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Protein

Calcium-dependent secretion activator 1

Gene

Cadps

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-binding protein involved in exocytosis of vesicles filled with neurotransmitters and neuropeptides. Probably acts upstream of fusion in the biogenesis or maintenance of mature secretory vesicles. Regulates catecholamine loading of DCVs. May specifically mediate the Ca2+-dependent exocytosis of large dense-core vesicles (DCVs) and other dense-core vesicles by acting as a PtdIns(4,5)P2-binding protein that acts at prefusion step following ATP-dependent priming and participates in DCVs-membrane fusion. However, it may also participate in small clear synaptic vesicles (SVs) exocytosis and it is unclear whether its function is related to Ca2+ triggering (By similarity).By similarity1 Publication

GO - Molecular functioni

  • lipid binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • protein kinase binding Source: ParkinsonsUK-UCL

GO - Biological processi

  • catecholamine secretion Source: MGI
  • dense core granule exocytosis Source: InterPro
  • protein transport Source: UniProtKB-KW
  • synaptic vesicle priming Source: MGI
  • vesicle organization Source: MGI
Complete GO annotation...

Keywords - Biological processi

Exocytosis, Protein transport, Transport

Keywords - Ligandi

Calcium, Lipid-binding, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent secretion activator 1
Alternative name(s):
Calcium-dependent activator protein for secretion 1
Short name:
CAPS-1
Gene namesi
Name:Cadps
Synonyms:Caps, Caps1, Kiaa1121
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1350922. Cadps.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cytoplasmic vesicle Source: MGI
  • cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  • presynapse Source: GOC
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Mice die within 30 minutes after birth but do not display obvious developmental or biochemical abnormalities. They show a strong reduction in the frequency of amperometrically detectable release events of transmitter-filled vesicles, while the total number of fusing vesicles, as judged by capacitance recordings or total internal reflection microscopy, remains unchanged.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000538651 – 1355Calcium-dependent secretion activator 1Add BLAST1355

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei34PhosphoserineCombined sources1
Modified residuei89PhosphoserineBy similarity1
Modified residuei96PhosphoserineBy similarity1
Modified residuei259PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ80TJ1.
PeptideAtlasiQ80TJ1.
PRIDEiQ80TJ1.

PTM databases

iPTMnetiQ80TJ1.
PhosphoSitePlusiQ80TJ1.
SwissPalmiQ80TJ1.

Expressioni

Tissue specificityi

Present in brain and adrenal glands (at protein level). Specifically expressed in neural and endocrine secretory tissues. Strongly expressed in almost all nerve cells of the brain, although it is absent from glial cells. Expressed in the cardiac atria, but not ventricles.3 Publications

Developmental stagei

During brain development, its expression is similar to that of synaptic markers. Expression is first detectable late in embryogenesis (E14) and increases to reach a plateau about 20 days after birth, when most synapses have been formed (at protein level).

Inductioni

In the heart, up-regulated by hypertrophic stimuli.1 Publication

Gene expression databases

BgeeiENSMUSG00000054423.
CleanExiMM_CADPS.
ExpressionAtlasiQ80TJ1. baseline and differential.
GenevisibleiQ80TJ1. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with the dopamine receptor DRD2 (By similarity). Interacts with RASL10B.By similarity1 Publication

GO - Molecular functioni

  • protein kinase binding Source: ParkinsonsUK-UCL

Protein-protein interaction databases

IntActiQ80TJ1. 4 interactors.
MINTiMINT-4109527.
STRINGi10090.ENSMUSP00000064706.

Structurei

3D structure databases

ProteinModelPortaliQ80TJ1.
SMRiQ80TJ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini398 – 479C2Add BLAST82
Domaini519 – 622PHPROSITE-ProRule annotationAdd BLAST104
Domaini933 – 1113MHD1PROSITE-ProRule annotationAdd BLAST181

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni792 – 1131Interaction with DRD2By similarityAdd BLAST340
Regioni1179 – 1355Mediates targeting and association with DCVsBy similarityAdd BLAST177

Domaini

The PH domain is essential for regulated exocytosis and binds phospholipids and plasma membrane. It however does not mediate binding to DCVs (By similarity).By similarity

