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Protein

ATP-dependent DNA helicase PIF1

Gene

Pif1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Possesses an intrinsic strand annealing activity.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi237 – 2448ATPUniRule annotation
DNA bindingi586 – 60520UniRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase PIF1UniRule annotation (EC:3.6.4.12UniRule annotation)
Alternative name(s):
DNA repair and recombination helicase PIF1UniRule annotation
Pif1/Rrm3 DNA helicase-like protein
Gene namesi
Name:Pif1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:2143057. Pif1.

Subcellular locationi

  • Nucleus UniRule annotation1 Publication
Isoform 3 :

GO - Cellular componenti

  • mitochondrion Source: MGI
  • nuclear chromosome, telomeric region Source: MGI
  • nucleus Source: MGI
  • replication fork Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice do not exhibit any change in telomere length in thymocytes or plenocytes nor significant change in chromosome gain or rearrangements.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 650650ATP-dependent DNA helicase PIF1PRO_0000295091Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei40 – 401PhosphoserineBy similarity
Modified residuei164 – 1641PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ80SX8.
PRIDEiQ80SX8.

PTM databases

PhosphoSiteiQ80SX8.

Expressioni

Gene expression databases

BgeeiQ80SX8.
ExpressionAtlasiQ80SX8. baseline and differential.
GenevisibleiQ80SX8. MM.

Interactioni

Subunit structurei

Monomer (By similarity). Interacts with telomerase.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000049046.

Structurei

3D structure databases

ProteinModelPortaliQ80SX8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni14 – 192179PINTBy similarityAdd
BLAST

Domaini

The PIF1 N-terminal (PINT) domain enhances the interaction with ssDNA through intrinsic binding activity, it also harbors DNA strand-annealing activity.By similarity

Sequence similaritiesi

Belongs to the helicase family. PIF1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG0987. Eukaryota.
COG0507. LUCA.
GeneTreeiENSGT00530000063561.
HOGENOMiHOG000132960.
HOVERGENiHBG106706.
InParanoidiQ80SX8.
KOiK15255.
OMAiRACTSGK.
OrthoDBiEOG7RZ5PJ.
PhylomeDBiQ80SX8.
TreeFamiTF319207.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
HAMAPiMF_03176. PIF1.
InterProiIPR003840. DNA_helicase.
IPR010285. DNA_helicase_pif1-like.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23274. PTHR23274. 1 hit.
PfamiPF05970. PIF1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing and alternative initiation. AlignAdd to basket

Isoform 1 (identifier: Q80SX8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRSGLCTPAE ALEMPSSTEA ATDECDDAEL RCRVAVEELS PGGQPRKRQA
60 70 80 90 100
LRAAELSLGR NERRELMLRL QAPGPTGRPR CFPLRAVRLF TRFAATGRST
110 120 130 140 150
LRLPTDGVPG AGSVQLLLSD CPPERLRRFL RTLRLKLAVA PGPGPASARA
160 170 180 190 200
QLLGPRPRDF VTISPVQPEE LQRAAATKAP DSALEKRPME SQTSTEAPRW
210 220 230 240 250
PLPVKKLRMP STKPKLSEEQ AAVLRMVLKG QSIFFTGSAG TGKSYLLKHI
260 270 280 290 300
LGSLPPTGTV ATASTGVAAC HIGGTTLHAF AGIGSGQAPL AQCMALANRP
310 320 330 340 350
GVRQGWLNCQ RLVIDEISMV EADFFDKLEA VARAVRQQKK PFGGIQLIIC
360 370 380 390 400
GDFLQLPPVT KGSQQPQFCF QAKSWRRCVP VILELTEVWR QADQTFISLL
410 420 430 440 450
QAVRLGRCSD EVTRQLRATA AHKVGRDGIV ATRLCTHQDD VALTNEKWLK
460 470 480 490 500
ALPGDVHSFE AIDSDPELSR TLDAQCPVSR VLQLKLGAQV MLVKNLAVSR
510 520 530 540 550
GLVNGARGVV VGFESEGRGL PRVRFLCGIT EVIRTDRWTV QVTGGQYLSR
560 570 580 590 600
QQLPLQLAWA ISIHKSQGMS LDCVEISLGR VFASGQAYVA LSRARSLQGL
610 620 630 640 650
RVLDFDPTVV RCDSRVLHFY ATLRQGRGLS LESQDDEEAN SDLENMDPNL
Length:650
Mass (Da):70,942
Last modified:July 10, 2007 - v2
Checksum:i0330ABA54006DDE4
GO
Isoform 2 (identifier: Q80SX8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     241-272: TGKSYLLKHILGSLPPTGTVATASTGVAACHI → NRAGIRWWAALGGSRQATLTLPTTQGQESPTC
     273-650: Missing.

Show »
Length:272
Mass (Da):29,562
Checksum:i7A1A044ACCEB29C2
GO
Isoform 3 (identifier: Q80SX8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-66: Missing.

Note: Produced by alternative initiation of isoform 1.
Show »
Length:584
Mass (Da):63,731
Checksum:iFE1C6B7C3616AD14
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti79 – 791P → L in AAS77397 (PubMed:17130244).Curated
Sequence conflicti79 – 791P → L in AAM50051 (Ref. 2) Curated
Sequence conflicti79 – 791P → L in AAM50052 (Ref. 2) Curated
Sequence conflicti109 – 1091P → L in AAS77397 (PubMed:17130244).Curated
Sequence conflicti109 – 1091P → L in AAM50051 (Ref. 2) Curated
Sequence conflicti109 – 1091P → L in AAM50052 (Ref. 2) Curated
Sequence conflicti122 – 1221P → S in AAH46611 (PubMed:15489334).Curated
Sequence conflicti339 – 3391K → R in AAH46611 (PubMed:15489334).Curated
Sequence conflicti367 – 3671Q → R in AAH46611 (PubMed:15489334).Curated
Sequence conflicti448 – 4481W → R in AAH46611 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6666Missing in isoform 3. CuratedVSP_047458Add
BLAST
Alternative sequencei241 – 27232TGKSY…AACHI → NRAGIRWWAALGGSRQATLT LPTTQGQESPTC in isoform 2. 1 PublicationVSP_026718Add
BLAST
Alternative sequencei273 – 650378Missing in isoform 2. 1 PublicationVSP_026719Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY498715 mRNA. Translation: AAS77397.1.
AY100322 mRNA. Translation: AAM50051.1.
AY100323 Genomic DNA. Translation: AAM50052.1.
AK028460 mRNA. Translation: BAC25963.1.
AK049353 mRNA. Translation: BAC33702.1.
BC046611 mRNA. Translation: AAH46611.1.
CCDSiCCDS23295.1. [Q80SX8-1]
RefSeqiNP_766041.1. NM_172453.3. [Q80SX8-1]
XP_006510982.1. XM_006510919.2. [Q80SX8-1]
XP_006510983.1. XM_006510920.2. [Q80SX8-1]
UniGeneiMm.211032.

Genome annotation databases

EnsembliENSMUST00000047099; ENSMUSP00000049046; ENSMUSG00000041064. [Q80SX8-1]
ENSMUST00000131483; ENSMUSP00000117494; ENSMUSG00000041064. [Q80SX8-1]
ENSMUST00000134538; ENSMUSP00000122060; ENSMUSG00000041064. [Q80SX8-1]
GeneIDi208084.
KEGGimmu:208084.
UCSCiuc009qdp.2. mouse. [Q80SX8-1]

Keywords - Coding sequence diversityi

Alternative initiation, Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY498715 mRNA. Translation: AAS77397.1.
AY100322 mRNA. Translation: AAM50051.1.
AY100323 Genomic DNA. Translation: AAM50052.1.
AK028460 mRNA. Translation: BAC25963.1.
AK049353 mRNA. Translation: BAC33702.1.
BC046611 mRNA. Translation: AAH46611.1.
CCDSiCCDS23295.1. [Q80SX8-1]
RefSeqiNP_766041.1. NM_172453.3. [Q80SX8-1]
XP_006510982.1. XM_006510919.2. [Q80SX8-1]
XP_006510983.1. XM_006510920.2. [Q80SX8-1]
UniGeneiMm.211032.

3D structure databases

ProteinModelPortaliQ80SX8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000049046.

PTM databases

PhosphoSiteiQ80SX8.

Proteomic databases

PaxDbiQ80SX8.
PRIDEiQ80SX8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047099; ENSMUSP00000049046; ENSMUSG00000041064. [Q80SX8-1]
ENSMUST00000131483; ENSMUSP00000117494; ENSMUSG00000041064. [Q80SX8-1]
ENSMUST00000134538; ENSMUSP00000122060; ENSMUSG00000041064. [Q80SX8-1]
GeneIDi208084.
KEGGimmu:208084.
UCSCiuc009qdp.2. mouse. [Q80SX8-1]

Organism-specific databases

CTDi80119.
MGIiMGI:2143057. Pif1.

Phylogenomic databases

eggNOGiKOG0987. Eukaryota.
COG0507. LUCA.
GeneTreeiENSGT00530000063561.
HOGENOMiHOG000132960.
HOVERGENiHBG106706.
InParanoidiQ80SX8.
KOiK15255.
OMAiRACTSGK.
OrthoDBiEOG7RZ5PJ.
PhylomeDBiQ80SX8.
TreeFamiTF319207.

Miscellaneous databases

PROiQ80SX8.
SOURCEiSearch...

Gene expression databases

BgeeiQ80SX8.
ExpressionAtlasiQ80SX8. baseline and differential.
GenevisibleiQ80SX8. MM.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
HAMAPiMF_03176. PIF1.
InterProiIPR003840. DNA_helicase.
IPR010285. DNA_helicase_pif1-like.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23274. PTHR23274. 1 hit.
PfamiPF05970. PIF1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Murine Pif1 interacts with telomerase and is dispensable for telomere function in vivo."
    Snow B.E., Mateyak M., Paderova J., Wakeham A., Iorio C., Zakian V., Squire J., Harrington L.
    Mol. Cell. Biol. 27:1017-1026(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, INTERACTION WITH TELOMERASE.
  2. "Assignment of the mouse Pif1 gene to mouse chromosome 9D by FISH and interspecific backcross analyses."
    Lo A.W.I., Murnane J.P.
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: 129/SvJ.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Skin.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Colon.
  5. Cited for: ALTERNATIVE INITIATION (ISOFORM 3), SUBCELLULAR LOCATION (ISOFORM 3).

Entry informationi

Entry nameiPIF1_MOUSE
AccessioniPrimary (citable) accession number: Q80SX8
Secondary accession number(s): Q80W44, Q8BIZ3, Q8BJ72
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: July 6, 2016
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.