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Protein

E3 ubiquitin-protein ligase MIB1

Gene

Mib1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Involved in ubiquitination of centriolar satellite CEP131, CEP290 and PCM1 proteins and hence inhibits primary cilium formation in proliferating cells. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation (By similarity). Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.By similarity1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri79 – 126ZZ-typePROSITE-ProRule annotationAdd BLAST48
Zinc fingeri819 – 854RING-type 1PROSITE-ProRule annotationAdd BLAST36
Zinc fingeri866 – 901RING-type 2PROSITE-ProRule annotationAdd BLAST36
Zinc fingeri963 – 996RING-type 3PROSITE-ProRule annotationAdd BLAST34

GO - Molecular functioni

GO - Biological processi

  • blood vessel development Source: MGI
  • heart development Source: MGI
  • heart looping Source: MGI
  • in utero embryonic development Source: MGI
  • negative regulation of neuron differentiation Source: MGI
  • neural tube formation Source: MGI
  • Notch signaling pathway Source: MGI
  • positive regulation of endocytosis Source: MGI
  • somitogenesis Source: MGI

Keywordsi

Molecular functionTransferase
Biological processNotch signaling pathway, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase MIB1 (EC:2.3.2.27)
Alternative name(s):
DAPK-interacting protein 1
Short name:
DIP-1
Mind bomb homolog 1
RING-type E3 ubiquitin transferase MIB1Curated
Gene namesi
Name:Mib1
Synonyms:Dip1, Kiaa1323, Mib
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:2443157. Mib1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000559441 – 1006E3 ubiquitin-protein ligase MIB1Add BLAST1006

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei408PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated; this modification is inhibited in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock (By similarity). Ubiquitinated; possibly via autoubiquitination.By similarity2 Publications

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ80SY4.
MaxQBiQ80SY4.
PaxDbiQ80SY4.
PRIDEiQ80SY4.

PTM databases

iPTMnetiQ80SY4.
PhosphoSitePlusiQ80SY4.

Expressioni

Tissue specificityi

Detected in all tissues tested. Present in embryo, embryonic stem cells, bladder, skeletal muscle, bladder, uterus, testis, stomach, colon, ileum, trachea, lung, aorta, kidney, spleen, liver and vas deferens (at protein level). Highly expressed in testis.2 Publications

Developmental stagei

Highly expressed both in embryos and adult tissues. In E9.5 and E10.5 embryos, it is expressed in the tail bud, limb buds and somites. Expressed in the same pattern than MIB2 in the skin and intestine at postnatal day 1 (P1) and in the hair follicle in the skin in the adult.1 Publication

Gene expression databases

BgeeiENSMUSG00000024294.
CleanExiMM_MIB1.
ExpressionAtlasiQ80SY4. baseline and differential.
GenevisibleiQ80SY4. MM.

Interactioni

Subunit structurei

Interacts with CEP131 and PCM1.By similarity

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi230365. 43 interactors.
IntActiQ80SY4. 32 interactors.
MINTiMINT-1550820.
STRINGi10090.ENSMUSP00000054428.

Structurei

3D structure databases

ProteinModelPortaliQ80SY4.
SMRiQ80SY4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 74MIB/HERC2 1PROSITE-ProRule annotationAdd BLAST69
Domaini143 – 221MIB/HERC2 2PROSITE-ProRule annotationAdd BLAST79
Repeati430 – 460ANK 1Add BLAST31
Repeati463 – 492ANK 2Add BLAST30
Repeati496 – 525ANK 3Add BLAST30
Repeati529 – 558ANK 4Add BLAST30
Repeati562 – 591ANK 5Add BLAST30
Repeati595 – 627ANK 6Add BLAST33
Repeati631 – 661ANK 7Add BLAST31
Repeati665 – 694ANK 8Add BLAST30
Repeati698 – 729ANK 9Add BLAST32

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili935 – 962Sequence analysisAdd BLAST28

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri79 – 126ZZ-typePROSITE-ProRule annotationAdd BLAST48
Zinc fingeri819 – 854RING-type 1PROSITE-ProRule annotationAdd BLAST36
Zinc fingeri866 – 901RING-type 2PROSITE-ProRule annotationAdd BLAST36
Zinc fingeri963 – 996RING-type 3PROSITE-ProRule annotationAdd BLAST34

Keywords - Domaini

ANK repeat, Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
KOG4582. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00900000141005.
HOVERGENiHBG068386.
InParanoidiQ80SY4.
KOiK10645.
OMAiCYNERKT.
OrthoDBiEOG091G00Z0.
PhylomeDBiQ80SY4.
TreeFamiTF324147.

Family and domain databases

CDDicd00204. ANK. 2 hits.
Gene3Di1.25.40.20. 3 hits.
3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR036770. Ankyrin_rpt-contain_sf.
IPR010606. Mib_Herc2.
IPR037252. Mib_Herc2_sf.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR000433. Znf_ZZ.
PfamiView protein in Pfam
PF12796. Ank_2. 3 hits.
PF06701. MIB_HERC2. 2 hits.
PF00569. ZZ. 1 hit.
PRINTSiPR01415. ANKYRIN.
SMARTiView protein in SMART
SM00248. ANK. 9 hits.
SM00184. RING. 3 hits.
SM00291. ZnF_ZZ. 1 hit.
SUPFAMiSSF159034. SSF159034. 2 hits.
SSF48403. SSF48403. 1 hit.
PROSITEiView protein in PROSITE
PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 6 hits.
PS51416. MIB_HERC2. 2 hits.
PS50089. ZF_RING_2. 3 hits.
PS01357. ZF_ZZ_1. 1 hit.
PS50135. ZF_ZZ_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q80SY4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNSRNNRVM VEGVGARVVR GPDWKWGKQD GGEGHVGTVR SFESPEEVVV
60 70 80 90 100
VWDNGTAANY RCSGAYDLRI LDSAPTGIKH DGTMCDTCRQ QPIIGIRWKC
110 120 130 140 150
AECTNYDLCT VCYHGDKHHL RHRFYRITTP GSERVLLESR RKSKKITARG
160 170 180 190 200
IFAGARVVRG VDWQWEDQDG GNGRRGKVTE IQDWSASSPH SAAYVLWDNG
210 220 230 240 250
AKNLYRVGFE GMSDLKCVQD AKGGSFYRDH CPVLGEQNGN RNPGGLQIGD
260 270 280 290 300
LVNIDLDLEI VQSLQHGHGG WTDGMFETLT TTGTVCGIDE DHDIVVQYPS
310 320 330 340 350
GNRWTFNPAV LTKANIVRSG DAAQGAEGGT SQFQVGDLVQ VCYDLERIKL
360 370 380 390 400
LQRGHGEWAE AMLPTLGKVG RVQQIYSDSD LKVEVCGTSW TYNPAAVSKV
410 420 430 440 450
APAGSAISNA SGERLSQLLK KLFETQESGD LNEELVKAAA NGDVAKVEDL
460 470 480 490 500
LKRPDVDVNG QCAGHTAMQA ASQNGHVDIL KLLLKQNVDV EAEDKDGDRA
510 520 530 540 550
VHHAAFGDEG AVIEVLHRGS ADLNARNKRR QTPLHIAVNK GHLQVVKTLL
560 570 580 590 600
DFGCHPSLQD SEGDTPLHDA ISKKRDDILA VLLEAGADVT ITNNNGFNAL
610 620 630 640 650
HHAALRGNPS AMRVLLSKLP RPWIVDEKKD DGYTALHLAA LNNHVEVAEL
660 670 680 690 700
LVHQGNANLD IQNVNQQTAL HLAVERQHTQ IVRLLVRAGA KLDIQDKDGD
710 720 730 740 750
TPLHEALRHH TLSQLRQLQD MQDVGKVDAA WEPSKNTLIM GLGTQGAEKK
760 770 780 790 800
SAASIACFLA ANGADLSIRN KKGQSPLDLC PDPSLCKALA KCHKEKVSGQ
810 820 830 840 850
VGSRSPSMIS NDSETLEECM VCSDMKRDTL FGPCGHIATC SLCSPRVKKC
860 870 880 890 900
LICKEQVQSR TKIEECVVCS DKKAAVLFQP CGHMCACENC ASLMKKCVQC
910 920 930 940 950
RAVVERRVPF ITCCGGKSSE DPSDEISSGN IPVLQKDKDN TNVNADVQKL
960 970 980 990 1000
QQQLQDIKEQ TMCPVCLDRL KNMIFLCGHG TCQLCGDRMS ECPICRKAIE

RRILLY
Length:1,006
Mass (Da):110,088
Last modified:June 1, 2003 - v1
Checksum:i5A8FE2A1BEE638DE
GO

Sequence cautioni

The sequence AAH11287 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH69870 differs from that shown. Chimeric cDNA.Curated
The sequence AAN18022 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC38042 differs from that shown. Contaminating sequence. Sequence of unknown origin in the C-terminal part.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti742L → F in BAC38042 (PubMed:16141072).Curated1
Sequence conflicti907R → K in AAN18022 (PubMed:15824097).Curated1
Sequence conflicti907R → K in AAX84653 (PubMed:15824097).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY245539 mRNA. Translation: AAO91933.1.
AY149907 mRNA. Translation: AAN75492.1.
AY147848 mRNA. Translation: AAN18022.1. Different initiation.
AY974091 mRNA. Translation: AAX84653.1.
AK053035 mRNA. Translation: BAC35245.1.
AK080847 mRNA. Translation: BAC38042.1. Sequence problems.
BC011287 mRNA. Translation: AAH11287.1. Different initiation.
BC069870 mRNA. Translation: AAH69870.1. Sequence problems.
BC083072 mRNA. Translation: AAH83072.1.
AK129331 mRNA. Translation: BAC98141.1.
CCDSiCCDS29058.1.
RefSeqiNP_659109.2. NM_144860.2.
XP_006525873.1. XM_006525810.2.
XP_006525874.1. XM_006525811.3.
UniGeneiMm.21500.

Genome annotation databases

EnsembliENSMUST00000052838; ENSMUSP00000054428; ENSMUSG00000024294.
ENSMUST00000165555; ENSMUSP00000131712; ENSMUSG00000024294.
GeneIDi225164.
KEGGimmu:225164.
UCSCiuc008ebc.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiMIB1_MOUSE
AccessioniPrimary (citable) accession number: Q80SY4
Secondary accession number(s): Q5XK51
, Q6IS57, Q6YI52, Q6ZPT8, Q8BNR1, Q8C6W2, Q921Q1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: June 1, 2003
Last modified: November 22, 2017
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways