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Q80TN5 (ZDH17_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Palmitoyltransferase ZDHHC17

EC=2.3.1.225
Alternative name(s):
Huntingtin-interacting protein 14
Zinc finger DHHC domain-containing protein 17
Short name=DHHC-17
Gene names
Name:Zdhhc17
Synonyms:Hip14, Kiaa0946
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length632 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Palmitoyltransferase specific for a subset of neuronal proteins, including SNAP25, DLG4/PSD95, GAD2, SYT1 and HD. Palmitoylates MPP1 in erythrocytes. May be involved in the sorting or targeting of critical proteins involved in the initiating events of endocytosis at the plasma membrane. Has transforming activity. Mediates Mg2+ transport. Ref.5 Ref.6

Catalytic activity

Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA.

Subunit structure

Interacts (via ANK repeats) with HD By similarity.

Subcellular location

Golgi apparatus membrane; Multi-pass membrane protein By similarity. Cytoplasmic vesicle membrane; Multi-pass membrane protein By similarity. Note: Localized predominantly in the perinuclear regions of neurons from the cortex, striatum and hippocampus. Colocalized with HD in the medium spiny neurons of the striatum and the spiny neurons that project into the globus pallidus. Ref.4

Tissue specificity

Expressed in liver, testis, kidney, heart, pancreas and brain. Highest expression was seen in the brain. Ref.4

Induction

Up-regulated by low extracellular Mg2+. Ref.7

Domain

The DHHC domain is required for palmitoyltransferase activity By similarity.

Post-translational modification

Autopalmitoylated. Autopalmitoylation has a regulatory role in ZDHHC17-mediated Mg2+ transport By similarity.

Sequence similarities

Belongs to the DHHC palmitoyltransferase family. AKR/ZDHHC17 subfamily.

Contains 5 ANK repeats.

Contains 1 DHHC-type zinc finger.

Sequence caution

The sequence AAH58772.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

The sequence BAC30090.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAC30097.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAC32912.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAC65688.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
   Biological processIon transport
Transport
   Cellular componentCytoplasmic vesicle
Golgi apparatus
Membrane
   Coding sequence diversityAlternative splicing
   DiseaseTumor suppressor
   DomainANK repeat
Repeat
Transmembrane
Transmembrane helix
Zinc-finger
   LigandMagnesium
Metal-binding
Zinc
   Molecular functionAcyltransferase
Transferase
   PTMLipoprotein
Palmitate
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processlipoprotein transport

Inferred from electronic annotation. Source: Ensembl

positive regulation of I-kappaB kinase/NF-kappaB signaling

Inferred from electronic annotation. Source: Ensembl

protein palmitoylation

Inferred from direct assay Ref.5. Source: MGI

   Cellular_componentGolgi membrane

Inferred from sequence or structural similarity. Source: UniProtKB

Golgi-associated vesicle membrane

Inferred from sequence or structural similarity. Source: UniProtKB

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionmagnesium ion transmembrane transporter activity

Inferred from sequence or structural similarity. Source: UniProtKB

palmitoyltransferase activity

Inferred from direct assay Ref.5. Source: MGI

protein-cysteine S-palmitoyltransferase activity

Inferred from sequence or structural similarity. Source: UniProtKB

signal transducer activity

Inferred from electronic annotation. Source: Ensembl

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q80TN5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q80TN5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     423-429: TIVELAE → VVMYPAN
     430-632: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 632632Palmitoyltransferase ZDHHC17
PRO_0000212901

Regions

Topological domain1 – 304304Cytoplasmic Potential
Transmembrane305 – 32521Helical; Potential
Transmembrane326 – 34621Helical; Potential
Topological domain347 – 35711Cytoplasmic Potential
Transmembrane358 – 37821Helical; Potential
Topological domain379 – 3813Lumenal Potential
Transmembrane382 – 40221Helical; Potential
Topological domain403 – 48078Cytoplasmic Potential
Transmembrane481 – 50121Helical; Potential
Topological domain502 – 52928Lumenal Potential
Transmembrane530 – 55021Helical; Potential
Topological domain551 – 63282Cytoplasmic Potential
Repeat89 – 11830ANK 1
Repeat123 – 15230ANK 2
Repeat156 – 18530ANK 3
Repeat189 – 21931ANK 4
Repeat224 – 25330ANK 5
Zinc finger437 – 48751DHHC-type

Sites

Active site4671S-palmitoyl cysteine intermediate By similarity

Natural variations

Alternative sequence423 – 4297TIVELAE → VVMYPAN in isoform 2.
VSP_010026
Alternative sequence430 – 632203Missing in isoform 2.
VSP_010027

Experimental info

Sequence conflict2881N → K in BAC32912. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 13, 2004. Version 2.
Checksum: 323EFD47790750DC

FASTA63272,621
        10         20         30         40         50         60 
MQREEGFNTK MADGPDEYET ETGCVPLLHP EEIKPQSHYN HGYGEPLGRK THIDDYSTWD 

        70         80         90        100        110        120 
IVKATQYGIY ERCRELVEAG YDVRQPDKEN VTLLHWAAIN NRIDLVKYYI SKGAIVDQLG 

       130        140        150        160        170        180 
GDLNSTPLHW ATRQGHLSMV VQLMKYGADP SLIDGEGCSC IHLAAQFGHT SIVAYLIAKG 

       190        200        210        220        230        240 
QDVDMMDQNG MTPLMWAAYR THSVDPTRLL LTFNVSVNLG DKYHKNTALH WAVLAGNTTV 

       250        260        270        280        290        300 
ISLLLEAGGN VDAQNVKGES ALDLAKQRKN VWMINHLQEA RQAKGYDNPS FLRKLKADKE 

       310        320        330        340        350        360 
FRQKVMLGTP FLVIWLVGFI ADLDIDSWLI KGLMYGGVWA TVQFLSKSFF DHSMHSALPL 

       370        380        390        400        410        420 
GIYLATKFWM YVTWFFWFWN DLNFLFIHLP FLANSVALFY NFGKSWKSDP GIIKATEEQK 

       430        440        450        460        470        480 
KKTIVELAET GSLDLSIFCS TCLIRKPVRS KHCGVCNRCI AKFDHHCPWV GNCVGAGNHR 

       490        500        510        520        530        540 
YFMGYLFFLL FMICWMIYGC VSYWGLHCET TYTKDGFWTY ITQIATCSPW MFWMFLNSVF 

       550        560        570        580        590        600 
HFLWVAVLLM CQLYQITCLG ITTNERMNAR RYKHFKVTTT SIESPFNHGC VRNIIDFFEF 

       610        620        630 
RCCGLFRPVI VDWTRQYTIE YDQISGSGYQ LV 

« Hide

Isoform 2 [UniParc].

Checksum: FE0E0E96934E4FC9
Show »

FASTA42948,784

References

« Hide 'large scale' references
[1]"Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 8-632 (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Cerebellum and Hypothalamus.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 9-418.
Tissue: Eye.
[4]"HIP14, a novel ankyrin domain-containing protein, links huntingtin to intracellular trafficking and endocytosis."
Singaraja R.R., Hadano S., Metzler M., Givan S., Wellington C.L., Warby S., Yanai A., Gutekunst C.-A., Leavitt B.R., Yi H., Fichter K., Gan L., McGutcheon K., Chopra V., Michel J., Hersch S.M., Ikeda J.E., Hayden M.R.
Hum. Mol. Genet. 11:2815-2828(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[5]"Identification of PSD-95 palmitoylating enzymes."
Fukata M., Fukata Y., Adesnik H., Nicoll R.A., Bredt D.S.
Neuron 44:987-996(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Huntingtin interacting protein 14 is an oncogenic human protein: palmitoyl acyltransferase."
Ducker C.E., Stettler E.M., French K.J., Upson J.J., Smith C.D.
Oncogene 23:9230-9237(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Huntingtin-interacting proteins, HIP14 and HIP14L, mediate dual functions, palmitoyl acyltransferase and Mg2+ transport."
Goytain A., Hines R.M., Quamme G.A.
J. Biol. Chem. 283:33365-33374(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK122406 mRNA. Translation: BAC65688.1. Different initiation.
AK038670 mRNA. Translation: BAC30090.2. Different initiation.
AK038681 mRNA. Translation: BAC30097.2. Different initiation.
AK046891 mRNA. Translation: BAC32912.1. Different initiation.
BC058772 mRNA. Translation: AAH58772.1. Sequence problems.
CCDSCCDS56748.1. [Q80TN5-1]
RefSeqNP_766142.2. NM_172554.2. [Q80TN5-1]
UniGeneMm.339281.

3D structure databases

ProteinModelPortalQ80TN5.
SMRQ80TN5. Positions 21-281.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ80TN5.

Proteomic databases

PaxDbQ80TN5.
PRIDEQ80TN5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000041723; ENSMUSP00000043279; ENSMUSG00000035798. [Q80TN5-1]
GeneID320150.
KEGGmmu:320150.
UCSCuc007gzu.2. mouse. [Q80TN5-1]
uc007gzw.2. mouse. [Q80TN5-2]

Organism-specific databases

CTD23390.
MGIMGI:2445110. Zdhhc17.
RougeSearch...

Phylogenomic databases

eggNOGCOG0666.
GeneTreeENSGT00530000063074.
HOGENOMHOG000290181.
HOVERGENHBG051907.
InParanoidQ80TN5.
OMAFWNDAHL.
OrthoDBEOG70S74V.
PhylomeDBQ80TN5.
TreeFamTF317342.

Gene expression databases

ArrayExpressQ80TN5.
BgeeQ80TN5.
CleanExMM_ZDHHC17.
GenevestigatorQ80TN5.

Family and domain databases

Gene3D1.25.40.20. 1 hit.
InterProIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001594. Znf_DHHC_palmitoyltrfase.
[Graphical view]
PfamPF12796. Ank_2. 3 hits.
PF01529. zf-DHHC. 1 hit.
[Graphical view]
PRINTSPR01415. ANKYRIN.
SMARTSM00248. ANK. 5 hits.
[Graphical view]
SUPFAMSSF48403. SSF48403. 1 hit.
PROSITEPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 5 hits.
PS50216. ZF_DHHC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio396133.
PROQ80TN5.
SOURCESearch...

Entry information

Entry nameZDH17_MOUSE
AccessionPrimary (citable) accession number: Q80TN5
Secondary accession number(s): Q8BJ08, Q8BYQ2, Q8BYQ6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: July 9, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot