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Protein

Cullin-9

Gene

Cul9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Core component of a Cul9-RING ubiquitin-protein ligase complex, a complex that mediates ubiquitination and subsequent degradation of BIRC5 and is required to maintain microtubule dynamics and genome integrity. Acts downstream of the 3M complex, which inhibits CUL9 activity, leading to prevent ubiquitination of BIRC5 (PubMed:24793696). Cytoplasmic anchor protein in p53/TP53-associated protein complex. Regulates the subcellular localization of p53/TP53 and subsequent function.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi700 – 707ATPSequence analysis8
Zinc fingeri1409 – 1459RING-type 1; atypicalPROSITE-ProRule annotationAdd BLAST51
Zinc fingeri1479 – 1542IBR-typeAdd BLAST64
Zinc fingeri1575 – 1604RING-type 2PROSITE-ProRule annotationAdd BLAST30

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Cullin-9
Short name:
CUL-9
Alternative name(s):
p53-associated parkin-like cytoplasmic protein
Gene namesi
Name:Cul9
Synonyms:Kiaa0708, Parc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1925559. Cul9.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mice were born at the expected Mendelian ratio and do not show apparent phenotype. They are however more susceptible to carcinogenesis and develop spontaneous tumor. Cells display polyploidy and aneuploidy.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001198161 – 1865Cullin-9Add BLAST1865

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei313PhosphoserineBy similarity1
Modified residuei794PhosphoserineBy similarity1
Modified residuei1775PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ80TT8.
PeptideAtlasiQ80TT8.
PRIDEiQ80TT8.

Expressioni

Gene expression databases

CleanExiMM_PARC.

Interactioni

Subunit structurei

Part of a Cul9-RING complex at least composed of CUL9 and RBX1. Interacts with CUL7: interaction with CUL7 component of the 3M complex leads to inhibition of CUL9 activity. The CUL7-CUL9 heterodimer seems to interact specifically with TP53. Forms a complex with p53/TP53 in the cytoplasm of unstressed cells. Interacts with UBCH7 and UBCH8 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000067736.

Structurei

3D structure databases

ProteinModelPortaliQ80TT8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini480 – 659DOCPROSITE-ProRule annotationAdd BLAST180

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1794 – 1835Sequence analysisAdd BLAST42

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1426 – 1435Cys-rich10
Compositional biasi1800 – 1862Glu-richAdd BLAST63

Domaini

The IBR domain is required for interaction with UBCH7 and UBCH8.

Sequence similaritiesi

Belongs to the cullin family.PROSITE-ProRule annotation
Contains 1 DOC domain.PROSITE-ProRule annotation
Contains 1 IBR-type zinc finger.Curated
Contains 2 RING-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1409 – 1459RING-type 1; atypicalPROSITE-ProRule annotationAdd BLAST51
Zinc fingeri1479 – 1542IBR-typeAdd BLAST64
Zinc fingeri1575 – 1604RING-type 2PROSITE-ProRule annotationAdd BLAST30

