Q80TZ3 (AUXI_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative tyrosine-protein phosphatase auxilin
EC=3.1.3.48

Alternative name(s):
DnaJ homolog subfamily C member 6

Gene names

Name:	Dnajc6
Synonyms:	Kiaa0473

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	938 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Recruits HSPA8/HSC70 to clathrin-coated vesicles and promotes uncoating of clathrin-coated vesicles By similarity.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Subunit structure	Interacts with HSPA8/HSC70. Interacts with CLTC. Interacts with AP2A2 By similarity.
Sequence similarities	Contains 1 C2 tensin-type domain. Contains 1 J domain. Contains 1 phosphatase tensin-type domain.
Sequence caution	The sequence AAH60734.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	Repeat SH3-binding
Molecular function	Chaperone Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC
Molecular_function	protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q80TZ3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q80TZ3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-38: Missing.

Isoform 3 (identifier: Q80TZ3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: MTNPKSGVAESAGLACSRAAAGENRMKDSENK → MSLLGSYRKK...PHRASTTDSS

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 938	938	Putative tyrosine-protein phosphatase auxilin		PRO_0000244517
Regions
	Repeat	61 – 64	4	1
	Repeat	65 – 68	4	2
	Repeat	69 – 72	4	3
	Domain	80 – 247	168	Phosphatase tensin-type
	Domain	253 – 391	139	C2 tensin-type
	Domain	874 – 938	65	J
	Region	61 – 72	12	3 X 4 AA approximate tandem repeats
	Motif	434 – 442	9	SH3-binding Potential
	Compositional bias	491 – 785	295	Pro-rich
Sites
	Active site	189	1	Phosphocysteine intermediate Potential
Amino acid modifications
	Modified residue	595	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 38	38	Missing in isoform 2.		VSP_019582
	Alternative sequence	1 – 32	32	MTNPK…DSENK → MSLLGSYRKKTSSDGYESLQ LVDSHGDSSARGAAAGTQRA TAGAVRSPARQPPHRASTTD SS in isoform 3.		VSP_019583
Experimental info
	Sequence conflict	442	1	P → T in BAC65575. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 27, 2006. Version 2. Checksum: 3DFB7D9275BEF3F6	FASTA	938	102,299
	10 20 30 40 50 60 MTNPKSGVAE SAGLACSRAA AGENRMKDSE NKGASSPDME PSYGGGLFDM VKGGAGRLFS 70 80 90 100 110 120 NLKDNLKDTL KDTSSRVIQS VSSYTKGDLD FTYVTSRIIV MSFPVDSVDI GFRNQVDDIR 130 140 150 160 170 180 SFLDSRHLDH YTVYNLSPKS YRTAKFHSRV SECSWPIRQA PSLHNLFAVC RNMYNWLLQN 190 200 210 220 230 240 PKNVCVVHCL DGRAASSILV GAMFIFCNLY STPGPAVRLL YAKRPGIGLS PSHRRYLGYM 250 260 270 280 290 300 CDLLADKPYR PHFKPLTIKA ITVSPVPFFN KQRNGCRPYC DVLIGETKIY STCTDFERMK 310 320 330 340 350 360 EYRVQDGKIF IPLNITVQGD VIVSMYHLRS TIGSRLQAKV TNTQIFQLQF HSGFIPLDTT 370 380 390 400 410 420 VLKFTKPELD ACDVPEKYPQ LFQVTLDIEV QPQDKVIDLT PPWEHYCTKD VNPSILFSSQ 430 440 450 460 470 480 QEHQDTLALG GQAPADLPPD HPRNLGQGGF FASLCWQDQK SEKSRCEEDH AALVNQESEQ 490 500 510 520 530 540 SDDELLTLSS PHGNAEGDKP HGAKKPGKKQ QEPAAPPPPE EVDLLGLEGS DVSTNFSSLA 550 560 570 580 590 600 APPSNSELLS DLFGGVGATG PAQAGQAGVE DVFHPSGPVS AQSTPRRTAT SASASPTLRV 610 620 630 640 650 660 GEGATFDPFG APAKPPGQDL LGSFLNTSSA SSDPFLQPTR SPSPTVHASS TPAVNIQPDI 670 680 690 700 710 720 AGGWDWHTKP GGFGMGSKSA ATSPTGSSHG TPTHQSKPQT LDPFADLGTL GSSSFASKPT 730 740 750 760 770 780 TPTGLGGGFP PLSSPQKASP QPMGGGWQQP AGYNWQQTQS KPQSSMPHSS PQNRPNYNVS 790 800 810 820 830 840 FSAMPAGQSE RGKGSTNLEG KQKAADFEDL LSSQGFNAHK DKKGPRTIAE MRKEEMAKEM 850 860 870 880 890 900 DPEKLKILEW IEGKERNIRA LLSTMHTVLW AGETKWKPVG MADLVTPEQV KKVYRRAVLV 910 920 930 VHPDKATGQP YEQYAKMIFM ELNDAWSEFE NQGQKPLY
