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Protein

Gamma-aminobutyric acid type B receptor subunit 2

Gene

Gabbr2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2. Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-MMU-1296041. Activation of G protein gated Potassium channels.
R-MMU-418594. G alpha (i) signalling events.
R-MMU-420499. Class C/3 (Metabotropic glutamate/pheromone receptors).
R-MMU-977444. GABA B receptor activation.
R-MMU-997272. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid type B receptor subunit 2
Short name:
GABA-B receptor 2
Short name:
GABA-B-R2
Short name:
GABA-BR2
Short name:
GABABR2
Short name:
Gb2
Alternative name(s):
G-protein coupled receptor 51
Gene namesi
Name:Gabbr2
Synonyms:Gm425, Gpr51
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2386030. Gabbr2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini41 – 482442ExtracellularSequence analysisAdd
BLAST
Transmembranei483 – 50321Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini504 – 52118CytoplasmicSequence analysisAdd
BLAST
Transmembranei522 – 54221Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini543 – 5508ExtracellularSequence analysis
Transmembranei551 – 57121Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini572 – 59625CytoplasmicSequence analysisAdd
BLAST
Transmembranei597 – 61721Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini618 – 65336ExtracellularSequence analysisAdd
BLAST
Transmembranei654 – 67421Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini675 – 69016CytoplasmicSequence analysisAdd
BLAST
Transmembranei691 – 71121Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini712 – 7198ExtracellularSequence analysis
Transmembranei720 – 74021Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini741 – 940200CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cytoplasm Source: MGI
  • G-protein coupled receptor heterodimeric complex Source: UniProtKB
  • integral component of plasma membrane Source: UniProtKB
  • neuron projection Source: MGI
  • postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4040Sequence analysisAdd
BLAST
Chaini41 – 940900Gamma-aminobutyric acid type B receptor subunit 2PRO_0000306250Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi89 – 891N-linked (GlcNAc...)Sequence analysis
Disulfide bondi107 ↔ 134By similarity
Disulfide bondi236 ↔ 265By similarity
Disulfide bondi264 ↔ 301By similarity
Glycosylationi297 – 2971N-linked (GlcNAc...)Sequence analysis
Glycosylationi388 – 3881N-linked (GlcNAc...)Sequence analysis
Glycosylationi403 – 4031N-linked (GlcNAc...)Sequence analysis
Glycosylationi452 – 4521N-linked (GlcNAc...)Sequence analysis
Modified residuei775 – 7751PhosphoserineCombined sources
Modified residuei778 – 7781PhosphoserineCombined sources
Modified residuei818 – 8181PhosphothreonineCombined sources
Modified residuei883 – 8831PhosphoserineCombined sources
Modified residuei892 – 8921PhosphoserineCombined sources
Modified residuei912 – 9121PhosphoserineCombined sources
Modified residuei915 – 9151PhosphoserineCombined sources
Modified residuei919 – 9191PhosphoserineCombined sources
Modified residuei923 – 9231PhosphoserineCombined sources

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ80T41.
PaxDbiQ80T41.
PRIDEiQ80T41.

PTM databases

iPTMnetiQ80T41.
PhosphoSiteiQ80T41.

Expressioni

Gene expression databases

BgeeiQ80T41.
CleanExiMM_GABBR2.

Interactioni

Subunit structurei

Heterodimer of GABBR1 and GABBR2. Homodimers may form, but are inactive. Interacts with ATF4 via its C-terminal region (By similarity).By similarity

Protein-protein interaction databases

BioGridi232404. 2 interactions.
IntActiQ80T41. 1 interaction.
STRINGi10090.ENSMUSP00000103378.

Structurei

3D structure databases

ProteinModelPortaliQ80T41.
SMRiQ80T41. Positions 51-463, 483-751, 778-816.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili781 – 81838Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi4 – 5451Pro-richAdd
BLAST

Domaini

Alpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABBR1.By similarity

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1055. Eukaryota.
ENOG410XNN1. LUCA.
GeneTreeiENSGT00530000063129.
HOGENOMiHOG000112688.
HOVERGENiHBG080355.
InParanoidiQ80T41.
KOiK04615.
OMAiRFQGVEP.
OrthoDBiEOG7D2FCV.
PhylomeDBiQ80T41.
TreeFamiTF313965.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR002455. GPCR3_GABA-B.
IPR000337. GPCR_3.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR002457. GPCR_3_GABA_rcpt_B2.
IPR028082. Peripla_BP_I.
[Graphical view]
PANTHERiPTHR10519. PTHR10519. 1 hit.
PTHR10519:SF40. PTHR10519:SF40. 1 hit.
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
[Graphical view]
PRINTSiPR01178. GABAB2RECPTR.
PR00248. GPCRMGR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q80T41-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASPPSSGQP RPPPPPPPPA RLLLPLLLSL LLSLAPGAWG WARGAPRPPP
60 70 80 90 100
SSPPLSIMGL MPLTKEVAKG SIGRGVLPAV ELAIEQIRNE SLLRPYFLDL
110 120 130 140 150
RLYDTECDNA KGLKAFYDAI KYGPNHLMVF GGVCPSVTSI IAESLQGWNL
160 170 180 190 200
VQLSFAATTP VLADKKKYPY FFRTVPSDNA VNPAILKLLK HFRWRRVGTL
210 220 230 240 250
TQDVQRFSEV RNDLTGVLYG EDIEISDTES FSNDPCTSVK KLKGNDVRII
260 270 280 290 300
LGQFDQNMAA KVFCCAFEES MFGSKYQWII PGWYEPAWWE QVHVEANSSR
310 320 330 340 350
CLRRSLLAAM EGYIGVDFEP LSSKQIKTIS GKTPQQYERE YNSKRSGVGP
360 370 380 390 400
SKFHGYAYDG IWVIAKTLQR AMETLHASSR HQRIQDFNYT DHTLGRIILN
410 420 430 440 450
AMNETNFFGV TGQVVFRNGE RMGTIKFTQF QDSREVKVGE YNAVADTLEI
460 470 480 490 500
INDTIRFQGS EPPKDKTIIL EQLRKISLPL YSILSALTIL GMIMASAFLF
510 520 530 540 550
FNIKNRNQKL IKMSSPYMNN LIILGGMLSY ASIFLFGLDG SFVSEKTFET
560 570 580 590 600
LCTVRTWILT VGYTTAFGAM FAKTWRVHAI FKNVKMKKKI IKDQKLLVIV
610 620 630 640 650
GGMLLIDLCI LICWQAVDPL RRTVERYSME PDPAGRDISI RPLLEHCENT
660 670 680 690 700
HMTIWLGIVY AYKGLLMLFG CFLAWETRNV SIPALNDSKY IGMSVYNVGI
710 720 730 740 750
MCIIGAAVSF LTRDQPNVQF CIVALVIIFC STITLCLVFV PKLITLRTNP
760 770 780 790 800
DAATQNRRFQ FTQNQKKEDS KTSTSVTSVN QASTSRLEGL QSENHRLRMK
810 820 830 840 850
ITELDKDLEE VTMQLQDTPE KTTYIKQNHY QELNDILSLG NFTESTDGGK
860 870 880 890 900
AILKNHLDQN PQLQWNTTEP SRTCKDPIED INSPEHIQRR LSLQLPILHH
910 920 930 940
AYLPSIGGVD ASCVSPCVSP TASPRHRHVP PSFRVMVSGL
Length:940
Mass (Da):105,666
Last modified:October 2, 2007 - v2
Checksum:iB364DC0A54EE9206
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL772399
, AL831741, AL844224, BX005247, BX571848 Genomic DNA. Translation: CAM17855.1.
AL831741
, AL772399, AL844224, BX005247, BX571848 Genomic DNA. Translation: CAM21407.1.
BX005247
, AL772399, AL831741, AL844224, BX571848 Genomic DNA. Translation: CAM22528.1.
BX571848
, AL772399, AL831741, AL844224, BX005247 Genomic DNA. Translation: CAM27679.1.
AL844224
, AL772399, AL831741, BX005247, BX571848 Genomic DNA. Translation: CAM27833.1.
AY255605 mRNA. Translation: AAO85117.1.
CCDSiCCDS38758.1.
RefSeqiNP_001074610.1. NM_001081141.1.
UniGeneiMm.101909.

Genome annotation databases

EnsembliENSMUST00000107749; ENSMUSP00000103378; ENSMUSG00000039809.
GeneIDi242425.
KEGGimmu:242425.
UCSCiuc008sug.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL772399
, AL831741, AL844224, BX005247, BX571848 Genomic DNA. Translation: CAM17855.1.
AL831741
, AL772399, AL844224, BX005247, BX571848 Genomic DNA. Translation: CAM21407.1.
BX005247
, AL772399, AL831741, AL844224, BX571848 Genomic DNA. Translation: CAM22528.1.
BX571848
, AL772399, AL831741, AL844224, BX005247 Genomic DNA. Translation: CAM27679.1.
AL844224
, AL772399, AL831741, BX005247, BX571848 Genomic DNA. Translation: CAM27833.1.
AY255605 mRNA. Translation: AAO85117.1.
CCDSiCCDS38758.1.
RefSeqiNP_001074610.1. NM_001081141.1.
UniGeneiMm.101909.

3D structure databases

ProteinModelPortaliQ80T41.
SMRiQ80T41. Positions 51-463, 483-751, 778-816.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi232404. 2 interactions.
IntActiQ80T41. 1 interaction.
STRINGi10090.ENSMUSP00000103378.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiQ80T41.
PhosphoSiteiQ80T41.

Proteomic databases

MaxQBiQ80T41.
PaxDbiQ80T41.
PRIDEiQ80T41.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000107749; ENSMUSP00000103378; ENSMUSG00000039809.
GeneIDi242425.
KEGGimmu:242425.
UCSCiuc008sug.1. mouse.

Organism-specific databases

CTDi9568.
MGIiMGI:2386030. Gabbr2.

Phylogenomic databases

eggNOGiKOG1055. Eukaryota.
ENOG410XNN1. LUCA.
GeneTreeiENSGT00530000063129.
HOGENOMiHOG000112688.
HOVERGENiHBG080355.
InParanoidiQ80T41.
KOiK04615.
OMAiRFQGVEP.
OrthoDBiEOG7D2FCV.
PhylomeDBiQ80T41.
TreeFamiTF313965.

Enzyme and pathway databases

ReactomeiR-MMU-1296041. Activation of G protein gated Potassium channels.
R-MMU-418594. G alpha (i) signalling events.
R-MMU-420499. Class C/3 (Metabotropic glutamate/pheromone receptors).
R-MMU-977444. GABA B receptor activation.
R-MMU-997272. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

Miscellaneous databases

NextBioi385343.
PROiQ80T41.
SOURCEiSearch...

Gene expression databases

BgeeiQ80T41.
CleanExiMM_GABBR2.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR002455. GPCR3_GABA-B.
IPR000337. GPCR_3.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR002457. GPCR_3_GABA_rcpt_B2.
IPR028082. Peripla_BP_I.
[Graphical view]
PANTHERiPTHR10519. PTHR10519. 1 hit.
PTHR10519:SF40. PTHR10519:SF40. 1 hit.
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
[Graphical view]
PRINTSiPR01178. GABAB2RECPTR.
PR00248. GPCRMGR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 869-940.
  3. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-883, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-775; SER-778; THR-818; SER-883; SER-892; SER-912; SER-915; SER-919 AND SER-923, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiGABR2_MOUSE
AccessioniPrimary (citable) accession number: Q80T41
Secondary accession number(s): A2AL05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: January 20, 2016
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.