Q80TN5 (ZDH17_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Palmitoyltransferase ZDHHC17
EC=2.3.1.-

Alternative name(s):
Huntingtin-interacting protein 14
Zinc finger DHHC domain-containing protein 17
Short name=DHHC-17

Gene names

Name:	Zdhhc17
Synonyms:	Hip14, Kiaa0946

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	632 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Palmitoyltransferase specific for a subset of neuronal proteins, including SNAP25, DLG4/PSD95, GAD2, SYT1 and HD. Palmitoylates MPP1 in erythrocytes. May be involved in the sorting or targeting of critical proteins involved in the initiating events of endocytosis at the plasma membrane. Has transforming activity. Mediates Mg2+ transport By similarity. Ref.5 Ref.6
Catalytic activity	Palmitoyl-CoA + protein-cysteine = S-palmitoyl protein + CoA.
Subunit structure	Interacts (via ANK repeats) with HD By similarity.
Subcellular location	Golgi apparatus membrane; Multi-pass membrane protein By similarity. Cytoplasmic vesicle membrane; Multi-pass membrane protein By similarity. Note: Localized predominantly in the perinuclear regions of neurons from the cortex, striatum and hippocampus. Colocalized with HD in the medium spiny neurons of the striatum and the spiny neurons that project into the globus pallidus. Ref.4
Tissue specificity	Expressed in liver, testis, kidney, heart, pancreas and brain. Highest expression was seen in the brain. Ref.4
Induction	Up-regulated by low extracellular Mg2+. Ref.7
Domain	The DHHC domain is required for palmitoyltransferase activity By similarity.
Post-translational modification	Autopalmitoylated. Autopalmitoylation has a regulatory role in ZDHHC17-mediated Mg2+ transport By similarity.
Sequence similarities	Belongs to the DHHC palmitoyltransferase family. AKR/ZDHHC17 subfamily. Contains 5 ANK repeats. Contains 1 DHHC-type zinc finger.
Sequence caution	The sequence AAH58772.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence BAC30090.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAC30097.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAC32912.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAC65688.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cytoplasmic vesicle Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Disease	Tumor suppressor
Domain	ANK repeat Repeat Transmembrane Transmembrane helix Zinc-finger
Ligand	Magnesium Metal-binding Zinc
Molecular function	Acyltransferase Transferase
PTM	Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lipoprotein transport Inferred from electronic annotation. Source: Compara positive regulation of I-kappaB kinase/NF-kappaB cascade Inferred from electronic annotation. Source: Compara protein palmitoylation Inferred from direct assay Ref.5. Source: MGI
Cellular_component	Golgi-associated vesicle membrane Inferred from sequence or structural similarity. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	magnesium ion transmembrane transporter activity Inferred from sequence or structural similarity. Source: UniProtKB protein-cysteine S-palmitoleyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB signal transducer activity Inferred from electronic annotation. Source: Compara zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q80TN5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q80TN5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     423-429: TIVELAE → VVMYPAN
     430-632: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 632	632	Palmitoyltransferase ZDHHC17		PRO_0000212901
Regions
	Topological domain	1 – 304	304	Cytoplasmic Potential
	Transmembrane	305 – 325	21	Helical; Potential
	Transmembrane	326 – 346	21	Helical; Potential
	Topological domain	347 – 357	11	Cytoplasmic Potential
	Transmembrane	358 – 378	21	Helical; Potential
	Topological domain	379 – 381	3	Lumenal Potential
	Transmembrane	382 – 402	21	Helical; Potential
	Topological domain	403 – 480	78	Cytoplasmic Potential
	Transmembrane	481 – 501	21	Helical; Potential
	Topological domain	502 – 529	28	Lumenal Potential
	Transmembrane	530 – 550	21	Helical; Potential
	Topological domain	551 – 632	82	Cytoplasmic Potential
	Repeat	89 – 118	30	ANK 1
	Repeat	123 – 152	30	ANK 2
	Repeat	156 – 185	30	ANK 3
	Repeat	189 – 219	31	ANK 4
	Repeat	224 – 253	30	ANK 5
	Zinc finger	437 – 487	51	DHHC-type
Sites
	Active site	467	1	S-palmitoyl cysteine intermediate By similarity
Amino acid modifications
	Modified residue	70	1	Phosphotyrosine Ref.8
Natural variations
	Alternative sequence	423 – 429	7	TIVELAE → VVMYPAN in isoform 2.		VSP_010026
	Alternative sequence	430 – 632	203	Missing in isoform 2.		VSP_010027
Experimental info
	Sequence conflict	288	1	N → K in BAC32912. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 13, 2004. Version 2. Checksum: 323EFD47790750DC	FASTA	632	72,621
	10 20 30 40 50 60 MQREEGFNTK MADGPDEYET ETGCVPLLHP EEIKPQSHYN HGYGEPLGRK THIDDYSTWD 70 80 90 100 110 120 IVKATQYGIY ERCRELVEAG YDVRQPDKEN VTLLHWAAIN NRIDLVKYYI SKGAIVDQLG 130 140 150 160 170 180 GDLNSTPLHW ATRQGHLSMV VQLMKYGADP SLIDGEGCSC IHLAAQFGHT SIVAYLIAKG 190 200 210 220 230 240 QDVDMMDQNG MTPLMWAAYR THSVDPTRLL LTFNVSVNLG DKYHKNTALH WAVLAGNTTV 250 260 270 280 290 300 ISLLLEAGGN VDAQNVKGES ALDLAKQRKN VWMINHLQEA RQAKGYDNPS FLRKLKADKE 310 320 330 340 350 360 FRQKVMLGTP FLVIWLVGFI ADLDIDSWLI KGLMYGGVWA TVQFLSKSFF DHSMHSALPL 370 380 390 400 410 420 GIYLATKFWM YVTWFFWFWN DLNFLFIHLP FLANSVALFY NFGKSWKSDP GIIKATEEQK 430 440 450 460 470 480 KKTIVELAET GSLDLSIFCS TCLIRKPVRS KHCGVCNRCI AKFDHHCPWV GNCVGAGNHR 490 500 510 520 530 540 YFMGYLFFLL FMICWMIYGC VSYWGLHCET TYTKDGFWTY ITQIATCSPW MFWMFLNSVF 550 560 570 580 590 600 HFLWVAVLLM CQLYQITCLG ITTNERMNAR RYKHFKVTTT SIESPFNHGC VRNIIDFFEF 610 620 630 RCCGLFRPVI VDWTRQYTIE YDQISGSGYQ LV
	Isoform 2 [UniParc]. Checksum: FE0E0E96934E4FC9	FASTA	429	48,784
	10 20 30 40 50 60 MQREEGFNTK MADGPDEYET ETGCVPLLHP EEIKPQSHYN HGYGEPLGRK THIDDYSTWD 70 80 90 100 110 120 IVKATQYGIY ERCRELVEAG YDVRQPDKEN VTLLHWAAIN NRIDLVKYYI SKGAIVDQLG 130 140 150 160 170 180 GDLNSTPLHW ATRQGHLSMV VQLMKYGADP SLIDGEGCSC IHLAAQFGHT SIVAYLIAKG 190 200 210 220 230 240 QDVDMMDQNG MTPLMWAAYR THSVDPTRLL LTFNVSVNLG DKYHKNTALH WAVLAGNTTV 250 260 270 280 290 300 ISLLLEAGGN VDAQNVKGES ALDLAKQRKN VWMINHLQEA RQAKGYDNPS FLRKLKADKE 310 320 330 340 350 360 FRQKVMLGTP FLVIWLVGFI ADLDIDSWLI KGLMYGGVWA TVQFLSKSFF DHSMHSALPL 370 380 390 400 410 420 GIYLATKFWM YVTWFFWFWN DLNFLFIHLP FLANSVALFY NFGKSWKSDP GIIKATEEQK KKVVMYPAN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H. DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 8-632 (ISOFORM 1). Strain: C57BL/6J. Tissue: Cerebellum and Hypothalamus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 9-418. Tissue: Eye.
