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Protein

Disheveled-associated activator of morphogenesis 2

Gene

Daam2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Key regulator of the Wnt signaling pathway, which is required for various processes during development, such as dorsal patterning, determination of left/right symmetry or myelination in the central nervous system (PubMed:22227309, PubMed:24091014, PubMed:25754822). Acts downstream of Wnt ligands and upstream of beta-catenin (CTNNB1) (PubMed:22227309, PubMed:25754822). Required for canonical Wnt signaling pathway during patterning in the dorsal spinal cord by promoting the aggregation of Disheveled (Dvl) complexes, thereby clustering and formation of Wnt receptor signalosomes and potentiating Wnt activity (PubMed:22227309). During dorsal patterning of the spinal cord, inhibits oligodendrocytes differentiation via interaction with PIP5K1A (PubMed:25754822). Also regulates non-canonical Wnt signaling pathway (PubMed:24091014). Acts downstream of PITX2 in the developing gut and is required for left/right asymmetry within dorsal mesentery: affects mesenchymal condensation by lengthening cadherin-based junctions through WNT5A and non-canonical Wnt signaling, inducing polarized condensation in the left dorsal mesentery necessary to initiate gut rotation (PubMed:24091014). Together with DAAM1, required for myocardial maturation and sarcomere assembly (PubMed:26526197).4 Publications

GO - Molecular functioni

GO - Biological processi

  • actin cytoskeleton organization Source: InterPro
  • determination of left/right symmetry Source: MGI
  • dorsal spinal cord development Source: UniProtKB
  • negative regulation of oligodendrocyte differentiation Source: UniProtKB
  • positive regulation of canonical Wnt signaling pathway Source: UniProtKB
  • regulation of canonical Wnt signaling pathway Source: UniProtKB
  • regulation of non-canonical Wnt signaling pathway Source: UniProtKB
  • Wnt signaling pathway Source: UniProtKB-KW

Keywordsi

Biological processWnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Disheveled-associated activator of morphogenesis 21 Publication
Gene namesi
Name:Daam21 PublicationImported
Synonyms:Kiaa03811 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1923691 Daam2

Pathology & Biotechi

Disruption phenotypei

Conditional knockout mice lacking Daam2 in myocardial cells do not show any heart defects. Conditional knockout mice lacking Daam1 and Daam2 in myocardial cells show cardiomyopathy, which is stronger than with a single Daam1 deletion.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001949101 – 1115Disheveled-associated activator of morphogenesis 2Add BLAST1115

Proteomic databases

PaxDbiQ80U19
PeptideAtlasiQ80U19
PRIDEiQ80U19

PTM databases

iPTMnetiQ80U19
PhosphoSitePlusiQ80U19
SwissPalmiQ80U19

Expressioni

Tissue specificityi

In early embryogenesis, expression is confined to embryonic ectoderm. Highly dynamic expression in later stages of gastrulation. In early somite stages, detected in posterior node and persists until 9-10 somites have developed when expression is concentrated in the chordoneural hinge. During organogenesis, expressed in the CNS, PNS, liver primordia, limb buds and genital tubercle.2 Publications

Developmental stagei

Expressed in the embryonic myocardium: not expressed in the myocardium at E9.5 but is present in epicardial cells. At E10.5, expressed in the mesenchyme surrounding the ventral foregut and in regions enriched in cardiac progenitors, as well as the epicardium and lining of the pericardial cavity. By E12.5, expressed throughout the myocardium and ventricular trabeculae.1 Publication

Gene expression databases

BgeeiENSMUSG00000040260
GenevisibleiQ80U19 MM

Interactioni

Subunit structurei

Interacts with DVL3.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000052085

Structurei

3D structure databases

ProteinModelPortaliQ80U19
SMRiQ80U19
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 416GBD/FH3PROSITE-ProRule annotationAdd BLAST377
Domaini518 – 694FH1Add BLAST177
Domaini595 – 1042FH2PROSITE-ProRule annotationAdd BLAST448
Domaini1065 – 1095DADPROSITE-ProRule annotationAdd BLAST31

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili434 – 515Sequence analysisAdd BLAST82

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi518 – 604Pro-richAdd BLAST87

Domaini

The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments (By similarity).By similarity

Sequence similaritiesi

Belongs to the formin homology family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1922 Eukaryota
ENOG410XT5Z LUCA
GeneTreeiENSGT00760000118986
HOGENOMiHOG000237318
HOVERGENiHBG101333
InParanoidiQ80U19
KOiK04512
OMAiIMILEKH
OrthoDBiEOG091G02QJ
TreeFamiTF314602

