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Q80IG9 (VP7_ROTB9) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Outer capsid glycoprotein VP7
OrganismRotavirus A (isolate Cow/Germany/993/1983 G18-P[17]-Ix-Rx-Cx-Mx-Ax-Nx-Tx-Ex-Hx) (RV-A)
Taxonomic identifier45408 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostBos taurus (Bovine) [TaxID: 9913]

Protein attributes

Sequence length329 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Outer capsid protein involved in attachment and possibly entry into the host epithelial cell. It is subsequently lost, together with VP4, following virus entry into the host cell. The outer layer contains 780 copies of VP7, grouped as 260 trimers. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. In integrin-dependent strains, VP7 seems to essentially target the integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3 at a postbinding stage, once the initial attachment by VP4 has been achieved By similarity.

Subunit structure

Homotrimer; in the presence of calcium By similarity. Acquisition of the capsid outer layer requires a high calcium concentration inside the endoplasmic reticulum. Following cell entry, the low calcium concentration in the cytoplasm is probably responsible for the solubilization of the outer layer. Interacts with host integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3 By similarity.

Subcellular location

Virion By similarity. Host endoplasmic reticulum lumen Potential. Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles By similarity.

Post-translational modification

The N-terminus is blocked possibly by pyroglutamic acid By similarity.

N-glycosylated By similarity.

Intramolecular disulfide bonds By similarity.

Miscellaneous

In group A rotaviruses, VP7 defines the G serotype.

Sequence similarities

Belongs to the rotavirus VP7 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 5353 Potential
Chain54 – 329276Outer capsid glycoprotein VP7 Potential
PRO_0000369095

Amino acid modifications

Glycosylation721N-linked (GlcNAc...); by host Potential
Glycosylation2411N-linked (GlcNAc...); by host Potential
Glycosylation3211N-linked (GlcNAc...); by host Potential

Sequences

Sequence LengthMass (Da)Tools
Q80IG9 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 2B203086D872C467

FASTA32937,178
        10         20         30         40         50         60 
MYSTECTILL IEIIFYFFAA VVVYDAIHKM ANSPIFCIAV LAVVFAVSPK CFAQNYGINV 

        70         80         90        100        110        120 
PITGSLDVAV PNKTDDQIGL TSSLCIYYPN EAEIEINDNE WKNTVAQLLL TKGWPTTSVY 

       130        140        150        160        170        180 
LNGYADLQSF SNNPQLNCDY NIVLVKYDQN AGLDMSELAE LLLYEWLCNE MDVNLYYYQQ 

       190        200        210        220        230        240 
TSEANKWIAM GSDCTIKVCP LNTQTLGIGC QTTDVATFEQ LTATEKLAII DVVDGVNHKV 

       250        260        270        280        290        300 
NYTIATCTLK NCIRLNQREN VAIIQVGGPE IIDVSEDPMI VPKMIRATRI NWKKWWQVFY 

       310        320 
TVVDYINTII QAMSKRSRSL NTSTYFLRI 

« Hide

References

[1]"Bovine rotavirus 993/83 shows a third subtype of avian VP7 protein."
Rohwedder A., Hotop H., Minamoto N., Ito H., Nakagomi O., Brussow H.
Virus Genes 14:147-151(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X98869 Genomic RNA. Translation: CAD83158.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR001963. VP7.
[Graphical view]
PfamPF00434. VP7. 1 hit.
[Graphical view]
ProDomPD000191. VP7. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Entry information

Entry nameVP7_ROTB9
AccessionPrimary (citable) accession number: Q80IG9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 1, 2003
Last modified: February 19, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families