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1815. Eukaryota.
ENOG410XP9Y. LUCA.
HOGENOMiHOG000024831.
InParanoidiQ80TT8.
PhylomeDBiQ80TT8.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
InterProiIPR004939. APC_su10/DOC_dom.
IPR016024. ARM-type_fold.
IPR031222. CUL9.
IPR016157. Cullin_CS.
IPR016158. Cullin_homology.
IPR001373. Cullin_N.
IPR019559. Cullin_neddylation_domain.
IPR008979. Galactose-bd-like.
IPR002867. IBR_dom.
IPR001841. Znf_RING.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERiPTHR22771:SF2. PTHR22771:SF2. 1 hit.
PfamiPF03256. ANAPC10. 1 hit.
PF00888. Cullin. 1 hit.
PF10557. Cullin_Nedd8. 1 hit.
PF01485. IBR. 2 hits.
[Graphical view]
SMARTiSM01337. APC10. 1 hit.
SM00884. Cullin_Nedd8. 1 hit.
SM00647. IBR. 2 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF75632. SSF75632. 2 hits.
PROSITEiPS01256. CULLIN_1. 1 hit.
PS50069. CULLIN_2. 1 hit.
PS51284. DOC. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80TT8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARALRGPGP RSSLDQHVAA VLATVQISSL DTNLQLSGLC ALSQAVEEVT
60 70 80 90 100
ERDHPLVRPD RPLREKLVKT LVDLLTNQVG EKMVVVMALR LLYLLMTKHE
110 120 130 140 150
WRPLFAREGG IYAVLICMQE YKTSVLVQQA GLAALKMLAI ANSSDVPTFI
160 170 180 190 200
PSRDSIQPLF DTQMTREIFA SIDSATRPGS ESLLLSVPAA VVLMLNTEGC
210 220 230 240 250
SSAVRNGLLL LNLLLCNHHT LGDQIITQEL RDTLFRHSGI APGTEPMPTT
260 270 280 290 300
RTILTMLLSR YSEPRGSPER AVLETPSTQG QDGSPESLIR SLVGDLSAEL
310 320 330 340 350
FLDLERVLCH EGSPPAAVRP LLRRLQQETQ PFLLLLRTLD APGPNRTLLL
360 370 380 390 400
TILRVMTRLL DHPETMVLPW HEVLEPCLNC LNGPSSDSEV VQEVTCFLHR
410 420 430 440 450
LALMQKDYAV VLCCLGGKEA LSKVLDKHST QLLLASELRH LVAECEKYSQ
460 470 480 490 500
LCSNLTSSIL AGCIQMVLGQ IEDHRRTYKP ITIPFFDVFL RHLCQGSSVE
510 520 530 540 550
VKEDRCWEKV EVSSNPHRAS KLTDRNPKTY WESNGSTGSH SITLHMHRGV
560 570 580 590 600
LIRQLTLLVA SEDSSYMPAR VVVFGGDNVG CISTELNTVN VMPSASRVTL
610 620 630 640 650
LENLTRFWPI IQIRIKRCQQ GGIDTRVRGV EVLGPKPTFW PLFREQLCRR
660 670 680 690 700
TCLFYTIRAQ AWSRDIAEDR QRLLQLYPRL NRVLRHEQNF ADRFLPDDEA
710 720 730 740 750
AQALGKTCWE ALVSPLVQNI TSPDAGGVSS LGWLLDQYLE HRESTRSRQG
760 770 780 790 800
PAASFASQVR RLCHLLVHVE APPGPSPEPS SQPLSKNSKG QDGSPTPAPT
810 820 830 840 850
PVCPSTSLRN LTQCWLSVVQ GQVSRFLAAA WRASDFVPRY CSLYQRLQSA
860 870 880 890 900
GSELFGPRAA FTLALRSGFS GALLQQSFLT AAHISEQFAR HIDQQIHGGL
910 920 930 940 950
LGGAAGVGML GRLQRHLEPI MVLSGLELAT TFEHFYQHYM ADRLLSLGSS
960 970 980 990 1000
WLEGAVLEQI GPCFPNRLPQ LMLQSLHTSE ELQHRFHLFQ LQQLDRQLLE
1010 1020 1030 1040 1050
QGDQEEWRPE KVEEDDEGQE TGRELFTDPG PAISVLVLSP RCWPVSPLCY
1060 1070 1080 1090 1100
LHHPRKHLQA ELCDALERFS SFYSHSQNCP VLDIGPHRRL QWTWLGRAEL
1110 1120 1130 1140 1150
QLGDQTLHVS LNTVQMWLLF NQSEEVSVET LLRNSDLSPE LLHQALLPLT
1160 1170 1180 1190 1200
SDNGPLTLEE TQDYPQGGVL RLREPRSQTH EEFLWLIPPQ TYLSVEKDEG
1210 1220 1230 1240 1250
RTLEQKRNLL SCLLVRILKA HREKGLHIDQ LVCLVLEAWQ KGPDPPGRLG
1260 1270 1280 1290 1300
NAAAVGVACS STDVLSCILH LLGQGYVERR DDRPQVLMYA TPEPMGPCRG
1310 1320 1330 1340 1350
QADVPFCGNK NTETSRPSPE AVVALASLQL PAGRTMSPQE VEGLMEQTVR
1360 1370 1380 1390 1400
QVQETLNLEP DVAQHLLAHS HWGTEQLLQS YSDDPEPLLL AAGLRVPQAQ
1410 1420 1430 1440 1450
VVPTRPDQCP VCVTPLGPHD DSPSLCCLHC CCKSCWNEYL TTRIEQNFVL
1460 1470 1480 1490 1500
NCTCPIADCP AQPTGAFIRN IVSSPEVISK YEKALLRGYV ESCSNLTWCT
1510 1520 1530 1540 1550
NPQGCDRILC RQGLGSGTTC SKCGWASCFS CSFPEAHYPA SCGHMSQWVD
1560 1570 1580 1590 1600
DGGYYDGMSV EAQSKHLAKL ISKRCPSCQA PIEKNEGCLH MTCARCNHGF
1610 1620 1630 1640 1650
CWRCLKSWKP SHKDYYNCSA MVSKAARQEK RFQDYNERCT FHHQAREFAV
1660 1670 1680 1690 1700
NLRNQASAIQ EVPPPKSFTF LQDACRALEQ ARKVLAYACV YSFYSQDTEY
1710 1720 1730 1740 1750
MDVVEQQTEN LELHTNALQI LLEETLLRCR DLASSLRFLR ADCLSTGTEL
1760 1770 1780 1790 1800
LRRIQERLLA ILQHSTQDFR VGLQSPSVET REVKGSNVPS DQPQGSSGLE
1810 1820 1830 1840 1850
VEDEEEEEEE EEEEEEEEEE DVPEWQHEFD EELDNDSFSY DEESENLDRE
1860
TFFFGDEDED DESYD
Length:1,865
Mass (Da):209,160
Last modified:March 1, 2004 - v2
Checksum:i9956A32BE5BC5803
GO
Isoform 2 (identifier: Q80TT8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-908: Missing.

