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Q80TZ3 (AUXI_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative tyrosine-protein phosphatase auxilin

EC=3.1.3.48
Alternative name(s):
DnaJ homolog subfamily C member 6
Gene names
Name:Dnajc6
Synonyms:Kiaa0473
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length938 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Recruits HSPA8/HSC70 to clathrin-coated vesicles and promotes uncoating of clathrin-coated vesicles. Plays a role in clathrin-mediated endocytosis in neurons. Ref.5

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Subunit structure

Interacts with HSPA8/HSC70. Interacts with CLTC. Interacts with AP2A2 By similarity.

Disruption phenotype

Mice have a high rate of early postnatal mortality, although surviving pups have a normal life span despite decreased body weight. Knockout animals have impaired synaptic vesicle recycling, with an increased number of clathrin-coated vesicles, and impaired clathrin-mediated endocytosis of synaptic vesicles in neuronal culture. There is an up-regulation of Gak, but this does not fully compensate for the lack of the protein. The brains from mutant mice do not display alterations in substantia nigra morphology or dopamine transporter abundance or distribution, in agreement with the lack of gait or movement abnormalities in the mutant animals. Ref.5

Sequence similarities

Contains 1 C2 tensin-type domain.

Contains 1 J domain.

Contains 1 phosphatase tensin-type domain.

Sequence caution

The sequence AAH60734.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q80TZ3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q80TZ3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-38: Missing.
Isoform 3 (identifier: Q80TZ3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: MTNPKSGVAESAGLACSRAAAGENRMKDSENK → MSLLGSYRKK...PHRASTTDSS

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 938938Putative tyrosine-protein phosphatase auxilin
PRO_0000244517

Regions

Repeat61 – 6441
Repeat65 – 6842
Repeat69 – 7243
Domain80 – 247168Phosphatase tensin-type
Domain253 – 391139C2 tensin-type
Domain874 – 93865J
Region61 – 72123 X 4 AA approximate tandem repeats
Motif434 – 4429SH3-binding Potential
Compositional bias491 – 785295Pro-rich

Sites

Active site1891Phosphocysteine intermediate Potential

Amino acid modifications

Modified residue5951Phosphoserine By similarity

Natural variations

Alternative sequence1 – 3838Missing in isoform 2.
VSP_019582
Alternative sequence1 – 3232MTNPK…DSENK → MSLLGSYRKKTSSDGYESLQ LVDSHGDSSARGAAAGTQRA TAGAVRSPARQPPHRASTTD SS in isoform 3.
VSP_019583

Experimental info

Sequence conflict4421P → T in BAC65575. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 27, 2006. Version 2.
Checksum: 3DFB7D9275BEF3F6

FASTA938102,299
        10         20         30         40         50         60 
MTNPKSGVAE SAGLACSRAA AGENRMKDSE NKGASSPDME PSYGGGLFDM VKGGAGRLFS 

        70         80         90        100        110        120 
NLKDNLKDTL KDTSSRVIQS VSSYTKGDLD FTYVTSRIIV MSFPVDSVDI GFRNQVDDIR 

       130        140        150        160        170        180 
SFLDSRHLDH YTVYNLSPKS YRTAKFHSRV SECSWPIRQA PSLHNLFAVC RNMYNWLLQN 

       190        200        210        220        230        240 
PKNVCVVHCL DGRAASSILV GAMFIFCNLY STPGPAVRLL YAKRPGIGLS PSHRRYLGYM 

       250        260        270        280        290        300 
CDLLADKPYR PHFKPLTIKA ITVSPVPFFN KQRNGCRPYC DVLIGETKIY STCTDFERMK 

       310        320        330        340        350        360 
EYRVQDGKIF IPLNITVQGD VIVSMYHLRS TIGSRLQAKV TNTQIFQLQF HSGFIPLDTT 

       370        380        390        400        410        420 
VLKFTKPELD ACDVPEKYPQ LFQVTLDIEV QPQDKVIDLT PPWEHYCTKD VNPSILFSSQ 

       430        440        450        460        470        480 
QEHQDTLALG GQAPADLPPD HPRNLGQGGF FASLCWQDQK SEKSRCEEDH AALVNQESEQ 

       490        500        510        520        530        540 
SDDELLTLSS PHGNAEGDKP HGAKKPGKKQ QEPAAPPPPE EVDLLGLEGS DVSTNFSSLA 

       550        560        570        580        590        600 
APPSNSELLS DLFGGVGATG PAQAGQAGVE DVFHPSGPVS AQSTPRRTAT SASASPTLRV 

       610        620        630        640        650        660 
GEGATFDPFG APAKPPGQDL LGSFLNTSSA SSDPFLQPTR SPSPTVHASS TPAVNIQPDI 

       670        680        690        700        710        720 
AGGWDWHTKP GGFGMGSKSA ATSPTGSSHG TPTHQSKPQT LDPFADLGTL GSSSFASKPT 

       730        740        750        760        770        780 
TPTGLGGGFP PLSSPQKASP QPMGGGWQQP AGYNWQQTQS KPQSSMPHSS PQNRPNYNVS 

       790        800        810        820        830        840 
FSAMPAGQSE RGKGSTNLEG KQKAADFEDL LSSQGFNAHK DKKGPRTIAE MRKEEMAKEM 

       850        860        870        880        890        900 
DPEKLKILEW IEGKERNIRA LLSTMHTVLW AGETKWKPVG MADLVTPEQV KKVYRRAVLV 

       910        920        930 
VHPDKATGQP YEQYAKMIFM ELNDAWSEFE NQGQKPLY 

« Hide

Isoform 2 [UniParc].

Checksum: 9D35C6511ECD7BE5
Show »

FASTA90098,521
Isoform 3 [UniParc].

Checksum: 83C4E04C24405E01
Show »

FASTA968105,409

References

« Hide 'large scale' references
[1]"Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Brain, Hippocampus and Medulla oblongata.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
Strain: C57BL/6.
Tissue: Brain.
[5]"Endocytosis and clathrin-uncoating defects at synapses of auxilin knockout mice."
Yim Y.I., Sun T., Wu L.G., Raimondi A., De Camilli P., Eisenberg E., Greene L.E.
Proc. Natl. Acad. Sci. U.S.A. 107:4412-4417(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE, FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK122293 mRNA. Translation: BAC65575.1.
AK049935 mRNA. Translation: BAC33992.1.
AK147570 mRNA. Translation: BAE28000.1.
AK147637 mRNA. Translation: BAE28039.1.
AK163657 mRNA. Translation: BAE37443.1.
BX323551 Genomic DNA. Translation: CAM16148.1.
BC060734 mRNA. Translation: AAH60734.1. Different initiation.
BC064460 mRNA. Translation: AAH64460.1.
CCDSCCDS18395.1. [Q80TZ3-2]
CCDS51237.1. [Q80TZ3-1]
CCDS51238.1. [Q80TZ3-3]
RefSeqNP_001158055.1. NM_001164583.1. [Q80TZ3-3]
NP_001158056.1. NM_001164584.1. [Q80TZ3-1]
NP_001158057.1. NM_001164585.1. [Q80TZ3-2]
NP_940804.1. NM_198412.2. [Q80TZ3-2]
UniGeneMm.76494.

3D structure databases

ProteinModelPortalQ80TZ3.
SMRQ80TZ3. Positions 81-427, 762-938.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ80TZ3. 4 interactions.
MINTMINT-4114665.

PTM databases

PhosphoSiteQ80TZ3.

Proteomic databases

MaxQBQ80TZ3.
PaxDbQ80TZ3.
PRIDEQ80TZ3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000038207; ENSMUSP00000044251; ENSMUSG00000028528. [Q80TZ3-1]
ENSMUST00000094953; ENSMUSP00000092560; ENSMUSG00000028528. [Q80TZ3-2]
ENSMUST00000106929; ENSMUSP00000102542; ENSMUSG00000028528. [Q80TZ3-2]
ENSMUST00000106930; ENSMUSP00000102543; ENSMUSG00000028528. [Q80TZ3-2]
ENSMUST00000106933; ENSMUSP00000102546; ENSMUSG00000028528. [Q80TZ3-3]
GeneID72685.
KEGGmmu:72685.
UCSCuc008tvq.2. mouse. [Q80TZ3-1]
uc008tvt.1. mouse. [Q80TZ3-3]

Organism-specific databases

CTD9829.
MGIMGI:1919935. Dnajc6.
RougeSearch...

Phylogenomic databases

eggNOGNOG269956.
GeneTreeENSGT00620000087779.
HOGENOMHOG000034235.
HOVERGENHBG004322.
InParanoidB1B0B9.
KOK09526.
OMALCWQDQK.
OrthoDBEOG7WT40V.
PhylomeDBQ80TZ3.
TreeFamTF105165.

Gene expression databases

ArrayExpressQ80TZ3.
BgeeQ80TZ3.
CleanExMM_DNAJC6.
GenevestigatorQ80TZ3.

Family and domain databases

Gene3D1.10.287.110. 1 hit.
3.90.190.10. 1 hit.
InterProIPR000008. C2_dom.
IPR001623. DnaJ_domain.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR014020. Tensin_C2-dom.
IPR029023. Tensin_lipid_phosphatase_dom.
[Graphical view]
PfamPF00226. DnaJ. 1 hit.
PF10409. PTEN_C2. 1 hit.
[Graphical view]
SMARTSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMSSF46565. SSF46565. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF52799. SSF52799. 1 hit.
PROSITEPS51182. C2_TENSIN. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51181. PPASE_TENSIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio336731.
PROQ80TZ3.
SOURCESearch...

Entry information

Entry nameAUXI_MOUSE
AccessionPrimary (citable) accession number: Q80TZ3
Secondary accession number(s): B1B0B9, Q6P2K9, Q8C7L9
Entry history
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: June 27, 2006
Last modified: July 9, 2014
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot