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Protein

Zinc metalloproteinase/disintegrin

Gene
N/A
Organism
Agkistrodon contortrix contortrix (Southern copperhead)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Snake venom metalloproteinase: impairs hemostasis in the envenomed animal (By similarity). This product has not been identified in the venom.By similarity
Disintegrin acostatin-beta: inhibits platelet aggregation in human platelet-rich plasma induced by ADP. Acts by inhibiting fibrinogen interaction with platelet receptors GPIIb/GPIIIa (ITGA2B/ITGB3).

Miscellaneous

The disintegrin belongs to the dimeric disintegrin subfamily.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi201CalciumBy similarity1
Metal bindingi285CalciumBy similarity1
Metal bindingi334Zinc; catalyticBy similarity1
Active sitei335PROSITE-ProRule annotation1
Metal bindingi338Zinc; catalyticBy similarity1
Metal bindingi344Zinc; catalyticBy similarity1
Metal bindingi389Calcium; via carbonyl oxygenBy similarity1
Metal bindingi392CalciumBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionCell adhesion impairing toxin, Hemostasis impairing toxin, Hydrolase, Metalloprotease, Platelet aggregation inhibiting toxin, Protease, Toxin
LigandCalcium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc metalloproteinase/disintegrin
Cleaved into the following 2 chains:
OrganismiAgkistrodon contortrix contortrix (Southern copperhead)Imported
Taxonomic identifieri8713 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeAgkistrodon

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
PropeptideiPRO_000002896521 – 191By similarityAdd BLAST171
ChainiPRO_0000028966192 – 394Snake venom metalloproteinaseBy similarityAdd BLAST203
PropeptideiPRO_0000028967395 – 4181 PublicationAdd BLAST24
ChainiPRO_0000028968419 – 483Disintegrin acostatin-betaAdd BLAST65

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi309 ↔ 389By similarity
Disulfide bondi349 ↔ 373By similarity
Disulfide bondi351 ↔ 356By similarity
Disulfide bondi425 ↔ 4481 Publication
Disulfide bondi426Interchain (with C-59 in alpha subunit)PROSITE-ProRule annotation1 Publication
Disulfide bondi431Interchain (with C-54 in alpha subunit)PROSITE-ProRule annotation1 Publication
Disulfide bondi439 ↔ 4451 Publication
Disulfide bondi444 ↔ 4691 Publication
Disulfide bondi457 ↔ 4761 Publication

Keywords - PTMi

Disulfide bond, Zymogen

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Heterodimer with subunit alpha; disulfide-linked (disintegrin).2 Publications

Structurei

Secondary structure

1483
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni428 – 431Combined sources4
Beta strandi440 – 442Combined sources3
Beta strandi456 – 458Combined sources3
Beta strandi461 – 464Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3C05X-ray1.70B/D419-482[»]
ProteinModelPortaliQ805F6.
SMRiQ805F6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ805F6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini198 – 394Peptidase M12BPROSITE-ProRule annotationAdd BLAST197
Domaini402 – 483DisintegrinPROSITE-ProRule annotationAdd BLAST82

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi461 – 463Cell attachment site3

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG006978.

Family and domain databases

CDDicd04269. ZnMc_adamalysin_II_like. 1 hit.
Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiView protein in InterPro
IPR018358. Disintegrin_CS.
IPR001762. Disintegrin_dom.
IPR036436. Disintegrin_dom_sf.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
IPR034027. Reprolysin_adamalysin.
PfamiView protein in Pfam
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
PRINTSiPR00289. DISINTEGRIN.
SMARTiView protein in SMART
SM00050. DISIN. 1 hit.
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiView protein in PROSITE
PS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q805F6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIQVLLVTLC LAAFPYQGSS IILESGNVND YEVLYPQKVI ALPKGAVQPK
60 70 80 90 100
YEDTMQYEFK VNGEPVVLHL EKNKGLFSKD YSETHYSSDG RKITTNPPVE
110 120 130 140 150
DHCYYHGRIQ NDADSTASIS ACNGLKGHFK LQGETYLIEP LKLPDSEAHA
160 170 180 190 200
VYKYENVEKE DEAPKMCGVT QTNWKSDKPI KKASQLNLTP EQQGFPQRYI
210 220 230 240 250
ELVVVADHRM FTKYNSNLNT IRIWVHELVN TMNVFYRPLN IRVSLTDLEV
260 270 280 290 300
WSNRDLINVQ PAAADTLEAF GDWRETVLLN RISHDNAQLL TAIELDGETI
310 320 330 340 350
GLANRGTMCD PKLSTGIVQD HSAINLWVAV TMAHEMGHNL GISHDGNQCH
360 370 380 390 400
CDANSCIMSE ELREQLSFEF SDCSQNQYQT YLTDHNPQCM LNEPLRTDIV
410 420 430 440 450
STPVSGNELL ETGEESDFDA PANPCCDAAT CKLTTGSQCA DGLCCDQCKF
460 470 480
MKEGTVCRRA RGDDLDDYCN GISAGCPRNP FHA
Length:483
Mass (Da):54,026
Last modified:June 1, 2003 - v1
Checksum:i6D91926570971221
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB078904 mRNA. Translation: BAC55945.1.

Similar proteinsi

Entry informationi

Entry nameiVM2AB_AGKCO
AccessioniPrimary (citable) accession number: Q805F6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: June 1, 2003
Last modified: October 25, 2017
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families