Q802T2 (ALG6_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase
EC=2.4.1.267

Alternative name(s):
Asparagine-linked glycosylation protein 6 homolog
Dol-P-Glc:Man(9)GlcNAc(2)-PP-Dol alpha-1,3-glucosyltransferase
Dolichyl-P-Glc:Man9GlcNAc2-PP-dolichyl glucosyltransferase

Gene names

Name:	ALG6
ORF Names:	RCJMB04_6f21

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	507 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Adds the first glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Man9GlcNAc(2)-PP-Dol By similarity.
Catalytic activity	Dolichyl beta-D-glucosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the ALG6/ALG8 glucosyltransferase family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring hexosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 507	507	Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase		PRO_0000284134
Regions
	Transmembrane	4 – 24	21	Helical; Potential
	Transmembrane	115 – 135	21	Helical; Potential
	Transmembrane	144 – 164	21	Helical; Potential
	Transmembrane	173 – 193	21	Helical; Potential
	Transmembrane	227 – 247	21	Helical; Potential
	Transmembrane	298 – 318	21	Helical; Potential
	Transmembrane	324 – 344	21	Helical; Potential
	Transmembrane	362 – 382	21	Helical; Potential
	Transmembrane	388 – 408	21	Helical; Potential
	Transmembrane	438 – 458	21	Helical; Potential
	Transmembrane	473 – 493	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q802T2 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 66FD4727DEC9A99F	FASTA	507	57,437
	10 20 30 40 50 60 MEKWSLMTIT VLLALTVRWT VSLGSYSGAG KPPMYGDYEA QRHWQEVTYN LPIRQWYFNT 70 80 90 100 110 120 SDNNLLYWGL DYPPLTAYHS FLCAYVAKLI NPDWIALHTS RGYESQSHKL FMRTTVFVAD 130 140 150 160 170 180 LLIYIPAVIL YCCSLKETST KKKVSSALCI LLYPGLILID HGHFQYNSVS LGFALWGVLC 190 200 210 220 230 240 LSYDWDLLGS AAFCLALNYK QMELYHSLPF FCYLLGKCFK KGLKGKGLLL LIKLAGTVVA 250 260 270 280 290 300 SFAVCWLPFC TDVEQIMQVL RRLFPIDRGL FEDKVANIWC SLSVLIKIKN VVSPQTQLKL 310 320 330 340 350 360 SFAVTFLSLL PTCIKLTVQP SLRGFKLTLV SCALSFFLFS FQVHEKSILL VSVPVCLIIN 370 380 390 400 410 420 EVPFMATWFL LVSTFSMLPL LLKDGLLLPY AVTTLAFLSA CVASFAIFEK TSAKDLQLKP 430 440 450 460 470 480 FSQSLRGYVS WFKLFPKIVR SLFLLSVTLM GVLSVMSAAV HPPQRFPDLF PVSVSSISCL 490 500 HFLFFLVYFN VIILWDSKNS RNQKKVS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel function for mannosyltransferases." Oriol R., Martinez-Duncker R.I., Mollicone R., Chantret I., Codogno P. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:R6.1-R6.9(2005) [PubMed: 15642098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: CB. Tissue: Bursa of Fabricius.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ535824 mRNA. Translation: CAD60191.1. AJ719772 mRNA. Translation: CAG31431.1.
IPI	IPI00822734.
RefSeq	NP_989766.1. NM_204435.1.
UniGene	Gga.5118.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q802T2.
Protein family/group databases
CAZy	GT57. Glycosyltransferase Family 57.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	395078.
KEGG	gga:395078.
Organism-specific databases
CTD	29929.
Phylogenomic databases
eggNOG	veNOG04513.
GeneTree	ENSGT00550000075073.
HOVERGEN	HBG024331.
PhylomeDB	Q802T2.
Family and domain databases
InterPro	IPR004856. Glyco_trans_ALG6/ALG8. [Graphical view]
KO	K03848.
PANTHER	PTHR12413. Alg6_Alg8. 1 hit.
Pfam	PF03155. Alg6_Alg8. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALG6_CHICK

Accession

Primary (citable) accession number: Q802T2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 17, 2007
Last sequence update:	June 1, 2003
Last modified:	November 16, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program