Q80TG9 (LRFN2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leucine-rich repeat and fibronectin type-III domain-containing protein 2

Gene names

Name:	Lrfn2
Synonyms:	Kiaa1246, Salm1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	788 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Promotes neurite outgrowth in hippocampal neurons. Enhances the cell surface expression of 2 NMDA receptor subunits GRIN1 and GRIN2A By similarity. May play a role in redistributing DLG4 to the cell periphery. Ref.5
Subunit structure	Forms heteromeric complexes with LRFN1, LRFN3, LRFN4 and LRFN5. Can form homomeric complexes, but not across cell junctions By similarity. Interacts with DLG4. Directly interacts with DLG1, DLG2 and DLG3 By similarity. Directly interacts with 2 NMDA receptor subunits GRIN1 and GRIN2A By similarity. Ref.5
Subcellular location	Membrane; Single-pass type I membrane protein. Cell junction › synapse. Cell junction › synapse › postsynaptic cell membrane By similarity Ref.5.
Tissue specificity	Predominantly expressed in the brain, with a weak, but broad expression in the cerebral cortex and diencephalic nuclei. Strongly expressed in both the pyramidal layer and the dentate gyrus of the hippocampus. Also detected in other parts of the central nervous system, including the olfactory bulb, pons, cerebellum, and medulla oblongata, as well as in the peripheral nervous system, such as the ganglia of cranial nerves and the dorsal root ganglion during gestation. Ref.5
Developmental stage	Expression starts around 11.5-12.5 dpc. At 11.5 dpc, detected in the outer layer of the telencephalic vesicles. This pattern of expression continues until 17.5 dpc with expression in the cortical plate, but not in the inner layer of the cerebral cortex, including subplate, ventricular zone, and subventricular zone. As also detected in the hippocampus, amygdala and widely in diencephalic nuclei. Ref.5
Domain	The PDZ-binding motif is required for cell surface expression, neurite outgrowth promotion and interaction with DLG1, DLG3 and DLG4 By similarity.
Post-translational modification	Glycosylated. Ref.5
Sequence similarities	Belongs to the LRFN family. Contains 1 fibronectin type-III domain. Contains 1 Ig-like (immunoglobulin-like) domain. Contains 7 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain.
Sequence caution	The sequence BAC65758.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Immunoglobulin domain Leucine-rich repeat Repeat Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Dlg1	Q811D0	3	EBI-877092,EBI-514290
Dlg4	Q62108	2	EBI-877092,EBI-300895
Grin1	P35439	2	EBI-877092,EBI-877897	From a different organism.
Grin1	P35439-1	2	EBI-877092,EBI-877923	From a different organism.
Grin1	P35439-4	2	EBI-877092,EBI-877935	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 788	768	Leucine-rich repeat and fibronectin type-III domain-containing protein 2		PRO_0000014840
Regions
	Topological domain	21 – 534	514	Extracellular Potential
	Transmembrane	535 – 555	21	Helical; Potential
	Topological domain	556 – 788	233	Cytoplasmic Potential
	Domain	21 – 52	32	LRRNT
	Repeat	53 – 74	22	LRR 1
	Repeat	77 – 98	22	LRR 2
	Repeat	101 – 122	22	LRR 3
	Repeat	125 – 146	22	LRR 4
	Repeat	150 – 171	22	LRR 5
	Repeat	174 – 195	22	LRR 6
	Repeat	198 – 219	22	LRR 7
	Domain	242 – 288	47	LRRCT
	Domain	289 – 375	87	Ig-like
	Domain	419 – 510	92	Fibronectin type-III
	Motif	785 – 788	4	PDZ-binding
Amino acid modifications
	Glycosylation	29	1	N-linked (GlcNAc...) Potential
	Glycosylation	332	1	N-linked (GlcNAc...) Potential
	Glycosylation	341	1	N-linked (GlcNAc...) Potential
	Glycosylation	384	1	N-linked (GlcNAc...) Potential
	Disulfide bond	310 ↔ 359		By similarity
Experimental info
	Mutagenesis	787 – 788	2	Missing: Loss of DLG4-binding. Ref.5
	Sequence conflict	42	1	G → R in BAB30828. Ref.1
	Sequence conflict	530	1	L → K in BAB30828. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q80TG9 [UniParc]. Last modified March 29, 2005. Version 2. Checksum: 65B70CA475E99D6B	FASTA	788	84,963
