Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Neuraminidase

Gene

NA

Organism
Influenza A virus (A/Memphis/31/98(H3N2))
Status
Unreviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.UniRule annotationSAAS annotation

Cofactori

Ca2+UniRule annotationSAAS annotation

Enzyme regulationi

Inhibited by the neuraminidase inhibitors zanamivir (Relenza) and oseltamivir (Tamiflu). These drugs interfere with the release of progeny virus from infected cells and are effective against all influenza strains. Resistance to neuraminidase inhibitors is quite rare.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei86N-acetyl-D-glucosamineCombined sources1
Binding sitei118SubstrateUniRule annotation1
Active sitei151Proton donor/acceptorUniRule annotation1
Binding sitei152SubstrateUniRule annotation1
Binding sitei200N-acetyl-D-glucosamineCombined sources1
Binding sitei234N-acetyl-D-glucosamineCombined sources1
Binding sitei284N-acetyl-D-glucosamineCombined sources1
Binding sitei292SubstrateUniRule annotation1
Metal bindingi293Calcium; via carbonyl oxygenUniRule annotation1
Metal bindingi297Calcium; via carbonyl oxygenUniRule annotation1
Metal bindingi324CalciumUniRule annotation1
Binding sitei371SubstrateUniRule annotation1
Active sitei406NucleophileUniRule annotation1
Binding sitei437N-acetyl-D-glucosamineCombined sources1
Binding sitei454N-acetyl-D-glucosamine; via carbonyl oxygenCombined sources1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidaseUniRule annotationSAAS annotationImported, Hydrolase
LigandCalciumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation

Protein family/group databases

CAZyiGH34. Glycoside Hydrolase Family 34.

Names & Taxonomyi

Protein namesi
Recommended name:
NeuraminidaseUniRule annotationSAAS annotation (EC:3.2.1.18UniRule annotationSAAS annotation)
Gene namesi
Name:NAUniRule annotation
OrganismiInfluenza A virus (A/Memphis/31/98(H3N2))Imported
Taxonomic identifieri228928 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A

Subcellular locationi

  • Host apical cell membrane SAAS annotation; Single-pass type II membrane protein SAAS annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei12 – 37HelicalUniRule annotationAdd BLAST26

GO - Cellular componenti

Keywords - Cellular componenti

Host cell membraneUniRule annotationSAAS annotation, Host membrane, Membrane, VirionUniRule annotationSAAS annotation

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi92 ↔ 417UniRule annotationCombined sources
Disulfide bondi124 ↔ 129UniRule annotationCombined sources
Disulfide bondi175 ↔ 193Combined sources
Disulfide bondi183 ↔ 230UniRule annotationCombined sources
Disulfide bondi232 ↔ 237UniRule annotationCombined sources
Disulfide bondi278 ↔ 291UniRule annotationCombined sources
Disulfide bondi280 ↔ 289UniRule annotationCombined sources
Disulfide bondi318 ↔ 337Combined sources
Disulfide bondi421 ↔ 447Combined sources

Post-translational modificationi

N-glycosylated.UniRule annotation

Keywords - PTMi

Disulfide bondUniRule annotationSAAS annotation, GlycoproteinUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotationSAAS annotation

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AEQX-ray3.00A75-469[»]
ProteinModelPortaliQ80DL0.
SMRiQ80DL0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ80DL0.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni11 – 33Involved in apical transport and lipid raft associationUniRule annotationAdd BLAST23
Regioni91 – 469Head of neuraminidaseUniRule annotationAdd BLAST379
Regioni276 – 277Substrate bindingUniRule annotation2
Regioni393 – 394N-acetyl-D-glucosamine bindingCombined sources2

Domaini

Intact N-terminus is essential for virion morphogenesis. Possess two apical sorting signals, one in the ectodomain, which is likely to be a glycan, and the other in the transmembrane domain. The transmembrane domain also plays a role in lipid raft association.UniRule annotation

Sequence similaritiesi

Belongs to the glycosyl hydrolase 34 family.UniRule annotationSAAS annotation

Keywords - Domaini

Signal-anchorUniRule annotation, Transmembrane, Transmembrane helixUniRule annotationSAAS annotation

Family and domain databases

CDDicd15483. Influenza_NA. 1 hit.
HAMAPiMF_04071. INFV_NRAM. 1 hit.
InterProiView protein in InterPro
IPR001860. Glyco_hydro_34.
IPR033654. Sialidase_Influenza_A/B.
IPR036278. Sialidase_sf.
PfamiView protein in Pfam
PF00064. Neur. 1 hit.
SUPFAMiSSF50939. SSF50939. 1 hit.

Sequencei

Sequence statusi: Complete.

Q80DL0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPNQKIITI GSVSLTIATI CFLMQIAILV TTVTLHFKQY ECSSPPNNQV
60 70 80 90 100
MLCEPTIIER NITEIVYLTN TTIEKEICPK LAEYRNWSKP QCKITGFAPF
110 120 130 140 150
SKDNSIRLSA GGDIWVTREP YVSCDPDKCY QFALGQGTTL NNRHSNDTVH
160 170 180 190 200
DRTPYRTLLM NELGVPFHLG TKQVCIAWSS SSCHDGKAWL HVCVTGHDEN
210 220 230 240 250
ATASFIYDGR LVDSIGSWSK KILRTQESEC VCINGTCTVV MTDGSASGRA
260 270 280 290 300
DTKILFIEEG KIVHISPLSG SAQHVEECSC YPRYPGVRCV CRDNWKGSNR
310 320 330 340 350
PIVDINVKDY SIVSSYVCSG LVGDTPRKND SSSSSHCLNP NNEEGGHGVK
360 370 380 390 400
GWAFDDGNDV WMGRTISEKF RSGYETFKVI EGWSKPNSKL QINRQVIVDR
410 420 430 440 450
GNRSGYSGIF SVEGKSCINR CFYVELIRGR KQETEVWWTS NSIVVFCGTS
460
GTYGTGSWPD GADINLMPI
Length:469
Mass (Da):52,110
Last modified:June 1, 2003 - v1
Checksum:i46B453F12B0D2754
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY271795 Genomic RNA. Translation: AAP23239.1.

Similar proteinsi

Entry informationi

Entry nameiQ80DL0_9INFA
AccessioniPrimary (citable) accession number: Q80DL0
Entry historyiIntegrated into UniProtKB/TrEMBL: June 1, 2003
Last sequence update: June 1, 2003
Last modified: March 28, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

3D-structureCombined sources