Q80J95 (Q80J95_9CALI) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names	Submitted name: Polyprotein EMBL AAO63098.2
Organism	Murine norovirus 1 EMBL AAO63098.2
Taxonomic identifier	223997 [NCBI]
Taxonomic lineage	Viruses › ssRNA viruses › ssRNA positive-strand viruses, no DNA stage › Caliciviridae › Norovirus › unclassified Norovirus › Murine norovirus

Protein attributes

Sequence length	1687 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). SAAS SAAS001205
Sequence similarities	Contains 1 RdRp catalytic domain. SAAS SAAS001205 Contains 1 SF3 helicase domain. SAAS SAAS001205

Ontologies

Keywords
Biological process	Viral RNA replication SAAS SAAS001205
Ligand	ATP-binding SAAS SAAS001205 Magnesium PDB 3NAI Metal-binding PDB 3NAI Nucleotide-binding
Molecular function	Hydrolase Nucleotidyltransferase Protease RNA-directed RNA polymerase SAAS SAAS001205 Thiol protease SAAS SAAS001205 Transferase
Technical term	3D-structure PDB 3SFG PDB 3SFU PDB 3UPF PDB 3UQS PDB 3UR0 PDB 4ASH PDB 3NAH PDB 3NAI PDB 3QID PDB 2M4G
Gene Ontology (GO)
Biological_process	interspecies interaction between organisms Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro viral genome replication Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA helicase activity Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	1422	1	Magnesium PDB 3NAI
	Metal binding	1523	1	Magnesium PDB 3NAI

Sequences

	Sequence		Length	Mass (Da)
	Q80J95 [UniParc]. Last modified December 6, 2005. Version 2. Checksum: EA493276296FDB23	FASTA	1,687	187,473
	10 20 30 40 50 60 MRMATPSSAP SVRNTEKRKN KKASSKASVS FGAPSPLSSE SEDEINYMTP PEQEAQPGAL 70 80 90 100 110 120 AALHAEGPLA GLPVTRSDAR VLIFNEWEER KKSDPWLRLD MSDKAIFRRY PHLRPKEDRP 130 140 150 160 170 180 DAPSHAEDAM DAKEPVIGSI LEQDDHKFYH YSVYIGGGLV MGVNNPSAAV CQATIDVEKL 190 200 210 220 230 240 HLWWRPVWEP RXPLDSAELR KCVGMTVPYV ATTVNCYQVC CWIVGIKDTW LKRAKISRDL 250 260 270 280 290 300 PFYSPVQDWN VDPQEPFIPS KLRMVSDGIL VALSAVIGRP IKNLLASVKP LNILNIVLSC 310 320 330 340 350 360 DWTFSGIVNA LILLAELFDI FWTPPDVTNW MISIFGEWQA EGPFDLALDV VPTLLGGIGM 370 380 390 400 410 420 AFGLTSETIG RKLASTNSAL KAAQEMGKFA IEVFKQIMAW IWPSEDPVPA LLSNMEQAII 430 440 450 460 470 480 KNECQLENQL TAMLRDRNAG AEFLRSLDEE EQEVRKIAAK CGNSATTGTT NALLARISMA 490 500 510 520 530 540 RAAFEKARAE QTSRVRPVVI MVSGRPGIGK TCFCQNLAKR IAASLGDETS VGIIPRADVD 550 560 570 580 590 600 HWDAYKGARV VLWDDFGMDN VVKDALRLQM LADTCPVTLN CDRIENKGKM FDSQVIIITT 610 620 630 640 650 660 NQQTPVPLDY VNLEAVCRRI DFLVYAESPV VDDARARAPG DVNAVKAAMR PDYSHINFIL 670 680 690 700 710 720 APQGGFDRQG NTPYGKGVTK IIGATALCAR AVALVHERHD DFGLQNKVYD FDAGKVTAFK 730 740 750 760 770 780 AMAADAGIPW YKMAAIGCKA MGCTCVEEAM HLLKDYEVAP CQVIYNGATY NVSCIKGAPM 790 800 810 820 830 840 VEKVKEPELP KTLVNCVRRI KEARLRCYCR MAADVITSIL QAAGTAFSIY HQIEKRSRPS 850 860 870 880 890 900 FYWDXGYTYR DGPGSFDIFE DDDDGWYHSE GKKGKNKKGR GRPGVFRTRG LTDEEYDEFK 910 920 930 940 950 960 KRRESRGGKY SIDDYLADRE REEELLERDE EEAIFGDGFG LKATRRSRKA ERAKLGLVSG 970 980 990 1000 1010 1020 GDIRARKPID WNVVGPSWAD DDRQVDYGEK INFEAPVSIW SRVVQFGTGW GFWVSGHVFI 1030 1040 1050 1060 1070 1080 TAKHVAPPKG TEIFGRKPGD FTVTSSGDFL KYYFTSAVRP DIPAMVLENG CQEGVVASVL 1090 1100 1110 1120 1130 1140 VKRASGEMLA LAVRMGSQAA IKIGSAVVHG QTGMLLTGSN AKAQDLGTIP GDCGCPYVYK 1150 1160 1170 1180 1190 1200 KGNTWVVIGV HVAATRSGNT VIAATHGEPT LEALEFQGPP MLPRPSGTYA GLPIADYGDA 1210 1220 1230 1240 1250 1260 PPLSTKTMFW RTSPEKLPPG AWEPAYLGSK DERVDGPSLQ QVMRDQLKPY SEPRGLLPPQ 1270 1280 1290 1300 1310 1320 EILDAVCDAI ENRLENTLEP QKPWTFKKAC ESLDKNTSSG YPYHKQKSKD WTGSAFIGDL 1330 1340 1350 1360 1370 1380 GDQATHANNM YEMGKSMRPI YTAALKDELV KPDKIYGKIK KRLLWGSDLG TMIRAARAFG 1390 1400 1410 1420 1430 1440 PFCDALKETC IFNPIRVGMS MNEDGPFIFA RHANFRYHMD ADYTRWDSTQ QRAILKRAGD 1450 1460 1470 1480 1490 1500 IMVRLSPEPD LARVVMDDLL APSLLDVGDY KIVVEEGLPS GCPCTTQLNS LAHWILTLCA 1510 1520 1530 1540 1550 1560 MVEVTRVDPD IVMQESEFSF YGDDEVVSTN LELDMVKYTM ALRRYGLLPT RADKEEGPLE 1570 1580 1590 1600 1610 1620 RRQTLQGISF LRRAIVGDQF GWYGRLDRAS IDRQLLWTKG PNHQNPFETL PGHAQRPSQL 1630 1640 1650 1660 1670 1680 MALLGEAAMH GEKYYRTVAS RVSKEAAQSG IEMVVPRHRS VLRWVRFGTM DAETPQERSA VFVNEDE

References

[1]	"STAT1-dependent innate immunity to a Norwalk-like virus." Karst S.M., Wobus C.E., Lay M., Davidson J., Virgin H.W. Science 299:1575-1578(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: Mu/NoV/GV/MNV1/2002/USA EMBL AAO63098.2.
[2]	"Development of a microsphere-based serologic multiplexed fluorescent immunoassay and a reverse transcriptase PCR assay to detect murine norovirus 1 infection in mice." Hsu C.C., Wobus C.E., Steffen E.K., Riley L.K., Livingston R.S. Clin. Diagn. Lab. Immunol. 12:1145-1151(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: Mu/NoV/GV/MNV1/2002/USA EMBL AAO63098.2.
[3]	Karst S.M., Wobus C.E., Lay M., Davidson J., Virgin H.W.IV. Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: Mu/NoV/GV/MNV1/2002/USA EMBL AAO63098.2.
[4]	"Crystal structures and functional analysis of murine norovirus RNA-dependent RNA polymerase." Kim K.H., Lee J.H., Alam I., Park Y., Kang S. Submitted (JUN-2010) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.75 ANGSTROMS) OF 1181-1686.
[5]	"Crystal structures of murine norovirus-1 RNA-dependent RNA polymerase." Lee J.H., Alam I., Han K.R., Cho S., Shin S., Kang S., Yang J.M., Kim K.H. J. Gen. Virol. 92:1607-1616(2011) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 1181-1686.
[6]	"Structure-based inhibition of Norovirus RNA-dependent RNA polymerases." Mastrangelo E., Pezzullo M., Tarantino D., Petazzi R., Germani F., Kramer D., Robel I., Rohayem J., Bolognesi M., Milani M. J. Mol. Biol. 419:198-210(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 1181-1687.
[7]	"Structure of a murine norovirus NS6 protease-product complex revealed by adventitious crystallisation." Leen E.N., Baeza G., Curry S. PLoS ONE 7:e38723-e38723(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.58 ANGSTROMS) OF 995-1177.
[8]	"Crystal structures of murine norovirus-1 RNA-dependent RNA polymerase in complex with 2-thiouridine or ribavirin." Alam I., Lee J.H., Cho K.J., Han K.R., Yang J.M., Chung M.S., Kim K.H. Virology 426:143-151(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.21 ANGSTROMS) OF 1181-1686.
[9]	"Structures of the Compact Helical Core Domains of Feline Calicivirus and Murine Norovirus VPg proteins." Leen E.N., Kwok K.Y., Birtley J.R., Simpson P.J., Subba-Reddy C.V., Chaudhry Y., Sosnovtsev S.V., Green K.Y., Prater S.N., Young J.C., Chung L.M., Marchant J., Roberts L.O., Kao C.C., Matthews S., Goodfellow I.G., Curry S. J. Virol. 0:0-0(2013) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 881-955.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY228235 Genomic RNA. Translation: AAO63098.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2M4G	NMR	-	A	881-955	[»]
3NAH	X-ray	2.75	A/B/C	1181-1686	[»]
3NAI	X-ray	2.56	A/B/C	1181-1686	[»]
3QID	X-ray	2.50	A/B/C	1181-1686	[»]
3SFG	X-ray	2.21	A/B/C	1181-1686	[»]
3SFU	X-ray	2.50	A/B/C	1181-1686	[»]
3UPF	X-ray	2.60	A/B/C	1173-1687	[»]
3UQS	X-ray	2.00	A/B/C	1181-1687	[»]
3UR0	X-ray	2.45	A/B/C	1181-1687	[»]
4ASH	X-ray	1.58	A/B	995-1177	[»]

ProteinModelPortal

Q80J95.

SMR

Q80J95. Positions 995-1167, 1187-1686.

ModBase

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Protein family/group databases

MEROPS

C37.003.

Protocols and materials databases

StructuralBiologyKnowledgebase

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Family and domain databases

InterPro

IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR001665. Norovirus_pept_C37.
IPR027417. P-loop_NTPase.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
IPR013614. Viral_PP_Calicivir_N.
[Graphical view]

Pfam

PF08405. Calici_PP_N. 1 hit.
PF05416. Peptidase_C37. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view]

PRINTS

PR00917. SRSVCYSPTASE.

SUPFAM

SSF50494. Pept_Ser_Cys. 1 hit.
SSF52540. SSF52540. 2 hits.

PROSITE

PS51537. NV_3CL_PRO. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]

ProtoNet

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Other

EvolutionaryTrace

Q80J95.

Entry information

Entry name

Q80J95_9CALI

Accession

Primary (citable) accession number: Q80J95

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 2003
Last sequence update:	December 6, 2005
Last modified:	May 29, 2013
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)