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Q80TZ3

- AUXI_MOUSE

UniProt

Q80TZ3 - AUXI_MOUSE

Protein

Putative tyrosine-protein phosphatase auxilin

Gene

Dnajc6

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 2 (27 Jun 2006)
      Previous versions | rss
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    Functioni

    Recruits HSPA8/HSC70 to clathrin-coated vesicles and promotes uncoating of clathrin-coated vesicles. Plays a role in clathrin-mediated endocytosis in neurons.1 Publication

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei189 – 1891Phosphocysteine intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. protein tyrosine phosphatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. clathrin coat disassembly Source: MGI
    2. regulation of clathrin-mediated endocytosis Source: MGI

    Keywords - Molecular functioni

    Chaperone, Hydrolase, Protein phosphatase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative tyrosine-protein phosphatase auxilin (EC:3.1.3.48)
    Alternative name(s):
    DnaJ homolog subfamily C member 6
    Gene namesi
    Name:Dnajc6
    Synonyms:Kiaa0473
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:1919935. Dnajc6.

    Subcellular locationi

    GO - Cellular componenti

    1. synapse Source: MGI

    Pathology & Biotechi

    Disruption phenotypei

    Mice have a high rate of early postnatal mortality, although surviving pups have a normal life span despite decreased body weight. Knockout animals have impaired synaptic vesicle recycling, with an increased number of clathrin-coated vesicles, and impaired clathrin-mediated endocytosis of synaptic vesicles in neuronal culture. There is an up-regulation of Gak, but this does not fully compensate for the lack of the protein. The brains from mutant mice do not display alterations in substantia nigra morphology or dopamine transporter abundance or distribution, in agreement with the lack of gait or movement abnormalities in the mutant animals.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 938938Putative tyrosine-protein phosphatase auxilinPRO_0000244517Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei595 – 5951PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ80TZ3.
    PaxDbiQ80TZ3.
    PRIDEiQ80TZ3.

    PTM databases

    PhosphoSiteiQ80TZ3.

    Expressioni

    Gene expression databases

    ArrayExpressiQ80TZ3.
    BgeeiQ80TZ3.
    CleanExiMM_DNAJC6.
    GenevestigatoriQ80TZ3.

    Interactioni

    Subunit structurei

    Interacts with HSPA8/HSC70. Interacts with CLTC. Interacts with AP2A2 By similarity.By similarity

    Protein-protein interaction databases

    IntActiQ80TZ3. 4 interactions.
    MINTiMINT-4114665.

    Structurei

    3D structure databases

    ProteinModelPortaliQ80TZ3.
    SMRiQ80TZ3. Positions 81-427, 762-938.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati61 – 6441
    Repeati65 – 6842
    Repeati69 – 7243
    Domaini80 – 247168Phosphatase tensin-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini253 – 391139C2 tensin-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini874 – 93865JPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni61 – 72123 X 4 AA approximate tandem repeatsAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi434 – 4429SH3-bindingSequence Analysis

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi491 – 785295Pro-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 C2 tensin-type domain.PROSITE-ProRule annotation
    Contains 1 J domain.PROSITE-ProRule annotation
    Contains 1 phosphatase tensin-type domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, SH3-binding

    Phylogenomic databases

    eggNOGiNOG269956.
    GeneTreeiENSGT00620000087779.
    HOGENOMiHOG000034235.
    HOVERGENiHBG004322.
    InParanoidiB1B0B9.
    KOiK09526.
    OMAiLCWQDQK.
    OrthoDBiEOG7WT40V.
    PhylomeDBiQ80TZ3.
    TreeFamiTF105165.

