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Q80RC7

- GCNT3_BHV4

UniProt

Q80RC7 - GCNT3_BHV4

Protein

Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase

Gene

Bo17

Organism
Bovine herpesvirus 4 (BoHV-4) (Movar virus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Non-essential glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans. Contributes to the post-translational modifications of structural proteins.

    Catalytic activityi

    UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R.
    UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.

    Pathwayi

    GO - Molecular functioni

    1. beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC
    2. N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT14. Glycosyltransferase Family 14.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase (EC:2.4.1.102, EC:2.4.1.150)
    Alternative name(s):
    C2GnT-mucin type
    Short name:
    C2GnT-M
    Gene namesi
    Name:Bo17
    OrganismiBovine herpesvirus 4 (BoHV-4) (Movar virus)
    Taxonomic identifieri10385 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeRhadinovirus
    Virus hostiBos taurus (Bovine) [TaxID: 9913]
    Felis catus (Cat) (Felis silvestris catus) [TaxID: 9685]
    Panthera leo (Lion) [TaxID: 9689]

    Subcellular locationi

    GO - Cellular componenti

    1. host cell Golgi membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Host Golgi apparatus, Host membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 439439Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferasePRO_0000288551Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi71 – 711N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi72 ↔ 229By similarity
    Glycosylationi107 – 1071N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi163 ↔ 383By similarity
    Disulfide bondi184 ↔ 211By similarity
    Disulfide bondi392 ↔ 424By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Developmental stagei

    Expressed during BHV-4 repkication (at protein level).

    Structurei

    3D structure databases

    ProteinModelPortaliQ80RC7.
    SMRiQ80RC7. Positions 69-434.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1111CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini30 – 439410LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei12 – 2918Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 14 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Family and domain databases

    InterProiIPR003406. Glyco_trans_14.
    [Graphical view]
    PfamiPF02485. Branch. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q80RC7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVGWKKKKLC RGHHLWVLGC YMLLAVVSLR LSLRFKCDVD SLDLESRDFQ    50
    SQHCRDMLYN NLKLPAKRSI NCSGITRGDQ EAVVQALLDN LEVKKKRPPL 100
    TDTYYLNITR DCERFKAQRK FIQFPLSKEE LDFPIAYSMV VHEKIENFER 150
    LLRAVYAPQN IYCVHVDVKS PETFKEAVKA IISCFPNVFM ASKLVPVVYA 200
    SWSRVQADLN CMEDLLQSSV SWKYLLNTCG TDFPIKTNAE MVLALKMLKG 250
    KNSMESEVPS ESKKNRWKYH YEVTDTLYPT SKMKDPPPDN LPMFTGNAYF 300
    VASRAFVQHV LDNPKSQRLV EWVKDTYSPD EHLWATLQRA PWMPGSVPSH 350
    PKYHISDMTA VARLVKWQYH EGDVSMGAPY APCSGIHRRA ICIYGAGDLY 400
    WILQNHHLLA NKFDPRVDDN VLQCLEEYLR HKAIYGTEL 439
    Length:439
    Mass (Da):50,741
    Last modified:June 1, 2003 - v1
    Checksum:i70E67B83A41A887D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY143158 Genomic DNA. Translation: AAN18278.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY143158 Genomic DNA. Translation: AAN18278.1 .

    3D structure databases

    ProteinModelPortali Q80RC7.
    SMRi Q80RC7. Positions 69-434.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT14. Glycosyltransferase Family 14.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Family and domain databases

    InterProi IPR003406. Glyco_trans_14.
    [Graphical view ]
    Pfami PF02485. Branch. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The core 2 beta-1,6-N-acetylglucosaminyltransferase-mucin encoded by bovine herpesvirus 4 was acquired from an ancestor of the African buffalo."
      Markine-Goriaynoff N., Georgin J.-P., Goltz M., Zimmermann W., Broll H., Wamwayi H.M., Pastoret P.-P., Sharp P.M., Vanderplasschen A.
      J. Virol. 77:1784-1792(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: M40.

    Entry informationi

    Entry nameiGCNT3_BHV4
    AccessioniPrimary (citable) accession number: Q80RC7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2007
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Was acquired from an ancestor of the African buffalo around 1.5 million years ago.

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3