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Protein

Disintegrin jerdostatin

Gene
N/A
Organism
Protobothrops jerdonii (Jerdon's pitviper) (Trimeresurus jerdonii)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Recombinant protein inhibits the adhesion of alpha-1/beta-1-K562 (ITGA1/ITGB1) cells to collagen IV with an IC50 of 80 nM.2 Publications

Miscellaneous

Does not show inhibitory activity toward alpha-IIb/beta-3 (ITGA2B/ITGB3), alpha-V/beta-3 (ITGAV/ITGB3), alpha-2/beta-1 (ITGA2/ITGB1), alpha-5/beta-1 (ITGA5/ITGB1), alpha-4/beta-1 (ITGA4/ITGB1), alpha-6/beta-1 (ITGA6/ITGB1), and alpha-9/beta-1 (ITGA9/ITGB1).1 Publication

GO - Molecular functioni

Keywordsi

Molecular functionCell adhesion impairing toxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Disintegrin jerdostatin
OrganismiProtobothrops jerdonii (Jerdon's pitviper) (Trimeresurus jerdonii)
Taxonomic identifieri242841 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeProtobothrops

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi88R → K: Decrease in inhibitory activity towards ITGA1/ITGB1 integrin (IC(50) is 300 nM). Decrease in inhibitory activity towards ITGA1/ITGB1 integrin (IC(50) of 703 nM) (IC(50) is 703 nM); when associated with G-108. 2 Publications1
Mutagenesisi108G → GNG: Decrease in inhibitory activity towards ITGA1/ITGB1 integrin (IC(50) is 180 nM). Decrease in inhibitory activity towards ITGA1/ITGB1 integrin (IC(50) is 703 nM); when associated with R-88. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
PropeptideiPRO_000032260321 – 67By similarityAdd BLAST47
ChainiPRO_000000724668 – 108Disintegrin jerdostatinAdd BLAST41
PropeptideiPRO_0000424722109 – 110By similarity2

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi68 ↔ 771 Publication
Disulfide bondi73 ↔ 961 Publication
Disulfide bondi74 ↔ 1011 Publication
Disulfide bondi86 ↔ 1031 Publication

Post-translational modificationi

Two conformers are found, they may differ by their disulfide bond connectivities. Conformer 2 is 33 times less active than conformer 1. Conformer 2 may represent a non-native protein (PubMed:16215260).1 Publication
The C-terminal dipeptide may be post-translationally removed, as seen in disintegrins that possess a KTS integrin-binding motif.

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

Secondary structure

1110
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi71 – 77Combined sources7
Beta strandi85 – 87Combined sources3
Beta strandi89 – 91Combined sources3
Beta strandi92 – 95Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2W9ONMR-A68-110[»]
2W9UNMR-A68-110[»]
2W9VNMR-A68-108[»]
2W9WNMR-A68-108[»]
ProteinModelPortaliQ7ZZM2.
SMRiQ7ZZM2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7ZZM2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 110DisintegrinAdd BLAST84

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi88 – 90Cell attachment site; atypical (RTS)3

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG051359.

Family and domain databases

Gene3Di4.10.70.10. 1 hit.
InterProiView protein in InterPro
IPR036436. Disintegrin_dom_sf.
SUPFAMiSSF57552. SSF57552. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7ZZM2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIQVLLVTIC LAVFPYQVSS KTLKSGSVNE YEVVNPGTVT GLPKGAVKQP
60 70 80 90 100
EKKHEPMKGN TLQKLPLCTT GPCCRQCKLK PAGTTCWRTS VSSHYCTGRS
110
CECPSYPGNG
Length:110
Mass (Da):11,849
Last modified:June 1, 2003 - v1
Checksum:iC14C5D7986C9F5E7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY262730 mRNA. Translation: AAP20878.1.

Similar proteinsi

Entry informationi

Entry nameiDIS_PROJR
AccessioniPrimary (citable) accession number: Q7ZZM2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 1, 2003
Last modified: November 22, 2017
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Caution

The mature sequence shown is shorter than the sequence proposed in PubMed:16215260 and PubMed:21656569. A comparison of jerdostatin with KTS-disintegrins and a higher potency (IC50 of 80 nM compared to 180 nM) suggest that the C-terminal dipeptide is post-translationally removed.Curated

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families