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Q80TZ3

- AUXI_MOUSE

UniProt

Q80TZ3 - AUXI_MOUSE

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Protein

Putative tyrosine-protein phosphatase auxilin

Gene

Dnajc6

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Recruits HSPA8/HSC70 to clathrin-coated vesicles and promotes uncoating of clathrin-coated vesicles. Plays a role in clathrin-mediated endocytosis in neurons.1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei189 – 1891Phosphocysteine intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. protein tyrosine phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. clathrin coat disassembly Source: MGI
  2. regulation of clathrin-mediated endocytosis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Chaperone, Hydrolase, Protein phosphatase

Enzyme and pathway databases

ReactomeiREACT_231722. Lysosome Vesicle Biogenesis.
REACT_232671. Golgi Associated Vesicle Biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative tyrosine-protein phosphatase auxilin (EC:3.1.3.48)
Alternative name(s):
DnaJ homolog subfamily C member 6
Gene namesi
Name:Dnajc6
Synonyms:Kiaa0473
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:1919935. Dnajc6.

Subcellular locationi

GO - Cellular componenti

  1. synapse Source: MGI
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Mice have a high rate of early postnatal mortality, although surviving pups have a normal life span despite decreased body weight. Knockout animals have impaired synaptic vesicle recycling, with an increased number of clathrin-coated vesicles, and impaired clathrin-mediated endocytosis of synaptic vesicles in neuronal culture. There is an up-regulation of Gak, but this does not fully compensate for the lack of the protein. The brains from mutant mice do not display alterations in substantia nigra morphology or dopamine transporter abundance or distribution, in agreement with the lack of gait or movement abnormalities in the mutant animals.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 938938Putative tyrosine-protein phosphatase auxilinPRO_0000244517Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei595 – 5951PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ80TZ3.
PaxDbiQ80TZ3.
PRIDEiQ80TZ3.

PTM databases

PhosphoSiteiQ80TZ3.

Expressioni

Gene expression databases

BgeeiQ80TZ3.
CleanExiMM_DNAJC6.
ExpressionAtlasiQ80TZ3. baseline and differential.
GenevestigatoriQ80TZ3.

Interactioni

Subunit structurei

Interacts with HSPA8/HSC70. Interacts with CLTC. Interacts with AP2A2 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ80TZ3. 4 interactions.
MINTiMINT-4114665.

Structurei

3D structure databases

ProteinModelPortaliQ80TZ3.
SMRiQ80TZ3. Positions 81-427, 762-938.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati61 – 6441
Repeati65 – 6842
Repeati69 – 7243
Domaini80 – 247168Phosphatase tensin-typePROSITE-ProRule annotationAdd
BLAST
Domaini253 – 391139C2 tensin-typePROSITE-ProRule annotationAdd
BLAST
Domaini874 – 93865JPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni61 – 72123 X 4 AA approximate tandem repeatsAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi434 – 4429SH3-bindingSequence Analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi491 – 785295Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 C2 tensin-type domain.PROSITE-ProRule annotation
Contains 1 J domain.PROSITE-ProRule annotation
Contains 1 phosphatase tensin-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH3-binding

Phylogenomic databases

eggNOGiNOG269956.
GeneTreeiENSGT00760000119113.
HOGENOMiHOG000034235.
HOVERGENiHBG004322.
InParanoidiQ80TZ3.
KOiK09526.
OMAiLCWQDQK.
OrthoDBiEOG7WT40V.
PhylomeDBiQ80TZ3.
TreeFamiTF105165.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR000008. C2_dom.
IPR001623. DnaJ_domain.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR014020. Tensin_C2-dom.
IPR029023. Tensin_lipid_phosphatase_dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF10409. PTEN_C2. 1 hit.
[Graphical view]
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF52799. SSF52799. 1 hit.
PROSITEiPS51182. C2_TENSIN. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51181. PPASE_TENSIN. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q80TZ3-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTNPKSGVAE SAGLACSRAA AGENRMKDSE NKGASSPDME PSYGGGLFDM
60 70 80 90 100
VKGGAGRLFS NLKDNLKDTL KDTSSRVIQS VSSYTKGDLD FTYVTSRIIV
110 120 130 140 150
MSFPVDSVDI GFRNQVDDIR SFLDSRHLDH YTVYNLSPKS YRTAKFHSRV
160 170 180 190 200
SECSWPIRQA PSLHNLFAVC RNMYNWLLQN PKNVCVVHCL DGRAASSILV
210 220 230 240 250
GAMFIFCNLY STPGPAVRLL YAKRPGIGLS PSHRRYLGYM CDLLADKPYR
260 270 280 290 300
PHFKPLTIKA ITVSPVPFFN KQRNGCRPYC DVLIGETKIY STCTDFERMK
310 320 330 340 350
EYRVQDGKIF IPLNITVQGD VIVSMYHLRS TIGSRLQAKV TNTQIFQLQF
360 370 380 390 400
HSGFIPLDTT VLKFTKPELD ACDVPEKYPQ LFQVTLDIEV QPQDKVIDLT
410 420 430 440 450
PPWEHYCTKD VNPSILFSSQ QEHQDTLALG GQAPADLPPD HPRNLGQGGF
460 470 480 490 500
FASLCWQDQK SEKSRCEEDH AALVNQESEQ SDDELLTLSS PHGNAEGDKP
510 520 530 540 550
HGAKKPGKKQ QEPAAPPPPE EVDLLGLEGS DVSTNFSSLA APPSNSELLS
560 570 580 590 600
DLFGGVGATG PAQAGQAGVE DVFHPSGPVS AQSTPRRTAT SASASPTLRV
610 620 630 640 650
GEGATFDPFG APAKPPGQDL LGSFLNTSSA SSDPFLQPTR SPSPTVHASS
660 670 680 690 700
TPAVNIQPDI AGGWDWHTKP GGFGMGSKSA ATSPTGSSHG TPTHQSKPQT
710 720 730 740 750
LDPFADLGTL GSSSFASKPT TPTGLGGGFP PLSSPQKASP QPMGGGWQQP
760 770 780 790 800
AGYNWQQTQS KPQSSMPHSS PQNRPNYNVS FSAMPAGQSE RGKGSTNLEG
810 820 830 840 850
KQKAADFEDL LSSQGFNAHK DKKGPRTIAE MRKEEMAKEM DPEKLKILEW
860 870 880 890 900
IEGKERNIRA LLSTMHTVLW AGETKWKPVG MADLVTPEQV KKVYRRAVLV
910 920 930
VHPDKATGQP YEQYAKMIFM ELNDAWSEFE NQGQKPLY
Length:938
Mass (Da):102,299
Last modified:June 27, 2006 - v2
Checksum:i3DFB7D9275BEF3F6
GO
Isoform 2 (identifier: Q80TZ3-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-38: Missing.

Show »
Length:900
Mass (Da):98,521
Checksum:i9D35C6511ECD7BE5
GO
Isoform 3 (identifier: Q80TZ3-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: MTNPKSGVAESAGLACSRAAAGENRMKDSENK → MSLLGSYRKK...PHRASTTDSS

Show »
Length:968
Mass (Da):105,409
Checksum:i83C4E04C24405E01
GO

Sequence cautioni

The sequence AAH60734.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti442 – 4421P → T in BAC65575. (PubMed:12693553)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 3838Missing in isoform 2. 2 PublicationsVSP_019582Add
BLAST
Alternative sequencei1 – 3232MTNPK…DSENK → MSLLGSYRKKTSSDGYESLQ LVDSHGDSSARGAAAGTQRA TAGAVRSPARQPPHRASTTD SS in isoform 3. 1 PublicationVSP_019583Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122293 mRNA. Translation: BAC65575.1.
AK049935 mRNA. Translation: BAC33992.1.
AK147570 mRNA. Translation: BAE28000.1.
AK147637 mRNA. Translation: BAE28039.1.
AK163657 mRNA. Translation: BAE37443.1.
BX323551 Genomic DNA. Translation: CAM16148.1.
BC060734 mRNA. Translation: AAH60734.1. Different initiation.
BC064460 mRNA. Translation: AAH64460.1.
CCDSiCCDS18395.1. [Q80TZ3-2]
CCDS51237.1. [Q80TZ3-1]
CCDS51238.1. [Q80TZ3-3]
RefSeqiNP_001158055.1. NM_001164583.1. [Q80TZ3-3]
NP_001158056.1. NM_001164584.1. [Q80TZ3-1]
NP_001158057.1. NM_001164585.1. [Q80TZ3-2]
NP_940804.1. NM_198412.2. [Q80TZ3-2]
UniGeneiMm.76494.

Genome annotation databases

EnsembliENSMUST00000038207; ENSMUSP00000044251; ENSMUSG00000028528. [Q80TZ3-1]
ENSMUST00000094953; ENSMUSP00000092560; ENSMUSG00000028528. [Q80TZ3-2]
ENSMUST00000106929; ENSMUSP00000102542; ENSMUSG00000028528. [Q80TZ3-2]
ENSMUST00000106930; ENSMUSP00000102543; ENSMUSG00000028528. [Q80TZ3-2]
ENSMUST00000106933; ENSMUSP00000102546; ENSMUSG00000028528. [Q80TZ3-3]
GeneIDi72685.
KEGGimmu:72685.
UCSCiuc008tvq.2. mouse. [Q80TZ3-1]
uc008tvt.1. mouse. [Q80TZ3-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122293 mRNA. Translation: BAC65575.1 .
AK049935 mRNA. Translation: BAC33992.1 .
AK147570 mRNA. Translation: BAE28000.1 .
AK147637 mRNA. Translation: BAE28039.1 .
AK163657 mRNA. Translation: BAE37443.1 .
BX323551 Genomic DNA. Translation: CAM16148.1 .
BC060734 mRNA. Translation: AAH60734.1 . Different initiation.
BC064460 mRNA. Translation: AAH64460.1 .
CCDSi CCDS18395.1. [Q80TZ3-2 ]
CCDS51237.1. [Q80TZ3-1 ]
CCDS51238.1. [Q80TZ3-3 ]
RefSeqi NP_001158055.1. NM_001164583.1. [Q80TZ3-3 ]
NP_001158056.1. NM_001164584.1. [Q80TZ3-1 ]
NP_001158057.1. NM_001164585.1. [Q80TZ3-2 ]
NP_940804.1. NM_198412.2. [Q80TZ3-2 ]
UniGenei Mm.76494.

3D structure databases

ProteinModelPortali Q80TZ3.
SMRi Q80TZ3. Positions 81-427, 762-938.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q80TZ3. 4 interactions.
MINTi MINT-4114665.

PTM databases

PhosphoSitei Q80TZ3.

Proteomic databases

MaxQBi Q80TZ3.
PaxDbi Q80TZ3.
PRIDEi Q80TZ3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000038207 ; ENSMUSP00000044251 ; ENSMUSG00000028528 . [Q80TZ3-1 ]
ENSMUST00000094953 ; ENSMUSP00000092560 ; ENSMUSG00000028528 . [Q80TZ3-2 ]
ENSMUST00000106929 ; ENSMUSP00000102542 ; ENSMUSG00000028528 . [Q80TZ3-2 ]
ENSMUST00000106930 ; ENSMUSP00000102543 ; ENSMUSG00000028528 . [Q80TZ3-2 ]
ENSMUST00000106933 ; ENSMUSP00000102546 ; ENSMUSG00000028528 . [Q80TZ3-3 ]
GeneIDi 72685.
KEGGi mmu:72685.
UCSCi uc008tvq.2. mouse. [Q80TZ3-1 ]
uc008tvt.1. mouse. [Q80TZ3-3 ]

Organism-specific databases

CTDi 9829.
MGIi MGI:1919935. Dnajc6.
Rougei Search...

Phylogenomic databases

eggNOGi NOG269956.
GeneTreei ENSGT00760000119113.
HOGENOMi HOG000034235.
HOVERGENi HBG004322.
InParanoidi Q80TZ3.
KOi K09526.
OMAi LCWQDQK.
OrthoDBi EOG7WT40V.
PhylomeDBi Q80TZ3.
TreeFami TF105165.

Enzyme and pathway databases

Reactomei REACT_231722. Lysosome Vesicle Biogenesis.
REACT_232671. Golgi Associated Vesicle Biogenesis.

Miscellaneous databases

NextBioi 336731.
PROi Q80TZ3.
SOURCEi Search...

Gene expression databases

Bgeei Q80TZ3.
CleanExi MM_DNAJC6.
ExpressionAtlasi Q80TZ3. baseline and differential.
Genevestigatori Q80TZ3.

Family and domain databases

Gene3Di 1.10.287.110. 1 hit.
3.90.190.10. 1 hit.
InterProi IPR000008. C2_dom.
IPR001623. DnaJ_domain.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR014020. Tensin_C2-dom.
IPR029023. Tensin_lipid_phosphatase_dom.
[Graphical view ]
Pfami PF00226. DnaJ. 1 hit.
PF10409. PTEN_C2. 1 hit.
[Graphical view ]
SMARTi SM00271. DnaJ. 1 hit.
[Graphical view ]
SUPFAMi SSF46565. SSF46565. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF52799. SSF52799. 1 hit.
PROSITEi PS51182. C2_TENSIN. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51181. PPASE_TENSIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
    DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Brain, Hippocampus and Medulla oblongata.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
    Strain: C57BL/6.
    Tissue: Brain.
  5. "Endocytosis and clathrin-uncoating defects at synapses of auxilin knockout mice."
    Yim Y.I., Sun T., Wu L.G., Raimondi A., De Camilli P., Eisenberg E., Greene L.E.
    Proc. Natl. Acad. Sci. U.S.A. 107:4412-4417(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.

Entry informationi

Entry nameiAUXI_MOUSE
AccessioniPrimary (citable) accession number: Q80TZ3
Secondary accession number(s): B1B0B9, Q6P2K9, Q8C7L9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: June 27, 2006
Last modified: November 26, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3