Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Putative tyrosine-protein phosphatase auxilin

Gene

Dnajc6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Recruits HSPA8/HSC70 to clathrin-coated vesicles and promotes uncoating of clathrin-coated vesicles. Plays a role in clathrin-mediated endocytosis in neurons.1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei189Phosphocysteine intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • clathrin coat disassembly Source: MGI
  • regulation of clathrin-dependent endocytosis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Chaperone, Hydrolase, Protein phosphatase

Enzyme and pathway databases

ReactomeiR-MMU-432720. Lysosome Vesicle Biogenesis.
R-MMU-432722. Golgi Associated Vesicle Biogenesis.
R-MMU-8856828. Clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative tyrosine-protein phosphatase auxilin (EC:3.1.3.48)
Alternative name(s):
DnaJ homolog subfamily C member 6
Gene namesi
Name:Dnajc6
Synonyms:Kiaa0473
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1919935. Dnajc6.

Subcellular locationi

GO - Cellular componenti

  • postsynaptic density Source: MGI
  • synapse Source: MGI
  • vesicle Source: GO_Central
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Mice have a high rate of early postnatal mortality, although surviving pups have a normal life span despite decreased body weight. Knockout animals have impaired synaptic vesicle recycling, with an increased number of clathrin-coated vesicles, and impaired clathrin-mediated endocytosis of synaptic vesicles in neuronal culture. There is an up-regulation of Gak, but this does not fully compensate for the lack of the protein. The brains from mutant mice do not display alterations in substantia nigra morphology or dopamine transporter abundance or distribution, in agreement with the lack of gait or movement abnormalities in the mutant animals.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002445171 – 938Putative tyrosine-protein phosphatase auxilinAdd BLAST938

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei137PhosphoserineBy similarity1
Modified residuei478PhosphoserineCombined sources1
Modified residuei481PhosphoserineCombined sources1
Modified residuei595PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ80TZ3.
PeptideAtlasiQ80TZ3.
PRIDEiQ80TZ3.

PTM databases

iPTMnetiQ80TZ3.
PhosphoSitePlusiQ80TZ3.
SwissPalmiQ80TZ3.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028528.
CleanExiMM_DNAJC6.
ExpressionAtlasiQ80TZ3. baseline and differential.
GenevisibleiQ80TZ3. MM.

Interactioni

Subunit structurei

Interacts with HSPA8/HSC70. Interacts with CLTC. Interacts with AP2A2 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ80TZ3. 4 interactors.
MINTiMINT-4114665.
STRINGi10090.ENSMUSP00000102546.

Structurei

3D structure databases

ProteinModelPortaliQ80TZ3.
SMRiQ80TZ3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati61 – 6414
Repeati65 – 6824
Repeati69 – 7234
Domaini80 – 247Phosphatase tensin-typePROSITE-ProRule annotationAdd BLAST168
Domaini253 – 391C2 tensin-typePROSITE-ProRule annotationAdd BLAST139
Domaini874 – 938JPROSITE-ProRule annotationAdd BLAST65

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni61 – 723 X 4 AA approximate tandem repeatsAdd BLAST12

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi434 – 442SH3-bindingSequence analysis9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi491 – 785Pro-richAdd BLAST295

Sequence similaritiesi

Contains 1 C2 tensin-type domain.PROSITE-ProRule annotation
Contains 1 J domain.PROSITE-ProRule annotation
Contains 1 phosphatase tensin-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH3-binding

Phylogenomic databases

eggNOGiKOG0431. Eukaryota.
KOG2283. Eukaryota.
ENOG41129H2. LUCA.
GeneTreeiENSGT00760000119113.
HOGENOMiHOG000034235.
HOVERGENiHBG004322.
InParanoidiQ80TZ3.
KOiK09526.
OMAiSPQNKPN.
OrthoDBiEOG091G06HJ.
PhylomeDBiQ80TZ3.
TreeFamiTF105165.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR000008. C2_dom.
IPR001623. DnaJ_domain.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR014020. Tensin_C2-dom.
IPR029023. Tensin_lipid_phosphatase_dom.
[Graphical view]
PfamiPF10409. PTEN_C2. 1 hit.
[Graphical view]
SMARTiSM00271. DnaJ. 1 hit.
SM01326. PTEN_C2. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF52799. SSF52799. 1 hit.
PROSITEiPS51182. C2_TENSIN. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51181. PPASE_TENSIN. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80TZ3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTNPKSGVAE SAGLACSRAA AGENRMKDSE NKGASSPDME PSYGGGLFDM
60 70 80 90 100
VKGGAGRLFS NLKDNLKDTL KDTSSRVIQS VSSYTKGDLD FTYVTSRIIV
110 120 130 140 150
MSFPVDSVDI GFRNQVDDIR SFLDSRHLDH YTVYNLSPKS YRTAKFHSRV
160 170 180 190 200
SECSWPIRQA PSLHNLFAVC RNMYNWLLQN PKNVCVVHCL DGRAASSILV
210 220 230 240 250
GAMFIFCNLY STPGPAVRLL YAKRPGIGLS PSHRRYLGYM CDLLADKPYR
260 270 280 290 300
PHFKPLTIKA ITVSPVPFFN KQRNGCRPYC DVLIGETKIY STCTDFERMK
310 320 330 340 350
EYRVQDGKIF IPLNITVQGD VIVSMYHLRS TIGSRLQAKV TNTQIFQLQF
360 370 380 390 400
HSGFIPLDTT VLKFTKPELD ACDVPEKYPQ LFQVTLDIEV QPQDKVIDLT
410 420 430 440 450
PPWEHYCTKD VNPSILFSSQ QEHQDTLALG GQAPADLPPD HPRNLGQGGF
460 470 480 490 500
FASLCWQDQK SEKSRCEEDH AALVNQESEQ SDDELLTLSS PHGNAEGDKP
510 520 530 540 550
HGAKKPGKKQ QEPAAPPPPE EVDLLGLEGS DVSTNFSSLA APPSNSELLS
560 570 580 590 600
DLFGGVGATG PAQAGQAGVE DVFHPSGPVS AQSTPRRTAT SASASPTLRV
610 620 630 640 650
GEGATFDPFG APAKPPGQDL LGSFLNTSSA SSDPFLQPTR SPSPTVHASS
660 670 680 690 700
TPAVNIQPDI AGGWDWHTKP GGFGMGSKSA ATSPTGSSHG TPTHQSKPQT
710 720 730 740 750
LDPFADLGTL GSSSFASKPT TPTGLGGGFP PLSSPQKASP QPMGGGWQQP
760 770 780 790 800
AGYNWQQTQS KPQSSMPHSS PQNRPNYNVS FSAMPAGQSE RGKGSTNLEG
810 820 830 840 850
KQKAADFEDL LSSQGFNAHK DKKGPRTIAE MRKEEMAKEM DPEKLKILEW
860 870 880 890 900
IEGKERNIRA LLSTMHTVLW AGETKWKPVG MADLVTPEQV KKVYRRAVLV
910 920 930
VHPDKATGQP YEQYAKMIFM ELNDAWSEFE NQGQKPLY
Length:938
Mass (Da):102,299
Last modified:June 27, 2006 - v2
Checksum:i3DFB7D9275BEF3F6
GO
Isoform 2 (identifier: Q80TZ3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-38: Missing.

Show »
Length:900
Mass (Da):98,521
Checksum:i9D35C6511ECD7BE5
GO
Isoform 3 (identifier: Q80TZ3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: MTNPKSGVAESAGLACSRAAAGENRMKDSENK → MSLLGSYRKK...PHRASTTDSS

Show »
Length:968
Mass (Da):105,409
Checksum:i83C4E04C24405E01
GO

Sequence cautioni

The sequence AAH60734 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti442P → T in BAC65575 (PubMed:12693553).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0195821 – 38Missing in isoform 2. 2 PublicationsAdd BLAST38
Alternative sequenceiVSP_0195831 – 32MTNPK…DSENK → MSLLGSYRKKTSSDGYESLQ LVDSHGDSSARGAAAGTQRA TAGAVRSPARQPPHRASTTD SS in isoform 3. 1 PublicationAdd BLAST32

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122293 mRNA. Translation: BAC65575.1.
AK049935 mRNA. Translation: BAC33992.1.
AK147570 mRNA. Translation: BAE28000.1.
AK147637 mRNA. Translation: BAE28039.1.
AK163657 mRNA. Translation: BAE37443.1.
BX323551 Genomic DNA. Translation: CAM16148.1.
BC060734 mRNA. Translation: AAH60734.1. Different initiation.
BC064460 mRNA. Translation: AAH64460.1.
CCDSiCCDS18395.1. [Q80TZ3-2]
CCDS51237.1. [Q80TZ3-1]
CCDS51238.1. [Q80TZ3-3]
RefSeqiNP_001158055.1. NM_001164583.1. [Q80TZ3-3]
NP_001158056.1. NM_001164584.1. [Q80TZ3-1]
NP_001158057.1. NM_001164585.1. [Q80TZ3-2]
NP_940804.1. NM_198412.2. [Q80TZ3-2]
XP_017175882.1. XM_017320393.1. [Q80TZ3-2]
UniGeneiMm.76494.

Genome annotation databases

EnsembliENSMUST00000038207; ENSMUSP00000044251; ENSMUSG00000028528. [Q80TZ3-1]
ENSMUST00000094953; ENSMUSP00000092560; ENSMUSG00000028528. [Q80TZ3-2]
ENSMUST00000106929; ENSMUSP00000102542; ENSMUSG00000028528. [Q80TZ3-2]
ENSMUST00000106930; ENSMUSP00000102543; ENSMUSG00000028528. [Q80TZ3-2]
ENSMUST00000106933; ENSMUSP00000102546; ENSMUSG00000028528. [Q80TZ3-3]
GeneIDi72685.
KEGGimmu:72685.
UCSCiuc008tvq.2. mouse. [Q80TZ3-1]
uc008tvt.1. mouse. [Q80TZ3-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122293 mRNA. Translation: BAC65575.1.
AK049935 mRNA. Translation: BAC33992.1.
AK147570 mRNA. Translation: BAE28000.1.
AK147637 mRNA. Translation: BAE28039.1.
AK163657 mRNA. Translation: BAE37443.1.
BX323551 Genomic DNA. Translation: CAM16148.1.
BC060734 mRNA. Translation: AAH60734.1. Different initiation.
BC064460 mRNA. Translation: AAH64460.1.
CCDSiCCDS18395.1. [Q80TZ3-2]
CCDS51237.1. [Q80TZ3-1]
CCDS51238.1. [Q80TZ3-3]
RefSeqiNP_001158055.1. NM_001164583.1. [Q80TZ3-3]
NP_001158056.1. NM_001164584.1. [Q80TZ3-1]
NP_001158057.1. NM_001164585.1. [Q80TZ3-2]
NP_940804.1. NM_198412.2. [Q80TZ3-2]
XP_017175882.1. XM_017320393.1. [Q80TZ3-2]
UniGeneiMm.76494.

3D structure databases

ProteinModelPortaliQ80TZ3.
SMRiQ80TZ3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ80TZ3. 4 interactors.
MINTiMINT-4114665.
STRINGi10090.ENSMUSP00000102546.

PTM databases

iPTMnetiQ80TZ3.
PhosphoSitePlusiQ80TZ3.
SwissPalmiQ80TZ3.

Proteomic databases

PaxDbiQ80TZ3.
PeptideAtlasiQ80TZ3.
PRIDEiQ80TZ3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038207; ENSMUSP00000044251; ENSMUSG00000028528. [Q80TZ3-1]
ENSMUST00000094953; ENSMUSP00000092560; ENSMUSG00000028528. [Q80TZ3-2]
ENSMUST00000106929; ENSMUSP00000102542; ENSMUSG00000028528. [Q80TZ3-2]
ENSMUST00000106930; ENSMUSP00000102543; ENSMUSG00000028528. [Q80TZ3-2]
ENSMUST00000106933; ENSMUSP00000102546; ENSMUSG00000028528. [Q80TZ3-3]
GeneIDi72685.
KEGGimmu:72685.
UCSCiuc008tvq.2. mouse. [Q80TZ3-1]
uc008tvt.1. mouse. [Q80TZ3-3]

Organism-specific databases

CTDi9829.
MGIiMGI:1919935. Dnajc6.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0431. Eukaryota.
KOG2283. Eukaryota.
ENOG41129H2. LUCA.
GeneTreeiENSGT00760000119113.
HOGENOMiHOG000034235.
HOVERGENiHBG004322.
InParanoidiQ80TZ3.
KOiK09526.
OMAiSPQNKPN.
OrthoDBiEOG091G06HJ.
PhylomeDBiQ80TZ3.
TreeFamiTF105165.

Enzyme and pathway databases

ReactomeiR-MMU-432720. Lysosome Vesicle Biogenesis.
R-MMU-432722. Golgi Associated Vesicle Biogenesis.
R-MMU-8856828. Clathrin-mediated endocytosis.

Miscellaneous databases

PROiQ80TZ3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028528.
CleanExiMM_DNAJC6.
ExpressionAtlasiQ80TZ3. baseline and differential.
GenevisibleiQ80TZ3. MM.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR000008. C2_dom.
IPR001623. DnaJ_domain.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR014020. Tensin_C2-dom.
IPR029023. Tensin_lipid_phosphatase_dom.
[Graphical view]
PfamiPF10409. PTEN_C2. 1 hit.
[Graphical view]
SMARTiSM00271. DnaJ. 1 hit.
SM01326. PTEN_C2. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF52799. SSF52799. 1 hit.
PROSITEiPS51182. C2_TENSIN. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51181. PPASE_TENSIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAUXI_MOUSE
AccessioniPrimary (citable) accession number: Q80TZ3
Secondary accession number(s): B1B0B9, Q6P2K9, Q8C7L9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: June 27, 2006
Last modified: November 2, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.