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Q80J95 (Q80J95_9CALI) Unreviewed, UniProtKB/TrEMBL

Last modified April 16, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein attributes

Sequence length1687 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). SAAS SAAS014759

Sequence similarities

Contains RdRp catalytic domain. SAAS SAAS014759

Contains SF3 helicase domain. SAAS SAAS014759

Contains SFhelicase domain. SAAS SAAS014759

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Region1259 – 12602Sulfate 2 binding
Region1259 – 12602Sulfate 8 binding PDB 3NAI
Region1260 – 12612Sulfate 9 binding PDB 3SFG
Region1267 – 12715Sulfate 10 binding PDB 3SFG
Region1326 – 13294Sulfate 14 binding PDB 3SFG PDB 3SFU
Region1326 – 13294Sulfate 15 binding
Region1346 – 13472Sulfate 2 binding PDB 3SFG
Region1587 – 15882Sulfate 27 binding PDB 3NAH

Sites

Metal binding14201Magnesium 1 PDB 3SFG PDB 3SFU
Metal binding14221Magnesium
Metal binding14221Magnesium 2 PDB 3NAI
Metal binding15231Magnesium
Metal binding15231Magnesium 2 PDB 3NAI
Metal binding15241Magnesium 1 PDB 3SFG PDB 3SFU
Metal binding15251Magnesium 1; via carbonyl oxygen PDB 3SFG PDB 3SFU
Metal binding15691Magnesium 1; via carbonyl oxygen PDB 3SFG PDB 3SFU
Binding site12221Sulfate 3 PDB 3UR0
Binding site12291Sulfate 5 PDB 3NAH PDB 3NAI
Binding site12381Sulfate 6
Binding site12381Sulfate 7 PDB 3SFU
Binding site12391Sulfate 5; via amide nitrogen PDB 3NAH PDB 3NAI
Binding site12441Sulfate 6
Binding site12441Sulfate 7 PDB 3SFU
Binding site12981Sulfate 12; via amide nitrogen
Binding site13071Sulfate 13 PDB 3NAI
Binding site13071Sulfate 3
Binding site13361Sulfate 16; via amide nitrogen PDB 3SFU
Binding site13511Sulfate 4 PDB 3UR0
Binding site14161Sulfate 19
Binding site14251Sulfate 21; via amide nitrogen
Binding site14251Sulfate 22; via amide nitrogen PDB 3SFG PDB 3UPF
Binding site14371Sulfate 10 PDB 3SFG
Binding site15151Sulfate 23
Binding site15511Sulfate 24 PDB 3SFG
Binding site15621Sulfate 24 PDB 3SFG
Binding site15631Sulfate 25; via amide nitrogen PDB 3NAI
Binding site15631Sulfate 5; via amide nitrogen
Binding site15651Sulfate 20; via amide nitrogen PDB 3SFU
Binding site15821Sulfate 17 PDB 3NAI
Binding site15821Sulfate 18
Binding site15821Sulfate 4
Binding site15851Sulfate 26 PDB 3SFU PDB 3NAH
Binding site15881Sulfate 28; via amide nitrogen PDB 3SFG
Binding site16041Sulfate 1; via amide nitrogen PDB 3SFU
Binding site16081Sulfate 1 PDB 3SFU
Binding site16301Sulfate 27 PDB 3UQS
Binding site16631Sulfate 11 PDB 3SFG

Sequences

Sequence LengthMass (Da)Tools
Q80J95 [UniParc].

Last modified December 6, 2005. Version 2.
Checksum: EA493276296FDB23

FASTA1,687187,473
        10         20         30         40         50         60 
MRMATPSSAP SVRNTEKRKN KKASSKASVS FGAPSPLSSE SEDEINYMTP PEQEAQPGAL 

        70         80         90        100        110        120 
AALHAEGPLA GLPVTRSDAR VLIFNEWEER KKSDPWLRLD MSDKAIFRRY PHLRPKEDRP 

       130        140        150        160        170        180 
DAPSHAEDAM DAKEPVIGSI LEQDDHKFYH YSVYIGGGLV MGVNNPSAAV CQATIDVEKL 

       190        200        210        220        230        240 
HLWWRPVWEP RXPLDSAELR KCVGMTVPYV ATTVNCYQVC CWIVGIKDTW LKRAKISRDL 

       250        260        270        280        290        300 
PFYSPVQDWN VDPQEPFIPS KLRMVSDGIL VALSAVIGRP IKNLLASVKP LNILNIVLSC 

       310        320        330        340        350        360 
DWTFSGIVNA LILLAELFDI FWTPPDVTNW MISIFGEWQA EGPFDLALDV VPTLLGGIGM 

