Q80TN4 (DJC16_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DnaJ homolog subfamily C member 16

Gene names

Name:	Dnajc16
Synonyms:	Kiaa0962

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	772 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Membrane; Single-pass type IV membrane protein Potential.
Sequence similarities	Contains 1 J domain. Contains 1 thioredoxin domain.
Sequence caution	The sequence AAH46424.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC65689.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Signal Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro protein folding Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	heat shock protein binding Inferred from electronic annotation. Source: InterPro unfolded protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 25

25

Potential

Chain

26 – 772

747

DnaJ homolog subfamily C member 16

PRO_0000236684

Regions

Topological domain

26 – 533

508

Cytoplasmic Potential

Transmembrane

534 – 554

21

Helical; Anchor for type IV membrane protein; Potential

Topological domain

555 – 772

218

Extracellular Potential

Domain

29 – 93

65

J

Domain

119 – 245

127

Thioredoxin

Amino acid modifications

Glycosylation

629

1

N-linked (GlcNAc...) Potential

Natural variations

Natural variant

478

1

G → A in strain: Czech II. Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

772

Helix Strand Turn

Details...

Beta strand

25 – 28

4

Helix

30 – 34

5

Helix

42 – 55

14

Turn

58 – 60

3

Helix

66 – 81

16

Helix

83 – 92

10

Sequences

	Sequence		Length	Mass (Da)
	Q80TN4 [UniParc]. Last modified May 30, 2006. Version 2. Checksum: 1B55F9494D6E0465	FASTA	772	89,136
	10 20 30 40 50 60 MELKRLGVSW RFLMVLVLIL QSLSALDFDP YRVLGVSRTA SQADIKKAYK KLAREWHPDK 70 80 90 100 110 120 NKDPGAEDRF IQISKAYEIL SNEEKRTNYD HYGDAGENQG YQKQQREHRF RHFHENFYFD 130 140 150 160 170 180 ESFFHFPFNA ERRDSGDEKY LLHFSHYVNE VLPESFKRPY LIKITSDWCF SCIHIEPVWK 190 200 210 220 230 240 EVVQELEGLG VGIGVVHAGY ERRLAHHLGA HSTPSILGVI SGKITFFHNA VVHENLRQFV 250 260 270 280 290 300 ESLLPGNLVE KVTNKNYVRF LSGWQQENKP HALLFGQTPA VPLMYKLTAF AYKDYVSFGY 310 320 330 340 350 360 VYVGLRGVEE MTRQYNVNLY TPTMLIFKEH INKPADVIQA RGLKKQVIED FIAQNKYLLA 370 380 390 400 410 420 SRLTSQRLFH ELCPVKRSHR QRKYCVVLLT AETNKVSKPF EAFLSFALAN TQDTVRFVHV 430 440 450 460 470 480 YSNRQQEFAS TLLPDMEAFQ GKSGVSILER RNTAGRVVFK TLEDPWTGSE SDKFVLLGYL 490 500 510 520 530 540 DQLRKDPAFL SSEAVLPDLT DELAPVFFLR WLYSVSDYLS DFWESLLHSN WREMMPLLSL 550 560 570 580 590 600 IFSALFILFG TVMVQAFSDS NEERESHPAD KEEVPEKAGK TEPSFTKESS SKIPKKGFVE 610 620 630 640 650 660 VTELTDVTYT SNLVRLRPGH MNVVLILSNS TKTSLLQKFA LEVYTFTGSS SLHFSFLTLD 670 680 690 700 710 720 KHREWLEYLL EFAQDAAPIP NQYDKHFMER DYTGYVLALN GHKKYFCLFK PLKTVDEETV 730 740 750 760 770 ASCDPDSSRG KPSCGLGPKP LKGKLSKLSL WMERLLEGSL QRFYIPSWPE LD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H. DNA Res. 10:35-48(2003) [PubMed: 12693553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NOD. Tissue: Embryo and Skin.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 312-772, VARIANT ALA-478. Strain: Czech II. Tissue: Mammary tumor.
[4]	"Solution structure of the J domain of the pseudo DnaJ protein, mouse hypothetical mKIAA0962." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 19-93.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK122407 mRNA. Translation: BAC65689.1. Different initiation.
AK028692 mRNA. Translation: BAC26068.1.
AK051048 mRNA. Translation: BAC34510.1.
AK154946 mRNA. Translation: BAE32943.1.
AK164023 mRNA. Translation: BAE37591.1.
BC046424 mRNA. Translation: AAH46424.1. Different initiation.

IPI

IPI00336247.

RefSeq

NP_758841.1. NM_172338.2.

UniGene

Mm.39102.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CUG	NMR	-	A	19-93	[»]

ProteinModelPortal

Q80TN4.

SMR

Q80TN4. Positions 19-250.

ModBase

Search...

Proteomic databases

PRIDE

Q80TN4.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000038014; ENSMUSP00000048714; ENSMUSG00000040697.

GeneID

214063.

KEGG

mmu:214063.

Organism-specific databases

CTD

23341.

MGI

MGI:2442146. Dnajc16.

Rouge

Search...

Phylogenomic databases

GeneTree

ENSGT00550000074587.

HOGENOM

HBG445587.

HOVERGEN

HBG055742.

InParanoid

Q80TN4.

OMA

KLTAFAY.

OrthoDB

EOG4SQWW3.

Gene expression databases

ArrayExpress

Q80TN4.

Bgee

Q80TN4.

CleanEx

MM_DNAJC16.

Genevestigator

Q80TN4.

GermOnline

ENSMUSG00000040697. Mus musculus.

Family and domain databases

InterPro

IPR001623. DnaJ_N.
IPR018253. Heat_shock_DnaJ_CS.
IPR003095. Hsp_DnaJ.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
[Graphical view]

Gene3D

G3DSA:1.10.287.110. DnaJ_N. 1 hit.
G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.

KO

K09536.

Pfam

PF00226. DnaJ. 1 hit.
PF00085. Thioredoxin. 1 hit.
[Graphical view]

PRINTS

PR00625. JDOMAIN.

SMART

SM00271. DnaJ. 1 hit.
[Graphical view]

SUPFAM

SSF46565. DnaJ_N. 1 hit.
SSF52833. Thiordxn-like_fd. 1 hit.

PROSITE

PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

374174.

SOURCE

Search...

Entry information

Entry name

DJC16_MOUSE

Accession

Primary (citable) accession number: Q80TN4
Secondary accession number(s): Q811G1, Q8BHI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	May 30, 2006
Last modified:	November 16, 2011
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program