Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q80RC7 (GCNT3_BHV4) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase

EC=2.4.1.102
EC=2.4.1.150
Alternative name(s):
C2GnT-mucin type
Short name=C2GnT-M
Gene names
Name:Bo17
OrganismBovine herpesvirus 4 (BoHV-4) (Movar virus)
Taxonomic identifier10385 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeRhadinovirus
Virus hostBos taurus (Bovine) [TaxID: 9913]
Felis catus (Cat) (Felis silvestris catus) [TaxID: 9685]
Panthera leo (Lion) [TaxID: 9689]

Protein attributes

Sequence length439 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Non-essential glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans. Contributes to the post-translational modifications of structural proteins.

Catalytic activity

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R.

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Host Golgi apparatus membrane; Single-pass type II membrane protein By similarity.

Developmental stage

Expressed during BHV-4 repkication (at protein level).

Miscellaneous

Was acquired from an ancestor of the African buffalo around 1.5 million years ago.

Sequence similarities

Belongs to the glycosyltransferase 14 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 439439Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase
PRO_0000288551

Regions

Topological domain1 – 1111Cytoplasmic Potential
Transmembrane12 – 2918Helical; Signal-anchor for type II membrane protein; Potential
Topological domain30 – 439410Lumenal Potential

Amino acid modifications

Glycosylation711N-linked (GlcNAc...); by host Potential
Glycosylation1071N-linked (GlcNAc...); by host Potential
Disulfide bond72 ↔ 229 By similarity
Disulfide bond163 ↔ 383 By similarity
Disulfide bond184 ↔ 211 By similarity
Disulfide bond392 ↔ 424 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q80RC7 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 70E67B83A41A887D

FASTA43950,741
        10         20         30         40         50         60 
MVGWKKKKLC RGHHLWVLGC YMLLAVVSLR LSLRFKCDVD SLDLESRDFQ SQHCRDMLYN 

        70         80         90        100        110        120 
NLKLPAKRSI NCSGITRGDQ EAVVQALLDN LEVKKKRPPL TDTYYLNITR DCERFKAQRK 

       130        140        150        160        170        180 
FIQFPLSKEE LDFPIAYSMV VHEKIENFER LLRAVYAPQN IYCVHVDVKS PETFKEAVKA 

       190        200        210        220        230        240 
IISCFPNVFM ASKLVPVVYA SWSRVQADLN CMEDLLQSSV SWKYLLNTCG TDFPIKTNAE 

       250        260        270        280        290        300 
MVLALKMLKG KNSMESEVPS ESKKNRWKYH YEVTDTLYPT SKMKDPPPDN LPMFTGNAYF 

       310        320        330        340        350        360 
VASRAFVQHV LDNPKSQRLV EWVKDTYSPD EHLWATLQRA PWMPGSVPSH PKYHISDMTA 

       370        380        390        400        410        420 
VARLVKWQYH EGDVSMGAPY APCSGIHRRA ICIYGAGDLY WILQNHHLLA NKFDPRVDDN 

       430 
VLQCLEEYLR HKAIYGTEL 

« Hide

References

[1]"The core 2 beta-1,6-N-acetylglucosaminyltransferase-mucin encoded by bovine herpesvirus 4 was acquired from an ancestor of the African buffalo."
Markine-Goriaynoff N., Georgin J.-P., Goltz M., Zimmermann W., Broll H., Wamwayi H.M., Pastoret P.-P., Sharp P.M., Vanderplasschen A.
J. Virol. 77:1784-1792(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: M40.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY143158 Genomic DNA. Translation: AAN18278.1.

3D structure databases

ProteinModelPortalQ80RC7.
SMRQ80RC7. Positions 69-434.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGT14. Glycosyltransferase Family 14.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00378.

Family and domain databases

InterProIPR003406. Glyco_trans_14.
[Graphical view]
PfamPF02485. Branch. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGCNT3_BHV4
AccessionPrimary (citable) accession number: Q80RC7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: June 1, 2003
Last modified: February 19, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways