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Protein

CLIP-associating protein 1

Gene

Clasp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
CLIP-associating protein 1
Alternative name(s):
Cytoplasmic linker-associated protein 1
Gene namesi
Name:Clasp1
Synonyms:Kiaa0622
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1923957. Clasp1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Golgi apparatus, Kinetochore, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15351535CLIP-associating protein 1PRO_0000272274Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei246 – 2461PhosphoserineCombined sources
Modified residuei545 – 5451PhosphoserineCombined sources
Modified residuei548 – 5481PhosphoserineCombined sources
Modified residuei558 – 5581PhosphoserineBy similarity
Modified residuei559 – 5591PhosphoserineBy similarity
Modified residuei568 – 5681PhosphoserineBy similarity
Modified residuei600 – 6001PhosphoserineCombined sources
Modified residuei636 – 6361PhosphoserineCombined sources
Modified residuei646 – 6461PhosphoserineBy similarity
Modified residuei647 – 6471PhosphoserineBy similarity
Modified residuei649 – 6491PhosphoserineBy similarity
Modified residuei656 – 6561PhosphothreonineBy similarity
Modified residuei684 – 6841PhosphoserineBy similarity
Modified residuei688 – 6881PhosphoserineCombined sources
Modified residuei695 – 6951PhosphoserineBy similarity
Modified residuei702 – 7021PhosphoserineBy similarity
Modified residuei708 – 7081PhosphothreonineBy similarity
Modified residuei711 – 7111PhosphoserineBy similarity
Modified residuei784 – 7841PhosphoserineBy similarity
Modified residuei794 – 7941PhosphoserineBy similarity
Modified residuei820 – 8201PhosphoserineCombined sources
Modified residuei1088 – 10881PhosphoserineCombined sources
Modified residuei1092 – 10921PhosphothreonineCombined sources
Modified residuei1096 – 10961PhosphothreonineBy similarity
Modified residuei1110 – 11101PhosphoserineCombined sources
Modified residuei1193 – 11931PhosphoserineBy similarity
Modified residuei1220 – 12201PhosphoserineCombined sources
Isoform 2 (identifier: Q80TV8-2)
Modified residuei1139 – 11391PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ80TV8.
MaxQBiQ80TV8.
PaxDbiQ80TV8.
PeptideAtlasiQ80TV8.
PRIDEiQ80TV8.

PTM databases

iPTMnetiQ80TV8.
PhosphoSiteiQ80TV8.

Expressioni

Tissue specificityi

Highly expressed in brain and heart and at lower levels in kidney, lung, skeletal muscle and testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000064302.
CleanExiMM_CLASP1.

Interactioni

Subunit structurei

Interacts with ERC1, MAPRE1, MAPRE3, microtubules, and PHLDB2. The interaction with ERC1 may be mediated by PHLDB2. Interacts with GCC2; recruits CLASP1 to Golgi membranes (By similarity). Interacts with CLIP2 and RSN. Interacts with MACF1.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Clip2O551563EBI-908322,EBI-349416From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi218270. 5 interactions.
IntActiQ80TV8. 7 interactions.
MINTiMINT-4123448.
STRINGi10090.ENSMUSP00000042266.

Structurei

3D structure databases

ProteinModelPortaliQ80TV8.
SMRiQ80TV8. Positions 296-538, 849-1078.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati87 – 12438HEAT 1Add
BLAST
Repeati163 – 20038HEAT 2Add
BLAST
Repeati405 – 44036HEAT 3Add
BLAST
Repeati441 – 47737HEAT 4Add
BLAST
Repeati971 – 100838HEAT 5Add
BLAST
Repeati1339 – 137638HEAT 6Add
BLAST
Repeati1457 – 149438HEAT 7Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni662 – 782121Interaction with microtubules, MAPRE1 and MAPRE3By similarityAdd
BLAST
Regioni1251 – 1535285Interaction with CLIP2 and RSNAdd
BLAST
Regioni1251 – 1535285Interaction with PHLDB2By similarityAdd
BLAST
Regioni1253 – 1535283Localization to kinetochoresBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1296 – 132732Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi530 – 762233Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the CLASP family.Curated
Contains 7 HEAT repeats.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG2956. Eukaryota.
ENOG410ZMY0. LUCA.
HOVERGENiHBG079692.
InParanoidiQ80TV8.
KOiK16578.
PhylomeDBiQ80TV8.

Family and domain databases

Gene3Di1.25.10.10. 4 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR028399. CLASP_metazoan.
IPR024395. CLASP_N_dom.
IPR021133. HEAT_type_2.
[Graphical view]
PANTHERiPTHR21567:SF28. PTHR21567:SF28. 2 hits.
PfamiPF12348. CLASP_N. 2 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 5 hits.
PROSITEiPS50077. HEAT_REPEAT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80TV8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPRMESCLA QVLQKDVGKR LQVGQELIDY FSDRQKSADL EHDQTLLDKL
60 70 80 90 100
VDGLATSWVN SSNYKVVLLG MDILSALVTR LQDRFKAQIG TVLPSLIDRL
110 120 130 140 150
GDAKDSVREQ DQTLLLKIMD QAANPQYVWD RMLGGFKHKN FRTREGICLC
160 170 180 190 200
LIATLNASGA QTLTLSKIVP HICNLLGDPN SQVRDAAINS LVEIYRHVGE
210 220 230 240 250
RVRADLSKKG LPQSRLNVIF TKFDEVQKSG NMIQSANEKN FDDEDSVDGN
260 270 280 290 300
RPSSASSSSS KAPSSSRRNV NLGTTRRLMS SSLGSKSSAA KEGAGAVDEE
310 320 330 340 350
DFIKAFDDVP VVQIYSSRDL EESINKIREI LSDDKHDWEQ RVNALKKIRS
360 370 380 390 400
LLLAGAAEYD NFFQHLRLLD GAFKLSAKDL RSQVVREACI TLGHLSSVLG
410 420 430 440 450
NKFDHGAEAI MPTIFNLIPN SAKIMATSGV VAVRLIIRHT HIPRLIPVIT
460 470 480 490 500
SNCTSKSVAV RRRCFEFLDL LLQEWQTHSL ERHISVLAET IKKGIHDADS
510 520 530 540 550
EARIEARKCY WGFHSHFSRE AEHLYHTLES SYQKALQSHL KNSDSIVSLP
560 570 580 590 600
QSDRSSSSSQ ESLNRPLSAK RSPTGSTASR GSTVSTKSVS TTGSLQRSRS
610 620 630 640 650
DIDVNAAASA KSKVSSSSGS PAFSSAAALP PGSYASLGRI RTRRQSSGST
660 670 680 690 700
TNVASTPDSR GRSRAKVVSQ SQRSRSANPA GAGSRSSSPG KLLGSGLAGG
710 720 730 740 750
SSRGPPVTPS SEKRSKIPRS QGCSRETSPN RIGLARSSRI PRPSMSQGCS
760 770 780 790 800
RDTSRESSRD TSPARGFTPL DRFGLGQSGR IPGSVNAMRV LSTSTDLEAA
810 820 830 840 850
VADALKKPVR RRYEPYGMYS DDDANSDASS VCSERSYGSR NGGIPHYLRQ
860 870 880 890 900
TEDVAEVLNH CASSNWSERK EGLLGLQNLL KSQRTLSRVE LKRLCEIFTR
910 920 930 940 950
MFADPHSKRV FSMFLETLVD FIIIHKDDLQ DWLFVLLTQL LKKMGADLLG
960 970 980 990 1000
SVQAKVQKAL DVTRDSFPFD QQFNILMRFI VDQTQTPNLK VKVAILKYIE
1010 1020 1030 1040 1050
SLARQMDPTD FVNSSETRLA VSRIITWTTE PKSSDVRKAA QIVLISLFEL
1060 1070 1080 1090 1100
NTPEFTMLLG ALPKTFQDGA TKLLHNHLKN SSNTGVGSPS NTIGRTPSRH
1110 1120 1130 1140 1150
PSSRTSPLTS PTNCSHGGLS PSRLWGWSAD GLSKPPPPFS QPNSIPTAPS
1160 1170 1180 1190 1200
HKTLRRSYSP SMLDYDTENL NSEEIYSSLR GVTEAIEKFS FRSQEDLNEP
1210 1220 1230 1240 1250
IKRDGKKDCD IVSRDGGAAS PATEGRGGSE IEGGRMALDN KTSLLNTQPP
1260 1270 1280 1290 1300
RAFPGPRARE YNPYPYSDTI NTYDKTALKE AVFDDDMEQL RDVPIDHSDL
1310 1320 1330 1340 1350
VADLLKELSN HNERVEERKG ALLELLKITR EDSLGVWEEH FKTILLLLLE
1360 1370 1380 1390 1400
TLGDKDHSIR ALALRVLREI LRNQPARFKN YAELTIMKTL EAHKDSHKEV
1410 1420 1430 1440 1450
VRAAEEAAST LASSIHPEQC IKVLCPIIQT ADYPINLAAI KMQTKVVERI
1460 1470 1480 1490 1500
TKESLLQLLV DIIPGLLQGY DNTESSVRKA SVFCLVAIYS VIGEDLKPHL
1510 1520 1530
AQLTGSKMKL LNLYIKRAQT TNSNSSSSSD VSTHS
Length:1,535
Mass (Da):169,227
Last modified:January 23, 2007 - v2
Checksum:i5EE40CCE493C64D2
GO
Isoform 2 (identifier: Q80TV8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     770-770: L → LASRRHSRSTSALSTAESVGQS
     805-805: L → LLLGDARSK
     1120-1158: Missing.

Note: No experimental confirmation available.Combined sources
Show »
Length:1,525
Mass (Da):167,939
Checksum:iCA1BA686C4C8CB03
GO

Sequence cautioni

The sequence BAB24639 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC38020 differs from that shown.Intron retention.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1231 – 12311I → K in BAB24639 (PubMed:16141072).Curated
Sequence conflicti1417 – 14171P → S in AAH75708 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei770 – 7701L → LASRRHSRSTSALSTAESVG QS in isoform 2. 2 PublicationsVSP_022390
Alternative sequencei805 – 8051L → LLLGDARSK in isoform 2. 2 PublicationsVSP_022391
Alternative sequencei1120 – 115839Missing in isoform 2. 2 PublicationsVSP_022392Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC109294 Genomic DNA. No translation available.
AC131804 Genomic DNA. No translation available.
AC136636 Genomic DNA. No translation available.
AJ276962 mRNA. Translation: CAC35162.1.
AJ288061 mRNA. Translation: CAC35163.1.
AK006534 mRNA. Translation: BAB24639.2. Different initiation.
AK080782 mRNA. Translation: BAC38020.1. Sequence problems.
AK122330 mRNA. Translation: BAC65612.1.
BC057312 mRNA. Translation: AAH57312.1.
BC075708 mRNA. Translation: AAH75708.1.
BC094554 mRNA. Translation: AAH94554.1.
RefSeqiNP_001280229.1. NM_001293300.1.
NP_001280230.1. NM_001293301.1.
NP_083985.2. NM_029709.2.
UniGeneiMm.138740.

Genome annotation databases

GeneIDi76707.
KEGGimmu:76707.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC109294 Genomic DNA. No translation available.
AC131804 Genomic DNA. No translation available.
AC136636 Genomic DNA. No translation available.
AJ276962 mRNA. Translation: CAC35162.1.
AJ288061 mRNA. Translation: CAC35163.1.
AK006534 mRNA. Translation: BAB24639.2. Different initiation.
AK080782 mRNA. Translation: BAC38020.1. Sequence problems.
AK122330 mRNA. Translation: BAC65612.1.
BC057312 mRNA. Translation: AAH57312.1.
BC075708 mRNA. Translation: AAH75708.1.
BC094554 mRNA. Translation: AAH94554.1.
RefSeqiNP_001280229.1. NM_001293300.1.
NP_001280230.1. NM_001293301.1.
NP_083985.2. NM_029709.2.
UniGeneiMm.138740.

3D structure databases

ProteinModelPortaliQ80TV8.
SMRiQ80TV8. Positions 296-538, 849-1078.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi218270. 5 interactions.
IntActiQ80TV8. 7 interactions.
MINTiMINT-4123448.
STRINGi10090.ENSMUSP00000042266.

PTM databases

iPTMnetiQ80TV8.
PhosphoSiteiQ80TV8.

Proteomic databases

EPDiQ80TV8.
MaxQBiQ80TV8.
PaxDbiQ80TV8.
PeptideAtlasiQ80TV8.
PRIDEiQ80TV8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi76707.
KEGGimmu:76707.

Organism-specific databases

CTDi23332.
MGIiMGI:1923957. Clasp1.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG2956. Eukaryota.
ENOG410ZMY0. LUCA.
HOVERGENiHBG079692.
InParanoidiQ80TV8.
KOiK16578.
PhylomeDBiQ80TV8.

Miscellaneous databases

PROiQ80TV8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000064302.
CleanExiMM_CLASP1.

Family and domain databases

Gene3Di1.25.10.10. 4 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR028399. CLASP_metazoan.
IPR024395. CLASP_N_dom.
IPR021133. HEAT_type_2.
[Graphical view]
PANTHERiPTHR21567:SF28. PTHR21567:SF28. 2 hits.
PfamiPF12348. CLASP_N. 2 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 5 hits.
PROSITEiPS50077. HEAT_REPEAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLAP1_MOUSE
AccessioniPrimary (citable) accession number: Q80TV8
Secondary accession number(s): Q505G6
, Q6DI75, Q6PG14, Q8BNS4, Q99JH5, Q99JI2, Q9CVU1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.