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Protein

Zinc metalloproteinase/disintegrin

Gene
N/A
Organism
Agkistrodon piscivorus piscivorus (Eastern cottonmouth)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Snake venom metalloproteinase: impairs hemostasis in the envenomed animal.By similarity
Disintegrin piscivostatin-beta: inhibits platelet aggregation induced by ADP. Acts by inhibiting fibrinogen interaction with platelet receptors GPIIb/GPIIIa (ITGA2B/ITGB3). Also inhibits platelet aggregate dissociation in human platelet-rich plasma.1 Publication

Miscellaneous

The disintegrin belongs to the dimeric disintegrin subfamily.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi201CalciumBy similarity1
Metal bindingi285CalciumBy similarity1
Metal bindingi334Zinc; catalytic1
Active sitei335PROSITE-ProRule annotation1
Metal bindingi338Zinc; catalytic1
Metal bindingi344Zinc; catalytic1
Metal bindingi389Calcium; via carbonyl oxygenBy similarity1
Metal bindingi392CalciumBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionCell adhesion impairing toxin, Hemostasis impairing toxin, Hydrolase, Metalloprotease, Platelet aggregation inhibiting toxin, Protease, Toxin
LigandCalcium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc metalloproteinase/disintegrin
Cleaved into the following 2 chains:
OrganismiAgkistrodon piscivorus piscivorus (Eastern cottonmouth)Imported
Taxonomic identifieri8716 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeAgkistrodon

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
PropeptideiPRO_000002896921 – 191By similarityAdd BLAST171
ChainiPRO_0000028970192 – 394Snake venom metalloproteinaseAdd BLAST203
PropeptideiPRO_0000028971395 – 414By similarityAdd BLAST20
ChainiPRO_0000028972415 – 483Disintegrin piscivostatin-betaAdd BLAST69

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi309 ↔ 389By similarity
Disulfide bondi349 ↔ 373By similarity
Disulfide bondi351 ↔ 356By similarity
Disulfide bondi425 ↔ 448By similarity
Disulfide bondi426Interchain (with C-54 in alpha subunit)PROSITE-ProRule annotationBy similarity
Disulfide bondi431Interchain (with C-59 in alpha subunit)PROSITE-ProRule annotationBy similarity
Disulfide bondi439 ↔ 445By similarity
Disulfide bondi444 ↔ 469By similarity
Disulfide bondi457 ↔ 476By similarity

Keywords - PTMi

Disulfide bond, Zymogen

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Heterodimer with piscivostatin-alpha; disulfide-linked (disintegrin).1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ805F4.
SMRiQ805F4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini198 – 394Peptidase M12BPROSITE-ProRule annotationAdd BLAST197
Domaini402 – 483DisintegrinPROSITE-ProRule annotationAdd BLAST82

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi461 – 463Cell attachment site; atypical (KGD)3

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG006978.

Family and domain databases

CDDicd04269. ZnMc_adamalysin_II_like. 1 hit.
Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiView protein in InterPro
IPR001762. Disintegrin_dom.
IPR036436. Disintegrin_dom_sf.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
IPR034027. Reprolysin_adamalysin.
PfamiView protein in Pfam
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
PRINTSiPR00289. DISINTEGRIN.
SMARTiView protein in SMART
SM00050. DISIN. 1 hit.
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiView protein in PROSITE
PS50215. ADAM_MEPRO. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q805F4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIQVLLVTLC LAAFPYQGNS IILESGNVND YEVLYPQKVT ALPKGAVQPK
60 70 80 90 100
YEDTMQYEFK VNGEPVVLHL EKNKGLFSKD YSETHYSSDG RKITTNPPVE
110 120 130 140 150
DHCYYHGRIQ NDADSTASIS ACNGLKGHFK LQGETYLIEP LKLSDSEAHA
160 170 180 190 200
VYKYENVEKE DEAPKMCGVT QTNWKSDKPI KKASQLNLTP EQQRFPQRYI
210 220 230 240 250
ELVVVADHRM FTKYNGNLNT IRIWVHELVN TMNVFYRPLN IHVSLTDLEV
260 270 280 290 300
WSDQDLINVQ PAAADTLEAF GDWRETVLLN RISHDNAQLL TAIELDGETI
310 320 330 340 350
GLANRGTMCD PKLSTGIVQD HSAINLWVAV TMAHEMGHNL GISHDGNQCH
360 370 380 390 400
CDANSCIMSE ELRQQLSFEF SDCSQNQYQT FLTDHNPQCM LNEPLRTDIV
410 420 430 440 450
STPVSGNELW ETGEESDFDA PANPCCDAAT CKLTPGSQCA EGLCCDQCKF
460 470 480
MKEGTVCHRA KGDDLDDYCN GISAGCPRNP FHA
Length:483
Mass (Da):54,073
Last modified:June 1, 2003 - v1
Checksum:iF1DA9CE0F00DE3E7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB078906 mRNA. Translation: BAC55947.1.

Similar proteinsi

Entry informationi

Entry nameiVM2PB_AGKPI
AccessioniPrimary (citable) accession number: Q805F4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: June 1, 2003
Last modified: October 25, 2017
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families