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Q80J95

- Q80J95_9CALI

UniProt

Q80J95 - Q80J95_9CALI

Protein
Submitted name:

Polyprotein

Gene
N/A
Organism
Murine norovirus 1
Status
Unreviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 2 (06 Dec 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).SAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei1238 – 12381Sulfate 6
    Binding sitei1244 – 12441Sulfate 6
    Binding sitei1298 – 12981Sulfate 12; via amide nitrogen
    Binding sitei1307 – 13071Sulfate 3
    Binding sitei1416 – 14161Sulfate 19
    Metal bindingi1420 – 14201Magnesium 1
    Metal bindingi1422 – 14221Magnesium
    Metal bindingi1422 – 14221Magnesium 2Imported
    Binding sitei1425 – 14251Sulfate 21; via amide nitrogen
    Binding sitei1515 – 15151Sulfate 23
    Metal bindingi1523 – 15231Magnesium
    Metal bindingi1523 – 15231Magnesium 2Imported
    Metal bindingi1524 – 15241Magnesium 1
    Metal bindingi1525 – 15251Magnesium 1; via carbonyl oxygen
    Binding sitei1563 – 15631Sulfate 5; via amide nitrogen
    Metal bindingi1569 – 15691Magnesium 1; via carbonyl oxygen
    Binding sitei1582 – 15821Sulfate 18
    Binding sitei1582 – 15821Sulfate 4

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cysteine-type endopeptidase activity Source: InterPro
    3. metal ion binding Source: UniProtKB-KW
    4. RNA binding Source: InterPro
    5. RNA-directed RNA polymerase activity Source: UniProtKB-KW
    6. RNA helicase activity Source: InterPro

    GO - Biological processi

    1. transcription, DNA-templated Source: InterPro
    2. viral RNA genome replication Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymeraseSAAS annotation, Thiol proteaseSAAS annotation, Transferase

    Keywords - Biological processi

    Viral RNA replicationSAAS annotation

    Keywords - Ligandi

    ATP-bindingSAAS annotation, MagnesiumImported, Metal-bindingImported, Nucleotide-binding

    Protein family/group databases

    MEROPSiC37.003.

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    PolyproteinImported
    OrganismiMurine norovirus 1Imported
    Taxonomic identifieri223997 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageCaliciviridaeNorovirus

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2M4GNMR-A881-955[»]
    2MCDNMR-A28-114[»]
    2MCHNMR-A58-114[»]
    3NAHX-ray2.75A/B/C1181-1686[»]
    3NAIX-ray2.56A/B/C1181-1686[»]
    3QIDX-ray2.50A/B/C1181-1686[»]
    3SFGX-ray2.21A/B/C1181-1686[»]
    3SFUX-ray2.50A/B/C1181-1686[»]
    3UPFX-ray2.60A/B/C1174-1687[»]
    3UQSX-ray2.00A/B/C1181-1687[»]
    3UR0X-ray2.45A/B/C1181-1687[»]
    4ASHX-ray1.58A/B995-1177[»]
    ProteinModelPortaliQ80J95.
    SMRiQ80J95. Positions 995-1167, 1187-1686.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ80J95.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1259 – 12602Sulfate 2 binding
    Regioni1326 – 13294Sulfate 15 binding

    Sequence similaritiesi

    Contains RdRp catalytic domain.SAAS annotation
    Contains SF3 helicase domain.SAAS annotation
    Contains SFhelicase domain.SAAS annotation

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR014759. Helicase_SF3_ssRNA_vir.
    IPR001665. Norovirus_pept_C37.
    IPR027417. P-loop_NTPase.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    IPR013614. Viral_PP_Calicivir_N.
    [Graphical view]
    PfamiPF08405. Calici_PP_N. 1 hit.
    PF05416. Peptidase_C37. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF00910. RNA_helicase. 1 hit.
    [Graphical view]
    PRINTSiPR00917. SRSVCYSPTASE.
    SUPFAMiSSF50494. SSF50494. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEiPS51537. NV_3CL_PRO. 1 hit.
    PS50507. RDRP_SSRNA_POS. 1 hit.
    PS51218. SF3_HELICASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q80J95-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRMATPSSAP SVRNTEKRKN KKASSKASVS FGAPSPLSSE SEDEINYMTP     50
    PEQEAQPGAL AALHAEGPLA GLPVTRSDAR VLIFNEWEER KKSDPWLRLD 100
    MSDKAIFRRY PHLRPKEDRP DAPSHAEDAM DAKEPVIGSI LEQDDHKFYH 150
    YSVYIGGGLV MGVNNPSAAV CQATIDVEKL HLWWRPVWEP RXPLDSAELR 200
    KCVGMTVPYV ATTVNCYQVC CWIVGIKDTW LKRAKISRDL PFYSPVQDWN 250
    VDPQEPFIPS KLRMVSDGIL VALSAVIGRP IKNLLASVKP LNILNIVLSC 300
    DWTFSGIVNA LILLAELFDI FWTPPDVTNW MISIFGEWQA EGPFDLALDV 350
    VPTLLGGIGM AFGLTSETIG RKLASTNSAL KAAQEMGKFA IEVFKQIMAW 400
    IWPSEDPVPA LLSNMEQAII KNECQLENQL TAMLRDRNAG AEFLRSLDEE 450
    EQEVRKIAAK CGNSATTGTT NALLARISMA RAAFEKARAE QTSRVRPVVI 500
    MVSGRPGIGK TCFCQNLAKR IAASLGDETS VGIIPRADVD HWDAYKGARV 550
    VLWDDFGMDN VVKDALRLQM LADTCPVTLN CDRIENKGKM FDSQVIIITT 600
    NQQTPVPLDY VNLEAVCRRI DFLVYAESPV VDDARARAPG DVNAVKAAMR 650
    PDYSHINFIL APQGGFDRQG NTPYGKGVTK IIGATALCAR AVALVHERHD 700
    DFGLQNKVYD FDAGKVTAFK AMAADAGIPW YKMAAIGCKA MGCTCVEEAM 750
    HLLKDYEVAP CQVIYNGATY NVSCIKGAPM VEKVKEPELP KTLVNCVRRI 800
    KEARLRCYCR MAADVITSIL QAAGTAFSIY HQIEKRSRPS FYWDXGYTYR 850
    DGPGSFDIFE DDDDGWYHSE GKKGKNKKGR GRPGVFRTRG LTDEEYDEFK 900
    KRRESRGGKY SIDDYLADRE REEELLERDE EEAIFGDGFG LKATRRSRKA 950
    ERAKLGLVSG GDIRARKPID WNVVGPSWAD DDRQVDYGEK INFEAPVSIW 1000
    SRVVQFGTGW GFWVSGHVFI TAKHVAPPKG TEIFGRKPGD FTVTSSGDFL 1050
    KYYFTSAVRP DIPAMVLENG CQEGVVASVL VKRASGEMLA LAVRMGSQAA 1100
    IKIGSAVVHG QTGMLLTGSN AKAQDLGTIP GDCGCPYVYK KGNTWVVIGV 1150
    HVAATRSGNT VIAATHGEPT LEALEFQGPP MLPRPSGTYA GLPIADYGDA 1200
    PPLSTKTMFW RTSPEKLPPG AWEPAYLGSK DERVDGPSLQ QVMRDQLKPY 1250
    SEPRGLLPPQ EILDAVCDAI ENRLENTLEP QKPWTFKKAC ESLDKNTSSG 1300
    YPYHKQKSKD WTGSAFIGDL GDQATHANNM YEMGKSMRPI YTAALKDELV 1350
    KPDKIYGKIK KRLLWGSDLG TMIRAARAFG PFCDALKETC IFNPIRVGMS 1400
    MNEDGPFIFA RHANFRYHMD ADYTRWDSTQ QRAILKRAGD IMVRLSPEPD 1450
    LARVVMDDLL APSLLDVGDY KIVVEEGLPS GCPCTTQLNS LAHWILTLCA 1500
    MVEVTRVDPD IVMQESEFSF YGDDEVVSTN LELDMVKYTM ALRRYGLLPT 1550
    RADKEEGPLE RRQTLQGISF LRRAIVGDQF GWYGRLDRAS IDRQLLWTKG 1600
    PNHQNPFETL PGHAQRPSQL MALLGEAAMH GEKYYRTVAS RVSKEAAQSG 1650
    IEMVVPRHRS VLRWVRFGTM DAETPQERSA VFVNEDE 1687
    Length:1,687
    Mass (Da):187,473
    Last modified:December 6, 2005 - v2
    Checksum:iEA493276296FDB23
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY228235 Genomic RNA. Translation: AAO63098.2.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY228235 Genomic RNA. Translation: AAO63098.2 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2M4G NMR - A 881-955 [» ]
    2MCD NMR - A 28-114 [» ]
    2MCH NMR - A 58-114 [» ]
    3NAH X-ray 2.75 A/B/C 1181-1686 [» ]
    3NAI X-ray 2.56 A/B/C 1181-1686 [» ]
    3QID X-ray 2.50 A/B/C 1181-1686 [» ]
    3SFG X-ray 2.21 A/B/C 1181-1686 [» ]
    3SFU X-ray 2.50 A/B/C 1181-1686 [» ]
    3UPF X-ray 2.60 A/B/C 1174-1687 [» ]
    3UQS X-ray 2.00 A/B/C 1181-1687 [» ]
    3UR0 X-ray 2.45 A/B/C 1181-1687 [» ]
    4ASH X-ray 1.58 A/B 995-1177 [» ]
    ProteinModelPortali Q80J95.
    SMRi Q80J95. Positions 995-1167, 1187-1686.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C37.003.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q80J95.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR014759. Helicase_SF3_ssRNA_vir.
    IPR001665. Norovirus_pept_C37.
    IPR027417. P-loop_NTPase.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    IPR013614. Viral_PP_Calicivir_N.
    [Graphical view ]
    Pfami PF08405. Calici_PP_N. 1 hit.
    PF05416. Peptidase_C37. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF00910. RNA_helicase. 1 hit.
    [Graphical view ]
    PRINTSi PR00917. SRSVCYSPTASE.
    SUPFAMi SSF50494. SSF50494. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEi PS51537. NV_3CL_PRO. 1 hit.
    PS50507. RDRP_SSRNA_POS. 1 hit.
    PS51218. SF3_HELICASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "STAT1-dependent innate immunity to a Norwalk-like virus."
      Karst S.M., Wobus C.E., Lay M., Davidson J., Virgin H.W.
      Science 299:1575-1578(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: Mu/NoV/GV/MNV1/2002/USAImported.
    2. "Development of a microsphere-based serologic multiplexed fluorescent immunoassay and a reverse transcriptase PCR assay to detect murine norovirus 1 infection in mice."
      Hsu C.C., Wobus C.E., Steffen E.K., Riley L.K., Livingston R.S.
      Clin. Diagn. Lab. Immunol. 12:1145-1151(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: Mu/NoV/GV/MNV1/2002/USAImported.
    3. Karst S.M., Wobus C.E., Lay M., Davidson J., Virgin H.W.IV.
      Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: Mu/NoV/GV/MNV1/2002/USAImported.
    4. "Crystal structures and functional analysis of murine norovirus RNA-dependent RNA polymerase."
      Kim K.H., Lee J.H., Alam I., Park Y., Kang S.
      Submitted (JUN-2010) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.75 ANGSTROMS) OF 1181-1686.
    5. "Crystal structures of murine norovirus-1 RNA-dependent RNA polymerase."
      Lee J.H., Alam I., Han K.R., Cho S., Shin S., Kang S., Yang J.M., Kim K.H.
      J. Gen. Virol. 92:1607-1616(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 1181-1686.
    6. Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 1181-1687.
    7. "Structure of a murine norovirus NS6 protease-product complex revealed by adventitious crystallisation."
      Leen E.N., Baeza G., Curry S.
      PLoS ONE 7:e38723-e38723(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.58 ANGSTROMS) OF 995-1177.
    8. "Crystal structures of murine norovirus-1 RNA-dependent RNA polymerase in complex with 2-thiouridine or ribavirin."
      Alam I., Lee J.H., Cho K.J., Han K.R., Yang J.M., Chung M.S., Kim K.H.
      Virology 426:143-151(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.21 ANGSTROMS) OF 1181-1686 IN COMPLEX WITH MAGNESIUM.
    9. "Structures of the compact helical core domains of feline calicivirus and murine norovirus VPg proteins."
      Leen E.N., Kwok K.Y., Birtley J.R., Simpson P.J., Subba-Reddy C.V., Chaudhry Y., Sosnovtsev S.V., Green K.Y., Prater S.N., Tong M., Young J.C., Chung L.M., Marchant J., Roberts L.O., Kao C.C., Matthews S., Goodfellow I.G., Curry S.
      J. Virol. 87:5318-5330(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 881-955.
    10. "Murine norovirus protein NS1/2 aspartate to glutamate mutation, sufficient for persistence, reorients side chain of surface exposed tryptophan within a novel structured domain."
      Borin B.N., Tang W., Nice T.J., McCune B.T., Virgin H.W., Krezel A.M.
      Proteins 82:1200-1209(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 28-114.

    Entry informationi

    Entry nameiQ80J95_9CALI
    AccessioniPrimary (citable) accession number: Q80J95
    Entry historyi
    Integrated into UniProtKB/TrEMBL: June 1, 2003
    Last sequence update: December 6, 2005
    Last modified: October 1, 2014
    This is version 67 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3