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Q800H9

- H6ST2_DANRE

UniProt

Q800H9 - H6ST2_DANRE

Protein

Heparan-sulfate 6-O-sulfotransferase 2

Gene

hs6st2

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 2 (06 Mar 2007)
      Previous versions | rss
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    Functioni

    6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate. Required for muscle development and angiogenesis.2 Publications

    Catalytic activityi

    3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 6-sulfate.

    GO - Molecular functioni

    1. heparan sulfate 6-O-sulfotransferase activity Source: ZFIN

    GO - Biological processi

    1. cell differentiation Source: UniProtKB-KW
    2. embryonic eye morphogenesis Source: ZFIN
    3. metabolic process Source: GOC
    4. muscle organ development Source: UniProtKB-KW
    5. patterning of blood vessels Source: ZFIN

    Keywords - Molecular functioni

    Developmental protein, Transferase

    Keywords - Biological processi

    Angiogenesis, Differentiation, Myogenesis

    Enzyme and pathway databases

    ReactomeiREACT_226880. HS-GAG biosynthesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Heparan-sulfate 6-O-sulfotransferase 2 (EC:2.8.2.-)
    Short name:
    HS 6-OST-2
    Short name:
    HS6ST-2
    Gene namesi
    Name:hs6st2
    Synonyms:hs6st
    OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
    Taxonomic identifieri7955 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
    ProteomesiUP000000437: Chromosome 14

    Organism-specific databases

    ZFINiZDB-GENE-030909-14. hs6st2.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Morpholino knockdown results in defects in the branching morphogenesis of the caudal vein and defects in somite specification with impaired muscle differentiation.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 468468Heparan-sulfate 6-O-sulfotransferase 2PRO_0000190808Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi276 – 2761N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi332 – 3321N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Tissue specificityi

    Expressed ubiquitously during gastrulation. During early somitogenesis, strong expression in head and presumptive brain. During mid-somitogenesis, strong expression in eye, hindbrain and somitic boundaries and weak expression in tail bud. During late somitogenesis, strong expression in eye, hindbrain, branchial arch primordia, spinal cord and ventral medial somites. At 24 hours post-fertilization (hpf), strong expression throughout the head, with expression receeding from the trunk spinal cord, ventral medial somites and somitic boundaries; expressed in cells surrounding vascular structures of the dorsal aorta and caudal vein in the tail. At 36 hpf, expressed in lens, optic stalk, hindbrain and pectoral fin. At 48 hpf, expressed in eye, brain, otic vesicle and branchial arches.3 Publications

    Developmental stagei

    Expressed both maternally and zygotically throughout the cleavage, gastrulation and somitogenesis stages.2 Publications

    Gene expression databases

    BgeeiQ800H9.

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 99CytoplasmicSequence Analysis
    Topological domaini31 – 468438LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei10 – 3021Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni106 – 11275'-phosphosulfate-bindingSequence Analysis
    Regioni197 – 20593'-phosphate bindingSequence Analysis

    Sequence similaritiesi

    Belongs to the sulfotransferase 6 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG302961.
    GeneTreeiENSGT00390000013468.
    HOGENOMiHOG000007772.
    HOVERGENiHBG083012.
    InParanoidiQ800H9.
    KOiK08102.
    OMAiKRNLRGM.
    OrthoDBiEOG75MVW3.
    PhylomeDBiQ800H9.
    TreeFamiTF312835.

    Family and domain databases

    InterProiIPR010635. Heparan_SO4-6-sulfoTrfase.
    IPR027417. P-loop_NTPase.
    IPR005331. Sulfotransferase.
    [Graphical view]
    PANTHERiPTHR12812. PTHR12812. 1 hit.
    PfamiPF03567. Sulfotransfer_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q800H9-1 [UniParc]FASTAAdd to Basket

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    MDGKSNYSRL LIALLMILFF GGIVLQYICS TSDWQLLHLA SLSSRLGSRA    50
    PGDRLNGAGA GDPYSSEDGA LVRFVPRFNF TTKDLSRAVD FHIKGDDVIV 100
    FLHIQKTGGT TFGRHLVRNI QLERPCECHA GQKKCTCYRP GKRDTWLFSR 150
    FSTGWSCGLH ADWTELTNCV PSFMSNRESQ ERRMTPSRNY YYITILRDPV 200
    WRYLSEWRHV QRGATWKASK HMCDGRLPTL TELPSCYPGD DWSGCSLEEF 250
    MVCPYNLANN RQTRMLADLS LVGCYNLTVM SENQRWAMLL ESAKRNLRNM 300
    AFFGLTEYQR KTQYLFEHTF RLSFIAPFTQ LNGTRAASVE VEPETQRRIR 350
    ELNQWDVELY EYARDLFLQR FQFARQQERR EARQRRIQER RKLRAKVKSW 400
    LGVTGKAVFK PTKEPPMTEQ SPAFAEEKQA DAERTLESET EGQVEENWLE 450
    EDDGEIMLDY LENVEQWR 468
    Length:468
    Mass (Da):54,605
    Last modified:March 6, 2007 - v2
    Checksum:i7E6771A7C818FF0C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti106 – 1061K → R in AAH45453. 1 PublicationCurated
    Sequence conflicti241 – 2411D → G in AAH45453. 1 PublicationCurated
    Sequence conflicti434 – 4341R → Q in CAD44529. (PubMed:12782624)Curated
    Sequence conflicti434 – 4341R → Q in AAT80868. (PubMed:16009360)Curated
    Sequence conflicti434 – 4341R → Q in ABH11457. (PubMed:17075883)Curated
    Sequence conflicti461 – 4611L → S in CAD44529. (PubMed:12782624)Curated
    Sequence conflicti461 – 4611L → S in AAT80868. (PubMed:16009360)Curated
    Sequence conflicti461 – 4611L → S in ABH11457. (PubMed:17075883)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ505990 mRNA. Translation: CAD44529.1.
    AY575769 mRNA. Translation: AAT80868.1.
    DQ812995 mRNA. Translation: ABH11457.1.
    BC045453 mRNA. Translation: AAH45453.1.
    BC065683 mRNA. Translation: AAH65683.1.
    RefSeqiNP_919402.1. NM_194421.1.
    UniGeneiDr.77382.

    Genome annotation databases

    EnsembliENSDART00000002250; ENSDARP00000022497; ENSDARG00000012025.
    GeneIDi378450.
    KEGGidre:378450.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ505990 mRNA. Translation: CAD44529.1 .
    AY575769 mRNA. Translation: AAT80868.1 .
    DQ812995 mRNA. Translation: ABH11457.1 .
    BC045453 mRNA. Translation: AAH45453.1 .
    BC065683 mRNA. Translation: AAH65683.1 .
    RefSeqi NP_919402.1. NM_194421.1.
    UniGenei Dr.77382.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSDART00000002250 ; ENSDARP00000022497 ; ENSDARG00000012025 .
    GeneIDi 378450.
    KEGGi dre:378450.

    Organism-specific databases

    CTDi 90161.
    ZFINi ZDB-GENE-030909-14. hs6st2.

    Phylogenomic databases

    eggNOGi NOG302961.
    GeneTreei ENSGT00390000013468.
    HOGENOMi HOG000007772.
    HOVERGENi HBG083012.
    InParanoidi Q800H9.
    KOi K08102.
    OMAi KRNLRGM.
    OrthoDBi EOG75MVW3.
    PhylomeDBi Q800H9.
    TreeFami TF312835.

    Enzyme and pathway databases

    Reactomei REACT_226880. HS-GAG biosynthesis.

    Miscellaneous databases

    NextBioi 20813773.

    Gene expression databases

    Bgeei Q800H9.

    Family and domain databases

    InterProi IPR010635. Heparan_SO4-6-sulfoTrfase.
    IPR027417. P-loop_NTPase.
    IPR005331. Sulfotransferase.
    [Graphical view ]
    PANTHERi PTHR12812. PTHR12812. 1 hit.
    Pfami PF03567. Sulfotransfer_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Heparan sulfate 6-o-sulfotransferase is essential for muscle development in zebrafish."
      Bink R.J., Habuhi H., Lele Z., Dolk E., Joore J., Rauch G.-J., Geisler R., Wilson S.W., de Hertog J., Kimata K., Zivkovic D.
      J. Biol. Chem. 278:31118-31127(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
    2. "A unique role for 6-O sulfation modification in zebrafish vascular development."
      Chen E., Stringer S.E., Rusch M.A., Selleck S.B., Ekker S.C.
      Dev. Biol. 284:364-376(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
    3. "Combinatorial expression patterns of heparan sulfate sulfotransferases in zebrafish: II. The 6-O-sulfotransferase family."
      Cadwallader A.B., Yost H.J.
      Dev. Dyn. 235:3432-3437(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
    4. NIH - Zebrafish Gene Collection (ZGC) project
      Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: AB.
      Tissue: Embryo.

    Entry informationi

    Entry nameiH6ST2_DANRE
    AccessioniPrimary (citable) accession number: Q800H9
    Secondary accession number(s): A0MGZ6, Q6P0B4, Q7ZVQ5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2005
    Last sequence update: March 6, 2007
    Last modified: October 1, 2014
    This is version 70 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3