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Protein

Putative adenosylhomocysteinase 2

Gene

Ahcyl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a (dATP)-dependent inhibitor of ribonucleotide reductase large subunit RRM1, controlling the endogenous dNTP pool and ensuring normal cell cycle progression.By similarity

Catalytic activityi

S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine.

Cofactori

NAD(+)By similarityNote: Binds 1 NAD(+) per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei155 – 1551SubstrateBy similarity
Binding sitei229 – 2291SubstrateBy similarity
Binding sitei254 – 2541SubstrateBy similarity
Binding sitei284 – 2841SubstrateBy similarity
Binding sitei288 – 2881SubstrateBy similarity
Binding sitei341 – 3411NADBy similarity
Binding sitei376 – 3761NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi318 – 3225NADBy similarity
Nucleotide bindingi397 – 3993NADBy similarity

GO - Molecular functioni

  1. hydrolase activity Source: UniProtKB-KW

GO - Biological processi

  1. mRNA polyadenylation Source: MGI
  2. one-carbon metabolic process Source: UniProtKB-KW
  3. positive regulation of sodium ion transport Source: MGI
  4. protein export from nucleus Source: MGI
  5. regulation of anion transport Source: MGI
  6. regulation of ion transmembrane transporter activity Source: MGI
  7. regulation of mRNA 3'-end processing Source: MGI
  8. S-adenosylmethionine cycle Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00314; UER00076.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative adenosylhomocysteinase 2 (EC:3.3.1.1)
Short name:
AdoHcyase 2
Alternative name(s):
IP3R-binding protein released with inositol 1,4,5-trisphosphate
S-adenosyl-L-homocysteine hydrolase 2
S-adenosylhomocysteine hydrolase-like protein 1
Gene namesi
Name:Ahcyl1
Synonyms:Irbit
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:2385184. Ahcyl1.

Subcellular locationi

Endoplasmic reticulum 1 Publication

GO - Cellular componenti

  1. cytosol Source: GO_Central
  2. endoplasmic reticulum Source: UniProtKB-SubCell
  3. extracellular vesicular exosome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi70 – 734Missing : Inhibits interaction with ITPR1. 1 Publication
Mutagenesisi73 – 731D → R: Inhibits interaction with ITPR1. 1 Publication
Mutagenesisi82 – 887Missing : Inhibits interaction with ITPR1. 1 Publication
Mutagenesisi86 – 883DDE → KKK: Does not inhibits interaction with ITPR1. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 530529Putative adenosylhomocysteinase 2PRO_0000230300Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei2 – 21PhosphoserineBy similarity
Modified residuei40 – 401N6-acetyllysine1 Publication
Modified residuei391 – 3911PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated at Ser/Thr residues between Ser-68 and Thr-72 in the PEST region: required for interaction with dATP-bound RRM1.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ80SW1.
PaxDbiQ80SW1.
PRIDEiQ80SW1.

PTM databases

PhosphoSiteiQ80SW1.

Expressioni

Gene expression databases

BgeeiQ80SW1.
CleanExiMM_AHCYL1.
ExpressionAtlasiQ80SW1. baseline and differential.
GenevestigatoriQ80SW1.

Interactioni

Subunit structurei

Interacts with RRM1, in a phosphorylation- and (dATP)-dependent manner (By similarity). Interacts with ITPR1. Competes with IP3 for interaction with ITPR1 and attenuates IP3-induced Ca2+ release through the ITPR1 from the non-mitochondrial intracellular stores.By similarity2 Publications

Protein-protein interaction databases

IntActiQ80SW1. 1 interaction.
MINTiMINT-4437427.
STRINGi10090.ENSMUSP00000029490.

Structurei

3D structure databases

ProteinModelPortaliQ80SW1.
SMRiQ80SW1. Positions 101-530.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni65 – 9228PESTAdd
BLAST
Regioni281 – 448168NAD bindingBy similarityAdd
BLAST
Regioni520 – 53011PDZ-bindingAdd
BLAST

Domaini

The PEST region is essential for the interaction with ITPR1, and, when phosphorylated, is also the RRM1-binding region. The PDZ-binding region is required for maximal interaction with ITPR1 and is also responsible for the IP3-insensitive interaction with ITPR1.

Sequence similaritiesi

Belongs to the adenosylhomocysteinase family.Curated

Phylogenomic databases

eggNOGiCOG0499.
GeneTreeiENSGT00390000003626.
HOGENOMiHOG000227986.
HOVERGENiHBG005041.
InParanoidiQ80SW1.
KOiK01251.
OMAiPVKKQIQ.
PhylomeDBiQ80SW1.
TreeFamiTF300415.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR000043. Adenosylhomocysteinase.
IPR015878. Ado_hCys_hydrolase_NAD-bd.
IPR016040. NAD(P)-bd_dom.
IPR020082. S-Ado-L-homoCys_hydrolase_CS.
[Graphical view]
PANTHERiPTHR23420. PTHR23420. 1 hit.
PfamiPF05221. AdoHcyase. 1 hit.
PF00670. AdoHcyase_NAD. 1 hit.
[Graphical view]
PIRSFiPIRSF001109. Ad_hcy_hydrolase. 1 hit.
SMARTiSM00996. AdoHcyase. 1 hit.
SM00997. AdoHcyase_NAD. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00936. ahcY. 1 hit.
PROSITEiPS00738. ADOHCYASE_1. 1 hit.
PS00739. ADOHCYASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q80SW1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMPDAMPLP GVGEELKQAK EIEDAEKYSF MATVTKAPKK QIQFADDMQE
60 70 80 90 100
FTKFPTKTGR RSLSRSISQS STDSYSSAAS YTDSSDDEVS PREKQQTNSK
110 120 130 140 150
GSSNFCVKNI KQAEFGRREI EIAEQDMSAL ISLRKRAQGE KPLAGAKIVG
160 170 180 190 200
CTHITAQTAV LIETLCALGA QCRWSACNIY STQNEVAAAL AEAGVAVFAW
210 220 230 240 250
KGESEDDFWW CIDRCVNMDG WQANMILDDG GDLTHWVYKK YPNVFKKIRG
260 270 280 290 300
IVEESVTGVH RLYQLSKAGK LCVPAMNVND SVTKQKFDNL YCCRESILDG
310 320 330 340 350
LKRTTDVMFG GKQVVVCGYG EVGKGCCAAL KALGAIVYIT EIDPICALQA
360 370 380 390 400
CMDGFRVVKL NEVIRQVDVV ITCTGNKNVV TREHLDRMKN SCIVCNMGHS
410 420 430 440 450
NTEIDVTSLR TPELTWERVR SQVDHVIWPD GKRVVLLAEG RLLNLSCSTV
460 470 480 490 500
PTFVLSITAT TQALALIELY NAPEGRYKQD VYLLPKKMDE YVASLHLPSF
510 520 530
DAHLTELTDD QAKYLGLNKN GPFKPNYYRY
Length:530
Mass (Da):58,951
Last modified:June 1, 2003 - v1
Checksum:i974D23361A245D04
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB092504 mRNA. Translation: BAC65166.1.
BC018218 mRNA. Translation: AAH18218.2.
BK000547 mRNA. Translation: DAA00059.1.
CCDSiCCDS38593.1.
RefSeqiNP_663517.2. NM_145542.3.
UniGeneiMm.220328.

Genome annotation databases

EnsembliENSMUST00000029490; ENSMUSP00000029490; ENSMUSG00000027893.
GeneIDi229709.
KEGGimmu:229709.
UCSCiuc008qxi.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB092504 mRNA. Translation: BAC65166.1.
BC018218 mRNA. Translation: AAH18218.2.
BK000547 mRNA. Translation: DAA00059.1.
CCDSiCCDS38593.1.
RefSeqiNP_663517.2. NM_145542.3.
UniGeneiMm.220328.

3D structure databases

ProteinModelPortaliQ80SW1.
SMRiQ80SW1. Positions 101-530.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ80SW1. 1 interaction.
MINTiMINT-4437427.
STRINGi10090.ENSMUSP00000029490.

PTM databases

PhosphoSiteiQ80SW1.

Proteomic databases

MaxQBiQ80SW1.
PaxDbiQ80SW1.
PRIDEiQ80SW1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029490; ENSMUSP00000029490; ENSMUSG00000027893.
GeneIDi229709.
KEGGimmu:229709.
UCSCiuc008qxi.1. mouse.

Organism-specific databases

CTDi10768.
MGIiMGI:2385184. Ahcyl1.

Phylogenomic databases

eggNOGiCOG0499.
GeneTreeiENSGT00390000003626.
HOGENOMiHOG000227986.
HOVERGENiHBG005041.
InParanoidiQ80SW1.
KOiK01251.
OMAiPVKKQIQ.
PhylomeDBiQ80SW1.
TreeFamiTF300415.

Enzyme and pathway databases

UniPathwayiUPA00314; UER00076.

Miscellaneous databases

NextBioi379631.
PROiQ80SW1.
SOURCEiSearch...

Gene expression databases

BgeeiQ80SW1.
CleanExiMM_AHCYL1.
ExpressionAtlasiQ80SW1. baseline and differential.
GenevestigatoriQ80SW1.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR000043. Adenosylhomocysteinase.
IPR015878. Ado_hCys_hydrolase_NAD-bd.
IPR016040. NAD(P)-bd_dom.
IPR020082. S-Ado-L-homoCys_hydrolase_CS.
[Graphical view]
PANTHERiPTHR23420. PTHR23420. 1 hit.
PfamiPF05221. AdoHcyase. 1 hit.
PF00670. AdoHcyase_NAD. 1 hit.
[Graphical view]
PIRSFiPIRSF001109. Ad_hcy_hydrolase. 1 hit.
SMARTiSM00996. AdoHcyase. 1 hit.
SM00997. AdoHcyase_NAD. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00936. ahcY. 1 hit.
PROSITEiPS00738. ADOHCYASE_1. 1 hit.
PS00739. ADOHCYASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "IRBIT, a novel inositol 1,4,5-trisphosphate (IP3) receptor-binding protein, is released from the IP3 receptor upon IP3 binding to the receptor."
    Ando H., Mizutani A., Matsu-ura T., Mikoshiba K.
    J. Biol. Chem. 278:10602-10612(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH ITPR1.
    Tissue: Cerebellum.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary tumor.
  3. Cited for: PROTEIN SEQUENCE OF 74-80, INTERACTION WITH ITPR1, MUTAGENESIS OF 70-SER--ASP-73; ASP-73; 82-THR--ASP-88 AND 86-ASP--GLU-88, SUBCELLULAR LOCATION.
  4. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (DEC-2008) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 479-486, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  5. "Identification of an S-adenosylhomocysteine hydrolase-like transcript induced during dendritic cell differentiation."
    Dekker J.W., Budhia S., Angel N.Z., Cooper B.J., Clark G.J., Hart D.N., Kato M.
    Immunogenetics 53:993-1001(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  7. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-40, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiSAHH2_MOUSE
AccessioniPrimary (citable) accession number: Q80SW1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: June 1, 2003
Last modified: March 4, 2015
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.