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Protein

Zygotic DNA replication licensing factor mcm6-B

Gene

zmcm6-b

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as component of the mcm2-7 complex (mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. The existence of maternal and zygotic forms of mcm3 and mcm6 suggests that specific forms of mcm2-7 complexes may be used during different stages of development. May replace mmcm6 in the mcm2-7 complex.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri159 – 18628C4-typeSequence analysisAdd
BLAST
Nucleotide bindingi397 – 4048ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • DNA replication initiation Source: InterPro
  • regulation of DNA-dependent DNA replication initiation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Cell cycle, DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zygotic DNA replication licensing factor mcm6-B (EC:3.6.4.12)
Alternative name(s):
Zygotic minichromosome maintenance protein 6-B
Short name:
zMCM6-B
Short name:
zMCM6b
Gene namesi
Name:zmcm6-b
Synonyms:zmcm6b1 Publication
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6256556. mcm6.2.

Subcellular locationi

  • Nucleus By similarity

  • Note: Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses.By similarity

GO - Cellular componenti

  • MCM complex Source: UniProtKB
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 825825Zygotic DNA replication licensing factor mcm6-BPRO_0000235885Add
BLAST

Proteomic databases

PRIDEiQ7ZY18.

Expressioni

Developmental stagei

Expressed zygotically. Expression begins after the midblastula transition (MBT) at the neurula stage.1 Publication

Interactioni

Subunit structurei

Component of the mcm2-7 complex (RLF-M). The complex forms a toroidal hexameric ring with the proposed subunit order mcm2-mcm6-mcm4-mcm7-mcm3-mcm5 (By simililarity). Begins to associate with zmcm3, mcm4 and mcm7 into mcm complexes at the neurula stage.1 Publication

Protein-protein interaction databases

IntActiQ7ZY18. 4 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ7ZY18.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini347 – 554208MCMSequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi529 – 5324Arginine finger

Sequence similaritiesi

Belongs to the MCM family.Sequence analysis
Contains 1 MCM domain.Sequence analysis

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri159 – 18628C4-typeSequence analysisAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOVERGENiHBG006334.
KOiK02542.

Family and domain databases

Gene3Di2.20.28.10. 1 hit.
2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR031327. MCM.
IPR008049. MCM6.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
IPR004039. Rubredoxin-type_fold.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01662. MCMPROTEIN6.
SMARTiSM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7ZY18-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLVDPSQSA AAAAGTQLVK DEVAEKCQKL FQDFLEEFRG SDGELKYQSD
60 70 80 90 100
AEELIRPERN TLLVSFVDLE QFNQQLATTI QEEFYRVYPY LCRAVKAFAR
110 120 130 140 150
DHGNIPQNKE FYVAFQELPT RHKIRELTTP RIGSLLRISG QVVRTHPVHP
160 170 180 190 200
ELVSGTFLCL DCQTLVRDVE QQFKYTQPSI CRNPVCANRK RFMLDTNKSR
210 220 230 240 250
FVDFQKVRIQ ETQAELPRGS IPRSVEVILR AEAVESCQAG DRCDFTGSLI
260 270 280 290 300
VVPDISQLST PGVRAETSSR VGGREGYEAE GVQGLRALGV RDLSYKLVFL
310 320 330 340 350
ACYVCPTNPR FGGKDLHEED MTAESIKNQM SVKEWEKVFE MSQDKNLYHN
360 370 380 390 400
LCTSLFPTVH GNDEVKRGIL LMLFGGVPKS TMEGTSLRGD INVCVVGDPS
410 420 430 440 450
TAKSQFLKHV EEFSPRAVYT SGKASTAAGL TAAVVRDEES HEFVIEAGAL
460 470 480 490 500
MLADNGVCCI DEFDKMDTKD QVAIHEAMEQ QTISITKAGV KATLNARTSI
510 520 530 540 550
LAAANPVGGR YDRAKSLKQN VNLSAPIMSR FDLFFILVDE CNEVTDYAIA
560 570 580 590 600
RRIVDLHSRI EESIDRVYTL DEVRRYLLFA RQFKPKISKE SEDFIVEQYK
610 620 630 640 650
RLRQRDGSGV TKSAWRITVR QLESMIRLSE GMARMHCSDE VQPKHVKEAF
660 670 680 690 700
RLLNKSIIRV ETPDVNLDQE DEHEVEEPQE GINGDADVPN GVNGHINGIN
710 720 730 740 750
GHAEETNAAP PKPSLRLNFA EYKRISNLLV LQLRKIEDED DENETSQRKS
760 770 780 790 800
ELINWYLKEI ESEIDSEEEL VNRKQIIDKV IHRLVHYDQI LIELIQTGLK
810 820
GTEDENVAKE EDPYLVVNPN YILED
Length:825
Mass (Da):93,378
Last modified:June 1, 2003 - v1
Checksum:iA96CF39A8BE3FDF4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti550 – 5501A → T in AAC41267 (PubMed:9512418).Curated
Sequence conflicti581 – 5811R → K in AAC41267 (PubMed:9512418).Curated
Sequence conflicti793 – 7931E → A in AAC41267 (PubMed:9512418).Curated
Sequence conflicti810 – 8101Missing in AAC41267 (PubMed:9512418).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031139 mRNA. Translation: AAC41267.1.
BC044019 mRNA. Translation: AAH44019.1.
RefSeqiNP_001080590.1. NM_001087121.1.
UniGeneiXl.7195.

Genome annotation databases

GeneIDi380282.
KEGGixla:380282.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031139 mRNA. Translation: AAC41267.1.
BC044019 mRNA. Translation: AAH44019.1.
RefSeqiNP_001080590.1. NM_001087121.1.
UniGeneiXl.7195.

3D structure databases

ProteinModelPortaliQ7ZY18.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ7ZY18. 4 interactions.

Proteomic databases

PRIDEiQ7ZY18.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi380282.
KEGGixla:380282.

Organism-specific databases

CTDi380282.
XenbaseiXB-GENE-6256556. mcm6.2.

Phylogenomic databases

HOVERGENiHBG006334.
KOiK02542.

Family and domain databases

Gene3Di2.20.28.10. 1 hit.
2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR031327. MCM.
IPR008049. MCM6.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
IPR004039. Rubredoxin-type_fold.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01662. MCMPROTEIN6.
SMARTiSM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Developmental regulation of MCM replication factors in Xenopus laevis."
    Sible J.C., Erikson E., Hendrickson M., Maller J.L., Gautier J.
    Curr. Biol. 8:347-350(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION IN A COMPLEX WITH ZMCM3; MCM4 AND MCM7, DEVELOPMENTAL STAGE.
    Tissue: Embryo1 Publication.
  2. NIH - Xenopus Gene Collection (XGC) project
    Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: EmbryoImported.

Entry informationi

Entry nameiMC6ZB_XENLA
AccessioniPrimary (citable) accession number: Q7ZY18
Secondary accession number(s): O73710
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: June 1, 2003
Last modified: November 11, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.