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Q7ZXR7 (UB10B_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 10-B

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 10-B
Ubiquitin thioesterase 10-B
Ubiquitin-specific-processing protease 10-B
Gene names
Name:usp10-b
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length805 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/tp53. Acts as an essential regulator of p53/tp53 stability: in unstressed cells, specifically deubiquitinates p53/tp53 in the cytoplasm, leading to counteracts MDM2 action and stabilize p53/tp53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/tp53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/tp53 levels By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Sequence similarities

Belongs to the peptidase C19 family. USP10 subfamily.

Contains 1 USP domain.

Ontologies

Keywords
   Biological processAutophagy
DNA damage
DNA repair
Ubl conjugation pathway
   Cellular componentCytoplasm
Nucleus
   Molecular functionHydrolase
Protease
Thiol protease
Gene Ontology (GO)
   Biological_processDNA damage response, signal transduction by p53 class mediator

Inferred from sequence or structural similarity. Source: UniProtKB

DNA repair

Inferred from electronic annotation. Source: UniProtKB-KW

autophagy

Inferred from electronic annotation. Source: UniProtKB-KW

protein deubiquitination

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of autophagy

Inferred from sequence or structural similarity. Source: UniProtKB

ubiquitin-dependent protein catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

early endosome

Inferred from sequence or structural similarity. Source: UniProtKB

nucleus

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functioncysteine-type endopeptidase activity

Inferred from sequence or structural similarity. Source: UniProtKB

ion channel binding

Inferred from sequence or structural similarity. Source: UniProtKB

p53 binding

Inferred from sequence or structural similarity. Source: UniProtKB

ubiquitin thiolesterase activity

Inferred from sequence or structural similarity. Source: UniProtKB

ubiquitin-specific protease activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 805805Ubiquitin carboxyl-terminal hydrolase 10-B
PRO_0000393003

Regions

Domain422 – 802381USP

Sites

Active site4311Nucleophile By similarity
Active site7561Proton acceptor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7ZXR7 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: FEC173B9A8021C2E

FASTA80589,197
        10         20         30         40         50         60 
MASPSGQYIF GEFSDDEFKQ FFVTARCTVE LPPYNEHFFP CGPQSSVDFQ DDMHLKFSEV 

        70         80         90        100        110        120 
IHGISGEECP RIEFGIEQVV DRDTALNNNT DYSISSNLNP QAPEFILTCS SFPKTSNNVL 

       130        140        150        160        170        180 
HENNFDAINC QFSESAIPDG SGNADSDGTS GTGQRERKKK KKRPPGYYSY LEGVGDVPSE 

       190        200        210        220        230        240 
TLVNGHANST GLDSISTDDP DLADDIPIST TSPRTCTSPD NFVDLINEAL SDEASMHNVL 

       250        260        270        280        290        300 
DNARTAGQPE ECSVTSSEQS CIPSDNGSES PVRTAVVQPF AGTDTTENLG VTNGQTLESP 

       310        320        330        340        350        360 
EEDTVSNGVV LHPEVSSFSE EVKTEETSTA QALIHLSGSA SSNPPAKSWA SLFHTSKPSS 

       370        380        390        400        410        420 
SPQVAYVETK NAPTVVSPQV PEKQVEIKEE PVPVSDDPVA IEFAELLEEV KLVHKPVSLQ 

       430        440        450        460        470        480 
PRGLINKGNW CYINATLQAL VACPPMYHLM KSIPVYTKAQ RPCTSTPMID SFVRLMNEFT 

       490        500        510        520        530        540 
NMPILPKAKQ APGEKVIKDI RPGAPFEPTY IYRLLTVFKS SLSEKGRQED AEEYLGFILN 

       550        560        570        580        590        600 
GLHEEMLALK KLLLPQNDQI HINNCPNPVS GVEEVNKEEQ EGSDEEWEQV GPRNKSSVTR 

       610        620        630        640        650        660 
QADFVQTPIT DIFGGHMRSV VYQQSSKESA TLQPFFTLQL DIQSEKIRTV QDALESLVAR 

       670        680        690        700        710        720 
ESVQGYTTKT KQEVEICRRV TLEELPPVLV LHLKRFVFEK TGGCQKLIKN IEYPVDLEVS 

       730        740        750        760        770        780 
KDLLSPGVKS KIFKGQRTYR LFAVVYHHGN SATGGHYTTD VFQIGLNGWL RIDDQSVKVI 

       790        800 
NQYQVVKQTV ERTAYLLYYR RVDLL 

« Hide

References

[1]NIH - Xenopus Gene Collection (XGC) project
Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Embryo.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC044285 mRNA. Translation: AAH44285.1.
RefSeqNP_001080643.1. NM_001087174.1.
UniGeneXl.2271.

3D structure databases

ProteinModelPortalQ7ZXR7.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC19.018.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID380335.
KEGGxla:380335.

Organism-specific databases

CTD9100.

Phylogenomic databases

HOVERGENHBG059823.
KOK11841.

Family and domain databases

InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR028767. USP10.
[Graphical view]
PANTHERPTHR24006:SF69. PTHR24006:SF69. 1 hit.
PfamPF00443. UCH. 1 hit.
[Graphical view]
PROSITEPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUB10B_XENLA
AccessionPrimary (citable) accession number: Q7ZXR7
Entry history
Integrated into UniProtKB/Swiss-Prot: March 23, 2010
Last sequence update: June 1, 2003
Last modified: April 16, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries