Reviewed, UniProtKB/Swiss-Prot Q805R1 (GCNT3_BHV4L)

Names and origin

Protein names

Recommended name:
    Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase
    EC=2.4.1.102
    EC=2.4.1.150
Alternative name(s):
    C2GnT-mucin type
      Short name=C2GnT-M

Gene names

Name:

Bo17

Organism

Bovine herpesvirus 4 (strain LVR140) (BoHV-4) (Movar virus)

Taxonomic identifier

436506 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Gammaherpesvirinae › Rhadinovirus

Virus host

Bos taurus (Bovine) [TaxID: 9913]

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Non-essential glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans. Contributes to the post-translational modifications of structural proteins.
Catalytic activity	UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R. UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Host Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Developmental stage	Expressed during BHV-4 repkication (at protein level).
Miscellaneous	Was acquired from an ancestor of the African buffalo around 1.5 million years ago.
Sequence similarities	Belongs to the glycosyltransferase 14 family.

Ontologies

Keywords
Cellular component	Host Golgi apparatus Host membrane Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 440	440	Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase		PRO_0000288553
Regions
	Topological domain	1 – 9	9	Cytoplasmic Potential
	Transmembrane	10 – 30	21	Signal-anchor for type II membrane Potential
	Topological domain	31 – 440	410	Lumenal Potential
Amino acid modifications
	Glycosylation	72	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	108	1	N-linked (GlcNAc...); by host Potential
	Disulfide bond	73 ↔ 230		By similarity
	Disulfide bond	164 ↔ 384		By similarity
	Disulfide bond	185 ↔ 212		By similarity
	Disulfide bond	393 ↔ 425		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q805R1-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 1B79C51ADC85633D	FASTA	440	50,720
	10 20 30 40 50 60 MKMAGWKKKL CPGHHLWALG CYMLLAVVSL RLSLRFKCDV DSLDLESRDF QSQHCRDMLY 70 80 90 100 110 120 NSLKLPAKRS INCSGITRGD QEAVVQALLD NLEVKKKRPP LTDTYYLNIT RDCERFKAQR 130 140 150 160 170 180 KFIQFPLSKE ELDFPIAYSM VVHEKIENFE RLLRAVYAPQ NIYCVHVDVK SPETFKEAVK 190 200 210 220 230 240 AIISCFPNVF MASKLVPVVY ASWSRVQADL NCMEDLLQSS VSWKYLLNTC GTDFPIKTNA 250 260 270 280 290 300 EMVLALKMLK GKNSMESEVP SESKKNRWKY RYEVTDTLYP TSKMKDPPPD NLPMFTGNAY 310 320 330 340 350 360 FVASRAFVQH VLDNPKSQIL VEWVKDTYSP DEHLWATLQR APWMPGSVPS HPKYHISDMT 370 380 390 400 410 420 AIARLVKWQY HEGDVSMGAP YAPCSGIHRR AICIYGAGDL YWILQNHHLL ANKFDPRVDD 430 440 NVLQCLEEYL RHKAIYGTEL

References

[1]

"The core 2 beta-1,6-N-acetylglucosaminyltransferase-mucin encoded by bovine herpesvirus 4 was acquired from an ancestor of the African buffalo." Markine-Goriaynoff N., Georgin J.-P., Goltz M., Zimmermann W., Broll H., Wamwayi H.M., Pastoret P.-P., Sharp P.M., Vanderplasschen A. J. Virol. 77:1784-1792(2003) [PubMed: 12525612] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF465330 Genomic DNA. Translation: AAO22157.1. AF465331 Genomic DNA. Translation: AAO22158.1.
3D structure databases
SMR	Q805R1. Positions 70-435.
ModBase	Search...
Protein family/group databases
CAZy	GT14. Glycosyltransferase Family 14.
Family and domain databases
InterPro	IPR021141. Core-2/I-Branching_enzyme. IPR003406. Glyco_trans_14. [Graphical view]
PANTHER	PTHR19297. Glyco_trans_14. 1 hit.
Pfam	PF02485. Branch. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCNT3_BHV4L

Accession

Primary (citable) accession number: Q805R1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)