Neuraminidase - Influenza A virus (A/Memphis/31/98(H3N2))

Preferred order of sections

Names and origin

Protein names	Submitted name: Neuraminidase EMBL AAP23239.1 EC=3.2.1.18 EMBL AAP23239.1
Organism	Influenza A virus (A/Memphis/31/98(H3N2)) EMBL AAP23239.1
Taxonomic identifier	228928 [NCBI]
Taxonomic lineage	Viruses › ssRNA viruses › ssRNA negative-strand viruses › Orthomyxoviridae › Influenzavirus A › Influenza A virus › H3N2 subtype

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates. SAAS SAAS011040
Subunit structure	Homotetramer By similarity. SAAS SAAS011040

Ontologies

Keywords
Cellular component	Host cell membrane SAAS SAAS011040 Host membrane Membrane Virion SAAS SAAS011040
Domain	Transmembrane Transmembrane helix SAAS SAAS011040
Molecular function	Glycosidase SAAS SAAS011040 EMBL AAP23239.1 Hydrolase
PTM	Disulfide bond SAAS SAAS011040
Technical term	3D-structure PDB 2AEQ
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	host cell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW virion membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	exo-alpha-(2->3)-sialidase activity Inferred from electronic annotation. Source: EC exo-alpha-(2->6)-sialidase activity Inferred from electronic annotation. Source: EC exo-alpha-(2->8)-sialidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Regions

Region

393 – 394

2

N-acetyl-D-glucosamine binding PDB 2AEQ

Sites

Binding site

86

1

N-acetyl-D-glucosamine PDB 2AEQ

Binding site

200

1

N-acetyl-D-glucosamine PDB 2AEQ

Binding site

234

1

N-acetyl-D-glucosamine PDB 2AEQ

Binding site

284

1

N-acetyl-D-glucosamine PDB 2AEQ

Binding site

391

1

Mannose PDB 2AEQ

Binding site

437

1

N-acetyl-D-glucosamine PDB 2AEQ

Binding site

454

1

N-acetyl-D-glucosamine; via carbonyl oxygen PDB 2AEQ

Sequences

Sequence

Length

Mass (Da)

Tools

Q80DL0 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 46B453F12B0D2754
Show »

FASTA

469

52,110

        10         20         30         40         50         60 
MNPNQKIITI GSVSLTIATI CFLMQIAILV TTVTLHFKQY ECSSPPNNQV MLCEPTIIER 

        70         80         90        100        110        120 
NITEIVYLTN TTIEKEICPK LAEYRNWSKP QCKITGFAPF SKDNSIRLSA GGDIWVTREP 

       130        140        150        160        170        180 
YVSCDPDKCY QFALGQGTTL NNRHSNDTVH DRTPYRTLLM NELGVPFHLG TKQVCIAWSS 

       190        200        210        220        230        240 
SSCHDGKAWL HVCVTGHDEN ATASFIYDGR LVDSIGSWSK KILRTQESEC VCINGTCTVV 

       250        260        270        280        290        300 
MTDGSASGRA DTKILFIEEG KIVHISPLSG SAQHVEECSC YPRYPGVRCV CRDNWKGSNR 

       310        320        330        340        350        360 
PIVDINVKDY SIVSSYVCSG LVGDTPRKND SSSSSHCLNP NNEEGGHGVK GWAFDDGNDV 

       370        380        390        400        410        420 
WMGRTISEKF RSGYETFKVI EGWSKPNSKL QINRQVIVDR GNRSGYSGIF SVEGKSCINR 

       430        440        450        460 
CFYVELIRGR KQETEVWWTS NSIVVFCGTS GTYGTGSWPD GADINLMPI

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References

[1]	"Antibody epitopes on the neuraminidase of a recent H3N2 influenza virus (A/Memphis/31/98)." Gulati U., Hwang C.C., Venkatramani L., Gulati S., Stray S.J., Lee J.T., Laver W.G., Bochkarev A., Zlotnick A., Air G.M. J. Virol. 76:12274-12280(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	Gulati U., Air G.M. Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
[3]	"An epidemiologically significant epitope of a 1998 human influenza virus neuraminidase forms a highly hydrated interface in the NA-antibody complex." Venkatramani L., Bochkareva E., Lee J.T., Gulati U., Graeme Laver W., Bochkarev A., Air G.M. J. Mol. Biol. 356:651-663(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS) OF 75-469 IN COMPLEX WITH MANNOSE AND N-ACETYL-D-GLUCOSAMINE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY271795 Genomic RNA. Translation: AAP23239.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2AEQ	X-ray	3.00	A	75-469	[»]

ProteinModelPortal

Q80DL0.

SMR

Q80DL0. Positions 82-469.

ModBase

Search...

Protein family/group databases

CAZy

GH34. Glycoside Hydrolase Family 34.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

2.120.10.10. 1 hit.

InterPro

IPR001860. Glyco_hydro_34.
IPR011040. Sialidases.
[Graphical view]

Pfam

PF00064. Neur. 1 hit.
[Graphical view]

SUPFAM

SSF50939. Sialidase. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q80DL0.

Entry information

Entry name

Q80DL0_9INFA

Accession

Primary (citable) accession number: Q80DL0

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 2003
Last sequence update:	June 1, 2003
Last modified:	April 3, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)