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Protein

ATP-dependent DNA helicase PIF1

Gene

Pif1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Possesses an intrinsic strand annealing activity.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi237 – 244ATPUniRule annotation8
DNA bindingi586 – 605UniRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase PIF1UniRule annotation (EC:3.6.4.12UniRule annotation)
Alternative name(s):
DNA repair and recombination helicase PIF1UniRule annotation
Pif1/Rrm3 DNA helicase-like protein
Gene namesi
Name:Pif1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:2143057. Pif1.

Subcellular locationi

  • Nucleus UniRule annotation1 Publication
Isoform 3 :

GO - Cellular componenti

  • mitochondrion Source: MGI
  • nuclear chromosome, telomeric region Source: MGI
  • nucleus Source: MGI
  • replication fork Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice do not exhibit any change in telomere length in thymocytes or plenocytes nor significant change in chromosome gain or rearrangements.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002950911 – 650ATP-dependent DNA helicase PIF1Add BLAST650

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei40PhosphoserineBy similarity1
Modified residuei164PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ80SX8.
PRIDEiQ80SX8.

PTM databases

PhosphoSitePlusiQ80SX8.

Expressioni

Gene expression databases

BgeeiENSMUSG00000041064.
ExpressionAtlasiQ80SX8. baseline and differential.
GenevisibleiQ80SX8. MM.

Interactioni

Subunit structurei

Monomer (By similarity). Interacts with telomerase.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000049046.

Structurei

3D structure databases

ProteinModelPortaliQ80SX8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 192PINTBy similarityAdd BLAST179

Domaini

The PIF1 N-terminal (PINT) domain enhances the interaction with ssDNA through intrinsic binding activity, it also harbors DNA strand-annealing activity.By similarity

Sequence similaritiesi

Belongs to the helicase family. PIF1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG0987. Eukaryota.
COG0507. LUCA.
GeneTreeiENSGT00530000063561.
HOGENOMiHOG000132960.
HOVERGENiHBG106706.
InParanoidiQ80SX8.
KOiK15255.
OMAiRACTSGK.
OrthoDBiEOG091G03MY.
PhylomeDBiQ80SX8.
TreeFamiTF319207.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
HAMAPiMF_03176. PIF1. 1 hit.
InterProiIPR003840. DNA_helicase.
IPR010285. DNA_helicase_pif1-like.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23274. PTHR23274. 1 hit.
PfamiPF05970. PIF1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing and alternative initiation. AlignAdd to basket

Isoform 1 (identifier: Q80SX8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRSGLCTPAE ALEMPSSTEA ATDECDDAEL RCRVAVEELS PGGQPRKRQA
60 70 80 90 100
LRAAELSLGR NERRELMLRL QAPGPTGRPR CFPLRAVRLF TRFAATGRST
110 120 130 140 150
LRLPTDGVPG AGSVQLLLSD CPPERLRRFL RTLRLKLAVA PGPGPASARA
160 170 180 190 200
QLLGPRPRDF VTISPVQPEE LQRAAATKAP DSALEKRPME SQTSTEAPRW
210 220 230 240 250
PLPVKKLRMP STKPKLSEEQ AAVLRMVLKG QSIFFTGSAG TGKSYLLKHI
260 270 280 290 300
LGSLPPTGTV ATASTGVAAC HIGGTTLHAF AGIGSGQAPL AQCMALANRP
310 320 330 340 350
GVRQGWLNCQ RLVIDEISMV EADFFDKLEA VARAVRQQKK PFGGIQLIIC
360 370 380 390 400
GDFLQLPPVT KGSQQPQFCF QAKSWRRCVP VILELTEVWR QADQTFISLL
410 420 430 440 450
QAVRLGRCSD EVTRQLRATA AHKVGRDGIV ATRLCTHQDD VALTNEKWLK
460 470 480 490 500
ALPGDVHSFE AIDSDPELSR TLDAQCPVSR VLQLKLGAQV MLVKNLAVSR
510 520 530 540 550
GLVNGARGVV VGFESEGRGL PRVRFLCGIT EVIRTDRWTV QVTGGQYLSR
560 570 580 590 600
QQLPLQLAWA ISIHKSQGMS LDCVEISLGR VFASGQAYVA LSRARSLQGL
610 620 630 640 650
RVLDFDPTVV RCDSRVLHFY ATLRQGRGLS LESQDDEEAN SDLENMDPNL
Length:650
Mass (Da):70,942
Last modified:July 10, 2007 - v2
Checksum:i0330ABA54006DDE4
GO
Isoform 2 (identifier: Q80SX8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     241-272: TGKSYLLKHILGSLPPTGTVATASTGVAACHI → NRAGIRWWAALGGSRQATLTLPTTQGQESPTC
     273-650: Missing.

Show »
Length:272
Mass (Da):29,562
Checksum:i7A1A044ACCEB29C2
GO
Isoform 3 (identifier: Q80SX8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-66: Missing.

