Q7ZX15 (AKT2A_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RAC-beta serine/threonine-protein kinase A
EC=2.7.11.1

Alternative name(s):
Protein kinase Akt-2-A
Protein kinase B, beta-A
Short name=PKB beta-A
RAC-PK-beta-A

Gene names

Name:

akt2-a

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Akt2-a is one of several closely related serine/threonine-protein kinases known as the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. UniProtKB Q60823
Enzyme regulation	Two specific sites, one in the kinase domain (Thr-314) and the other in the C-terminal regulatory region (Ser-479), need to be phosphorylated for its full activation By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 PH domain. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 486	486	RAC-beta serine/threonine-protein kinase A		PRO_0000223508
Regions
	Domain	5 – 110	106	PH
	Domain	157 – 414	258	Protein kinase
	Domain	415 – 486	72	AGC-kinase C-terminal
	Nucleotide binding	163 – 171	9	ATP By similarity
Sites
	Active site	280	1	Proton acceptor By similarity
	Binding site	186	1	ATP By similarity
Amino acid modifications
	Modified residue	314	1	Phosphothreonine By similarity
	Modified residue	479	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7ZX15 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: F124CAE30758016B	FASTA	486	56,310
	10 20 30 40 50 60 MNEVMVIKEG WLQKRGEYIK TWRPRYFLLK SDGSFIGYKE KPESTEHNVV LPPLNNFSVA 70 80 90 100 110 120 ECQLMKTERP RPNTFVIRCL QWTTVIERTF HVDTPEEREE WIIAIQTVAN GLKNQVPEDE 130 140 150 160 170 180 EEEAMEVKYG SPSDVSSAEQ MDVAMSKGHP KVTMNDFDYL KLLGKGTFGK VILVREKATG 190 200 210 220 230 240 RYYAMKILRK EVIIAKDEVA HTLTESRVLQ NTKHPFLTAL KYAFQTSDRL CFVMEYANGG 250 260 270 280 290 300 ELFFHLSRER VFTEDRARFY GAEIVSALEY LHSRNVVYRD IKLENLMLDK DGHVKITDFG 310 320 330 340 350 360 LCKEGITDGA TMRTFCGTPE YLAPEVLEDN DYGRAVDWWG LGVVMYEMMC GRLPFYNQDH 370 380 390 400 410 420 ERLFELILME EIRFPRTLSP EAKSLLAGLL KKDPKQRLGG GPNDAQEVMS HRFFVSINWQ 430 440 450 460 470 480 DVTERKLTPP FKPQVTSEID TRYFDDEFTA QSITLTPPDR YDNLDALESD QRPHFPQFSY SASIRE

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC046261 mRNA. Translation: AAH46261.1.
RefSeq	NP_001080091.1. NM_001086622.1.
UniGene	Xl.5248.
3D structure databases
ProteinModelPortal	Q7ZX15.
SMR	Q7ZX15. Positions 1-113, 151-484.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	379783.
KEGG	xla:379783.
Organism-specific databases
CTD	208.
Xenbase	XB-GENE-484172. akt2.
Phylogenomic databases
HOVERGEN	HBG108317.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR011009. Kinase-like_dom. IPR011993. PH_type. IPR017892. Pkinase_C. IPR001849. Pleckstrin_homology. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
KO	K04456.
Pfam	PF00169. PH. 1 hit. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view]
SMART	SM00233. PH. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS50003. PH_DOMAIN. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AKT2A_XENLA

Accession

Primary (citable) accession number: Q7ZX15

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2006
Last sequence update:	June 1, 2003
Last modified:	November 16, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program