Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein CLEC16A

Gene

Clec16a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulator of mitophagy through the upstream regulation of the RNF41/NRDP1-PARK2 pathway. Mitophagy is a selective form of autophagy necessary for mitochondrial quality control. The RNF41/NRDP1-PARK2 pathway regulates autophagosome-lysosome fusion during late mitophagy. May protect RNF41/NRDP1 from proteosomal degradation, RNF41/NRDP1 which regulates proteosomal degradation of PARK2. Plays a key role in beta cells functions by regulating mitophagy/autophagy and mitochondrial health.1 Publication

GO - Biological processi

  • autophagy Source: UniProtKB-KW
  • negative regulation of macromitophagy Source: MGI
  • negative regulation of proteasomal ubiquitin-dependent protein catabolic process Source: MGI
  • positive regulation of autophagosome maturation Source: MGI
Complete GO annotation...

Keywords - Biological processi

Autophagy

Names & Taxonomyi

Protein namesi
Recommended name:
Protein CLEC16ACurated
Gene namesi
Name:Clec16aImported
Synonyms:Kiaa0350
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1921624. Clec16a.

Subcellular locationi

GO - Cellular componenti

  • endolysosome membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Lysosome, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice with pancreatic specific deletion of Clec16a are significantly hyperglycemic and have reduced basal and blunted insulin release after glucose administration. Mutant mice have normal islet architecture and beta cell mass, but beta cells show accumulation of vacuolated structures and unhealthy mitochondria (rounded mitochondria with disordered and amorphous structure). Not associated with immune infiltration and insulitis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10361036Protein CLEC16APRO_0000274477Add
BLAST

Proteomic databases

MaxQBiQ80U30.
PaxDbiQ80U30.
PeptideAtlasiQ80U30.
PRIDEiQ80U30.

PTM databases

iPTMnetiQ80U30.
PhosphoSiteiQ80U30.

Expressioni

Tissue specificityi

Ubiquitously expressed. Expressed in pancreatic islets.2 Publications

Gene expression databases

BgeeiENSMUSG00000068663.
CleanExiMM_CLEC16A.
ExpressionAtlasiQ80U30. baseline and differential.
GenevisibleiQ80U30. MM.

Interactioni

Subunit structurei

Interacts with RNF41/NRDP1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Rnf41Q8BH753EBI-9696757,EBI-7059583

Protein-protein interaction databases

BioGridi216700. 1 interaction.
IntActiQ80U30. 1 interaction.
STRINGi10090.ENSMUSP00000065423.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi828 – 91689Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the CLEC16A/gop-1 family.Curated

Phylogenomic databases

eggNOGiKOG2219. Eukaryota.
ENOG410Y0P3. LUCA.
GeneTreeiENSGT00390000013826.
HOVERGENiHBG096072.
InParanoidiQ80U30.
KOiK19513.
OMAiAQPDGKI.
PhylomeDBiQ80U30.
TreeFamiTF314293.

Family and domain databases

InterProiIPR019155. Uncharacterised_FPL.
[Graphical view]
PfamiPF09758. FPL. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80U30-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFGRSRSWVG GGHSKSSRNI HSLDHLKYLY HVLTKNTTVT EQNRNLLVET
60 70 80 90 100
IRSITEILIW GDQNDSSVFD FFLEKNMFVF FLNILRQKSG RYVCVQLLQT
110 120 130 140 150
LNILFENISH ETSLYYLLSN NYVNSIIVHK FDFSDEEIMA YYISFLKTLS
160 170 180 190 200
LKLNNHTVHF FYNEHTNDFA LYTEAIKFFN HPESMVRIAV RTITLNVYKV
210 220 230 240 250
SLDNQAMLHY IRDKTAVPYF SNLVWFIGSH VIELDNCVQT DEEHRNRGKL
260 270 280 290 300
SDLVAEHLDH LHYLNDILII NCEFLNDVLT DHLLNRLFLP LYVYSLENPD
310 320 330 340 350
KGGERPKISL PVSLYLLSQV FLIIHHAPLV NSLAEVILNG DLSETYTKPA
360 370 380 390 400
QDVPRSSAKP SIRCFIKPTE TLERSLEMNK HKGKKRMQKR PNYKNVGEEE
410 420 430 440 450
DEERGSAEDA QEDAEKTKEI EMVIMKLGKL SEVAAAGTSV QEQNTTDEEK
460 470 480 490 500
SAATNSENAQ WSRPFLDMVY HALDSPDDDY HALFVLCLLY AMSHNKGMDP
510 520 530 540 550
EKLKRIQLPV PSEAEKTTYN HLLAERLIRI MNNAAQPDGR IRLATLELSC
560 570 580 590 600
LLLKQQVLTS SGCVIKDVHL ACLEGAREES VHLVRHFYKG EEIFLDMFED
610 620 630 640 650
EYRSMTIKPM NVEYLMMDAS ILLPPTGTPL TGIDFVKRLP CGDVEKTRRA
660 670 680 690 700
IRVFFMLRSL SLQLRGEPET QLPLTREEDL IKTDDVLDLN NSDLIACTVI
710 720 730 740 750
TKDGGMVQRF LAVDIYQMSL VEPDVSRLGW GVVKFAGLLQ DMQVTGVEDD
760 770 780 790 800
SRALNITIHK PASSPHSKPF PILQATFVFS DHIRCIIAKQ RLAKGRIQAR
810 820 830 840 850
RMKMQRIAAL LDLPIQPTTE VLGFGLCSSS SSSQHLPFRF YEQCRRGSSD
860 870 880 890 900
PTVQRSVFAS VDKVPGFAVA QCINQHSSPS LSSPSPPFAS GSPGGSGSTS
910 920 930 940 950
HCDSGGSSSA PSATQSPADA PTTPEQPQPH LDQSVIGNEM DVNSKPSKNS
960 970 980 990 1000
SARSSEGETM HLSPSLLPAQ QPTISLLYED TADTLSVESL TIVPPVDPHS
1010 1020 1030
LRALSGISQL PTLPAADTET PAEGAVNPEP AEPTEH
Length:1,036
Mass (Da):116,232
Last modified:February 6, 2007 - v2
Checksum:iFC7B2FDCE9C02052
GO
Isoform 2 (identifier: Q80U30-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     201-202: Missing.

Show »
Length:1,034
Mass (Da):116,032
Checksum:iBE47F3CA5AB7B4DA
GO
Isoform 3 (identifier: Q80U30-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     201-202: Missing.
     418-418: K → KGTEGGSKSMKTSGERE
     867-898: FAVAQCINQHSSPSLSSPSPPFASGSPGGSGS → KPHLLLGTQAAFLLSPLEAFLSESRLLAALAS
     899-1036: Missing.

Show »
Length:912
Mass (Da):103,763
Checksum:iFC22CF9ECA6FDD40
GO
Isoform 4 (identifier: Q80U30-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     201-202: Missing.
     418-418: K → KGTEGGSKSMKTSGERE
     868-869: AV → HV
     870-1036: Missing.

Show »
Length:883
Mass (Da):100,785
Checksum:i2F4FD73189523326
GO
Isoform 5 (identifier: Q80U30-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-421: Missing.
     919-943: DAPTTPEQPQPHLDQSVIGNEMDVN → VRSGRKGRRRVFSLSEADSHGGHWV
     944-1036: Missing.

Show »
Length:522
Mass (Da):57,606
Checksum:i2D5293783E32E43C
GO

Sequence cautioni

The sequence BAC65537 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti56 – 561E → G in BAE33184 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 421421Missing in isoform 5. 1 PublicationVSP_022751Add
BLAST
Alternative sequencei201 – 2022Missing in isoform 2, isoform 3 and isoform 4. 1 PublicationVSP_022752
Alternative sequencei418 – 4181K → KGTEGGSKSMKTSGERE in isoform 3 and isoform 4. 1 PublicationVSP_022753
Alternative sequencei867 – 89832FAVAQ…GGSGS → KPHLLLGTQAAFLLSPLEAF LSESRLLAALAS in isoform 3. 1 PublicationVSP_022754Add
BLAST
Alternative sequencei868 – 8692AV → HV in isoform 4. 1 PublicationVSP_022755
Alternative sequencei870 – 1036167Missing in isoform 4. 1 PublicationVSP_022756Add
BLAST
Alternative sequencei899 – 1036138Missing in isoform 3. 1 PublicationVSP_022757Add
BLAST
Alternative sequencei919 – 94325DAPTT…EMDVN → VRSGRKGRRRVFSLSEADSH GGHWV in isoform 5. 1 PublicationVSP_022758Add
BLAST
Alternative sequencei944 – 103693Missing in isoform 5. 1 PublicationVSP_022759Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122255 mRNA. Translation: BAC65537.1. Different initiation.
AK016529 mRNA. Translation: BAC25487.1.
AK155314 mRNA. Translation: BAE33184.1.
AK156927 mRNA. Translation: BAE33899.1.
AK170754 mRNA. Translation: BAE42005.1.
CT010583, AC122352, AC154430 Genomic DNA. Translation: CAO77982.1.
BC138907 mRNA. Translation: AAI38908.1.
BC138909 mRNA. Translation: AAI38910.1.
CCDSiCCDS37253.1. [Q80U30-1]
CCDS57018.1. [Q80U30-2]
RefSeqiNP_001191158.1. NM_001204229.1. [Q80U30-2]
NP_808230.2. NM_177562.5. [Q80U30-1]
XP_011244330.1. XM_011246028.1. [Q80U30-4]
UniGeneiMm.159176.

