Q7ZWV9 (AMPM1_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine aminopeptidase 1
Short name=MAP 1
Short name=MetAP 1
EC=3.4.11.18

Alternative name(s):
Peptidase M 1

Gene names

Name:

metap1

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Removes the amino-terminal methionine from nascent proteins By similarity.
Catalytic activity	Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Cofactor	Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions By similarity. Binds 1 sodium ion per subunit. The sodium ion has a structural role By similarity.
Sequence similarities	Belongs to the peptidase M24A family.
Sequence caution	The sequence AAH46685.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	Cobalt Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Gene Ontology (GO)
Biological process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 385	385	Methionine aminopeptidase 1		PRO_0000323738
Sites
	Metal binding	220	1	Cobalt 1 By similarity
	Metal binding	231	1	Cobalt 1 By similarity
	Metal binding	231	1	Cobalt 2 By similarity
	Metal binding	294	1	Cobalt 2 By similarity
	Metal binding	327	1	Cobalt 2 By similarity
	Metal binding	358	1	Cobalt 1 By similarity
	Metal binding	358	1	Cobalt 2 By similarity
	Binding site	203	1	Substrate By similarity
	Binding site	301	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7ZWV9 [UniParc]. Last modified March 18, 2008. Version 2. Checksum: E3CCA9B269376EEF	FASTA	385	43,196
	10 20 30 40 50 60 MAAVESRVCE TEGCSSEAKL QCPTCIKLGI QGSYFCSQEC FKGSWATHKL LHKKAKDDKI 70 80 90 100 110 120 KPEVSPWTMD GEVNTDPWPG YRYTGKLRPH YPLTPMRPVP SYIQRPDYAD HPLGMSESEQ 130 140 150 160 170 180 TLKGTSQIKT LSPEDIEGMR VVCRLAREVL GVAAMMVKSG ITTEEIDHAV HLACISRNCY 190 200 210 220 230 240 PSPLNYYNFP KSCCTSVNEV ICHGIPDRRP LQDGDIVNVD ITVYRDGYHG DLNETFYVGD 250 260 270 280 290 300 VDEGAKRLVE TTYECLMQAI DEVKPGVRYR ELGNIIQKHA QANGFSIVRS YCGHGIHKLF 310 320 330 340 350 360 HTAPNVPHYG KNKAVGVMKP GHVFTIEPMI CEGGWQDETW PDGWTAITRD GKRSAQFEHT 370 380 LLVTETGCEI LTCRLEENGR PYFIS

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC046685 mRNA. Translation: AAH46685.1. Different initiation. BC070567 mRNA. Translation: AAH70567.1.
UniGene	Xl.76058.
3D structure databases
HSSP	HSSP built from PDB template 2B3H based on UniProtKB P53582.
ProteinModelPortal	Q7ZWV9.
SMR	Q7ZWV9. Positions 81-385.
ModBase	Search...
Protein family/group databases
MEROPS	M24.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Xenbase	XB-GENE-6254194. metap1.
Phylogenomic databases
HOVERGEN	HBG067178.
Family and domain databases
InterPro	IPR001714. Pept_M24_MAP. IPR000994. Pept_M24_structural-domain. IPR002467. Pept_M24A_MAP1. [Graphical view]
Gene3D	G3DSA:3.90.230.10. Peptidase_M24_cat_core. 1 hit.
Pfam	PF00557. Peptidase_M24. 1 hit. [Graphical view]
PRINTS	PR00599. MAPEPTIDASE.
SUPFAM	SSF55920. Peptidase_M24_cat_core. 1 hit.
TIGRFAMs	TIGR00500. Met_pdase_I. 1 hit.
PROSITE	PS00680. MAP_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPM1_XENLA

Accession

Primary (citable) accession number: Q7ZWV9
Secondary accession number(s): Q6NRY8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 18, 2008
Last modified:	November 16, 2011
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program