	10 20 30 40 50 60 METLLGGLLA FGMAFAVVDA CPKYCVCQNL SESLGTLCPS KGLLFVPPDI DRRTVELRLG 70 80 90 100 110 120 GNFIIHIGRQ DFANMTGLVD LTLSRNTISH IQPFSFLDLE SLRSLHLDSN RLPSLGEDTL 130 140 150 160 170 180 RGLVNLQHLI VNNNQLGGIA DDAFEDFLLT LEDLDLSYNN LHGLPWDSVR RMVNLHQLSL 190 200 210 220 230 240 DHNLLDHIAE GTFADLQKLA RLDLTSNRLQ KLPPDPIFAR SQASLLTATP FAPPLSFSFG 250 260 270 280 290 300 GNPLHCNCEL LWLRRLERDD DLETCGSPGS LKGRYFWHIR EEEFVCEPPL ITQHTHKLLV 310 320 330 340 350 360 LEGQAATLKC KAIGDPSPLI HWVAPDDRLV GNSSRTAVYD NGTLDILITT SQDSGPFTCI 370 380 390 400 410 420 AANAAGEATA TVEVSIVQLP HLSNSTSRMA PPKSRLSDIT GSSKTSRGGG GSGAGEPPKS 430 440 450 460 470 480 TPERAVLVSD VTTTSALVKW SVSKSAPRVK MYQLQYNCSD DEVLIYRMIP ASNKAFVVNN 490 500 510 520 530 540 LVSGTGYDLC VLAMWDDTAT TLTATNIVGC AQFFTKADYP QCQSMHSQIL GGTMILVIGG 550 560 570 580 590 600 IIVATLLVFI VILMVRYKVC NHDTPGKMAA ATVSNVYSQT NGSQPPPLGG IPVGQLPQAP 610 620 630 640 650 660 PKVVVRNELM DFSTSLARAC DSSSSSSLGS GEAAGLGRGP WRLPPPAPRP KPSLDRLMGA 670 680 690 700 710 720 FASLDLKSQR KEELLDSRTP AGRGAGTSSR GHHSDREPLL GPPATRARSL LPLPLEGKAK 730 740 750 760 770 780 RSHSFDMGDF AAAAAAVPGG YSPPRRVSNI WTKRSLSVNG MLLPFEESDL VGARGTFGSS EWVMESTV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Embryo and Retina.
[2]	"Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H. DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Comparative analysis of structure, expression and PSD95-binding capacity of Lrfn, a novel family of neuronal transmembrane proteins." Morimura N., Inoue T., Katayama K., Aruga J. Gene 380:72-83(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH DLG4, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, GLYCOSYLATION, SUBCELLULAR LOCATION, TOPOLOGY, MUTAGENESIS OF 787-THR-VAL-788.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK017594 mRNA. Translation: BAB30828.1. AK044375 mRNA. Translation: BAC31891.1. AK122476 mRNA. Translation: BAC65758.1. Different initiation. CH466559 Genomic DNA. Translation: EDL23629.1. BC139418 mRNA. Translation: AAI39419.1.
IPI	IPI00330152.
RefSeq	NP_081728.2. NM_027452.3.
UniGene	Mm.133607.
3D structure databases
ProteinModelPortal	Q80TG9.
SMR	Q80TG9. Positions 20-461.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q80TG9. 6 interactions.
Protein family/group databases
MEROPS	I43.001.
PTM databases
PhosphoSite	Q80TG9.
Proteomic databases
PaxDb	Q80TG9.
PRIDE	Q80TG9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000046254; ENSMUSP00000047573; ENSMUSG00000040490.
GeneID	70530.
KEGG	mmu:70530.
UCSC	uc008cyd.2. mouse.
Organism-specific databases
CTD	57497.
MGI	MGI:1917780. Lrfn2.
Rouge	Search...
Phylogenomic databases
eggNOG	NOG311542.
GeneTree	ENSGT00660000095307.
HOGENOM	HOG000237343.
HOVERGEN	HBG052352.
InParanoid	Q80TG9.
KO	K16355.
OMA	HWVAPDD.
OrthoDB	EOG469QT6.
Gene expression databases
Bgee	Q80TG9.
Genevestigator	Q80TG9.
GermOnline	ENSMUSG00000040490. Mus musculus.
Family and domain databases
Gene3D	2.60.40.10. 2 hits.
InterPro	IPR000483. Cys-rich_flank_reg_C. IPR003961. Fibronectin_type3. IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR013098. Ig_I-set. IPR003598. Ig_sub2. IPR001611. Leu-rich_rpt. IPR003591. Leu-rich_rpt_typical-subtyp. IPR000372. LRR-contain_N. [Graphical view]
Pfam	PF00041. fn3. 1 hit. PF07679. I-set. 1 hit. [Graphical view]
SMART	SM00060. FN3. 1 hit. SM00408. IGc2. 1 hit. SM00369. LRR_TYP. 3 hits. SM00082. LRRCT. 1 hit. SM00013. LRRNT. 1 hit. [Graphical view]
SUPFAM	SSF49265. FN_III-like. 1 hit.
PROSITE	PS50853. FN3. 1 hit. PS50835. IG_LIKE. 1 hit. PS51450. LRR. 6 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	331801.
SOURCE	Search...

Entry information

Entry name

LRFN2_MOUSE

Accession

Primary (citable) accession number: Q80TG9
Secondary accession number(s): B9EIF8, Q9CYK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	March 29, 2005
Last modified:	May 1, 2013
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program