Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Disintegrin acostatin-alpha

Gene
N/A
Organism
Agkistrodon contortrix contortrix (Southern copperhead)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits fibrinogen interaction with platelets. Acts by binding to alpha-IIb/beta-3 (ITGA2B/ITGB3) on the platelet surface and inhibits ADP-induced platelet aggregation in human platelet-rich plasma.1 Publication

GO - Biological processi

  • cell adhesion Source: UniProtKB
  • negative regulation of blood coagulation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Cell adhesion impairing toxin, Hemostasis impairing toxin, Platelet aggregation inhibiting toxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Disintegrin acostatin-alpha
Cleaved into the following chain:
OrganismiAgkistrodon contortrix contortrix (Southern copperhead)Imported
Taxonomic identifieri8713 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeAgkistrodon

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Propeptidei21 – 46261 PublicationPRO_0000007236Add
BLAST
Chaini47 – 10963Disintegrin acostatin-alphaPRO_0000007237Add
BLAST
Chaini48 – 10962Disintegrin acostatin-alpha, processed formPRO_0000413008Add
BLAST
Propeptidei110 – 1112PRO_0000007238

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei48 – 481Pyrrolidone carboxylic acid; in Disintegrin acostatin-alpha, processed form2 Publications
Disulfide bondi53 ↔ 76PROSITE-ProRule annotation1 Publication
Disulfide bondi54 – 54Interchain (with C-431 in subunit beta)PROSITE-ProRule annotation1 Publication
Disulfide bondi59 – 59Interchain (with C-426 in subunit beta)PROSITE-ProRule annotation1 Publication
Disulfide bondi67 ↔ 73PROSITE-ProRule annotation1 Publication
Disulfide bondi72 ↔ 97PROSITE-ProRule annotation1 Publication
Disulfide bondi85 ↔ 104PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Heterodimer with subunit beta; disulfide-linked.2 Publications

Structurei

Secondary structure

1
111
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni56 – 594Combined sources
Beta strandi68 – 703Combined sources
Beta strandi84 – 863Combined sources
Beta strandi89 – 924Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3C05X-ray1.70A/C49-109[»]
ProteinModelPortaliQ805F7.
SMRiQ805F7. Positions 51-109.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ805F7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini47 – 11165DisintegrinPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi89 – 913Cell attachment site

Sequence similaritiesi

Contains 1 disintegrin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG051359.

Family and domain databases

Gene3Di4.10.70.10. 1 hit.
InterProiIPR018358. Disintegrin_CS.
IPR001762. Disintegrin_dom.
[Graphical view]
PfamiPF00200. Disintegrin. 1 hit.
[Graphical view]
PRINTSiPR00289. DISINTEGRIN.
SMARTiSM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q805F7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIQVLLVTLC LAVFPYQGSS IILESGNVND YEVVYPRKVT ALPKGAIQPK
60 70 80 90 100
NPCCDAATCK LTPGSQCAEG LCCDQCKFIK AGKICRRARG DNPDYRCTGQ
110
SGDCPRKHFY A
Length:111
Mass (Da):12,093
Last modified:June 1, 2003 - v1
Checksum:i5D120A539592659C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB078903 mRNA. Translation: BAC55944.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB078903 mRNA. Translation: BAC55944.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3C05X-ray1.70A/C49-109[»]
ProteinModelPortaliQ805F7.
SMRiQ805F7. Positions 51-109.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG051359.

Miscellaneous databases

EvolutionaryTraceiQ805F7.

Family and domain databases

Gene3Di4.10.70.10. 1 hit.
InterProiIPR018358. Disintegrin_CS.
IPR001762. Disintegrin_dom.
[Graphical view]
PfamiPF00200. Disintegrin. 1 hit.
[Graphical view]
PRINTSiPR00289. DISINTEGRIN.
SMARTiSM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A new gene structure of the disintegrin family: a subunit of dimeric disintegrin has a short coding region."
    Okuda D., Koike H., Morita T.
    Biochemistry 41:14248-14254(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 47-109, PYROGLUTAMATE FORMATION AT GLN-48 IN PROCESSED FORM, FUNCTION, SUBUNIT.
    Tissue: Venom1 Publication and Venom gland.
  2. "Crystallization and preliminary crystallographic studies of dimeric disintegrins from the venom of two Agkistrodon snakes."
    Fujii Y., Okuda D., Fujimoto Z., Morita T., Mizuno H.
    Acta Crystallogr. D 58:145-147(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: CRYSTALLIZATION.
    Tissue: Venom.
  3. "Structure of acostatin, a dimeric disintegrin from Southern copperhead (Agkistrodon contortrix contortrix), at 1.7 A resolution."
    Moiseeva N., Bau R., Swenson S.D., Markland F.S. Jr., Choe J.Y., Liu Z.J., Allaire M.
    Acta Crystallogr. D 64:466-470(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 48-109, DISULFIDE BONDS, PYROGLUTAMATE FORMATION AT GLN-48.

Entry informationi

Entry nameiDIDA_AGKCO
AccessioniPrimary (citable) accession number: Q805F7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 1, 2003
Last modified: October 14, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.