Genome annotation databases

EnsembliENSMUST00000038145; ENSMUSP00000040267; ENSMUSG00000068663. [Q80U30-3]
ENSMUST00000066345; ENSMUSP00000065423; ENSMUSG00000068663. [Q80U30-1]
ENSMUST00000115823; ENSMUSP00000111489; ENSMUSG00000068663. [Q80U30-5]
ENSMUST00000115827; ENSMUSP00000111493; ENSMUSG00000068663. [Q80U30-4]
ENSMUST00000155633; ENSMUSP00000123189; ENSMUSG00000068663. [Q80U30-2]
GeneIDi74374.
KEGGimmu:74374.
UCSCiuc007ydy.2. mouse. [Q80U30-3]
uc007ydz.2. mouse. [Q80U30-4]
uc007yea.2. mouse. [Q80U30-1]
uc007yec.2. mouse. [Q80U30-5]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122255 mRNA. Translation: BAC65537.1. Different initiation.
AK016529 mRNA. Translation: BAC25487.1.
AK155314 mRNA. Translation: BAE33184.1.
AK156927 mRNA. Translation: BAE33899.1.
AK170754 mRNA. Translation: BAE42005.1.
CT010583, AC122352, AC154430 Genomic DNA. Translation: CAO77982.1.
BC138907 mRNA. Translation: AAI38908.1.
BC138909 mRNA. Translation: AAI38910.1.
CCDSiCCDS37253.1. [Q80U30-1]
CCDS57018.1. [Q80U30-2]
RefSeqiNP_001191158.1. NM_001204229.1. [Q80U30-2]
NP_808230.2. NM_177562.5. [Q80U30-1]
XP_011244330.1. XM_011246028.1. [Q80U30-4]
UniGeneiMm.159176.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi216700. 1 interaction.
IntActiQ80U30. 1 interaction.
STRINGi10090.ENSMUSP00000065423.

PTM databases

iPTMnetiQ80U30.
PhosphoSiteiQ80U30.

Proteomic databases

MaxQBiQ80U30.
PaxDbiQ80U30.
PeptideAtlasiQ80U30.
PRIDEiQ80U30.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038145; ENSMUSP00000040267; ENSMUSG00000068663. [Q80U30-3]
ENSMUST00000066345; ENSMUSP00000065423; ENSMUSG00000068663. [Q80U30-1]
ENSMUST00000115823; ENSMUSP00000111489; ENSMUSG00000068663. [Q80U30-5]
ENSMUST00000115827; ENSMUSP00000111493; ENSMUSG00000068663. [Q80U30-4]
ENSMUST00000155633; ENSMUSP00000123189; ENSMUSG00000068663. [Q80U30-2]
GeneIDi74374.
KEGGimmu:74374.
UCSCiuc007ydy.2. mouse. [Q80U30-3]
uc007ydz.2. mouse. [Q80U30-4]
uc007yea.2. mouse. [Q80U30-1]
uc007yec.2. mouse. [Q80U30-5]

Organism-specific databases

CTDi23274.
MGIiMGI:1921624. Clec16a.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG2219. Eukaryota.
ENOG410Y0P3. LUCA.
GeneTreeiENSGT00390000013826.
HOVERGENiHBG096072.
InParanoidiQ80U30.
KOiK19513.
OMAiAQPDGKI.
PhylomeDBiQ80U30.
TreeFamiTF314293.

Miscellaneous databases

ChiTaRSiClec16a. mouse.
PROiQ80U30.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000068663.
CleanExiMM_CLEC16A.
ExpressionAtlasiQ80U30. baseline and differential.
GenevisibleiQ80U30. MM.

Family and domain databases

InterProiIPR019155. Uncharacterised_FPL.
[Graphical view]
PfamiPF09758. FPL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCL16A_MOUSE
AccessioniPrimary (citable) accession number: Q80U30
Secondary accession number(s): A6X932
, B2RSL3, Q3TCF2, Q3U0F5, Q3U2F6, Q8CEQ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: February 6, 2007
Last modified: September 7, 2016
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.