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Protein

Pleckstrin homology domain-containing family M member 2

Gene

Plekhm2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in the regulation of conventional kinesin activity. Required for maintenance of the Golgi apparatus organization. May play a role in membrane tubulation. May play a role in lysosomes movement and localization at the cell periphery.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Pleckstrin homology domain-containing family M member 2
Short name:
PH domain-containing family M member 2
Gene namesi
Name:Plekhm2
Synonyms:Kiaa0842
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1916832. Plekhm2.

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003094561 – 1018Pleckstrin homology domain-containing family M member 2Add BLAST1018

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei423PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ80TQ5.
PeptideAtlasiQ80TQ5.
PRIDEiQ80TQ5.

PTM databases

iPTMnetiQ80TQ5.
PhosphoSitePlusiQ80TQ5.

Interactioni

Subunit structurei

Interacts with KIF5B (By similarity). Interacts with BORCS5 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000081221.

Structurei

3D structure databases

ProteinModelPortaliQ80TQ5.
SMRiQ80TQ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 158RUNPROSITE-ProRule annotationAdd BLAST123
Domaini770 – 872PHPROSITE-ProRule annotationAdd BLAST103

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 289Interaction with KIF5BBy similarityAdd BLAST289

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi296 – 299Poly-Lys4
Compositional biasi302 – 307Poly-Lys6
Compositional biasi485 – 488Poly-Glu4
Compositional biasi592 – 595Poly-Leu4

Sequence similaritiesi

Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 RUN domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IFVN. Eukaryota.
ENOG410ZZUG. LUCA.
HOGENOMiHOG000231299.
HOVERGENiHBG059073.
InParanoidiQ80TQ5.
KOiK15348.
TreeFamiTF332641.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR004012. Run_dom.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF02759. RUN. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00593. RUN. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50826. RUN. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80TQ5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPREVKDRI LENISLSVKK LQSYFAACED ETPAIRNHDK VLQRLCEHLD
60 70 80 90 100
HALLYGLQDL SSGYWVLVVH FTRREAIRQI EVLQHVATNL GRSRAWLYLA
110 120 130 140 150
LNENSLESYL RLFQENLGLL QKYYVRNALV CSHDHLTLFL TLVSGLEFIR
160 170 180 190 200
FDLDLDAPYL DLAPYMPDYY KPQYLLDFED RLPSSVHGSD SLSLNSFNSV
210 220 230 240 250
TSTNLEWDDS AIAPSSEDGD LTDTISGPRS TASDLTSSKT STKSPTQRHN
260 270 280 290 300
PFNEEQAETA SSDTTPVHTT SQEKEEAQAP DQPDACTELE VIRVTKKKKI
310 320 330 340 350
GKKKKTKLDE DASPLHPTSS QQKCGQQGEG DGLVGTPGLA RDPSDTVLAS
360 370 380 390 400
PQEQGEGLSS TAGSSELSEL SQMGLLIPEM KDTSMECLGQ PLSKVIDKLH
410 420 430 440 450
GQLDPSTWCS HADPPEQSFR AGSPGEAPEK PPFCDFSEGL PAPMDFYRFT
460 470 480 490 500
VESPSTVAPG GGHHDPPGPS QPLHVPGSPA AALQEEEEGG RGEGQTSQPV
510 520 530 540 550
EDRQGEEIQE PEPQEPDSQL PLVSQEPLVS QEPVPEPVSQ PEPGTHEALC
560 570 580 590 600
KLKRDQPSPC LSSAEDSGVE EGQGSPSEMT HPSEFRVDNN HLLLLMIHVF
610 620 630 640 650
RENEEQLFKM IRMSTGHMEG NLQLLYVLLT DCYVYLLRKG ATEKPYLVEE
660 670 680 690 700
AVSYNELDYV SVGLDQQTVK LVCTNRRKQF LLDTADVALA ELFLASLKSA
710 720 730 740 750
MIKGCREPPY PSILTDATME KLALAKFVAQ ESKCEASAVT VHFYGLVHWE
760 770 780 790 800
DPMEEALGPV PCQCSPAEGT ITKEGMLHYK ASTSYLGKEH WKACFVVLSN
810 820 830 840 850
GILYQYPDRT DVIPLLSVNM GGEQCGGCRR SNTTDRPHAF QVILADRPCL
860 870 880 890 900
ELSADSEAEM ADWMQHLCQA VSKGVIPQGI APSPCIPCCL VITEDRLFTC
910 920 930 940 950
HEDCQTSFFR SLGTARLADI TAISTELGKE YCVLEFSQDS PQLLQPWVIY
960 970 980 990 1000
LSCTSELDRF LTALSSGWKA IYQVDLPHKA IHEASIKQKF EDALSLIHSA
1010
WQRSDSLCRG RASRDPWC
Length:1,018
Mass (Da):112,734
Last modified:November 13, 2007 - v2
Checksum:i1024AD61CE90767D
GO
Isoform 2 (identifier: Q80TQ5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     217-217: E → EDYDFGDVFPAVPSVPSTDWE
     527-532: Missing.