    Family and domain databases

    Gene3Di1.10.287.110. 1 hit.
    3.90.190.10. 1 hit.
    InterProiIPR000008. C2_dom.
    IPR001623. DnaJ_domain.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR014020. Tensin_C2-dom.
    IPR029023. Tensin_lipid_phosphatase_dom.
    [Graphical view]
    PfamiPF00226. DnaJ. 1 hit.
    PF10409. PTEN_C2. 1 hit.
    [Graphical view]
    SMARTiSM00271. DnaJ. 1 hit.
    [Graphical view]
    SUPFAMiSSF46565. SSF46565. 1 hit.
    SSF49562. SSF49562. 1 hit.
    SSF52799. SSF52799. 1 hit.
    PROSITEiPS51182. C2_TENSIN. 1 hit.
    PS50076. DNAJ_2. 1 hit.
    PS51181. PPASE_TENSIN. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q80TZ3-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTNPKSGVAE SAGLACSRAA AGENRMKDSE NKGASSPDME PSYGGGLFDM    50
    VKGGAGRLFS NLKDNLKDTL KDTSSRVIQS VSSYTKGDLD FTYVTSRIIV 100
    MSFPVDSVDI GFRNQVDDIR SFLDSRHLDH YTVYNLSPKS YRTAKFHSRV 150
    SECSWPIRQA PSLHNLFAVC RNMYNWLLQN PKNVCVVHCL DGRAASSILV 200
    GAMFIFCNLY STPGPAVRLL YAKRPGIGLS PSHRRYLGYM CDLLADKPYR 250
    PHFKPLTIKA ITVSPVPFFN KQRNGCRPYC DVLIGETKIY STCTDFERMK 300
    EYRVQDGKIF IPLNITVQGD VIVSMYHLRS TIGSRLQAKV TNTQIFQLQF 350
    HSGFIPLDTT VLKFTKPELD ACDVPEKYPQ LFQVTLDIEV QPQDKVIDLT 400
    PPWEHYCTKD VNPSILFSSQ QEHQDTLALG GQAPADLPPD HPRNLGQGGF 450
    FASLCWQDQK SEKSRCEEDH AALVNQESEQ SDDELLTLSS PHGNAEGDKP 500
    HGAKKPGKKQ QEPAAPPPPE EVDLLGLEGS DVSTNFSSLA APPSNSELLS 550
    DLFGGVGATG PAQAGQAGVE DVFHPSGPVS AQSTPRRTAT SASASPTLRV 600
    GEGATFDPFG APAKPPGQDL LGSFLNTSSA SSDPFLQPTR SPSPTVHASS 650
    TPAVNIQPDI AGGWDWHTKP GGFGMGSKSA ATSPTGSSHG TPTHQSKPQT 700
    LDPFADLGTL GSSSFASKPT TPTGLGGGFP PLSSPQKASP QPMGGGWQQP 750
    AGYNWQQTQS KPQSSMPHSS PQNRPNYNVS FSAMPAGQSE RGKGSTNLEG 800
    KQKAADFEDL LSSQGFNAHK DKKGPRTIAE MRKEEMAKEM DPEKLKILEW 850
    IEGKERNIRA LLSTMHTVLW AGETKWKPVG MADLVTPEQV KKVYRRAVLV 900
    VHPDKATGQP YEQYAKMIFM ELNDAWSEFE NQGQKPLY 938
    Length:938
    Mass (Da):102,299
    Last modified:June 27, 2006 - v2
    Checksum:i3DFB7D9275BEF3F6
    GO
    Isoform 2 (identifier: Q80TZ3-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-38: Missing.

    Show »
    Length:900
    Mass (Da):98,521
    Checksum:i9D35C6511ECD7BE5
    GO
    Isoform 3 (identifier: Q80TZ3-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-32: MTNPKSGVAESAGLACSRAAAGENRMKDSENK → MSLLGSYRKK...PHRASTTDSS

    Show »
    Length:968
    Mass (Da):105,409
    Checksum:i83C4E04C24405E01
    GO

    Sequence cautioni

    The sequence AAH60734.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti442 – 4421P → T in BAC65575. (PubMed:12693553)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 3838Missing in isoform 2. 2 PublicationsVSP_019582Add
    BLAST
    Alternative sequencei1 – 3232MTNPK…DSENK → MSLLGSYRKKTSSDGYESLQ LVDSHGDSSARGAAAGTQRA TAGAVRSPARQPPHRASTTD SS in isoform 3. 1 PublicationVSP_019583Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK122293 mRNA. Translation: BAC65575.1.
    AK049935 mRNA. Translation: BAC33992.1.
    AK147570 mRNA. Translation: BAE28000.1.
    AK147637 mRNA. Translation: BAE28039.1.
    AK163657 mRNA. Translation: BAE37443.1.
    BX323551 Genomic DNA. Translation: CAM16148.1.
    BC060734 mRNA. Translation: AAH60734.1. Different initiation.
    BC064460 mRNA. Translation: AAH64460.1.
    CCDSiCCDS18395.1. [Q80TZ3-2]
    CCDS51237.1. [Q80TZ3-1]
    CCDS51238.1. [Q80TZ3-3]
    RefSeqiNP_001158055.1. NM_001164583.1. [Q80TZ3-3]
    NP_001158056.1. NM_001164584.1. [Q80TZ3-1]
    NP_001158057.1. NM_001164585.1. [Q80TZ3-2]
    NP_940804.1. NM_198412.2. [Q80TZ3-2]
    UniGeneiMm.76494.

    Genome annotation databases

    EnsembliENSMUST00000038207; ENSMUSP00000044251; ENSMUSG00000028528. [Q80TZ3-1]
    ENSMUST00000094953; ENSMUSP00000092560; ENSMUSG00000028528. [Q80TZ3-2]
    ENSMUST00000106929; ENSMUSP00000102542; ENSMUSG00000028528. [Q80TZ3-2]
    ENSMUST00000106930; ENSMUSP00000102543; ENSMUSG00000028528. [Q80TZ3-2]
    ENSMUST00000106933; ENSMUSP00000102546; ENSMUSG00000028528. [Q80TZ3-3]
    GeneIDi72685.
    KEGGimmu:72685.
    UCSCiuc008tvq.2. mouse. [Q80TZ3-1]
    uc008tvt.1. mouse. [Q80TZ3-3]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK122293 mRNA. Translation: BAC65575.1 .
    AK049935 mRNA. Translation: BAC33992.1 .
    AK147570 mRNA. Translation: BAE28000.1 .
    AK147637 mRNA. Translation: BAE28039.1 .
    AK163657 mRNA. Translation: BAE37443.1 .
    BX323551 Genomic DNA. Translation: CAM16148.1 .
    BC060734 mRNA. Translation: AAH60734.1 . Different initiation.
    BC064460 mRNA. Translation: AAH64460.1 .
    CCDSi CCDS18395.1. [Q80TZ3-2 ]
    CCDS51237.1. [Q80TZ3-1 ]
    CCDS51238.1. [Q80TZ3-3 ]
    RefSeqi NP_001158055.1. NM_001164583.1. [Q80TZ3-3 ]
    NP_001158056.1. NM_001164584.1. [Q80TZ3-1 ]
    NP_001158057.1. NM_001164585.1. [Q80TZ3-2 ]
    NP_940804.1. NM_198412.2. [Q80TZ3-2 ]
    UniGenei Mm.76494.

    3D structure databases

    ProteinModelPortali Q80TZ3.
    SMRi Q80TZ3. Positions 81-427, 762-938.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q80TZ3. 4 interactions.
    MINTi MINT-4114665.

    PTM databases

    PhosphoSitei Q80TZ3.

    Proteomic databases

    MaxQBi Q80TZ3.
    PaxDbi Q80TZ3.
    PRIDEi Q80TZ3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000038207 ; ENSMUSP00000044251 ; ENSMUSG00000028528 . [Q80TZ3-1 ]
    ENSMUST00000094953 ; ENSMUSP00000092560 ; ENSMUSG00000028528 . [Q80TZ3-2 ]
    ENSMUST00000106929 ; ENSMUSP00000102542 ; ENSMUSG00000028528 . [Q80TZ3-2 ]
    ENSMUST00000106930 ; ENSMUSP00000102543 ; ENSMUSG00000028528 . [Q80TZ3-2 ]
    ENSMUST00000106933 ; ENSMUSP00000102546 ; ENSMUSG00000028528 . [Q80TZ3-3 ]
    GeneIDi 72685.
    KEGGi mmu:72685.
    UCSCi uc008tvq.2. mouse. [Q80TZ3-1 ]
    uc008tvt.1. mouse. [Q80TZ3-3 ]

    Organism-specific databases

    CTDi 9829.
    MGIi MGI:1919935. Dnajc6.
    Rougei Search...

    Phylogenomic databases

    eggNOGi NOG269956.
    GeneTreei ENSGT00620000087779.
    HOGENOMi HOG000034235.
    HOVERGENi HBG004322.
    InParanoidi B1B0B9.
    KOi K09526.
    OMAi LCWQDQK.
    OrthoDBi EOG7WT40V.
    PhylomeDBi Q80TZ3.
    TreeFami TF105165.

    Miscellaneous databases

    NextBioi 336731.
    PROi Q80TZ3.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q80TZ3.
    Bgeei Q80TZ3.
    CleanExi MM_DNAJC6.
    Genevestigatori Q80TZ3.

    Family and domain databases

    Gene3Di 1.10.287.110. 1 hit.
    3.90.190.10. 1 hit.
    InterProi IPR000008. C2_dom.
    IPR001623. DnaJ_domain.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR014020. Tensin_C2-dom.
    IPR029023. Tensin_lipid_phosphatase_dom.
    [Graphical view ]
    Pfami PF00226. DnaJ. 1 hit.
    PF10409. PTEN_C2. 1 hit.
    [Graphical view ]
    SMARTi SM00271. DnaJ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46565. SSF46565. 1 hit.
    SSF49562. SSF49562. 1 hit.
    SSF52799. SSF52799. 1 hit.
    PROSITEi PS51182. C2_TENSIN. 1 hit.
    PS50076. DNAJ_2. 1 hit.
    PS51181. PPASE_TENSIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
      DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Brain, Hippocampus and Medulla oblongata.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
      Strain: C57BL/6.
      Tissue: Brain.
    5. "Endocytosis and clathrin-uncoating defects at synapses of auxilin knockout mice."
      Yim Y.I., Sun T., Wu L.G., Raimondi A., De Camilli P., Eisenberg E., Greene L.E.
      Proc. Natl. Acad. Sci. U.S.A. 107:4412-4417(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, FUNCTION.

    Entry informationi

    Entry nameiAUXI_MOUSE
    AccessioniPrimary (citable) accession number: Q80TZ3
    Secondary accession number(s): B1B0B9, Q6P2K9, Q8C7L9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 27, 2006
    Last sequence update: June 27, 2006
    Last modified: October 1, 2014
    This is version 90 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3