform 3. 1 PublicationAdd BLAST56
Alternative sequenceiVSP_009322574 – 652Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST79
Alternative sequenceiVSP_009323829 – 830Missing in isoform 2 and isoform 3. 2 Publications2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY027917 mRNA. Translation: AAK21966.1.
AF315590 mRNA. Translation: AAG31805.1.
AK122225 mRNA. Translation: BAC65507.1. Different initiation.
BC013765 mRNA. Translation: AAH13765.1.
BC041773 mRNA. Translation: AAH41773.1.
CCDSiCCDS25802.1. [Q80U58-1]
CCDS49026.1. [Q80U58-2]
RefSeqiNP_001153691.1. NM_001160219.1. [Q80U58-1]
NP_001153694.1. NM_001160222.1. [Q80U58-2]
NP_109648.2. NM_030723.2. [Q80U58-1]
XP_006515340.1. XM_006515277.2. [Q80U58-1]
XP_006515341.1. XM_006515278.3. [Q80U58-1]
XP_006515342.1. XM_006515279.2. [Q80U58-1]
XP_006515348.1. XM_006515285.3. [Q80U58-2]
UniGeneiMm.341243.

Genome annotation databases

EnsembliENSMUST00000163569; ENSMUSP00000131074; ENSMUSG00000020594. [Q80U58-1]
ENSMUST00000168361; ENSMUSP00000128292; ENSMUSG00000020594. [Q80U58-1]
ENSMUST00000169089; ENSMUSP00000132122; ENSMUSG00000020594. [Q80U58-2]
ENSMUST00000178015; ENSMUSP00000137020; ENSMUSG00000020594. [Q80U58-2]
GeneIDi80913.
KEGGimmu:80913.
UCSCiuc007mzw.2. mouse. [Q80U58-3]
uc007mzy.2. mouse. [Q80U58-1]
uc007naa.2. mouse. [Q80U58-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY027917 mRNA. Translation: AAK21966.1.
AF315590 mRNA. Translation: AAG31805.1.
AK122225 mRNA. Translation: BAC65507.1. Different initiation.
BC013765 mRNA. Translation: AAH13765.1.
BC041773 mRNA. Translation: AAH41773.1.
CCDSiCCDS25802.1. [Q80U58-1]
CCDS49026.1. [Q80U58-2]
RefSeqiNP_001153691.1. NM_001160219.1. [Q80U58-1]
NP_001153694.1. NM_001160222.1. [Q80U58-2]
NP_109648.2. NM_030723.2. [Q80U58-1]
XP_006515340.1. XM_006515277.2. [Q80U58-1]
XP_006515341.1. XM_006515278.3. [Q80U58-1]
XP_006515342.1. XM_006515279.2. [Q80U58-1]
XP_006515348.1. XM_006515285.3. [Q80U58-2]
UniGeneiMm.341243.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GVOX-ray1.60A706-1056[»]
3GVTX-ray2.80A/B706-1056[»]
ProteinModelPortaliQ80U58.
SMRiQ80U58.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219850. 3 interactors.
IntActiQ80U58. 3 interactors.
STRINGi10090.ENSMUSP00000128292.

PTM databases

iPTMnetiQ80U58.
PhosphoSitePlusiQ80U58.

Proteomic databases

PaxDbiQ80U58.
PeptideAtlasiQ80U58.
PRIDEiQ80U58.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000163569; ENSMUSP00000131074; ENSMUSG00000020594. [Q80U58-1]
ENSMUST00000168361; ENSMUSP00000128292; ENSMUSG00000020594. [Q80U58-1]
ENSMUST00000169089; ENSMUSP00000132122; ENSMUSG00000020594. [Q80U58-2]
ENSMUST00000178015; ENSMUSP00000137020; ENSMUSG00000020594. [Q80U58-2]
GeneIDi80913.
KEGGimmu:80913.
UCSCiuc007mzw.2. mouse. [Q80U58-3]
uc007mzy.2. mouse. [Q80U58-1]
uc007naa.2. mouse. [Q80U58-2]

Organism-specific databases

CTDi23369.
MGIiMGI:1931751. Pum2.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1488. Eukaryota.
COG5099. LUCA.
GeneTreeiENSGT00390000017241.
HOGENOMiHOG000238461.
HOVERGENiHBG049462.
InParanoidiQ80U58.
KOiK17943.
OMAiLFDYSSQ.
OrthoDBiEOG091G04QO.
PhylomeDBiQ80U58.
TreeFamiTF318160.

Miscellaneous databases

ChiTaRSiPum2. mouse.
EvolutionaryTraceiQ80U58.
PROiQ80U58.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020594.
CleanExiMM_PUM2.
ExpressionAtlasiQ80U58. baseline and differential.
GenevisibleiQ80U58. MM.

Family and domain databases

CDDicd07920. Pumilio. 1 hit.
Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR033133. PUM-HD.
IPR033712. Pumilio_RNA-bd.
IPR001313. Pumilio_RNA-bd_rpt.
[Graphical view]
PfamiPF00806. PUF. 8 hits.
[Graphical view]
SMARTiSM00025. Pumilio. 8 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50302. PUM. 8 hits.
PS50303. PUM_HD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPUM2_MOUSE
AccessioniPrimary (citable) accession number: Q80U58
Secondary accession number(s): Q80UZ9
, Q91YW4, Q925A0, Q9ERC7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: February 2, 2004
Last modified: November 2, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.