Sequence similaritiesi

Contains 1 C2 domain.Curated
Contains 1 MHD1 (MUNC13 homology domain 1) domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3543. Eukaryota.
ENOG410Y0FD. LUCA.
GeneTreeiENSGT00590000083094.
HOGENOMiHOG000263607.
HOVERGENiHBG080678.
InParanoidiQ80TJ1.
KOiK19933.
OMAiFCLASQV.
OrthoDBiEOG091G00HB.
TreeFamiTF312963.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR033227. CAPS.
IPR010439. CAPS_dom.
IPR014770. Munc13_1.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PANTHERiPTHR12166. PTHR12166. 2 hits.
PfamiPF06292. DUF1041. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
SMARTiSM01145. DUF1041. 1 hit.
SM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS51258. MHD1. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80TJ1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLDPSSSEEE SDEILEEERG KDVLGSAASG ARLSPSRTSE GSAGSAGMGG
60 70 80 90 100
SGAGAGVGAG GGGGSGASSG GGAGGLQPSS RAGGGRPSSP SPSVVSEKEK
110 120 130 140 150
EELERLQKEE EERKKRLQLY VFVMRCIAYP FNAKQPTDMA RRQQKISKQQ
160 170 180 190 200
LQTVKDRFQA FLNGETQIVA DEAFMNAVQS YYEVFLKSDR VARMVQSGGC
210 220 230 240 250
SANDSREVFK KHIEKRVRSL PEIDGLSKET VLSSWMAKFD AIYRGEEDPR
260 270 280 290 300
KQQARMTASA ASELILSKEQ LYEMFQNILG IKKFEHQLLY NACQLDNPDE
310 320 330 340 350
QAAQIRRELD GRLQMADQIA RERKFPKFVS KEMENMYIEE LKSSVNLLMA
360 370 380 390 400
NLESMPVSKG GEFKLQKLKR SHNASIIDMG EESENQLSKS DVLLSFSLEV
410 420 430 440 450
VIMEVQGLKS LAPNRIVYCT MEVEGGEKLQ TDQAEASKPT WGTQGDFSTT
460 470 480 490 500
HALPAVKVKL FTESTGVLAL EDKELGRVIL HPTPNSPKQS EWHKMTVSKN
510 520 530 540 550
CPDQDLKIKL AVRMDKPQNM KHSGYLWTIG KNVWKRWKKR FFVLVQVSQY
560 570 580 590 600
TFAMCSYREK KAEPQELLQL DGYTVDYTDP QPGLEGGRAF FNAVKEGDTV
610 620 630 640 650
IFASDDEQDR ILWVQAMYRA TGQSHKPVPP TQVQKLNAKG GNVPQLDAPI
660 670 680 690 700
SQFSGLKDAD RAQKHGMDEF ISSNPCNFDH ASLFEMVQRL TLDHRLNDSY
710 720 730 740 750
SCLGWFSPGQ VFVLDEYCAR NGVRGCHRHL CYLRDLLERA ENGAMIDPTL
760 770 780 790 800
LHYSFAFCAS HVHGNRPDGI GTVTVEEKER FEEIKERLRV LLENQITHFR
810 820 830 840 850
YCFPFGRPEG ALKATLSLLE RVLMKDIVTP VPQEEVKTVI RKCLEQAALV
860 870 880 890 900
NYSRLSEYAK IEENQKDAEN VGRLITPAKK LEDTIRLAEL VIEVLQQNEE
910 920 930 940 950
HHAEPHVDKG EAFAWWSDLM VEHAETFLSL FAVDMDAALE VQPPDTWDSF
960 970 980 990 1000
PLFQLLNDFL RTDYNLCNGK FHKHLQDLFA PLVVRYVDLM ESSIAQSIHR
1010 1020 1030 1040 1050
GFERESWEPV KSLTSNLPNV NLPNVNLPKV PNLPVNIPLG IPQMPTFSAP
1060 1070 1080 1090 1100
SWMAAIYDAD NGSGTSEDLF WKLDALQTFI RDLHWPEEEF GKHLEQRLKL
1110 1120 1130 1140 1150
MASDMIESCV KRTRIAFEVK LQKTSRSTDF RVPQSICTMF NVMVDAKAQS
1160 1170 1180 1190 1200
TKLCSMEMGQ EHQYHSKIDE LIEETVKEMI TLLVAKFVTI LEGVLAKLSR
1210 1220 1230 1240 1250
YDEGTLFSSF LSFTVKAASK YVDVPKPGMD VADAYVTFVR HSQDVLRDKV
1260 1270 1280 1290 1300
NEEMYIERLF DQWYNSSMNI ICTWLTDRMD LQLHIYQLKT LIRMVKKTYR
1310 1320 1330 1340 1350
DFRLQGVLDS TLNSKTYETI RNRLTVEEAT ASVSEGGGLQ GISMKDSDEE

DEEDD
Length:1,355
Mass (Da):153,113
Last modified:July 27, 2011 - v3
Checksum:i59596BAD74C6AE56
GO
Isoform 2 (identifier: Q80TJ1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     654-658: SGLKD → Y
     863-869: ENQKDAE → GKKREMYEHPVFCLASQVMDLTIQ
     904-910: Missing.

