Q7ZY31 (SPRE_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sepiapterin reductase
Short name=SPR
EC=1.1.1.153

Gene names

Name:

spr

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the final one or two reductions in tetra-hydrobiopterin biosynthesis to form 5,6,7,8-tetrahydrobiopterin By similarity.
Catalytic activity	L-erythro-7,8-dihydrobiopterin + NADP+ = sepiapterin + NADPH. L-erythro-tetrahydrobiopterin + 2 NADP+ = 6-pyruvoyl-5,6,7,8-tetrahydropterin + 2 NADPH.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the sepiapterin reductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	tetrahydrobiopterin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sepiapterin reductase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 263	263	Sepiapterin reductase		PRO_0000327643
Regions
	Nucleotide binding	18 – 24	7	NADP By similarity
	Nucleotide binding	46 – 47	2	NADP By similarity
	Nucleotide binding	73 – 74	2	NADP By similarity
	Nucleotide binding	204 – 209	6	NADP By similarity
	Region	160 – 161	2	Substrate binding By similarity
Sites
	Binding site	173	1	Substrate By similarity
	Binding site	177	1	NADP By similarity
	Binding site	202	1	Substrate; via amide nitrogen By similarity
	Binding site	260	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7ZY31 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 5B20B26508DAADDD	FASTA	263	28,706
	10 20 30 40 50 60 MTAARAGALG SVLCVLTGAS RGFGRTLAHE LCPRVLPGST LLLVSRTEEA LKGLAEELGH 70 80 90 100 110 120 EFPGVRVRWA AADLSTTEGV SATVRAAREL QAGTAHRLLI INNAGSIGDV SKMFVDFSAP 130 140 150 160 170 180 EEVTEYMKFN VSSPLCLTAS LLKTFPRRPD LQRLVVNVSS LAALQPYKSW VLYCSGKAAR 190 200 210 220 230 240 DMMFRVLAEE EDDVRVLSYA PGPLDTDMHE VACTQTADPE LRRAIMDRKE KGNMVDIRVS 250 260 ANKMLDLLEA DAYKSGDHID FYD

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC043999 mRNA. Translation: AAH43999.1.
RefSeq	NP_001080581.1. NM_001087112.1.
UniGene	Xl.27670.
3D structure databases
ProteinModelPortal	Q7ZY31.
SMR	Q7ZY31. Positions 6-263.
ModBase	Search...
Proteomic databases
PRIDE	Q7ZY31.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	380273.
KEGG	xla:380273.
Organism-specific databases
CTD	6697.
Xenbase	XB-GENE-6253916. spr.
Phylogenomic databases
HOVERGEN	HBG006973.
KO	K00072.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR006393. Sepiapterin_red. [Graphical view]
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH.
TIGRFAMs	TIGR01500. sepiapter_red. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SPRE_XENLA

Accession

Primary (citable) accession number: Q7ZY31

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	June 1, 2003
Last modified:	May 29, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program