       370        380        390        400        410        420 
AFGLTSETIG RKLASTNSAL KAAQEMGKFA IEVFKQIMAW IWPSEDPVPA LLSNMEQAII 

       430        440        450        460        470        480 
KNECQLENQL TAMLRDRNAG AEFLRSLDEE EQEVRKIAAK CGNSATTGTT NALLARISMA 

       490        500        510        520        530        540 
RAAFEKARAE QTSRVRPVVI MVSGRPGIGK TCFCQNLAKR IAASLGDETS VGIIPRADVD 

       550        560        570        580        590        600 
HWDAYKGARV VLWDDFGMDN VVKDALRLQM LADTCPVTLN CDRIENKGKM FDSQVIIITT 

       610        620        630        640        650        660 
NQQTPVPLDY VNLEAVCRRI DFLVYAESPV VDDARARAPG DVNAVKAAMR PDYSHINFIL 

       670        680        690        700        710        720 
APQGGFDRQG NTPYGKGVTK IIGATALCAR AVALVHERHD DFGLQNKVYD FDAGKVTAFK 

       730        740        750        760        770        780 
AMAADAGIPW YKMAAIGCKA MGCTCVEEAM HLLKDYEVAP CQVIYNGATY NVSCIKGAPM 

       790        800        810        820        830        840 
VEKVKEPELP KTLVNCVRRI KEARLRCYCR MAADVITSIL QAAGTAFSIY HQIEKRSRPS 

       850        860        870        880        890        900 
FYWDXGYTYR DGPGSFDIFE DDDDGWYHSE GKKGKNKKGR GRPGVFRTRG LTDEEYDEFK 

       910        920        930        940        950        960 
KRRESRGGKY SIDDYLADRE REEELLERDE EEAIFGDGFG LKATRRSRKA ERAKLGLVSG 

       970        980        990       1000       1010       1020 
GDIRARKPID WNVVGPSWAD DDRQVDYGEK INFEAPVSIW SRVVQFGTGW GFWVSGHVFI 

      1030       1040       1050       1060       1070       1080 
TAKHVAPPKG TEIFGRKPGD FTVTSSGDFL KYYFTSAVRP DIPAMVLENG CQEGVVASVL 

      1090       1100       1110       1120       1130       1140 
VKRASGEMLA LAVRMGSQAA IKIGSAVVHG QTGMLLTGSN AKAQDLGTIP GDCGCPYVYK 

      1150       1160       1170       1180       1190       1200 
KGNTWVVIGV HVAATRSGNT VIAATHGEPT LEALEFQGPP MLPRPSGTYA GLPIADYGDA 

      1210       1220       1230       1240       1250       1260 
PPLSTKTMFW RTSPEKLPPG AWEPAYLGSK DERVDGPSLQ QVMRDQLKPY SEPRGLLPPQ 

      1270       1280       1290       1300       1310       1320 
EILDAVCDAI ENRLENTLEP QKPWTFKKAC ESLDKNTSSG YPYHKQKSKD WTGSAFIGDL 

      1330       1340       1350       1360       1370       1380 
GDQATHANNM YEMGKSMRPI YTAALKDELV KPDKIYGKIK KRLLWGSDLG TMIRAARAFG 

      1390       1400       1410       1420       1430       1440 
PFCDALKETC IFNPIRVGMS MNEDGPFIFA RHANFRYHMD ADYTRWDSTQ QRAILKRAGD 

      1450       1460       1470       1480       1490       1500 
IMVRLSPEPD LARVVMDDLL APSLLDVGDY KIVVEEGLPS GCPCTTQLNS LAHWILTLCA 

      1510       1520       1530       1540       1550       1560 
MVEVTRVDPD IVMQESEFSF YGDDEVVSTN LELDMVKYTM ALRRYGLLPT RADKEEGPLE 

      1570       1580       1590       1600       1610       1620 
RRQTLQGISF LRRAIVGDQF GWYGRLDRAS IDRQLLWTKG PNHQNPFETL PGHAQRPSQL 

      1630       1640       1650       1660       1670       1680 
MALLGEAAMH GEKYYRTVAS RVSKEAAQSG IEMVVPRHRS VLRWVRFGTM DAETPQERSA 


VFVNEDE 

« Hide

References

[1]"STAT1-dependent innate immunity to a Norwalk-like virus."
Karst S.M., Wobus C.E., Lay M., Davidson J., Virgin H.W.
Science 299:1575-1578(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: Mu/NoV/GV/MNV1/2002/USA EMBL AAO63098.2.
[2]"Development of a microsphere-based serologic multiplexed fluorescent immunoassay and a reverse transcriptase PCR assay to detect murine norovirus 1 infection in mice."
Hsu C.C., Wobus C.E., Steffen E.K., Riley L.K., Livingston R.S.
Clin. Diagn. Lab. Immunol. 12:1145-1151(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: Mu/NoV/GV/MNV1/2002/USA EMBL AAO63098.2.
[3]Karst S.M., Wobus C.E., Lay M., Davidson J., Virgin H.W.IV.
Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: Mu/NoV/GV/MNV1/2002/USA EMBL AAO63098.2.
[4]"Crystal structures and functional analysis of murine norovirus RNA-dependent RNA polymerase."
Kim K.H., Lee J.H., Alam I., Park Y., Kang S.
Submitted (JUN-2010) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.75 ANGSTROMS) OF 1181-1686 IN COMPLEX WITH SULFATE.
[5]"Crystal structures of murine norovirus-1 RNA-dependent RNA polymerase."
Lee J.H., Alam I., Han K.R., Cho S., Shin S., Kang S., Yang J.M., Kim K.H.
J. Gen. Virol. 92:1607-1616(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 1181-1686 IN COMPLEX WITH SULFATE.
[6]"Structure-based inhibition of Norovirus RNA-dependent RNA polymerases."
Mastrangelo E., Pezzullo M., Tarantino D., Petazzi R., Germani F., Kramer D., Robel I., Rohayem J., Bolognesi M., Milani M.
J. Mol. Biol. 419:198-210(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 1181-1687 IN COMPLEX WITH SULFATE.
[7]"Structure of a murine norovirus NS6 protease-product complex revealed by adventitious crystallisation."
Leen E.N., Baeza G., Curry S.
PLoS ONE 7:e38723-e38723(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.58 ANGSTROMS) OF 995-1177.
[8]"Crystal structures of murine norovirus-1 RNA-dependent RNA polymerase in complex with 2-thiouridine or ribavirin."
Alam I., Lee J.H., Cho K.J., Han K.R., Yang J.M., Chung M.S., Kim K.H.
Virology 426:143-151(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.21 ANGSTROMS) OF 1181-1686 IN COMPLEX WITH MAGNESIUM AND SULFATE.
[9]"Structures of the compact helical core domains of feline calicivirus and murine norovirus VPg proteins."
Leen E.N., Kwok K.Y., Birtley J.R., Simpson P.J., Subba-Reddy C.V., Chaudhry Y., Sosnovtsev S.V., Green K.Y., Prater S.N., Tong M., Young J.C., Chung L.M., Marchant J., Roberts L.O., Kao C.C., Matthews S., Goodfellow I.G., Curry S.
J. Virol. 87:5318-5330(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 881-955.
[10]"Murine norovirus protein NS1/2 aspartate to glutamate mutation sufficient for persistence reorients sidechain of surface exposed tryptophan within a novel structured domain."
Borin B.N., Tang W., Nice T.J., McCune B.T., Virgin H.W., Krezel A.M.
Proteins 0:0-0(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 28-114.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY228235 Genomic RNA. Translation: AAO63098.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2M4GNMR-A881-955[»]
2MCDNMR-A28-114[»]
2MCHNMR-A58-114[»]
3NAHX-ray2.75A/B/C1181-1686[»]
3NAIX-ray2.56A/B/C1181-1686[»]
3QIDX-ray2.50A/B/C1181-1686[»]
3SFGX-ray2.21A/B/C1181-1686[»]
3SFUX-ray2.50A/B/C1181-1686[»]
3UPFX-ray2.60A/B/C1174-1687[»]
3UQSX-ray2.00A/B/C1181-1687[»]
3UR0X-ray2.45A/B/C1181-1687[»]
4ASHX-ray1.58A/B995-1177[»]
ProteinModelPortalQ80J95.
SMRQ80J95. Positions 995-1167, 1187-1686.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC37.003.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR001665. Norovirus_pept_C37.
IPR027417. P-loop_NTPase.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
IPR013614. Viral_PP_Calicivir_N.
[Graphical view]
PfamPF08405. Calici_PP_N. 1 hit.
PF05416. Peptidase_C37. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view]
PRINTSPR00917. SRSVCYSPTASE.
SUPFAMSSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEPS51537. NV_3CL_PRO. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ80J95.

Entry information

Entry nameQ80J95_9CALI
AccessionPrimary (citable) accession number: Q80J95
Entry history
Integrated into UniProtKB/TrEMBL: June 1, 2003
Last sequence update: December 6, 2005
Last modified: April 16, 2014
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)