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Q800H9 (H6ST2_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Heparan-sulfate 6-O-sulfotransferase 2

Short name=HS 6-OST-2
Short name=HS6ST-2
EC=2.8.2.-
Gene names
Name:hs6st2
Synonyms:hs6st
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length468 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate. Required for muscle development and angiogenesis. Ref.1 Ref.2

Catalytic activity

3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 6-sulfate.

Subcellular location

Membrane; Single-pass type II membrane protein Potential.

Tissue specificity

Expressed ubiquitously during gastrulation. During early somitogenesis, strong expression in head and presumptive brain. During mid-somitogenesis, strong expression in eye, hindbrain and somitic boundaries and weak expression in tail bud. During late somitogenesis, strong expression in eye, hindbrain, branchial arch primordia, spinal cord and ventral medial somites. At 24 hours post-fertilization (hpf), strong expression throughout the head, with expression receeding from the trunk spinal cord, ventral medial somites and somitic boundaries; expressed in cells surrounding vascular structures of the dorsal aorta and caudal vein in the tail. At 36 hpf, expressed in lens, optic stalk, hindbrain and pectoral fin. At 48 hpf, expressed in eye, brain, otic vesicle and branchial arches. Ref.1 Ref.2 Ref.3

Developmental stage

Expressed both maternally and zygotically throughout the cleavage, gastrulation and somitogenesis stages. Ref.2 Ref.3

Disruption phenotype

Morpholino knockdown results in defects in the branching morphogenesis of the caudal vein and defects in somite specification with impaired muscle differentiation. Ref.1 Ref.2

Sequence similarities

Belongs to the sulfotransferase 6 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 468468Heparan-sulfate 6-O-sulfotransferase 2
PRO_0000190808

Regions

Topological domain1 – 99Cytoplasmic Potential
Transmembrane10 – 3021Helical; Signal-anchor for type II membrane protein; Potential
Topological domain31 – 468438Lumenal Potential
Region106 – 11275'-phosphosulfate-binding Potential
Region197 – 20593'-phosphate binding Potential

Amino acid modifications

Glycosylation791N-linked (GlcNAc...) Potential
Glycosylation2761N-linked (GlcNAc...) Potential
Glycosylation3321N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict1061K → R in AAH45453. Ref.4
Sequence conflict2411D → G in AAH45453. Ref.4
Sequence conflict4341R → Q in CAD44529. Ref.1
Sequence conflict4341R → Q in AAT80868. Ref.2
Sequence conflict4341R → Q in ABH11457. Ref.3
Sequence conflict4611L → S in CAD44529. Ref.1
Sequence conflict4611L → S in AAT80868. Ref.2
Sequence conflict4611L → S in ABH11457. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q800H9 [UniParc].

Last modified March 6, 2007. Version 2.
Checksum: 7E6771A7C818FF0C

FASTA46854,605
        10         20         30         40         50         60 
MDGKSNYSRL LIALLMILFF GGIVLQYICS TSDWQLLHLA SLSSRLGSRA PGDRLNGAGA 

        70         80         90        100        110        120 
GDPYSSEDGA LVRFVPRFNF TTKDLSRAVD FHIKGDDVIV FLHIQKTGGT TFGRHLVRNI 

       130        140        150        160        170        180 
QLERPCECHA GQKKCTCYRP GKRDTWLFSR FSTGWSCGLH ADWTELTNCV PSFMSNRESQ 

       190        200        210        220        230        240 
ERRMTPSRNY YYITILRDPV WRYLSEWRHV QRGATWKASK HMCDGRLPTL TELPSCYPGD 

       250        260        270        280        290        300 
DWSGCSLEEF MVCPYNLANN RQTRMLADLS LVGCYNLTVM SENQRWAMLL ESAKRNLRNM 

       310        320        330        340        350        360 
AFFGLTEYQR KTQYLFEHTF RLSFIAPFTQ LNGTRAASVE VEPETQRRIR ELNQWDVELY 

       370        380        390        400        410        420 
EYARDLFLQR FQFARQQERR EARQRRIQER RKLRAKVKSW LGVTGKAVFK PTKEPPMTEQ 

       430        440        450        460 
SPAFAEEKQA DAERTLESET EGQVEENWLE EDDGEIMLDY LENVEQWR 

« Hide

References

« Hide 'large scale' references
[1]"Heparan sulfate 6-o-sulfotransferase is essential for muscle development in zebrafish."
Bink R.J., Habuhi H., Lele Z., Dolk E., Joore J., Rauch G.-J., Geisler R., Wilson S.W., de Hertog J., Kimata K., Zivkovic D.
J. Biol. Chem. 278:31118-31127(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[2]"A unique role for 6-O sulfation modification in zebrafish vascular development."
Chen E., Stringer S.E., Rusch M.A., Selleck S.B., Ekker S.C.
Dev. Biol. 284:364-376(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[3]"Combinatorial expression patterns of heparan sulfate sulfotransferases in zebrafish: II. The 6-O-sulfotransferase family."
Cadwallader A.B., Yost H.J.
Dev. Dyn. 235:3432-3437(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
[4]NIH - Zebrafish Gene Collection (ZGC) project
Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: AB.
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ505990 mRNA. Translation: CAD44529.1.
AY575769 mRNA. Translation: AAT80868.1.
DQ812995 mRNA. Translation: ABH11457.1.
BC045453 mRNA. Translation: AAH45453.1.
BC065683 mRNA. Translation: AAH65683.1.
RefSeqNP_919402.1. NM_194421.1.
UniGeneDr.77382.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSDART00000002250; ENSDARP00000022497; ENSDARG00000012025.
GeneID378450.
KEGGdre:378450.

Organism-specific databases

CTD90161.
ZFINZDB-GENE-030909-14. hs6st2.

Phylogenomic databases

eggNOGNOG302961.
GeneTreeENSGT00390000013468.
HOGENOMHOG000007772.
HOVERGENHBG083012.
InParanoidQ800H9.
KOK08102.
OMAKRNLRGM.
OrthoDBEOG75MVW3.
TreeFamTF312835.

Gene expression databases

BgeeQ800H9.

Family and domain databases

InterProIPR010635. Heparan_SO4-6-sulfoTrfase.
IPR027417. P-loop_NTPase.
IPR005331. Sulfotransferase.
[Graphical view]
PANTHERPTHR12812. PTHR12812. 1 hit.
PfamPF03567. Sulfotransfer_2. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
ProtoNetSearch...

Other

NextBio20813773.

Entry information

Entry nameH6ST2_DANRE
AccessionPrimary (citable) accession number: Q800H9
Secondary accession number(s): A0MGZ6, Q6P0B4, Q7ZVQ5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: March 6, 2007
Last modified: February 19, 2014
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families