Note: No experimental confirmation available.
Show »
Length:1,032
Mass (Da):114,305
Checksum:i2462B24EA0F8F9A6
GO
Isoform 3 (identifier: Q80TQ5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-165: Missing.
     217-217: E → EDYDFGDVFPAVPSVPSTDWE
     527-532: Missing.

Note: No experimental confirmation available.
Show »
Length:867
Mass (Da):95,047
Checksum:iDED74330FEB5D382
GO

Sequence cautioni

The sequence BAC65668 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti490G → GG in CAM19908 (PubMed:19468303).Curated1
Sequence conflicti490G → GG in AAH58984 (PubMed:15489334).Curated1
Sequence conflicti500V → L in CAM19908 (PubMed:19468303).Curated1
Sequence conflicti500V → L in AAH58984 (PubMed:15489334).Curated1
Sequence conflicti612R → L in AAH58984 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0291621 – 165Missing in isoform 3. 1 PublicationAdd BLAST165
Alternative sequenceiVSP_029163217E → EDYDFGDVFPAVPSVPSTDW E in isoform 2 and isoform 3. 2 Publications1
Alternative sequenceiVSP_029164527 – 532Missing in isoform 2 and isoform 3. 2 Publications6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122386 mRNA. Translation: BAC65668.1. Different initiation.
AK156652 mRNA. Translation: BAE33794.1.
AL670446 Genomic DNA. Translation: CAM19908.1.
BC058984 mRNA. Translation: AAH58984.1.
RefSeqiNP_001028322.1. NM_001033150.1.
UniGeneiMm.33220.

Genome annotation databases

GeneIDi69582.
KEGGimmu:69582.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122386 mRNA. Translation: BAC65668.1. Different initiation.
AK156652 mRNA. Translation: BAE33794.1.
AL670446 Genomic DNA. Translation: CAM19908.1.
BC058984 mRNA. Translation: AAH58984.1.
RefSeqiNP_001028322.1. NM_001033150.1.
UniGeneiMm.33220.

3D structure databases

ProteinModelPortaliQ80TQ5.
SMRiQ80TQ5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000081221.

PTM databases

iPTMnetiQ80TQ5.
PhosphoSitePlusiQ80TQ5.

Proteomic databases

PaxDbiQ80TQ5.
PeptideAtlasiQ80TQ5.
PRIDEiQ80TQ5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi69582.
KEGGimmu:69582.

Organism-specific databases

CTDi23207.
MGIiMGI:1916832. Plekhm2.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IFVN. Eukaryota.
ENOG410ZZUG. LUCA.
HOGENOMiHOG000231299.
HOVERGENiHBG059073.
InParanoidiQ80TQ5.
KOiK15348.
TreeFamiTF332641.

Miscellaneous databases

ChiTaRSiPlekhm2. mouse.
PROiQ80TQ5.
SOURCEiSearch...

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR004012. Run_dom.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF02759. RUN. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00593. RUN. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50826. RUN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPKHM2_MOUSE
AccessioniPrimary (citable) accession number: Q80TQ5
Secondary accession number(s): A2ADE1, Q3U0Q7, Q6PD22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: November 13, 2007
Last modified: November 2, 2016
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.