[4]	"HIP14, a novel ankyrin domain-containing protein, links huntingtin to intracellular trafficking and endocytosis." Singaraja R.R., Hadano S., Metzler M., Givan S., Wellington C.L., Warby S., Yanai A., Gutekunst C.-A., Leavitt B.R., Yi H., Fichter K., Gan L., McGutcheon K., Chopra V., Michel J., Hersch S.M., Ikeda J.E., Hayden M.R. Hum. Mol. Genet. 11:2815-2828(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[5]	"Identification of PSD-95 palmitoylating enzymes." Fukata M., Fukata Y., Adesnik H., Nicoll R.A., Bredt D.S. Neuron 44:987-996(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Huntingtin interacting protein 14 is an oncogenic human protein: palmitoyl acyltransferase." Ducker C.E., Stettler E.M., French K.J., Upson J.J., Smith C.D. Oncogene 23:9230-9237(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Huntingtin-interacting proteins, HIP14 and HIP14L, mediate dual functions, palmitoyl acyltransferase and Mg2+ transport." Goytain A., Hines R.M., Quamme G.A. J. Biol. Chem. 283:33365-33374(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[8]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-70, MASS SPECTROMETRY. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK122406 mRNA. Translation: BAC65688.1. Different initiation. AK038670 mRNA. Translation: BAC30090.2. Different initiation. AK038681 mRNA. Translation: BAC30097.2. Different initiation. AK046891 mRNA. Translation: BAC32912.1. Different initiation. BC058772 mRNA. Translation: AAH58772.1. Sequence problems.
IPI	IPI00308479. IPI00410778.
RefSeq	NP_766142.2. NM_172554.2.
UniGene	Mm.339281.
3D structure databases
ProteinModelPortal	Q80TN5.
SMR	Q80TN5. Positions 21-281.
ModBase	Search...
PTM databases
PhosphoSite	Q80TN5.
Proteomic databases
PaxDb	Q80TN5.
PRIDE	Q80TN5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000041723; ENSMUSP00000043279; ENSMUSG00000035798.
GeneID	320150.
KEGG	mmu:320150.
UCSC	uc007gzu.2. mouse. uc007gzw.2. mouse.
Organism-specific databases
CTD	23390.
MGI	MGI:2445110. Zdhhc17.
Rouge	Search...
Phylogenomic databases
eggNOG	COG0666.
GeneTree	ENSGT00530000063074.
HOGENOM	HOG000290181.
HOVERGEN	HBG051907.
InParanoid	Q80TN5.
OMA	QAKGYDS.
OrthoDB	EOG44J2HH.
Gene expression databases
ArrayExpress	Q80TN5.
Bgee	Q80TN5.
CleanEx	MM_ZDHHC17.
Genevestigator	Q80TN5.
GermOnline	ENSMUSG00000035798. Mus musculus.
Family and domain databases
Gene3D	1.25.40.20. 1 hit.
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR001594. Znf_DHHC_palmitoyltrfase. [Graphical view]
Pfam	PF12796. Ank_2. 2 hits. PF01529. zf-DHHC. 1 hit. [Graphical view]
PRINTS	PR01415. ANKYRIN.
SMART	SM00248. ANK. 5 hits. [Graphical view]
SUPFAM	SSF48403. ANK. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 5 hits. PS50216. ZF_DHHC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	396133.
SOURCE	Search...

Entry information

Entry name

ZDH17_MOUSE

Accession

Primary (citable) accession number: Q80TN5
Secondary accession number(s): Q8BJ08, Q8BYQ2, Q8BYQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	April 13, 2004
Last modified:	May 1, 2013
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program