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Q80SW1 (SAHH2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative adenosylhomocysteinase 2

Short name=AdoHcyase 2
EC=3.3.1.1
Alternative name(s):
IP3R-binding protein released with inositol 1,4,5-trisphosphate
S-adenosyl-L-homocysteine hydrolase 2
S-adenosylhomocysteine hydrolase-like protein 1
Gene names
Name:Ahcyl1
Synonyms:Irbit
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length530 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine.

Cofactor

Binds 1 NAD per subunit By similarity.

Pathway

Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.

Subunit structure

Interacts with ITPR1. Competes with IP3 for interaction with ITPR1 and attenuates IP3-induced Ca2+ release through the ITPR1 from the non-mitochondrial intracellular stores. Ref.1 Ref.3

Subcellular location

Endoplasmic reticulum Ref.3.

Domain

The PEST region is essential for the interaction with ITPR1. The PDZ-binding region is required for maximal interaction with ITPR1 and is also responsible for the IP3-insensitive interaction with ITPR1.

Sequence similarities

Belongs to the adenosylhomocysteinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 530529Putative adenosylhomocysteinase 2
PRO_0000230300

Regions

Nucleotide binding318 – 3225NAD By similarity
Nucleotide binding397 – 3993NAD By similarity
Region65 – 9228PEST
Region281 – 448168NAD binding By similarity
Region520 – 53011PDZ-binding

Sites

Binding site1551Substrate By similarity
Binding site2291Substrate By similarity
Binding site2541Substrate By similarity
Binding site2841Substrate By similarity
Binding site2881Substrate By similarity
Binding site3411NAD By similarity
Binding site3761NAD By similarity

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue21Phosphoserine By similarity
Modified residue401N6-acetyllysine Ref.7
Modified residue3911Phosphoserine By similarity

Experimental info

Mutagenesis70 – 734Missing: Inhibits interaction with ITPR1. Ref.3
Mutagenesis731D → R: Inhibits interaction with ITPR1. Ref.3
Mutagenesis82 – 887Missing: Inhibits interaction with ITPR1. Ref.3
Mutagenesis86 – 883DDE → KKK: Does not inhibits interaction with ITPR1. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q80SW1 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 974D23361A245D04

FASTA53058,951
        10         20         30         40         50         60 
MSMPDAMPLP GVGEELKQAK EIEDAEKYSF MATVTKAPKK QIQFADDMQE FTKFPTKTGR 

        70         80         90        100        110        120 
RSLSRSISQS STDSYSSAAS YTDSSDDEVS PREKQQTNSK GSSNFCVKNI KQAEFGRREI 

       130        140        150        160        170        180 
EIAEQDMSAL ISLRKRAQGE KPLAGAKIVG CTHITAQTAV LIETLCALGA QCRWSACNIY 

       190        200        210        220        230        240 
STQNEVAAAL AEAGVAVFAW KGESEDDFWW CIDRCVNMDG WQANMILDDG GDLTHWVYKK 

       250        260        270        280        290        300 
YPNVFKKIRG IVEESVTGVH RLYQLSKAGK LCVPAMNVND SVTKQKFDNL YCCRESILDG 

       310        320        330        340        350        360 
LKRTTDVMFG GKQVVVCGYG EVGKGCCAAL KALGAIVYIT EIDPICALQA CMDGFRVVKL 

       370        380        390        400        410        420 
NEVIRQVDVV ITCTGNKNVV TREHLDRMKN SCIVCNMGHS NTEIDVTSLR TPELTWERVR 

       430        440        450        460        470        480 
SQVDHVIWPD GKRVVLLAEG RLLNLSCSTV PTFVLSITAT TQALALIELY NAPEGRYKQD 

       490        500        510        520        530 
VYLLPKKMDE YVASLHLPSF DAHLTELTDD QAKYLGLNKN GPFKPNYYRY 

« Hide

References

« Hide 'large scale' references
[1]"IRBIT, a novel inositol 1,4,5-trisphosphate (IP3) receptor-binding protein, is released from the IP3 receptor upon IP3 binding to the receptor."
Ando H., Mizutani A., Matsu-ura T., Mikoshiba K.
J. Biol. Chem. 278:10602-10612(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH ITPR1.
Tissue: Cerebellum.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Czech II.
Tissue: Mammary tumor.
[3]"Binding of IRBIT to the IP3 receptor: determinants and functional effects."
Devogelaere B., Nadif Kasri N., Derua R., Waelkens E., Callewaert G., Missiaen L., Parys J.B., De Smedt H.
Biochem. Biophys. Res. Commun. 343:49-56(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 74-80, INTERACTION WITH ITPR1, MUTAGENESIS OF 70-SER--ASP-73; ASP-73; 82-THR--ASP-88 AND 86-ASP--GLU-88, SUBCELLULAR LOCATION.
[4]Lubec G., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 479-486, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: OF1.
Tissue: Hippocampus.
[5]"Identification of an S-adenosylhomocysteine hydrolase-like transcript induced during dendritic cell differentiation."
Dekker J.W., Budhia S., Angel N.Z., Cooper B.J., Clark G.J., Hart D.N., Kato M.
Immunogenetics 53:993-1001(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
[6]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[7]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-40, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB092504 mRNA. Translation: BAC65166.1.
BC018218 mRNA. Translation: AAH18218.2.
BK000547 mRNA. Translation: DAA00059.1.
CCDSCCDS38593.1.
RefSeqNP_663517.2. NM_145542.3.
UniGeneMm.220328.

3D structure databases

ProteinModelPortalQ80SW1.
SMRQ80SW1. Positions 101-530.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ80SW1. 1 interaction.
MINTMINT-4437427.
STRING10090.ENSMUSP00000029490.

PTM databases

PhosphoSiteQ80SW1.

Proteomic databases

MaxQBQ80SW1.
PaxDbQ80SW1.
PRIDEQ80SW1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000029490; ENSMUSP00000029490; ENSMUSG00000027893.
GeneID229709.
KEGGmmu:229709.
UCSCuc008qxi.1. mouse.

Organism-specific databases

CTD10768.
MGIMGI:2385184. Ahcyl1.

Phylogenomic databases

eggNOGCOG0499.
GeneTreeENSGT00390000003626.
HOGENOMHOG000227986.
HOVERGENHBG005041.
InParanoidQ80SW1.
KOK01251.
OMAKQIQFVE.
PhylomeDBQ80SW1.
TreeFamTF300415.

Enzyme and pathway databases

UniPathwayUPA00314; UER00076.

Gene expression databases

ArrayExpressQ80SW1.
BgeeQ80SW1.
CleanExMM_AHCYL1.
GenevestigatorQ80SW1.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR000043. Adenosylhomocysteinase.
IPR015878. Ado_hCys_hydrolase_NAD-bd.
IPR016040. NAD(P)-bd_dom.
IPR020082. S-Ado-L-homoCys_hydrolase_CS.
[Graphical view]
PANTHERPTHR23420. PTHR23420. 1 hit.
PfamPF05221. AdoHcyase. 1 hit.
PF00670. AdoHcyase_NAD. 1 hit.
[Graphical view]
PIRSFPIRSF001109. Ad_hcy_hydrolase. 1 hit.
SMARTSM00996. AdoHcyase. 1 hit.
SM00997. AdoHcyase_NAD. 1 hit.
[Graphical view]
TIGRFAMsTIGR00936. ahcY. 1 hit.
PROSITEPS00738. ADOHCYASE_1. 1 hit.
PS00739. ADOHCYASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio379631.
PROQ80SW1.
SOURCESearch...

Entry information

Entry nameSAHH2_MOUSE
AccessionPrimary (citable) accession number: Q80SW1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot