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Protein
Submitted name:

Polyprotein

Gene
N/A
Organism
Murine norovirus 1
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).SAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi1420 – 14201Magnesium 1Combined sources
Metal bindingi1422 – 14221Magnesium 2
Metal bindingi1523 – 15231Magnesium 2
Metal bindingi1524 – 15241Magnesium 1Combined sources
Metal bindingi1525 – 15251Magnesium 1; via carbonyl oxygenCombined sources
Metal bindingi1569 – 15691Magnesium 1; via carbonyl oxygenCombined sources

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cysteine-type endopeptidase activity Source: InterPro
  3. metal ion binding Source: UniProtKB-KW
  4. RNA binding Source: InterPro
  5. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  6. RNA helicase activity Source: InterPro

GO - Biological processi

  1. transcription, DNA-templated Source: InterPro
  2. viral RNA genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymeraseSAAS annotation, Thiol proteaseSAAS annotation, Transferase

Keywords - Biological processi

Viral RNA replicationSAAS annotation

Keywords - Ligandi

ATP-bindingSAAS annotation, MagnesiumCombined sources, Metal-bindingCombined sources, Nucleotide-binding

Protein family/group databases

MEROPSiC37.003.

Names & Taxonomyi

Protein namesi
Submitted name:
PolyproteinImported
OrganismiMurine norovirus 1Imported
Taxonomic identifieri223997 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageCaliciviridaeNorovirus

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi1383 ↔ 1484Combined sources

Keywords - PTMi

PhosphoproteinSAAS annotation

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2M4GNMR-A881-955[»]
2MCDNMR-A28-114[»]
2MCHNMR-A58-114[»]
3NAHX-ray2.75A/B/C1181-1686[»]
3NAIX-ray2.56A/B/C1181-1686[»]
3QIDX-ray2.50A/B/C1181-1686[»]
3SFGX-ray2.21A/B/C1181-1686[»]
3SFUX-ray2.50A/B/C1181-1686[»]
3UPFX-ray2.60A/B/C1174-1687[»]
3UQSX-ray2.00A/B/C1181-1687[»]
3UR0X-ray2.45A/B/C1181-1687[»]
4ASHX-ray1.58A/B995-1177[»]
4NRUX-ray2.30A/B/C/D/E/F1181-1687[»]
4O4RX-ray2.40A/B/C1181-1687[»]
ProteinModelPortaliQ80J95.
SMRiQ80J95. Positions 995-1167, 1187-1686.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ80J95.

Family & Domainsi

Sequence similaritiesi

Contains RdRp catalytic domain.SAAS annotation
Contains SF3 helicase domain.SAAS annotation

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR001665. Norovirus_pept_C37.
IPR027417. P-loop_NTPase.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
IPR013614. Viral_PP_Calicivir_N.
[Graphical view]
PfamiPF08405. Calici_PP_N. 1 hit.
PF05416. Peptidase_C37. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view]
PRINTSiPR00917. SRSVCYSPTASE.
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS51537. NV_3CL_PRO. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q80J95-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRMATPSSAP SVRNTEKRKN KKASSKASVS FGAPSPLSSE SEDEINYMTP
60 70 80 90 100
PEQEAQPGAL AALHAEGPLA GLPVTRSDAR VLIFNEWEER KKSDPWLRLD
110 120 130 140 150
MSDKAIFRRY PHLRPKEDRP DAPSHAEDAM DAKEPVIGSI LEQDDHKFYH
160 170 180 190 200
YSVYIGGGLV MGVNNPSAAV CQATIDVEKL HLWWRPVWEP RXPLDSAELR
210 220 230 240 250
KCVGMTVPYV ATTVNCYQVC CWIVGIKDTW LKRAKISRDL PFYSPVQDWN
260 270 280 290 300
VDPQEPFIPS KLRMVSDGIL VALSAVIGRP IKNLLASVKP LNILNIVLSC
310 320 330 340 350
DWTFSGIVNA LILLAELFDI FWTPPDVTNW MISIFGEWQA EGPFDLALDV
360 370 380 390 400
VPTLLGGIGM AFGLTSETIG RKLASTNSAL KAAQEMGKFA IEVFKQIMAW
410 420 430 440 450
IWPSEDPVPA LLSNMEQAII KNECQLENQL TAMLRDRNAG AEFLRSLDEE
460 470 480 490 500
EQEVRKIAAK CGNSATTGTT NALLARISMA RAAFEKARAE QTSRVRPVVI
510 520 530 540 550
MVSGRPGIGK TCFCQNLAKR IAASLGDETS VGIIPRADVD HWDAYKGARV
560 570 580 590 600
VLWDDFGMDN VVKDALRLQM LADTCPVTLN CDRIENKGKM FDSQVIIITT
610 620 630 640 650
NQQTPVPLDY VNLEAVCRRI DFLVYAESPV VDDARARAPG DVNAVKAAMR
660 670 680 690 700
PDYSHINFIL APQGGFDRQG NTPYGKGVTK IIGATALCAR AVALVHERHD
710 720 730 740 750
DFGLQNKVYD FDAGKVTAFK AMAADAGIPW YKMAAIGCKA MGCTCVEEAM
760 770 780 790 800
HLLKDYEVAP CQVIYNGATY NVSCIKGAPM VEKVKEPELP KTLVNCVRRI
810 820 830 840 850
KEARLRCYCR MAADVITSIL QAAGTAFSIY HQIEKRSRPS FYWDXGYTYR
860 870 880 890 900
DGPGSFDIFE DDDDGWYHSE GKKGKNKKGR GRPGVFRTRG LTDEEYDEFK
910 920 930 940 950
KRRESRGGKY SIDDYLADRE REEELLERDE EEAIFGDGFG LKATRRSRKA
960 970 980 990 1000
ERAKLGLVSG GDIRARKPID WNVVGPSWAD DDRQVDYGEK INFEAPVSIW
1010 1020 1030 1040 1050
SRVVQFGTGW GFWVSGHVFI TAKHVAPPKG TEIFGRKPGD FTVTSSGDFL
1060 1070 1080 1090 1100
KYYFTSAVRP DIPAMVLENG CQEGVVASVL VKRASGEMLA LAVRMGSQAA
1110 1120 1130 1140 1150
IKIGSAVVHG QTGMLLTGSN AKAQDLGTIP GDCGCPYVYK KGNTWVVIGV
1160 1170 1180 1190 1200
HVAATRSGNT VIAATHGEPT LEALEFQGPP MLPRPSGTYA GLPIADYGDA
1210 1220 1230 1240 1250
PPLSTKTMFW RTSPEKLPPG AWEPAYLGSK DERVDGPSLQ QVMRDQLKPY
1260 1270 1280 1290 1300
SEPRGLLPPQ EILDAVCDAI ENRLENTLEP QKPWTFKKAC ESLDKNTSSG
1310 1320 1330 1340 1350
YPYHKQKSKD WTGSAFIGDL GDQATHANNM YEMGKSMRPI YTAALKDELV
1360 1370 1380 1390 1400
KPDKIYGKIK KRLLWGSDLG TMIRAARAFG PFCDALKETC IFNPIRVGMS
1410 1420 1430 1440 1450
MNEDGPFIFA RHANFRYHMD ADYTRWDSTQ QRAILKRAGD IMVRLSPEPD
1460 1470 1480 1490 1500
LARVVMDDLL APSLLDVGDY KIVVEEGLPS GCPCTTQLNS LAHWILTLCA
1510 1520 1530 1540 1550
MVEVTRVDPD IVMQESEFSF YGDDEVVSTN LELDMVKYTM ALRRYGLLPT
1560 1570 1580 1590 1600
RADKEEGPLE RRQTLQGISF LRRAIVGDQF GWYGRLDRAS IDRQLLWTKG
1610 1620 1630 1640 1650
PNHQNPFETL PGHAQRPSQL MALLGEAAMH GEKYYRTVAS RVSKEAAQSG
1660 1670 1680
IEMVVPRHRS VLRWVRFGTM DAETPQERSA VFVNEDE
Length:1,687
Mass (Da):187,473
Last modified:December 6, 2005 - v2
Checksum:iEA493276296FDB23
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY228235 Genomic RNA. Translation: AAO63098.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY228235 Genomic RNA. Translation: AAO63098.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2M4GNMR-A881-955[»]
2MCDNMR-A28-114[»]
2MCHNMR-A58-114[»]
3NAHX-ray2.75A/B/C1181-1686[»]
3NAIX-ray2.56A/B/C1181-1686[»]
3QIDX-ray2.50A/B/C1181-1686[»]
3SFGX-ray2.21A/B/C1181-1686[»]
3SFUX-ray2.50A/B/C1181-1686[»]
3UPFX-ray2.60A/B/C1174-1687[»]
3UQSX-ray2.00A/B/C1181-1687[»]
3UR0X-ray2.45A/B/C1181-1687[»]
4ASHX-ray1.58A/B995-1177[»]
4NRUX-ray2.30A/B/C/D/E/F1181-1687[»]
4O4RX-ray2.40A/B/C1181-1687[»]
ProteinModelPortaliQ80J95.
SMRiQ80J95. Positions 995-1167, 1187-1686.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC37.003.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiQ80J95.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR001665. Norovirus_pept_C37.
IPR027417. P-loop_NTPase.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
IPR013614. Viral_PP_Calicivir_N.
[Graphical view]
PfamiPF08405. Calici_PP_N. 1 hit.
PF05416. Peptidase_C37. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view]
PRINTSiPR00917. SRSVCYSPTASE.
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS51537. NV_3CL_PRO. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "STAT1-dependent innate immunity to a Norwalk-like virus."
    Karst S.M., Wobus C.E., Lay M., Davidson J., Virgin H.W.
    Science 299:1575-1578(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Mu/NoV/GV/MNV1/2002/USAImported.
  2. "Development of a microsphere-based serologic multiplexed fluorescent immunoassay and a reverse transcriptase PCR assay to detect murine norovirus 1 infection in mice."
    Hsu C.C., Wobus C.E., Steffen E.K., Riley L.K., Livingston R.S.
    Clin. Diagn. Lab. Immunol. 12:1145-1151(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Mu/NoV/GV/MNV1/2002/USAImported.
  3. Karst S.M., Wobus C.E., Lay M., Davidson J., Virgin H.W.IV.
    Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Mu/NoV/GV/MNV1/2002/USAImported.
  4. "Crystal structures and functional analysis of murine norovirus RNA-dependent RNA polymerase."
    Kim K.H., Lee J.H., Alam I., Park Y., Kang S.
    Submitted (JUN-2010) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.75 ANGSTROMS) OF 1181-1686.
  5. "Crystal structures of murine norovirus-1 RNA-dependent RNA polymerase."
    Lee J.H., Alam I., Han K.R., Cho S., Shin S., Kang S., Yang J.M., Kim K.H.
    J. Gen. Virol. 92:1607-1616(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 1181-1686.
  6. Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 1181-1687, ACTIVE SITE.
  7. "Structure of a murine norovirus NS6 protease-product complex revealed by adventitious crystallisation."
    Leen E.N., Baeza G., Curry S.
    PLoS ONE 7:e38723-e38723(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.58 ANGSTROMS) OF 995-1177.
  8. "Crystal structures of murine norovirus-1 RNA-dependent RNA polymerase in complex with 2-thiouridine or ribavirin."
    Alam I., Lee J.H., Cho K.J., Han K.R., Yang J.M., Chung M.S., Kim K.H.
    Virology 426:143-151(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.21 ANGSTROMS) OF 1181-1686 IN COMPLEX WITH MAGNESIUM, ACTIVE SITE.
  9. "Structures of the compact helical core domains of feline calicivirus and murine norovirus VPg proteins."
    Leen E.N., Kwok K.Y., Birtley J.R., Simpson P.J., Subba-Reddy C.V., Chaudhry Y., Sosnovtsev S.V., Green K.Y., Prater S.N., Tong M., Young J.C., Chung L.M., Marchant J., Roberts L.O., Kao C.C., Matthews S., Goodfellow I.G., Curry S.
    J. Virol. 87:5318-5330(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 881-955.
  10. "PPNDS inhibits murine Norovirus RNA-dependent RNA-polymerase mimicking two RNA stacking bases."
    Croci R., Tarantino D., Milani M., Pezzullo M., Rohayem J., Bolognesi M., Mastrangelo E.
    FEBS Lett. 588:1720-1725(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 1181-1687, ACTIVE SITE.
  11. "Structural bases of norovirus RNA dependent RNA polymerase inhibition by novel suramin-related compounds."
    Croci R., Pezzullo M., Tarantino D., Milani M., Tsay S.C., Sureshbabu R., Tsai Y.J., Mastrangelo E., Rohayem J., Bolognesi M., Hwu J.R.
    PLoS ONE 9:e91765-e91765(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 1181-1687 IN COMPLEX WITH MAGNESIUM, ACTIVE SITE.
  12. "Murine norovirus protein NS1/2 aspartate to glutamate mutation, sufficient for persistence, reorients side chain of surface exposed tryptophan within a novel structured domain."
    Borin B.N., Tang W., Nice T.J., McCune B.T., Virgin H.W., Krezel A.M.
    Proteins 82:1200-1209(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 28-114.

Entry informationi

Entry nameiQ80J95_9CALI
AccessioniPrimary (citable) accession number: Q80J95
Entry historyi
Integrated into UniProtKB/TrEMBL: June 1, 2003
Last sequence update: December 6, 2005
Last modified: March 4, 2015
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.