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Protein

ATP-dependent DNA helicase PIF1

Gene

Pif1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Possesses an intrinsic strand annealing activity.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi237 – 244ATPUniRule annotation8
DNA bindingi586 – 605UniRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA damage, DNA recombination, DNA repair
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase PIF1UniRule annotation (EC:3.6.4.12UniRule annotation)
Alternative name(s):
DNA repair and recombination helicase PIF1UniRule annotation
Pif1/Rrm3 DNA helicase-like protein
Gene namesi
Name:Pif1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:2143057 Pif1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice do not exhibit any change in telomere length in thymocytes or plenocytes nor significant change in chromosome gain or rearrangements.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002950911 – 650ATP-dependent DNA helicase PIF1Add BLAST650

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei40PhosphoserineBy similarity1
Modified residuei164PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ80SX8
PRIDEiQ80SX8

PTM databases

PhosphoSitePlusiQ80SX8

Expressioni

Gene expression databases

BgeeiENSMUSG00000041064
ExpressionAtlasiQ80SX8 baseline and differential
GenevisibleiQ80SX8 MM

Interactioni

Subunit structurei

Monomer (By similarity). Interacts with telomerase.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000049046

Structurei

3D structure databases

ProteinModelPortaliQ80SX8
SMRiQ80SX8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 192PINTBy similarityAdd BLAST179

Domaini

The PIF1 N-terminal (PINT) domain enhances the interaction with ssDNA through intrinsic binding activity, it also harbors DNA strand-annealing activity.By similarity

Sequence similaritiesi

Belongs to the helicase family. PIF1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG0987 Eukaryota
COG0507 LUCA
GeneTreeiENSGT00530000063561
HOGENOMiHOG000132960
HOVERGENiHBG106706
InParanoidiQ80SX8
KOiK15255
OMAiRACTSGK
OrthoDBiEOG091G03MY
PhylomeDBiQ80SX8
TreeFamiTF319207

Family and domain databases

HAMAPiMF_03176 PIF1, 1 hit
InterProiView protein in InterPro
IPR010285 DNA_helicase_pif1-like
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF05970 PIF1, 1 hit
SUPFAMiSSF52540 SSF52540, 3 hits

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing and alternative initiation. AlignAdd to basket

Isoform 1 (identifier: Q80SX8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRSGLCTPAE ALEMPSSTEA ATDECDDAEL RCRVAVEELS PGGQPRKRQA
60 70 80 90 100
LRAAELSLGR NERRELMLRL QAPGPTGRPR CFPLRAVRLF TRFAATGRST
110 120 130 140 150
LRLPTDGVPG AGSVQLLLSD CPPERLRRFL RTLRLKLAVA PGPGPASARA
160 170 180 190 200
QLLGPRPRDF VTISPVQPEE LQRAAATKAP DSALEKRPME SQTSTEAPRW
210 220 230 240 250
PLPVKKLRMP STKPKLSEEQ AAVLRMVLKG QSIFFTGSAG TGKSYLLKHI
260 270 280 290 300
LGSLPPTGTV ATASTGVAAC HIGGTTLHAF AGIGSGQAPL AQCMALANRP
310 320 330 340 350
GVRQGWLNCQ RLVIDEISMV EADFFDKLEA VARAVRQQKK PFGGIQLIIC
360 370 380 390 400
GDFLQLPPVT KGSQQPQFCF QAKSWRRCVP VILELTEVWR QADQTFISLL
410 420 430 440 450
QAVRLGRCSD EVTRQLRATA AHKVGRDGIV ATRLCTHQDD VALTNEKWLK
460 470 480 490 500
ALPGDVHSFE AIDSDPELSR TLDAQCPVSR VLQLKLGAQV MLVKNLAVSR
510 520 530 540 550
GLVNGARGVV VGFESEGRGL PRVRFLCGIT EVIRTDRWTV QVTGGQYLSR
560 570 580 590 600
QQLPLQLAWA ISIHKSQGMS LDCVEISLGR VFASGQAYVA LSRARSLQGL
610 620 630 640 650
RVLDFDPTVV RCDSRVLHFY ATLRQGRGLS LESQDDEEAN SDLENMDPNL
Length:650
Mass (Da):70,942
Last modified:July 10, 2007 - v2
Checksum:i0330ABA54006DDE4
GO
Isoform 2 (identifier: Q80SX8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     241-272: TGKSYLLKHILGSLPPTGTVATASTGVAACHI → NRAGIRWWAALGGSRQATLTLPTTQGQESPTC
     273-650: Missing.

Show »
Length:272
Mass (Da):29,562
Checksum:i7A1A044ACCEB29C2
GO
Isoform 3 (identifier: Q80SX8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-66: Missing.

Note: Produced by alternative initiation of isoform 1.
Show »
Length:584
Mass (Da):63,731
Checksum:iFE1C6B7C3616AD14
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti79P → L in AAS77397 (PubMed:17130244).Curated1
Sequence conflicti79P → L in AAM50051 (Ref. 2) Curated1
Sequence conflicti79P → L in AAM50052 (Ref. 2) Curated1
Sequence conflicti109P → L in AAS77397 (PubMed:17130244).Curated1
Sequence conflicti109P → L in AAM50051 (Ref. 2) Curated1
Sequence conflicti109P → L in AAM50052 (Ref. 2) Curated1
Sequence conflicti122P → S in AAH46611 (PubMed:15489334).Curated1
Sequence conflicti339K → R in AAH46611 (PubMed:15489334).Curated1
Sequence conflicti367Q → R in AAH46611 (PubMed:15489334).Curated1
Sequence conflicti448W → R in AAH46611 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0474581 – 66Missing in isoform 3. CuratedAdd BLAST66
Alternative sequenceiVSP_026718241 – 272TGKSY…AACHI → NRAGIRWWAALGGSRQATLT LPTTQGQESPTC in isoform 2. 1 PublicationAdd BLAST32
Alternative sequenceiVSP_026719273 – 650Missing in isoform 2. 1 PublicationAdd BLAST378

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY498715 mRNA Translation: AAS77397.1
AY100322 mRNA Translation: AAM50051.1
AY100323 Genomic DNA Translation: AAM50052.1
AK028460 mRNA Translation: BAC25963.1
AK049353 mRNA Translation: BAC33702.1
BC046611 mRNA Translation: AAH46611.1
CCDSiCCDS23295.1 [Q80SX8-1]
RefSeqiNP_766041.1, NM_172453.3 [Q80SX8-1]
XP_006510982.1, XM_006510919.2
XP_006510983.1, XM_006510920.2
UniGeneiMm.211032

Genome annotation databases

EnsembliENSMUST00000047099; ENSMUSP00000049046; ENSMUSG00000041064 [Q80SX8-1]
ENSMUST00000131483; ENSMUSP00000117494; ENSMUSG00000041064 [Q80SX8-1]
ENSMUST00000134538; ENSMUSP00000122060; ENSMUSG00000041064 [Q80SX8-1]
GeneIDi208084
KEGGimmu:208084
UCSCiuc009qdp.2 mouse [Q80SX8-1]

Keywords - Coding sequence diversityi

Alternative initiation, Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPIF1_MOUSE
AccessioniPrimary (citable) accession number: Q80SX8
Secondary accession number(s): Q80W44, Q8BIZ3, Q8BJ72
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: April 25, 2018
This is version 102 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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