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Protein

Disheveled-associated activator of morphogenesis 2

Gene

Daam2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GO - Biological processi

  • actin cytoskeleton organization Source: InterPro
  • determination of left/right symmetry Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Disheveled-associated activator of morphogenesis 2
Gene namesi
Name:Daam2
Synonyms:Kiaa0381
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1923691. Daam2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001949101 – 1115Disheveled-associated activator of morphogenesis 2Add BLAST1115

Proteomic databases

PaxDbiQ80U19.
PeptideAtlasiQ80U19.
PRIDEiQ80U19.

PTM databases

iPTMnetiQ80U19.
PhosphoSitePlusiQ80U19.
SwissPalmiQ80U19.

Expressioni

Tissue specificityi

In early embryogenesis, expression is confined to embryonic ectoderm. Highly dynamic expression in later stages of gastrulation. In early somite stages, detected in posterior node and persists until 9-10 somites have developed when expression is concentrated in the chordoneural hinge. During organogenesis, expressed in the CNS, PNS, liver primordia, limb buds and genital tubercle.2 Publications

Gene expression databases

BgeeiENSMUSG00000040260.
GenevisibleiQ80U19. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000052085.

Structurei

3D structure databases

ProteinModelPortaliQ80U19.
SMRiQ80U19.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 416GBD/FH3PROSITE-ProRule annotationAdd BLAST377
Domaini518 – 694FH1Add BLAST177
Domaini595 – 1042FH2PROSITE-ProRule annotationAdd BLAST448
Domaini1065 – 1095DADPROSITE-ProRule annotationAdd BLAST31

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili434 – 515Sequence analysisAdd BLAST82

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi518 – 604Pro-richAdd BLAST87

Domaini

The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments (By similarity).By similarity

Sequence similaritiesi

Belongs to the formin homology family.Curated
Contains 1 DAD (diaphanous autoregulatory) domain.PROSITE-ProRule annotation
Contains 1 FH2 (formin homology 2) domain.PROSITE-ProRule annotation
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1922. Eukaryota.
ENOG410XT5Z. LUCA.
GeneTreeiENSGT00760000118986.
HOGENOMiHOG000237318.
HOVERGENiHBG101333.
InParanoidiQ80U19.
KOiK04512.
OMAiGAPPCFG.
OrthoDBiEOG091G02QJ.
TreeFamiTF314602.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR014767. DAD_dom.
IPR015425. FH2_Formin.
IPR010472. FH3_dom.
IPR014768. GBD/FH3_dom.
IPR010473. GTPase-bd.
[Graphical view]
PfamiPF06367. Drf_FH3. 1 hit.
PF06371. Drf_GBD. 1 hit.
PF02181. FH2. 1 hit.
[Graphical view]
SMARTiSM01139. Drf_FH3. 1 hit.
SM01140. Drf_GBD. 1 hit.
SM00498. FH2. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS51231. DAD. 1 hit.
PS51444. FH2. 1 hit.
PS51232. GBD_FH3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80U19-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALRKRSPHG LGFLCCFGGS DLPEIDLRDS HPLQYLEFSG PIPNPEELNV
60 70 80 90 100
RFAELVDELD LTDKNREAVF ALPPEKKWQI YCSKRKEQED PNKLATSWPE
110 120 130 140 150
YYIDRINAMA AMQNLYETED EETDKRNQVV EDLKTALRTQ PMRFVTRFID
160 170 180 190 200
LEGLTCLLNF LRGMDHTTCE SRIHTSLIGC IKALMNNSQG RAHVLAQPEA
210 220 230 240 250
ISIIAQSLRT ENSKTKVAVL EILGAVCLVP GGHKKVLQAM LHYQAYAAER
260 270 280 290 300
TRFQTLLNEL DRSLGRYRDE VNLKTAIMSF INAVLNAGAG EDNLEFRLHL
310 320 330 340 350
RYEFLMLGIQ PVIDKLRQHE NAILDKHLDF FEMVRNEDDL ELARRFDMVH
360 370 380 390 400
IDTKSASQMF ELIHKKLKHT EAYPCLLSVL HHCLQMPYKR NGGYFQQWQL
410 420 430 440 450
LDRILQQIVL QDERGVDPDL APLENFNVKN IVNMLINENE VKQWRDQAEK
460 470 480 490 500
FRKEHMELMS RLERKERECE TKTLEKEEMM RTLNKMKDKL ARESQELRQA
510 520 530 540 550
RGQVAELVAR HNESSTGPVS SPPPPGGPLT LSSSRTTNDL PPPPPPLPFD
560 570 580 590 600
SCPPPPAPPL PPGGPPIPPG APPCFSSGPP PSHDPFSSNE APLRKKRIPQ
610 620 630 640 650
PSHPLKSFNW VKLNEERVSG TVWNEIDDSQ VFRILDLEDF EKMFSAYQRH
660 670 680 690 700
QACMQEGPQR ERGNVRDGGA ASRPLPAVEA SAHRTEKASR SMVSATGAKK
710 720 730 740 750
ELGSTEDIYI TSRKVKELSV IDGRRAQNCI ILLSKLKLSN DEIRQAILRM
760 770 780 790 800
DEQEDLAKDM LEQLLKFIPE KSDIDLLEEH KHEIERMARA DRFLYEMSRI
810 820 830 840 850
DHYQQRLQAL FFKKKFQERL AEAKPKVEAI LLASRELTLS QRLKQMLEVV
860 870 880 890 900
LAIGNFMNKG QRGGAYGFRV ASLNKIADTK SSIDRNISLL HYLIMILEKH
910 920 930 940 950
FPDILNMPSE LKHLSEAAKV NLAELEKEVS ILRRGLRAVE VELEYQRHQA
960 970 980 990 1000
RDPNDKFVPV MSDFITVSSF SFSELEDQLN EARDKFAKAL THFGEQESKM
1010 1020 1030 1040 1050
QPDEFFGIFD TFLQAFLEAR QDLEAMRRRK EEDERRARME FMLKEQREKE
1060 1070 1080 1090 1100
RWQRQRKVLA GGALEESGEF DDLVSALRSG EVFDKDLSKF KRNRKRPGSQ
1110
VPEVTRERAI NRLNY
Length:1,115
Mass (Da):128,370
Last modified:July 27, 2011 - v4
Checksum:iEC327BF7CFD5A889
GO
Isoform 2 (identifier: Q80U19-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     516-524: TGPVSSPPP → VRGHHPHPI
     525-1115: Missing.