Show »
Length:1,361
Mass (Da):154,004
Checksum:iC601C07CCC8D01F3
GO
Isoform 4 (identifier: Q80TJ1-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     905-910: PHVDKG → GK

Note: No experimental confirmation available.
Show »
Length:1,351
Mass (Da):152,664
Checksum:iE61DA7F964FE89FB
GO

Sequence cautioni

The sequence BAA13044 differs from that shown. The sequence differs from that shown due to a duplication of 90 bp after position 107.Curated
The sequence BAC65735 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19R → S in BAA13044 (Ref. 1) Curated1
Sequence conflicti19R → S in BAC65735 (PubMed:12693553).Curated1
Sequence conflicti86R → W in BAC65735 (PubMed:12693553).Curated1
Sequence conflicti908D → V in BAE36633 (PubMed:16141072).Curated1
Sequence conflicti1034P → S in BAE36633 (PubMed:16141072).Curated1
Sequence conflicti1252E → G in BAE36633 (PubMed:16141072).Curated1
Sequence conflicti1354D → E in BAA13044 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_016809654 – 658SGLKD → Y in isoform 2. 1 Publication5
Alternative sequenceiVSP_016811863 – 869ENQKDAE → GKKREMYEHPVFCLASQVMD LTIQ in isoform 2. 1 Publication7
Alternative sequenceiVSP_016813904 – 910Missing in isoform 2. 1 Publication7
Alternative sequenceiVSP_016814905 – 910PHVDKG → GK in isoform 4. 1 Publication6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86214 mRNA. Translation: BAA13044.1. Sequence problems.
AK122453 mRNA. Translation: BAC65735.1. Different initiation.
AC116395 Genomic DNA. No translation available.
AC154250 Genomic DNA. No translation available.
AC154592 Genomic DNA. No translation available.
AC156025 Genomic DNA. No translation available.
AK161919 mRNA. Translation: BAE36633.1.
BC057065 mRNA. Translation: AAH57065.1.
CCDSiCCDS36806.1. [Q80TJ1-2]
CCDS36807.1. [Q80TJ1-1]
RefSeqiNP_001036082.1. NM_001042617.1. [Q80TJ1-1]
NP_036191.2. NM_012061.3. [Q80TJ1-2]
XP_006518098.1. XM_006518035.3. [Q80TJ1-4]
UniGeneiMm.260881.

Genome annotation databases

EnsembliENSMUST00000067491; ENSMUSP00000064706; ENSMUSG00000054423. [Q80TJ1-2]
ENSMUST00000112658; ENSMUSP00000108277; ENSMUSG00000054423. [Q80TJ1-1]
GeneIDi27062.
KEGGimmu:27062.
UCSCiuc007sfu.1. mouse. [Q80TJ1-1]
uc007sfv.1. mouse. [Q80TJ1-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86214 mRNA. Translation: BAA13044.1. Sequence problems.
AK122453 mRNA. Translation: BAC65735.1. Different initiation.
AC116395 Genomic DNA. No translation available.
AC154250 Genomic DNA. No translation available.
AC154592 Genomic DNA. No translation available.
AC156025 Genomic DNA. No translation available.
AK161919 mRNA. Translation: BAE36633.1.
BC057065 mRNA. Translation: AAH57065.1.
CCDSiCCDS36806.1. [Q80TJ1-2]
CCDS36807.1. [Q80TJ1-1]
RefSeqiNP_001036082.1. NM_001042617.1. [Q80TJ1-1]
NP_036191.2. NM_012061.3. [Q80TJ1-2]
XP_006518098.1. XM_006518035.3. [Q80TJ1-4]
UniGeneiMm.260881.

3D structure databases

ProteinModelPortaliQ80TJ1.
SMRiQ80TJ1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ80TJ1. 4 interactors.
MINTiMINT-4109527.
STRINGi10090.ENSMUSP00000064706.

PTM databases

iPTMnetiQ80TJ1.
PhosphoSitePlusiQ80TJ1.
SwissPalmiQ80TJ1.

Proteomic databases

PaxDbiQ80TJ1.
PeptideAtlasiQ80TJ1.
PRIDEiQ80TJ1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000067491; ENSMUSP00000064706; ENSMUSG00000054423. [Q80TJ1-2]
ENSMUST00000112658; ENSMUSP00000108277; ENSMUSG00000054423. [Q80TJ1-1]
GeneIDi27062.
KEGGimmu:27062.
UCSCiuc007sfu.1. mouse. [Q80TJ1-1]
uc007sfv.1. mouse. [Q80TJ1-2]

Organism-specific databases

CTDi8618.
MGIiMGI:1350922. Cadps.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG3543. Eukaryota.
ENOG410Y0FD. LUCA.
GeneTreeiENSGT00590000083094.
HOGENOMiHOG000263607.
HOVERGENiHBG080678.
InParanoidiQ80TJ1.
KOiK19933.
OMAiFCLASQV.
OrthoDBiEOG091G00HB.
TreeFamiTF312963.

Miscellaneous databases

ChiTaRSiCadps. mouse.
PROiQ80TJ1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000054423.
CleanExiMM_CADPS.
ExpressionAtlasiQ80TJ1. baseline and differential.
GenevisibleiQ80TJ1. MM.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR033227. CAPS.
IPR010439. CAPS_dom.
IPR014770. Munc13_1.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PANTHERiPTHR12166. PTHR12166. 2 hits.
PfamiPF06292. DUF1041. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
SMARTiSM01145. DUF1041. 1 hit.
SM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS51258. MHD1. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAPS1_MOUSE
AccessioniPrimary (citable) accession number: Q80TJ1
Secondary accession number(s): E9QN90
, Q3TSP2, Q61374, Q6AXB4, Q6PGF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 116 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.