	Isoform 2 [UniParc]. Checksum: 9D35C6511ECD7BE5	FASTA	900	98,521
	10 20 30 40 50 60 MEPSYGGGLF DMVKGGAGRL FSNLKDNLKD TLKDTSSRVI QSVSSYTKGD LDFTYVTSRI 70 80 90 100 110 120 IVMSFPVDSV DIGFRNQVDD IRSFLDSRHL DHYTVYNLSP KSYRTAKFHS RVSECSWPIR 130 140 150 160 170 180 QAPSLHNLFA VCRNMYNWLL QNPKNVCVVH CLDGRAASSI LVGAMFIFCN LYSTPGPAVR 190 200 210 220 230 240 LLYAKRPGIG LSPSHRRYLG YMCDLLADKP YRPHFKPLTI KAITVSPVPF FNKQRNGCRP 250 260 270 280 290 300 YCDVLIGETK IYSTCTDFER MKEYRVQDGK IFIPLNITVQ GDVIVSMYHL RSTIGSRLQA 310 320 330 340 350 360 KVTNTQIFQL QFHSGFIPLD TTVLKFTKPE LDACDVPEKY PQLFQVTLDI EVQPQDKVID 370 380 390 400 410 420 LTPPWEHYCT KDVNPSILFS SQQEHQDTLA LGGQAPADLP PDHPRNLGQG GFFASLCWQD 430 440 450 460 470 480 QKSEKSRCEE DHAALVNQES EQSDDELLTL SSPHGNAEGD KPHGAKKPGK KQQEPAAPPP 490 500 510 520 530 540 PEEVDLLGLE GSDVSTNFSS LAAPPSNSEL LSDLFGGVGA TGPAQAGQAG VEDVFHPSGP 550 560 570 580 590 600 VSAQSTPRRT ATSASASPTL RVGEGATFDP FGAPAKPPGQ DLLGSFLNTS SASSDPFLQP 610 620 630 640 650 660 TRSPSPTVHA SSTPAVNIQP DIAGGWDWHT KPGGFGMGSK SAATSPTGSS HGTPTHQSKP 670 680 690 700 710 720 QTLDPFADLG TLGSSSFASK PTTPTGLGGG FPPLSSPQKA SPQPMGGGWQ QPAGYNWQQT 730 740 750 760 770 780 QSKPQSSMPH SSPQNRPNYN VSFSAMPAGQ SERGKGSTNL EGKQKAADFE DLLSSQGFNA 790 800 810 820 830 840 HKDKKGPRTI AEMRKEEMAK EMDPEKLKIL EWIEGKERNI RALLSTMHTV LWAGETKWKP 850 860 870 880 890 900 VGMADLVTPE QVKKVYRRAV LVVHPDKATG QPYEQYAKMI FMELNDAWSE FENQGQKPLY
	Isoform 3 [UniParc]. Checksum: 83C4E04C24405E01	FASTA	968	105,409
	10 20 30 40 50 60 MSLLGSYRKK TSSDGYESLQ LVDSHGDSSA RGAAAGTQRA TAGAVRSPAR QPPHRASTTD 70 80 90 100 110 120 SSGASSPDME PSYGGGLFDM VKGGAGRLFS NLKDNLKDTL KDTSSRVIQS VSSYTKGDLD 130 140 150 160 170 180 FTYVTSRIIV MSFPVDSVDI GFRNQVDDIR SFLDSRHLDH YTVYNLSPKS YRTAKFHSRV 190 200 210 220 230 240 SECSWPIRQA PSLHNLFAVC RNMYNWLLQN PKNVCVVHCL DGRAASSILV GAMFIFCNLY 250 260 270 280 290 300 STPGPAVRLL YAKRPGIGLS PSHRRYLGYM CDLLADKPYR PHFKPLTIKA ITVSPVPFFN 310 320 330 340 350 360 KQRNGCRPYC DVLIGETKIY STCTDFERMK EYRVQDGKIF IPLNITVQGD VIVSMYHLRS 370 380 390 400 410 420 TIGSRLQAKV TNTQIFQLQF HSGFIPLDTT VLKFTKPELD ACDVPEKYPQ LFQVTLDIEV 430 440 450 460 470 480 QPQDKVIDLT PPWEHYCTKD VNPSILFSSQ QEHQDTLALG GQAPADLPPD HPRNLGQGGF 490 500 510 520 530 540 FASLCWQDQK SEKSRCEEDH AALVNQESEQ SDDELLTLSS PHGNAEGDKP HGAKKPGKKQ 550 560 570 580 590 600 QEPAAPPPPE EVDLLGLEGS DVSTNFSSLA APPSNSELLS DLFGGVGATG PAQAGQAGVE 610 620 630 640 650 660 DVFHPSGPVS AQSTPRRTAT SASASPTLRV GEGATFDPFG APAKPPGQDL LGSFLNTSSA 670 680 690 700 710 720 SSDPFLQPTR SPSPTVHASS TPAVNIQPDI AGGWDWHTKP GGFGMGSKSA ATSPTGSSHG 730 740 750 760 770 780 TPTHQSKPQT LDPFADLGTL GSSSFASKPT TPTGLGGGFP PLSSPQKASP QPMGGGWQQP 790 800 810 820 830 840 AGYNWQQTQS KPQSSMPHSS PQNRPNYNVS FSAMPAGQSE RGKGSTNLEG KQKAADFEDL 850 860 870 880 890 900 LSSQGFNAHK DKKGPRTIAE MRKEEMAKEM DPEKLKILEW IEGKERNIRA LLSTMHTVLW 910 920 930 940 950 960 AGETKWKPVG MADLVTPEQV KKVYRRAVLV VHPDKATGQP YEQYAKMIFM ELNDAWSEFE NQGQKPLY

