Q7ZX21 (OTU5A_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
OTU domain-containing protein 5-A
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme A
Short name=DUBA

Gene names

Name:

otud5-a

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	513 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Deubiquitinating enzyme that may function as negative regulator of the innate immune system. Has peptidase activity towards 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Can also cleave 'Lys-11'-linked ubiquitin chains (in vitro) By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Sequence similarities	Belongs to the peptidase C85 family. Contains 1 OTU domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Hydrolase Protease Thiol protease
Gene Ontology (GO)
Biological_process	protein K48-linked deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein K63-linked deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB proteolysis Inferred from electronic annotation. Source: UniProtKB-KW response to lipopolysaccharide Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ubiquitin-specific protease activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 513	513	OTU domain-containing protein 5-A		PRO_0000278227
Regions
	Domain	166 – 289	124	OTU
Sites
	Active site	174	1	Potential
	Active site	177	1	Nucleophile By similarity
	Active site	282	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7ZX21 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: A8734AD6E686FEDA	FASTA	513	55,868
	10 20 30 40 50 60 MTILPKKKPP PPPDPEANGE RSGSGAPDSH SRSGARPRSS PPPRWAYPGN PSSAAERHTQ 70 80 90 100 110 120 QVSPPPGSAT SGGAGPLGDG ALGSCCCCNG AGAGGCCSGP GHSKRRRQVL SAGPGATGNC 130 140 150 160 170 180 PDTDDGAGNN SEDEYETAAQ TQHLDPDTAE QQELCFEKTL SDKKGFIIKQ MKEDGACLFR 190 200 210 220 230 240 AVADQVYGDQ DMHEVVRKHC MDYLMKNADY FSNYVTEDFT TYINRKRKNN CHGNHIEMQA 250 260 270 280 290 300 MAEMYNRPVE VYQYGTEPIN TFHGIQKNED EPIRVSYHRN IHYNSVVNPN KATIGVGLGL 310 320 330 340 350 360 PSFKPGYAEQ SLMKSAIRTS EESWIEQQML EDKKRATDWE ATNEAIEEQV ARESYLQWLR 370 380 390 400 410 420 DQEKQARQPR KASATCSSAT AAACSGLEEW SGRSPRQRST AGSPEHPDLH AELCMKPPSP 430 440 450 460 470 480 GAPLILGKPP SPCAPGPSNQ MSTGADRATS PLVSLYPALE CRAIMQHMSP TAFGLKDWDN 490 500 510 DEILASVLAA SQQEYLDTMK KSTLRRESSP DHS

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC046254 mRNA. Translation: AAH46254.1.
RefSeq	NP_001080318.1. NM_001086849.1.
UniGene	Xl.23288.
3D structure databases
ProteinModelPortal	Q7ZX21.
ModBase	Search...
Protein family/group databases
MEROPS	C85.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	380010.
KEGG	xla:380010.
Organism-specific databases
CTD	380010.
Xenbase	XB-GENE-6254232. otud5.
Phylogenomic databases
HOVERGEN	HBG060214.
KO	K12655.
Family and domain databases
InterPro	IPR003323. OTU. [Graphical view]
Pfam	PF02338. OTU. 1 hit. [Graphical view]
PROSITE	PS50802. OTU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTU5A_XENLA

Accession

Primary (citable) accession number: Q7ZX21

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	June 1, 2003
Last modified:	July 11, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program