Note: No experimental confirmation available.
Show »
Length:957
Mass (Da):108,659
Checksum:i7344868C43F23A9B
GO
Isoform 3 (identifier: Q80TT8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     134-144: ALKMLAIANSS → VSTLGRAGQSD
     145-1865: Missing.

Note: No experimental confirmation available.
Show »
Length:144
Mass (Da):15,833
Checksum:i6975CFF80F6A72D9
GO
Isoform 4 (identifier: Q80TT8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     944-1009: LLSLGSSWLE...EQGDQEEWRP → RLEENSLQTQ...VRSWGQEAGA
     1010-1865: Missing.

Note: No experimental confirmation available.
Show »
Length:1,009
Mass (Da):111,882
Checksum:i85D860EC1C6BA8D4
GO

Sequence cautioni

The sequence AK051474 differs from that shown. Reason: Frameshift at position 1664.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1102L → F in AK051474 (PubMed:16141072).Curated1
Sequence conflicti1104 – 1119Missing in BAC65633 (PubMed:12693553).CuratedAdd BLAST16
Sequence conflicti1111 – 1119LNTVQMWLL → TVQMWLLLN in AK051474 (PubMed:16141072).Curated9
Sequence conflicti1183F → V in AK051474 (PubMed:16141072).Curated1
Sequence conflicti1592T → N in AK051474 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0095761 – 908Missing in isoform 2. 1 PublicationAdd BLAST908
Alternative sequenceiVSP_009577134 – 144ALKMLAIANSS → VSTLGRAGQSD in isoform 3. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_009578145 – 1865Missing in isoform 3. 1 PublicationAdd BLAST1721
Alternative sequenceiVSP_009579944 – 1009LLSLG…EEWRP → RLEENSLQTQDQPFPSWSCH RAAGRSPHCAICTTPESTYR RSSAMPWSASPASTATVRSW GQEAGA in isoform 4. 1 PublicationAdd BLAST66
Alternative sequenceiVSP_0095801010 – 1865Missing in isoform 4. 1 PublicationAdd BLAST856

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK051474 mRNA. No translation available.
BC026469 mRNA. Translation: AAH26469.1.
BC041674 mRNA. Translation: AAH41674.1.
AK122351 Transcribed RNA. Translation: BAC65633.1.
UniGeneiMm.329076.

Genome annotation databases

UCSCiuc008ctb.2. mouse. [Q80TT8-1]
uc008ctf.2. mouse. [Q80TT8-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK051474 mRNA. No translation available.
BC026469 mRNA. Translation: AAH26469.1.
BC041674 mRNA. Translation: AAH41674.1.
AK122351 Transcribed RNA. Translation: BAC65633.1.
UniGeneiMm.329076.

3D structure databases

ProteinModelPortaliQ80TT8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000067736.

Proteomic databases

PaxDbiQ80TT8.
PeptideAtlasiQ80TT8.
PRIDEiQ80TT8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiuc008ctb.2. mouse. [Q80TT8-1]
uc008ctf.2. mouse. [Q80TT8-3]

Organism-specific databases

MGIiMGI:1925559. Cul9.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1815. Eukaryota.
ENOG410XP9Y. LUCA.
HOGENOMiHOG000024831.
InParanoidiQ80TT8.
PhylomeDBiQ80TT8.

Miscellaneous databases

PROiQ80TT8.
SOURCEiSearch...

Gene expression databases

CleanExiMM_PARC.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
InterProiIPR004939. APC_su10/DOC_dom.
IPR016024. ARM-type_fold.
IPR031222. CUL9.
IPR016157. Cullin_CS.
IPR016158. Cullin_homology.
IPR001373. Cullin_N.
IPR019559. Cullin_neddylation_domain.
IPR008979. Galactose-bd-like.
IPR002867. IBR_dom.
IPR001841. Znf_RING.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERiPTHR22771:SF2. PTHR22771:SF2. 1 hit.
PfamiPF03256. ANAPC10. 1 hit.
PF00888. Cullin. 1 hit.
PF10557. Cullin_Nedd8. 1 hit.
PF01485. IBR. 2 hits.
[Graphical view]
SMARTiSM01337. APC10. 1 hit.
SM00884. Cullin_Nedd8. 1 hit.
SM00647. IBR. 2 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF75632. SSF75632. 2 hits.
PROSITEiPS01256. CULLIN_1. 1 hit.
PS50069. CULLIN_2. 1 hit.
PS51284. DOC. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCUL9_MOUSE
AccessioniPrimary (citable) accession number: Q80TT8
Secondary accession number(s): Q8BKL9, Q8CGC0, Q8R363
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: November 2, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.