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Q805R1

- GCNT3_BHV4L

UniProt

Q805R1 - GCNT3_BHV4L

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Protein

Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase

Gene

Bo17

Organism
Bovine herpesvirus 4 (strain LVR140) (BoHV-4) (Movar virus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Non-essential glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans. Contributes to the post-translational modifications of structural proteins.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R.
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.

Pathwayi

GO - Molecular functioni

  1. beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC
  2. N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT14. Glycosyltransferase Family 14.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase (EC:2.4.1.102, EC:2.4.1.150)
Alternative name(s):
C2GnT-mucin type
Short name:
C2GnT-M
Gene namesi
Name:Bo17
OrganismiBovine herpesvirus 4 (strain LVR140) (BoHV-4) (Movar virus)
Taxonomic identifieri436506 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeRhadinovirus
Virus hostiBos taurus (Bovine) [TaxID: 9913]

Subcellular locationi

GO - Cellular componenti

  1. host cell Golgi apparatus Source: UniProtKB-KW
  2. host cell membrane Source: UniProtKB-KW
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host Golgi apparatus, Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 440440Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferasePRO_0000288553Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi72 – 721N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi73 ↔ 230By similarity
Glycosylationi108 – 1081N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi164 ↔ 384By similarity
Disulfide bondi185 ↔ 212By similarity
Disulfide bondi393 ↔ 425By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Developmental stagei

Expressed during BHV-4 repkication (at protein level).

Structurei

3D structure databases

ProteinModelPortaliQ805R1.
SMRiQ805R1. Positions 70-435.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 99CytoplasmicSequence Analysis
Topological domaini31 – 440410LumenalSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei10 – 3021Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 14 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR003406. Glyco_trans_14.
[Graphical view]
PfamiPF02485. Branch. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q805R1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKMAGWKKKL CPGHHLWALG CYMLLAVVSL RLSLRFKCDV DSLDLESRDF
60 70 80 90 100
QSQHCRDMLY NSLKLPAKRS INCSGITRGD QEAVVQALLD NLEVKKKRPP
110 120 130 140 150
LTDTYYLNIT RDCERFKAQR KFIQFPLSKE ELDFPIAYSM VVHEKIENFE
160 170 180 190 200
RLLRAVYAPQ NIYCVHVDVK SPETFKEAVK AIISCFPNVF MASKLVPVVY
210 220 230 240 250
ASWSRVQADL NCMEDLLQSS VSWKYLLNTC GTDFPIKTNA EMVLALKMLK
260 270 280 290 300
GKNSMESEVP SESKKNRWKY RYEVTDTLYP TSKMKDPPPD NLPMFTGNAY
310 320 330 340 350
FVASRAFVQH VLDNPKSQIL VEWVKDTYSP DEHLWATLQR APWMPGSVPS
360 370 380 390 400
HPKYHISDMT AIARLVKWQY HEGDVSMGAP YAPCSGIHRR AICIYGAGDL
410 420 430 440
YWILQNHHLL ANKFDPRVDD NVLQCLEEYL RHKAIYGTEL
Length:440
Mass (Da):50,720
Last modified:June 1, 2003 - v1
Checksum:i1B79C51ADC85633D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF465330 Genomic DNA. Translation: AAO22157.1.
AF465331 Genomic DNA. Translation: AAO22158.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF465330 Genomic DNA. Translation: AAO22157.1 .
AF465331 Genomic DNA. Translation: AAO22158.1 .

3D structure databases

ProteinModelPortali Q805R1.
SMRi Q805R1. Positions 70-435.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GT14. Glycosyltransferase Family 14.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00378 .

Family and domain databases

InterProi IPR003406. Glyco_trans_14.
[Graphical view ]
Pfami PF02485. Branch. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The core 2 beta-1,6-N-acetylglucosaminyltransferase-mucin encoded by bovine herpesvirus 4 was acquired from an ancestor of the African buffalo."
    Markine-Goriaynoff N., Georgin J.-P., Goltz M., Zimmermann W., Broll H., Wamwayi H.M., Pastoret P.-P., Sharp P.M., Vanderplasschen A.
    J. Virol. 77:1784-1792(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiGCNT3_BHV4L
AccessioniPrimary (citable) accession number: Q805R1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: June 1, 2003
Last modified: October 29, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Was acquired from an ancestor of the African buffalo around 1.5 million years ago.

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3