Note: Produced by alternative initiation of isoform 1.
Show »
Length:584
Mass (Da):63,731
Checksum:iFE1C6B7C3616AD14
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti79P → L in AAS77397 (PubMed:17130244).Curated1
Sequence conflicti79P → L in AAM50051 (Ref. 2) Curated1
Sequence conflicti79P → L in AAM50052 (Ref. 2) Curated1
Sequence conflicti109P → L in AAS77397 (PubMed:17130244).Curated1
Sequence conflicti109P → L in AAM50051 (Ref. 2) Curated1
Sequence conflicti109P → L in AAM50052 (Ref. 2) Curated1
Sequence conflicti122P → S in AAH46611 (PubMed:15489334).Curated1
Sequence conflicti339K → R in AAH46611 (PubMed:15489334).Curated1
Sequence conflicti367Q → R in AAH46611 (PubMed:15489334).Curated1
Sequence conflicti448W → R in AAH46611 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0474581 – 66Missing in isoform 3. CuratedAdd BLAST66
Alternative sequenceiVSP_026718241 – 272TGKSY…AACHI → NRAGIRWWAALGGSRQATLT LPTTQGQESPTC in isoform 2. 1 PublicationAdd BLAST32
Alternative sequenceiVSP_026719273 – 650Missing in isoform 2. 1 PublicationAdd BLAST378

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY498715 mRNA. Translation: AAS77397.1.
AY100322 mRNA. Translation: AAM50051.1.
AY100323 Genomic DNA. Translation: AAM50052.1.
AK028460 mRNA. Translation: BAC25963.1.
AK049353 mRNA. Translation: BAC33702.1.
BC046611 mRNA. Translation: AAH46611.1.
CCDSiCCDS23295.1. [Q80SX8-1]
RefSeqiNP_766041.1. NM_172453.3. [Q80SX8-1]
XP_006510982.1. XM_006510919.2. [Q80SX8-1]
XP_006510983.1. XM_006510920.2. [Q80SX8-1]
UniGeneiMm.211032.

Genome annotation databases

EnsembliENSMUST00000047099; ENSMUSP00000049046; ENSMUSG00000041064. [Q80SX8-1]
ENSMUST00000131483; ENSMUSP00000117494; ENSMUSG00000041064. [Q80SX8-1]
ENSMUST00000134538; ENSMUSP00000122060; ENSMUSG00000041064. [Q80SX8-1]
GeneIDi208084.
KEGGimmu:208084.
UCSCiuc009qdp.2. mouse. [Q80SX8-1]

Keywords - Coding sequence diversityi

Alternative initiation, Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY498715 mRNA. Translation: AAS77397.1.
AY100322 mRNA. Translation: AAM50051.1.
AY100323 Genomic DNA. Translation: AAM50052.1.
AK028460 mRNA. Translation: BAC25963.1.
AK049353 mRNA. Translation: BAC33702.1.
BC046611 mRNA. Translation: AAH46611.1.
CCDSiCCDS23295.1. [Q80SX8-1]
RefSeqiNP_766041.1. NM_172453.3. [Q80SX8-1]
XP_006510982.1. XM_006510919.2. [Q80SX8-1]
XP_006510983.1. XM_006510920.2. [Q80SX8-1]
UniGeneiMm.211032.

3D structure databases

ProteinModelPortaliQ80SX8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000049046.

PTM databases

PhosphoSitePlusiQ80SX8.

Proteomic databases

PaxDbiQ80SX8.
PRIDEiQ80SX8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047099; ENSMUSP00000049046; ENSMUSG00000041064. [Q80SX8-1]
ENSMUST00000131483; ENSMUSP00000117494; ENSMUSG00000041064. [Q80SX8-1]
ENSMUST00000134538; ENSMUSP00000122060; ENSMUSG00000041064. [Q80SX8-1]
GeneIDi208084.
KEGGimmu:208084.
UCSCiuc009qdp.2. mouse. [Q80SX8-1]

Organism-specific databases

CTDi80119.
MGIiMGI:2143057. Pif1.

Phylogenomic databases

eggNOGiKOG0987. Eukaryota.
COG0507. LUCA.
GeneTreeiENSGT00530000063561.
HOGENOMiHOG000132960.
HOVERGENiHBG106706.
InParanoidiQ80SX8.
KOiK15255.
OMAiRACTSGK.
OrthoDBiEOG091G03MY.
PhylomeDBiQ80SX8.
TreeFamiTF319207.

Miscellaneous databases

PROiQ80SX8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000041064.
ExpressionAtlasiQ80SX8. baseline and differential.
GenevisibleiQ80SX8. MM.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
HAMAPiMF_03176. PIF1. 1 hit.
InterProiIPR003840. DNA_helicase.
IPR010285. DNA_helicase_pif1-like.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23274. PTHR23274. 1 hit.
PfamiPF05970. PIF1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiPIF1_MOUSE
AccessioniPrimary (citable) accession number: Q80SX8
Secondary accession number(s): Q80W44, Q8BIZ3, Q8BJ72
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: November 2, 2016
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.