ID   CISY_XENLA              Reviewed;         468 AA.
AC   Q7ZWZ5;
DT   17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   27-MAR-2024, entry version 90.
DE   RecName: Full=Citrate synthase, mitochondrial;
DE            EC=2.3.3.1;
DE   AltName: Full=Citrate (Si)-synthase;
DE   Flags: Precursor;
GN   Name=cs;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC         Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.1; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU10117};
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC       from oxaloacetate: step 1/2.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000250}.
CC   -!- MISCELLANEOUS: Citrate synthase is found in nearly all cells capable of
CC       oxidative metabolism.
CC   -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
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DR   EMBL; BC046571; AAH46571.1; -; mRNA.
DR   RefSeq; NP_001080194.1; NM_001086725.1.
DR   AlphaFoldDB; Q7ZWZ5; -.
DR   SMR; Q7ZWZ5; -.
DR   DNASU; 379886; -.
DR   GeneID; 379886; -.
DR   KEGG; xla:379886; -.
DR   AGR; Xenbase:XB-GENE-943167; -.
DR   CTD; 379886; -.
DR   Xenbase; XB-GENE-943167; cs.L.
DR   OrthoDB; 3513214at2759; -.
DR   UniPathway; UPA00223; UER00717.
DR   Proteomes; UP000186698; Chromosome 2L.
DR   Bgee; 379886; Expressed in muscle tissue and 19 other cell types or tissues.
DR   GO; GO:0005759; C:mitochondrial matrix; ISS:UniProtKB.
DR   GO; GO:0004108; F:citrate (Si)-synthase activity; ISS:UniProtKB.
DR   GO; GO:0005975; P:carbohydrate metabolic process; ISS:UniProtKB.
DR   GO; GO:0006101; P:citrate metabolic process; IEA:InterPro.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR   CDD; cd06105; ScCit1-2_like; 1.
DR   Gene3D; 1.10.580.10; Citrate Synthase, domain 1; 1.
DR   Gene3D; 1.10.230.10; Cytochrome P450-Terp, domain 2; 1.
DR   InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR   InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR   InterPro; IPR002020; Citrate_synthase.
DR   InterPro; IPR019810; Citrate_synthase_AS.
DR   InterPro; IPR010109; Citrate_synthase_euk.
DR   InterPro; IPR036969; Citrate_synthase_sf.
DR   NCBIfam; TIGR01793; cit_synth_euk; 1.
DR   PANTHER; PTHR11739; CITRATE SYNTHASE; 1.
DR   PANTHER; PTHR11739:SF8; CITRATE SYNTHASE, MITOCHONDRIAL; 1.
DR   Pfam; PF00285; Citrate_synt; 1.
DR   PRINTS; PR00143; CITRTSNTHASE.
DR   SUPFAM; SSF48256; Citrate synthase; 1.
DR   PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE   2: Evidence at transcript level;
KW   Mitochondrion; Reference proteome; Transferase; Transit peptide;
KW   Tricarboxylic acid cycle.
FT   TRANSIT         1..30
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000250"
FT   CHAIN           31..468
FT                   /note="Citrate synthase, mitochondrial"
FT                   /id="PRO_0000253909"
FT   ACT_SITE        303
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        349
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        404
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
SQ   SEQUENCE   468 AA;  51747 MW;  233F8FAF3D4BE6DE CRC64;
     MSLISAGRVC ARILGAKNSP CALIAARQAS SSTNLKDVLS DLIPKEQTRI KNFKQQHGKT
     VIGQVTVDMV YGGMRGMKGL VYETSVLDPD EGIRFRGYSI PECQKLLPKA PGGEEPLPEG
     LFWLLVTGEV PNQDQVNWIS KEWAKRAALP SHVVTMLDNF PTNLHPMSQL SAAITALNSE
     SNFARAYAEG VNKAKYWELV YEDSMDLIAK LPCVAAKIYR NLYREGSSIG AIDSNLDWSD
     NFTNMLGYTD QQFTELMRLY LTIHSDHEGG NVSAHTSHLV GSALSDPYLS FSAAMNGLAG
     PLHGLANQEV LVWLTSLQKD LGGEVSDEKL RDYIWNTLNS GRVVPGYGHA VLRKTDPRYT
     CQREFALKHL PDDPMFKLVA QLYKIVPNIL LEQGKAKNPW PNVDAHSGVL LQYYGMTEMN
     YYTVLFGVSR ALGVLSQLIW SRALGFPLER PKSMSTDGLM QLVGSKSG
//