Note: No experimental confirmation available.
Show »
Length:524
Mass (Da):60,980
Checksum:i1D9C0DBB885F3882
GO

Sequence cautioni

The sequence BAC65548 differs from that shown. Partially unspliced pre-RNA.Curated
The sequence BAC65548 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti994G → R in AAR05119 (PubMed:15533824).Curated1
Sequence conflicti997E → D in AAR05119 (PubMed:15533824).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008005516 – 524TGPVSSPPP → VRGHHPHPI in isoform 2. 1 Publication9
Alternative sequenceiVSP_008006525 – 1115Missing in isoform 2. 1 PublicationAdd BLAST591

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY426536 mRNA. Translation: AAR05119.1.
AK122266 Transcribed RNA. Translation: BAC65548.2. Sequence problems.
AC124748 Genomic DNA. No translation available.
AC165258 Genomic DNA. No translation available.
BC050043 mRNA. Translation: AAH50043.1.
CCDSiCCDS37648.1. [Q80U19-1]
RefSeqiNP_001008232.2. NM_001008231.2. [Q80U19-1]
UniGeneiMm.211275.

Genome annotation databases

EnsembliENSMUST00000057610; ENSMUSP00000052085; ENSMUSG00000040260. [Q80U19-1]
GeneIDi76441.
KEGGimmu:76441.
UCSCiuc008cyl.1. mouse. [Q80U19-1]
uc008cyn.1. mouse. [Q80U19-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY426536 mRNA. Translation: AAR05119.1.
AK122266 Transcribed RNA. Translation: BAC65548.2. Sequence problems.
AC124748 Genomic DNA. No translation available.
AC165258 Genomic DNA. No translation available.
BC050043 mRNA. Translation: AAH50043.1.
CCDSiCCDS37648.1. [Q80U19-1]
RefSeqiNP_001008232.2. NM_001008231.2. [Q80U19-1]
UniGeneiMm.211275.

3D structure databases

ProteinModelPortaliQ80U19.
SMRiQ80U19.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000052085.

PTM databases

iPTMnetiQ80U19.
PhosphoSitePlusiQ80U19.
SwissPalmiQ80U19.

Proteomic databases

PaxDbiQ80U19.
PeptideAtlasiQ80U19.
PRIDEiQ80U19.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000057610; ENSMUSP00000052085; ENSMUSG00000040260. [Q80U19-1]
GeneIDi76441.
KEGGimmu:76441.
UCSCiuc008cyl.1. mouse. [Q80U19-1]
uc008cyn.1. mouse. [Q80U19-2]

Organism-specific databases

CTDi23500.
MGIiMGI:1923691. Daam2.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1922. Eukaryota.
ENOG410XT5Z. LUCA.
GeneTreeiENSGT00760000118986.
HOGENOMiHOG000237318.
HOVERGENiHBG101333.
InParanoidiQ80U19.
KOiK04512.
OMAiGAPPCFG.
OrthoDBiEOG091G02QJ.
TreeFamiTF314602.

Miscellaneous databases

PROiQ80U19.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040260.
GenevisibleiQ80U19. MM.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR014767. DAD_dom.
IPR015425. FH2_Formin.
IPR010472. FH3_dom.
IPR014768. GBD/FH3_dom.
IPR010473. GTPase-bd.
[Graphical view]
PfamiPF06367. Drf_FH3. 1 hit.
PF06371. Drf_GBD. 1 hit.
PF02181. FH2. 1 hit.
[Graphical view]
SMARTiSM01139. Drf_FH3. 1 hit.
SM01140. Drf_GBD. 1 hit.
SM00498. FH2. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS51231. DAD. 1 hit.
PS51444. FH2. 1 hit.
PS51232. GBD_FH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDAAM2_MOUSE
AccessioniPrimary (citable) accession number: Q80U19
Secondary accession number(s): E9QNU9, Q6TAB7, Q810J5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 115 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.