Reviewed, UniProtKB/Swiss-Prot Q80KJ7 (NS1AB_MASV1)

Names and origin

Protein names

Recommended name:
    Non-structural polyprotein 1AB
Cleaved into the following 5 chains:
    1- Recommended name:
            Protein p19
    2- Recommended name:
            Transmembrane protein 1A
    3- Recommended name:
            Serine protease p27
                Short name=p27
              EC=3.4.21.-
    4- Recommended name:
            Protein p20
    5- Recommended name:
            RNA-directed RNA polymerase p57
                Short name=p57
              EC=2.7.7.48

Gene names

Name:

ORF1

Organism

Mink astrovirus 1 (MAstV-1)

Taxonomic identifier

219688 [NCBI]

Taxonomic lineage

Viruses › ssRNA positive-strand viruses, no DNA stage › Astroviridae › Mamastrovirus

Virus host

Mustela vison (American mink) (Neovison vison) [TaxID: 452646]

Protein attributes

Sequence length	1381 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Non-structural polyprotein 1AB contains the viral protease participating in the cleavage of the polyprotein into functional products. It contains also the activities necessary for replication of genomic RNA, as well as transcription of subgenomic mRNA By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subcellular location	Host membrane; Multi-pass membrane protein Potential.
Post-translational modification	Cleaved presumably by viral and host proteases By similarity.
Sequence similarities	Belongs to the astroviridae polyprotein 1AB family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Cellular component	Membrane
Coding sequence diversity	Ribosomal frameshifting
Domain	Coiled coil Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Nucleotidyltransferase Protease RNA-directed RNA polymerase Thiol protease Transferase
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]

Isoform nsp1ab (identifier: Q80KJ7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Generated by a ribosomal frameshift at position 865.

Isoform nsp1a (identifier: Q80KJ8-1)

The sequence of this isoform can be found in the external entry Q80KJ8-1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1381	1381	Non-structural polyprotein 1AB		PRO_0000327295
	Chain	1 – 195	195	Protein p19 Potential		PRO_0000327296
	Chain	196 – 436	241	Transmembrane protein 1A Potential		PRO_0000327297
	Chain	437 – 662	226	Serine protease p27 Potential		PRO_0000327298
	Chain	663 – 867	205	Protein p20 Potential		PRO_0000327299
	Chain	868 – 1381	514	RNA-directed RNA polymerase p57 Potential		PRO_0000327300
Regions
	Transmembrane	169 – 189	21	Potential
	Transmembrane	249 – 269	21	Potential
	Transmembrane	279 – 299	21	Potential
	Transmembrane	324 – 344	21	Potential
	Transmembrane	365 – 385	21	Potential
	Domain	1122 – 1254	133	RdRp catalytic
	Coiled coil	121 – 160	40	Potential
	Coiled coil	703 – 732	30	Potential
Sites
	Active site	477	1	Charge relay system; for serine protease activity By similarity
	Active site	506	1	Charge relay system; for serine protease activity By similarity
	Active site	569	1	Charge relay system; for serine protease activity By similarity
	Site	195 – 196	2	Cleavage Potential
	Site	436 – 437	2	Cleavage Potential
	Site	662 – 663	2	Cleavage Potential
	Site	867 – 868	2	Cleavage Potential

Sequences

	Sequence		Length	Mass (Da)
	Isoform nsp1ab [UniParc]. Last modified April 8, 2008. Version 2. Checksum: 51B926928D22E51C	FASTA	1,381	156,954
	10 20 30 40 50 60 MANNTTSALH PRGSGQRCVY DTVLRFGDPD ARRRGFQLDE VSHNKLCDIF DSGPLHFAFG 70 80 90 100 110 120 DLKVMKVAGG VVTPHKTVVK TVYVSGVQEG NDYVTFAFTP GPNEWREVDP RIDKRTALVG 130 140 150 160 170 180 VLVQEHKKLD SDLKESRREL SQLKLEHSLL RHDYERLVRE KPGPAMRTFK FSAVIFYAFF 190 200 210 220 230 240 LGFLLMSAVK GEVYGRCLDS ELNLNGNPEV CLHWEEVKSF SLQVALADFW NMTLDYYATV 250 260 270 280 290 300 APQSPLMDLA LGYFPYFANW HMAAFLVGTA HVVAAERPLY MLVTLVLATL SRFQLVALAA 310 320 330 340 350 360 VPMLDMPSSI GLWVTMVLFA IDQAFAILAS VLISVLLLIL CLAMNDVDYG ALLRGCVTLV 370 380 390 400 410 420 SATVFSHLVS FLHAPGWFTI IAILIYRIPK VLSYVSAERV DIKGPDGKIK ETQNANPSWI 430 440 450 460 470 480 TKMSGLKNFF QRAFRQKVRT GVNPTTRIIP NSLVVIDAKD GRGTGFRVRN YLVTAGHVVG 490 500 510 520 530 540 ADTTVRVRWA DVTSFAHVVY RVPNKDIVLL TLPAEYNSLH SYKLAKEVVD GTVVVVSNGD 550 560 570 580 590 600 GGALSVGISE GVIVGESMTY AINTADGMSG SPLTTTDGRL IGVHQQNTGF TGGAVIFRDT 610 620 630 640 650 660 DFPQPKKPQR EADLEAKVAE LEKALAAYTQ SATGEDIVGL VRVAIQREME VLRKELSNEF 670 680 690 700 710 720 GQAKGKTKHK RRIMAAARSG GKRKPGKVWT EEEYKKLLEE GFTRDQLREM AEAAREADDD 730 740 750 760 770 780 FDDYEEEKNE VDYPVWSDHD SDEEIDRDWF GQNLPTWSSA WSDFEPELDP DVTKTLPCHL 790 800 810 820 830 840 EDKFSLKHYI ITEADLKHFG QEMKEYMDHL DAVIKTHTEK GKWCPNTNTE EILKDLNAMW 850 860 870 880 890 900 FKLNHTMWKN GVAPFMQRKK QKPKKREEGP ERGPINPDEM RLDHWEKMMA PPDAGRRLVP 910 920 930 940 950 960 DDYPVIGKLP INRPISDWDE PVDDLLNLLP PAPDSSAYGP AVWGPEAYVK SFEKFTYAKP 970 980 990 1000 1010 1020 RDSIKRDFPR EWKFACQVLR REFDFLEGSV IMDITATSKN ADSTCAYPKC NYWKTEAEYL 1030 1040 1050 1060 1070 1080 SERGYQDYVS EYKRIHGGAR PKVLWLLFLK KEILKVKKIN DSDIRQIVCA DPIFARIGNA 1090 1100 1110 1120 1130 1140 FEEHQNTLMK HRTATRMPQC GWTPFFNGFK RRIERLLSRK NSVFIEFDWT RYDGTIPREI 1150 1160 1170 1180 1190 1200 FAKIKSFRFS CLAEEFQTDA NRAMYQWYCD SLLDRYVLMP SGEVTRQTKG NPSGQISTTM 1210 1220 1230 1240 1250 1260 DNNLCNVFFQ AFEYAYIHPE KSIEELRESW DRCDSLIYGD DRLTTFDHVP PDYVDRVVHM 1270 1280 1290 1300 1310 1320 YKDVFGMWVK PEKVIVSDTP VGLSFCGFTV GPDLMPVPTD CDKLVASLVT PTKKLQDIVA 1330 1340 1350 1360 1370 1380 LYSKVLCYRI LGHNLSDEHE FKRYVRVALE VLARHIRNLG GEEPVHVTER LLDKLWRGGP K
	Isoform nsp1a. See Q80KJ8.	FASTA	–	–

References

[1]	"Molecular characterization of a novel astrovirus associated with disease in mink." Mittelholzer C., Hedlund K.O., Englund L., Dietz H.H., Svensson L. J. Gen. Virol. 84:3087-3094(2003) [PubMed: 14573813] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY179509 Genomic RNA. Translation: AAO32082.1. Sequence problems.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR001730. Peptidase_C4. IPR001254. Peptidase_S1_S6. IPR007094. RNA_pol_PSvir. [Graphical view]
Pfam	PF00863. Peptidase_C4. 1 hit. [Graphical view]
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50507. RDRP_SSRNA_POS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NS1AB_MASV1

Accession

Primary (citable) accession number: Q80KJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	April 8, 2008
Last modified:	June 16, 2009
This is version 31 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)