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Protein

Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase

Gene

Bo17

Organism
Bovine herpesvirus 4 (BoHV-4) (Movar virus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Non-essential glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans. Contributes to the post-translational modifications of structural proteins.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R.
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.

Pathway:iprotein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT14. Glycosyltransferase Family 14.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase (EC:2.4.1.102, EC:2.4.1.150)
Alternative name(s):
C2GnT-mucin type
Short name:
C2GnT-M
Gene namesi
Name:Bo17
OrganismiBovine herpesvirus 4 (BoHV-4) (Movar virus)
Taxonomic identifieri10385 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeRhadinovirus
Virus hostiBos taurus (Bovine) [TaxID: 9913]
Felis catus (Cat) (Felis silvestris catus) [TaxID: 9685]
Panthera leo (Lion) [TaxID: 9689]

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1111CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei12 – 2918Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini30 – 439410LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host Golgi apparatus, Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 439439Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferasePRO_0000288551Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi71 – 711N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi72 ↔ 229By similarity
Glycosylationi107 – 1071N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi163 ↔ 383By similarity
Disulfide bondi184 ↔ 211By similarity
Disulfide bondi392 ↔ 424By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Developmental stagei

Expressed during BHV-4 repkication (at protein level).

Structurei

3D structure databases

ProteinModelPortaliQ80RC7.
SMRiQ80RC7. Positions 69-434.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 14 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR003406. Glyco_trans_14.
[Graphical view]
PfamiPF02485. Branch. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q80RC7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVGWKKKKLC RGHHLWVLGC YMLLAVVSLR LSLRFKCDVD SLDLESRDFQ
60 70 80 90 100
SQHCRDMLYN NLKLPAKRSI NCSGITRGDQ EAVVQALLDN LEVKKKRPPL
110 120 130 140 150
TDTYYLNITR DCERFKAQRK FIQFPLSKEE LDFPIAYSMV VHEKIENFER
160 170 180 190 200
LLRAVYAPQN IYCVHVDVKS PETFKEAVKA IISCFPNVFM ASKLVPVVYA
210 220 230 240 250
SWSRVQADLN CMEDLLQSSV SWKYLLNTCG TDFPIKTNAE MVLALKMLKG
260 270 280 290 300
KNSMESEVPS ESKKNRWKYH YEVTDTLYPT SKMKDPPPDN LPMFTGNAYF
310 320 330 340 350
VASRAFVQHV LDNPKSQRLV EWVKDTYSPD EHLWATLQRA PWMPGSVPSH
360 370 380 390 400
PKYHISDMTA VARLVKWQYH EGDVSMGAPY APCSGIHRRA ICIYGAGDLY
410 420 430
WILQNHHLLA NKFDPRVDDN VLQCLEEYLR HKAIYGTEL
Length:439
Mass (Da):50,741
Last modified:June 1, 2003 - v1
Checksum:i70E67B83A41A887D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY143158 Genomic DNA. Translation: AAN18278.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY143158 Genomic DNA. Translation: AAN18278.1.

3D structure databases

ProteinModelPortaliQ80RC7.
SMRiQ80RC7. Positions 69-434.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT14. Glycosyltransferase Family 14.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00378.

Family and domain databases

InterProiIPR003406. Glyco_trans_14.
[Graphical view]
PfamiPF02485. Branch. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The core 2 beta-1,6-N-acetylglucosaminyltransferase-mucin encoded by bovine herpesvirus 4 was acquired from an ancestor of the African buffalo."
    Markine-Goriaynoff N., Georgin J.-P., Goltz M., Zimmermann W., Broll H., Wamwayi H.M., Pastoret P.-P., Sharp P.M., Vanderplasschen A.
    J. Virol. 77:1784-1792(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: M40.

Entry informationi

Entry nameiGCNT3_BHV4
AccessioniPrimary (citable) accession number: Q80RC7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: June 1, 2003
Last modified: January 7, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Was acquired from an ancestor of the African buffalo around 1.5 million years ago.

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.