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Q80SW1

- SAHH2_MOUSE

UniProt

Q80SW1 - SAHH2_MOUSE

Protein

Putative adenosylhomocysteinase 2

Gene

Ahcyl1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine.

    Cofactori

    Binds 1 NAD per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei155 – 1551SubstrateBy similarity
    Binding sitei229 – 2291SubstrateBy similarity
    Binding sitei254 – 2541SubstrateBy similarity
    Binding sitei284 – 2841SubstrateBy similarity
    Binding sitei288 – 2881SubstrateBy similarity
    Binding sitei341 – 3411NADBy similarity
    Binding sitei376 – 3761NADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi318 – 3225NADBy similarity
    Nucleotide bindingi397 – 3993NADBy similarity

    GO - Molecular functioni

    1. adenosylhomocysteinase activity Source: UniProtKB-EC
    2. protein binding Source: MGI

    GO - Biological processi

    1. mRNA polyadenylation Source: MGI
    2. one-carbon metabolic process Source: UniProtKB-KW
    3. positive regulation of sodium ion transport Source: MGI
    4. protein export from nucleus Source: MGI
    5. regulation of anion transport Source: MGI
    6. regulation of ion transmembrane transporter activity Source: MGI
    7. regulation of mRNA 3'-end processing Source: MGI

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    One-carbon metabolism

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    UniPathwayiUPA00314; UER00076.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative adenosylhomocysteinase 2 (EC:3.3.1.1)
    Short name:
    AdoHcyase 2
    Alternative name(s):
    IP3R-binding protein released with inositol 1,4,5-trisphosphate
    S-adenosyl-L-homocysteine hydrolase 2
    S-adenosylhomocysteine hydrolase-like protein 1
    Gene namesi
    Name:Ahcyl1
    Synonyms:Irbit
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:2385184. Ahcyl1.

    Subcellular locationi

    Endoplasmic reticulum 1 Publication

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi70 – 734Missing: Inhibits interaction with ITPR1.
    Mutagenesisi73 – 731D → R: Inhibits interaction with ITPR1. 1 Publication
    Mutagenesisi82 – 887Missing: Inhibits interaction with ITPR1.
    Mutagenesisi86 – 883DDE → KKK: Does not inhibits interaction with ITPR1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 530529Putative adenosylhomocysteinase 2PRO_0000230300Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei2 – 21PhosphoserineBy similarity
    Modified residuei40 – 401N6-acetyllysine1 Publication
    Modified residuei391 – 3911PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ80SW1.
    PaxDbiQ80SW1.
    PRIDEiQ80SW1.

    PTM databases

    PhosphoSiteiQ80SW1.

    Expressioni

    Gene expression databases

    ArrayExpressiQ80SW1.
    BgeeiQ80SW1.
    CleanExiMM_AHCYL1.
    GenevestigatoriQ80SW1.

    Interactioni

    Subunit structurei

    Interacts with ITPR1. Competes with IP3 for interaction with ITPR1 and attenuates IP3-induced Ca2+ release through the ITPR1 from the non-mitochondrial intracellular stores.2 Publications

    Protein-protein interaction databases

    IntActiQ80SW1. 1 interaction.
    MINTiMINT-4437427.
    STRINGi10090.ENSMUSP00000029490.

    Structurei

    3D structure databases

    ProteinModelPortaliQ80SW1.
    SMRiQ80SW1. Positions 101-530.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni65 – 9228PESTAdd
    BLAST
    Regioni281 – 448168NAD bindingBy similarityAdd
    BLAST
    Regioni520 – 53011PDZ-bindingAdd
    BLAST

    Domaini

    The PEST region is essential for the interaction with ITPR1. The PDZ-binding region is required for maximal interaction with ITPR1 and is also responsible for the IP3-insensitive interaction with ITPR1.

    Sequence similaritiesi

    Belongs to the adenosylhomocysteinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0499.
    GeneTreeiENSGT00390000003626.
    HOGENOMiHOG000227986.
    HOVERGENiHBG005041.
    InParanoidiQ80SW1.
    KOiK01251.
    OMAiKQIQFVE.
    PhylomeDBiQ80SW1.
    TreeFamiTF300415.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR000043. Adenosylhomocysteinase.
    IPR015878. Ado_hCys_hydrolase_NAD-bd.
    IPR016040. NAD(P)-bd_dom.
    IPR020082. S-Ado-L-homoCys_hydrolase_CS.
    [Graphical view]
    PANTHERiPTHR23420. PTHR23420. 1 hit.
    PfamiPF05221. AdoHcyase. 1 hit.
    PF00670. AdoHcyase_NAD. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001109. Ad_hcy_hydrolase. 1 hit.
    SMARTiSM00996. AdoHcyase. 1 hit.
    SM00997. AdoHcyase_NAD. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00936. ahcY. 1 hit.
    PROSITEiPS00738. ADOHCYASE_1. 1 hit.
    PS00739. ADOHCYASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q80SW1-1 [UniParc]FASTAAdd to Basket

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    MSMPDAMPLP GVGEELKQAK EIEDAEKYSF MATVTKAPKK QIQFADDMQE    50
    FTKFPTKTGR RSLSRSISQS STDSYSSAAS YTDSSDDEVS PREKQQTNSK 100
    GSSNFCVKNI KQAEFGRREI EIAEQDMSAL ISLRKRAQGE KPLAGAKIVG 150
    CTHITAQTAV LIETLCALGA QCRWSACNIY STQNEVAAAL AEAGVAVFAW 200
    KGESEDDFWW CIDRCVNMDG WQANMILDDG GDLTHWVYKK YPNVFKKIRG 250
    IVEESVTGVH RLYQLSKAGK LCVPAMNVND SVTKQKFDNL YCCRESILDG 300
    LKRTTDVMFG GKQVVVCGYG EVGKGCCAAL KALGAIVYIT EIDPICALQA 350
    CMDGFRVVKL NEVIRQVDVV ITCTGNKNVV TREHLDRMKN SCIVCNMGHS 400
    NTEIDVTSLR TPELTWERVR SQVDHVIWPD GKRVVLLAEG RLLNLSCSTV 450
    PTFVLSITAT TQALALIELY NAPEGRYKQD VYLLPKKMDE YVASLHLPSF 500
    DAHLTELTDD QAKYLGLNKN GPFKPNYYRY 530
    Length:530
    Mass (Da):58,951
    Last modified:June 1, 2003 - v1
    Checksum:i974D23361A245D04
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB092504 mRNA. Translation: BAC65166.1.
    BC018218 mRNA. Translation: AAH18218.2.
    BK000547 mRNA. Translation: DAA00059.1.
    CCDSiCCDS38593.1.
    RefSeqiNP_663517.2. NM_145542.3.
    UniGeneiMm.220328.

    Genome annotation databases

    EnsembliENSMUST00000029490; ENSMUSP00000029490; ENSMUSG00000027893.
    GeneIDi229709.
    KEGGimmu:229709.
    UCSCiuc008qxi.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB092504 mRNA. Translation: BAC65166.1 .
    BC018218 mRNA. Translation: AAH18218.2 .
    BK000547 mRNA. Translation: DAA00059.1 .
    CCDSi CCDS38593.1.
    RefSeqi NP_663517.2. NM_145542.3.
    UniGenei Mm.220328.

    3D structure databases

    ProteinModelPortali Q80SW1.
    SMRi Q80SW1. Positions 101-530.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q80SW1. 1 interaction.
    MINTi MINT-4437427.
    STRINGi 10090.ENSMUSP00000029490.

    PTM databases

    PhosphoSitei Q80SW1.

    Proteomic databases

    MaxQBi Q80SW1.
    PaxDbi Q80SW1.
    PRIDEi Q80SW1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000029490 ; ENSMUSP00000029490 ; ENSMUSG00000027893 .
    GeneIDi 229709.
    KEGGi mmu:229709.
    UCSCi uc008qxi.1. mouse.

    Organism-specific databases

    CTDi 10768.
    MGIi MGI:2385184. Ahcyl1.

    Phylogenomic databases

    eggNOGi COG0499.
    GeneTreei ENSGT00390000003626.
    HOGENOMi HOG000227986.
    HOVERGENi HBG005041.
    InParanoidi Q80SW1.
    KOi K01251.
    OMAi KQIQFVE.
    PhylomeDBi Q80SW1.
    TreeFami TF300415.

    Enzyme and pathway databases

    UniPathwayi UPA00314 ; UER00076 .

    Miscellaneous databases

    NextBioi 379631.
    PROi Q80SW1.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q80SW1.
    Bgeei Q80SW1.
    CleanExi MM_AHCYL1.
    Genevestigatori Q80SW1.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR000043. Adenosylhomocysteinase.
    IPR015878. Ado_hCys_hydrolase_NAD-bd.
    IPR016040. NAD(P)-bd_dom.
    IPR020082. S-Ado-L-homoCys_hydrolase_CS.
    [Graphical view ]
    PANTHERi PTHR23420. PTHR23420. 1 hit.
    Pfami PF05221. AdoHcyase. 1 hit.
    PF00670. AdoHcyase_NAD. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001109. Ad_hcy_hydrolase. 1 hit.
    SMARTi SM00996. AdoHcyase. 1 hit.
    SM00997. AdoHcyase_NAD. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00936. ahcY. 1 hit.
    PROSITEi PS00738. ADOHCYASE_1. 1 hit.
    PS00739. ADOHCYASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "IRBIT, a novel inositol 1,4,5-trisphosphate (IP3) receptor-binding protein, is released from the IP3 receptor upon IP3 binding to the receptor."
      Ando H., Mizutani A., Matsu-ura T., Mikoshiba K.
      J. Biol. Chem. 278:10602-10612(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH ITPR1.
      Tissue: Cerebellum.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Czech II.
      Tissue: Mammary tumor.
    3. Cited for: PROTEIN SEQUENCE OF 74-80, INTERACTION WITH ITPR1, MUTAGENESIS OF 70-SER--ASP-73; ASP-73; 82-THR--ASP-88 AND 86-ASP--GLU-88, SUBCELLULAR LOCATION.
    4. Lubec G., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 479-486, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: OF1.
      Tissue: Hippocampus.
    5. "Identification of an S-adenosylhomocysteine hydrolase-like transcript induced during dendritic cell differentiation."
      Dekker J.W., Budhia S., Angel N.Z., Cooper B.J., Clark G.J., Hart D.N., Kato M.
      Immunogenetics 53:993-1001(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    7. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-40, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiSAHH2_MOUSE
    AccessioniPrimary (citable) accession number: Q80SW1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 4, 2006
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3