References

[1]	"Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H. DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J. Tissue: Brain, Hippocampus and Medulla oblongata.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK122293 mRNA. Translation: BAC65575.1. AK049935 mRNA. Translation: BAC33992.1. AK147570 mRNA. Translation: BAE28000.1. AK147637 mRNA. Translation: BAE28039.1. AK163657 mRNA. Translation: BAE37443.1. BX323551 Genomic DNA. Translation: CAM16148.1. BC060734 mRNA. Translation: AAH60734.1. Different initiation. BC064460 mRNA. Translation: AAH64460.1.
IPI	IPI00330269. IPI00650037. IPI00762713.
RefSeq	NP_001158055.1. NM_001164583.1. NP_001158056.1. NM_001164584.1. NP_001158057.1. NM_001164585.1. NP_940804.1. NM_198412.2.
UniGene	Mm.76494.
3D structure databases
ProteinModelPortal	Q80TZ3.
SMR	Q80TZ3. Positions 81-427, 762-938.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q80TZ3. 3 interactions.
PTM databases
PhosphoSite	Q80TZ3.
Proteomic databases
PaxDb	Q80TZ3.
PRIDE	Q80TZ3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000038207; ENSMUSP00000044251; ENSMUSG00000028528. ENSMUST00000094953; ENSMUSP00000092560; ENSMUSG00000028528. ENSMUST00000106929; ENSMUSP00000102542; ENSMUSG00000028528. ENSMUST00000106930; ENSMUSP00000102543; ENSMUSG00000028528. ENSMUST00000106933; ENSMUSP00000102546; ENSMUSG00000028528.
GeneID	72685.
KEGG	mmu:72685.
UCSC	uc008tvq.2. mouse. uc008tvt.1. mouse.
Organism-specific databases
CTD	9829.
MGI	MGI:1919935. Dnajc6.
Rouge	Search...
Phylogenomic databases
eggNOG	NOG269956.
GeneTree	ENSGT00620000087779.
HOGENOM	HOG000034235.
HOVERGEN	HBG004322.
InParanoid	B1B0B9.
KO	K09526.
OMA	LCWQDQK.
Gene expression databases
ArrayExpress	Q80TZ3.
Bgee	Q80TZ3.
CleanEx	MM_DNAJC6.
Genevestigator	Q80TZ3.
GermOnline	ENSMUSG00000028528. Mus musculus.
Family and domain databases
Gene3D	1.10.287.110. 1 hit.
InterPro	IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR001623. DnaJ_domain. IPR014019. Phosphatase_tensin-typ. IPR014020. Tensin_phosphatase_C2-dom. [Graphical view]
Pfam	PF00226. DnaJ. 1 hit. PF10409. PTEN_C2. 1 hit. [Graphical view]
SMART	SM00271. DnaJ. 1 hit. [Graphical view]
SUPFAM	SSF49562. C2_CaLB. 1 hit. SSF46565. DnaJ_N. 1 hit.
PROSITE	PS51182. C2_TENSIN. 1 hit. PS00636. DNAJ_1. False negative. PS50076. DNAJ_2. 1 hit. PS51181. PPASE_TENSIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	336731.
SOURCE	Search...

Entry information

Entry name

AUXI_MOUSE

Accession

Primary (citable) accession number: Q80TZ3
Secondary accession number(s): B1B0B9, Q6P2K9, Q8C7L9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	June 27, 2006
Last modified:	May 1, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program