Family and domain databases

Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR014767 DAD_dom
IPR015425 FH2_Formin
IPR010472 FH3_dom
IPR014768 GBD/FH3_dom
IPR010473 GTPase-bd
PfamiView protein in Pfam
PF06367 Drf_FH3, 1 hit
PF06371 Drf_GBD, 1 hit
PF02181 FH2, 1 hit
SMARTiView protein in SMART
SM01139 Drf_FH3, 1 hit
SM01140 Drf_GBD, 1 hit
SM00498 FH2, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS51231 DAD, 1 hit
PS51444 FH2, 1 hit
PS51232 GBD_FH3, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80U19-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALRKRSPHG LGFLCCFGGS DLPEIDLRDS HPLQYLEFSG PIPNPEELNV
60 70 80 90 100
RFAELVDELD LTDKNREAVF ALPPEKKWQI YCSKRKEQED PNKLATSWPE
110 120 130 140 150
YYIDRINAMA AMQNLYETED EETDKRNQVV EDLKTALRTQ PMRFVTRFID
160 170 180 190 200
LEGLTCLLNF LRGMDHTTCE SRIHTSLIGC IKALMNNSQG RAHVLAQPEA
210 220 230 240 250
ISIIAQSLRT ENSKTKVAVL EILGAVCLVP GGHKKVLQAM LHYQAYAAER
260 270 280 290 300
TRFQTLLNEL DRSLGRYRDE VNLKTAIMSF INAVLNAGAG EDNLEFRLHL
310 320 330 340 350
RYEFLMLGIQ PVIDKLRQHE NAILDKHLDF FEMVRNEDDL ELARRFDMVH
360 370 380 390 400
IDTKSASQMF ELIHKKLKHT EAYPCLLSVL HHCLQMPYKR NGGYFQQWQL
410 420 430 440 450
LDRILQQIVL QDERGVDPDL APLENFNVKN IVNMLINENE VKQWRDQAEK
460 470 480 490 500
FRKEHMELMS RLERKERECE TKTLEKEEMM RTLNKMKDKL ARESQELRQA
510 520 530 540 550
RGQVAELVAR HNESSTGPVS SPPPPGGPLT LSSSRTTNDL PPPPPPLPFD
560 570 580 590 600
SCPPPPAPPL PPGGPPIPPG APPCFSSGPP PSHDPFSSNE APLRKKRIPQ
610 620 630 640 650
PSHPLKSFNW VKLNEERVSG TVWNEIDDSQ VFRILDLEDF EKMFSAYQRH
660 670 680 690 700
QACMQEGPQR ERGNVRDGGA ASRPLPAVEA SAHRTEKASR SMVSATGAKK
710 720 730 740 750
ELGSTEDIYI TSRKVKELSV IDGRRAQNCI ILLSKLKLSN DEIRQAILRM
760 770 780 790 800
DEQEDLAKDM LEQLLKFIPE KSDIDLLEEH KHEIERMARA DRFLYEMSRI
810 820 830 840 850
DHYQQRLQAL FFKKKFQERL AEAKPKVEAI LLASRELTLS QRLKQMLEVV
860 870 880 890 900
LAIGNFMNKG QRGGAYGFRV ASLNKIADTK SSIDRNISLL HYLIMILEKH
910 920 930 940 950
FPDILNMPSE LKHLSEAAKV NLAELEKEVS ILRRGLRAVE VELEYQRHQA
960 970 980 990 1000
RDPNDKFVPV MSDFITVSSF SFSELEDQLN EARDKFAKAL THFGEQESKM
1010 1020 1030 1040 1050
QPDEFFGIFD TFLQAFLEAR QDLEAMRRRK EEDERRARME FMLKEQREKE
1060 1070 1080 1090 1100
RWQRQRKVLA GGALEESGEF DDLVSALRSG EVFDKDLSKF KRNRKRPGSQ
1110
VPEVTRERAI NRLNY
Length:1,115
Mass (Da):128,370
Last modified:July 27, 2011 - v4
Checksum:iEC327BF7CFD5A889
GO
Isoform 2 (identifier: Q80U19-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     516-524: TGPVSSPPP → VRGHHPHPI
     525-1115: Missing.

Note: No experimental confirmation available.
Show »
Length:524
Mass (Da):60,980
Checksum:i1D9C0DBB885F3882
GO

Sequence cautioni

The sequence BAC65548 differs from that shown. Partially unspliced pre-RNA.Curated
The sequence BAC65548 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti994G → R in AAR05119 (PubMed:15533824).Curated1
Sequence conflicti997E → D in AAR05119 (PubMed:15533824).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008005516 – 524TGPVSSPPP → VRGHHPHPI in isoform 2. 1 Publication9
Alternative sequenceiVSP_008006525 – 1115Missing in isoform 2. 1 PublicationAdd BLAST591

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY426536 mRNA Translation: AAR05119.1
AK122266 Transcribed RNA Translation: BAC65548.2 Sequence problems.
AC124748 Genomic DNA No translation available.
AC165258 Genomic DNA No translation available.
BC050043 mRNA Translation: AAH50043.1
CCDSiCCDS37648.1 [Q80U19-1]
RefSeqiNP_001008232.2, NM_001008231.2 [Q80U19-1]
UniGeneiMm.211275

Genome annotation databases

EnsembliENSMUST00000057610; ENSMUSP00000052085; ENSMUSG00000040260 [Q80U19-1]
ENSMUST00000224595; ENSMUSP00000153095; ENSMUSG00000040260 [Q80U19-2]
GeneIDi76441
KEGGimmu:76441
UCSCiuc008cyl.1 mouse [Q80U19-1]
uc008cyn.1 mouse [Q80U19-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDAAM2_MOUSE
AccessioniPrimary (citable) accession number: Q80U19
Secondary accession number(s): E9QNU9, Q6TAB7, Q810J5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: July 27, 2011
Last modified: April 25, 2018
This is version 124 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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